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Our aim was to evaluate the effects of fish oil feeding on sperm classical parameters, level of reactive oxygen spices (ROS), spermatozoa death incidence and in vitro fertilization (IVF) rate in rams. We randomly assigned nine rams, into two experimental groups (isoenergetic and isonitrogenous rations with constant level of vitamin E supplement): control (CTR; n = 5) and fish oil (FO; n = 4, 35 g/day/ram). Diets were fed for 70 days during the physiological breeding season. After a 21‐day dietary adaptation period, semen was collected weekly from each ram by an artificial vagina. Sperm classical parameters were determined by the computer‐assisted sperm analyzer system (CASA), and it was prepared for IVF process by swim‐up technique. These evaluations were performed during the first and last weeks of sampling. Intracellular ROS level and spermatozoa death incidence were detected by flow cytometry on a weekly basis after adaptation. Data were analysed with SPSS 15. The volume, concentration (3.6 and 2.7 × 109/ml) and sperm progressive motility (60 and 48%) were significantly improved in the FO group compared with the CTR (p < 0.05). A comparison of two‐cell stage embryos following IVF in the two groups showed a significantly higher fertilization rate in the FO group (56%) compared with the CTR (49%). Superoxide anion (O2?) rate was significantly lower (p < 0.05) at the third week of sampling in the FO. Although the H2O2 rate was numerically lower in the FO group compared with the CTR, this difference was not significant. In addition, apoptosis showed a significant difference in the third week of sampling (15 and 30% for FO and CTR, respectively; p < 0.05). Overall, adding fish oil to the ram diet not only improved sperm quality and IVF results, it also could reduce oxygen‐free radicals and the incidence of spermatozoa death.  相似文献   
43.
Gesunde Pflanzen - A&nbsp;factorial split-plot experiment (2015–17) was carried out in a&nbsp;randomized complete block design with three replications at Karaj, Iran, to investigate...  相似文献   
44.

The decrease of genetic variation in crops like tomato (Solanum lycopersicum L.) due to domestication, breeding, and population bottle necks for fresh market consumptions has increased the need for this cash crop’s conservation and characterizations. To study the genetic variation of this valuable genetic resource in the National Plant Gene Bank of Iran, a collection of 589 tomato accessions from different regions was characterized using semi-automatic and high-throughput techniques. Based on the fruit shape, the studied accessions were classified into ten groups and significant differences for all the fruit characteristics studied indicated an abundant diversity within the germplasm collected from different regions. Studied tomato germplasm was grouped into nine main clusters based on hierarchical cluster analysis. More than 83% of data variation was explained by seven components in which the first two components explained for 50% of the variation where fruit shape index and proximal/distal fruit end shape showed a high contribution in the variation of the first component. This unique genetically divergent germplasm could be utilized to select tomato breeding lines of interest. Also, accessions belonged to the heterotic clusters could be selected for hybridization and conservation purpose as well as enrichment of pre-breeding programs of the tomato germplasm.

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A pot experiment was conducted in sandy clay loam saline-sodic soil to assess the effects of farm yard manure (FYM), municipal solid waste (MSW) composts and gypsum application on nitrate leaching, soil chemical properties and crop productivity under rice-wheat cropping system. It also aims at establishing the correlation between soil phsico-chemical properties and yield response using principle component analysis and Pearson correlation analysis. The MSW was decomposed aerobically, an-aerobically and co-composted. Maximum nitrate leaching was observed during rice (75.9 mg L?1) and wheat (37.2 mg L?1) with an-aerobically decomposed MSW as compared with control treatment. Results revealed a decrease in soil pH (?6.95% and ?8.77%), electrical conductivity (EC) (?48.13% and ?51.04%), calcium carbonate (CaCO3) (?40.30% and ?48.96%), and sodium adsorption ratio (SAR) (?40.27% and ?45.98%) with an-aerobically decomposed MSW compost during rice and wheat, respectively. In this treatment, organic matter (OM) (93.55% and 121.51%) and cation exchange capacity (CEC) (19.31% and 31.79%) were the highest as compared with control treatment during rice and wheat, respectively. Rice and wheat growth were significantly (p≤ 0.05) increased by an-aerobically decomposed MSW followed by co-compost, aerobically decomposed MSW, FYM, gypsum and control. Furthermore, Pearson correlation coefficients predicted significant positive correlation of yield with soil OM, and CEC while inverse relationship was observed with EC, pH, CaCO3, and nitrogen use efficiency. Soil amelioration with organic and gypsum amendments was further confirmed with principal component analysis. This study has proved an-aerobically decomposed MSW as an effective solution for MSW disposal, thereby improving soil chemical properties and crop productivity from sandy clay loam saline-sodic soil.  相似文献   
47.
Since 1995, landings of Atlantic Thread Herring (Opisthonema oglinum) on the Florida Panhandle averaged 124 mt each spring (April–June) but declined to nearly zero in 1996–97 and 2006. To elucidate causes of recurring periods of low landings, we used generalized additive mixed models (GAMMs) to examine the relationship between Atlantic Thread Herring catch in a trawl survey on the west‐central coast of Florida and four environmental variables. Chlorophyll‐a was significant in the models, indicating a direct relationship between local abundance of baitfish and their planktonic food. Temperature and depth were also significant whereas salinity was not. During 2003 and 2012, synoptic satellite‐derived temperature and chlorophyll‐a maps were used with the GAMMs to predict monthly spatial availability on the Florida Panhandle fishing grounds (long. 85–88o W and depth 12–25 m). The predicted monthly availability was significantly correlated with commercial catch rates (Pearson's r = 0.26, P = 0.004, d.f. = 118). We used multiple linear regression (MLR) with lags to describe the effect of river discharge and wind (as a transport mechanism) on surface chlorophyll‐a over the Panhandle fishing grounds. Discharge from local rivers was significant in all MLRs, and the Mississippi was only significant as an interaction with wind. We conclude that Atlantic Thread Herring are distributed over the Panhandle fishing grounds based on food availability that is driven by river discharge and eastward transport of Mississippi River plumes. This analysis improves our understating of baitfish dynamics, an important ecosystem component in the Gulf of Mexico.  相似文献   
48.
Glycated hemoglobin (HbG) concentration is a retrospective measure of mean blood glucose level and is not affected by recent stresses, food ingestion, or exercise. Although HbG has been determined in various wild and domestic animals such as kestrels, mankhor, mouflon, aoudad, deer, goat, sheep, dog, camel, ostrich, and horse, there is no information about diagnostic values of HbG as an indicator of blood glucose status in horses. The purposes of this study were to determine normal value of HbG in Iranian crossbred horses and to investigate its relation to fasting plasma glucose. Blood samples were collected from jugular veins of 193 clinically healthy adult crossbred horses (102 males and 91 females). After separation and washing of red blood cells, hemolysate was prepared and subjected to weak cation exchange chromatography for determination of HbG. Glucose was measured in fasting plasma samples. HbG percent (HbG%) in the studied horses was 3.21 ± 0.56 in males and 3.34 ± 0.72 in females. Fasting plasma glucoses were 81.3 ± 7.6 and 84.2 ± 14.5 mg/dL in males and females, respectively. HbG% and plasma glucose were highly correlated (r = 0.81, P < .01). We concluded that HbG% can be considered as a good indicator of blood glucose status in the horse. Two horses with abnormally higher HbG% were found in this study showing persistent high fasting plasma glucose. We concluded that HbG determination could be a more reliable indicator of basal blood glucose concentrations in horses.  相似文献   
49.
The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.  相似文献   
50.
The possibility to replace or reduce rotifer use with a microparticle diet (MPD) from first feeding in yellowfin seabream larvae was investigated. The experiment consisted of five treatments, a rotifer (100%) control treatment (100R), three experimental treatments, which received a fixed ration of MPD supplemented with 75%, 50% and 25% of the amount rotifer fed in the control treatment (treatments 75R‐MPD, 50R‐MPD and 25R‐MPD respectively), and finally a treatment with the fixed MPD ration only (treatment 0R‐MPD). The results indicated that feeding regimes 100R, 75R‐MPD and 50R‐MPD did not differ significantly in the total length, final survival and stress test resistance. The highest dry weight was achieved in treatments 100R and 75R‐MPD at the end of the experiment compared with treatments 50R‐MPD, 25R‐MPD and 0R‐MPD. The 0R‐MPD treatment showed the lowest growth and survival. Yellowfin seabream larvae were able to ingest inert food directly from the moment of first feeding.  相似文献   
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