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991.
Hordeum chilense Roem. et Schult. is a diploid wild South American barley that contains genes of interest for cereal breeding, many of them
located on chromosome 1Hch. In the current study, two H. chilense-wheat addition lines with deletions in the 1Hch chromosome were used for sub-arm localization of five prolamin (glutenin and gliadin) loci and 33 EST-SSR marker loci on
chromosome 1Hch. The two sets of markers were distributed across five sub-arm chromosome regions. Three glutenin loci (Glu-H
ch
2, Glu-H
ch
3, Glu-H
ch
4) together with the gliadin locus Gli-H
ch
1 were located on the distal 20% of the 1HchS arm, whereas the glutenin locus Glu-H
ch
1 was on the proximal 88% region of 1HchL. Among 33 EST-SSR marker loci, 7 (21.2%) were on the 1HchS arm and, of them, 3 (9.1%) were on the distal 20% end and 4 (12.1%) on the proximal 80% region. The 26 loci (78.8%) on 1HchL were distributed across three different regions: 18 (78.8%) in the proximal 88%, 3 (9.1%) in the distal 12% and 5 (15.2%)
in a region less than 12% from the distal end. The deletions in the 1Hch chromosome added to the common wheat background were thus shown to be useful for determining the sub-arm location of EST-SSR
and prolamin loci. This could facilitate the identification of molecular markers linked to genes of agronomic interest and
the isolation of such genes for use in common wheat improvement. 相似文献
992.
J.?Huerta-EspinoEmail author R.?P.?Singh S.?Germán B.?D.?McCallum R.?F.?Park W.?Q.?Chen S.?C.?Bhardwaj H.?Goyeau 《Euphytica》2011,179(1):143-160
Leaf rust caused by Puccinia triticina is the most common and widely distributed of the three wheat rusts. Losses from leaf rust are usually less damaging than
those from stem rust and stripe rust, but leaf rust causes greater annual losses due to its more frequent and widespread occurrence.
Yield losses from leaf rust are mostly due to reductions in kernel weight. Many laboratories worldwide conduct leaf rust surveys
and virulence analyses. Most currently important races (pathotypes) have either evolved through mutations in existing populations
or migrated from other, often unknown, areas. Several leaf rust resistance genes are cataloged, and high levels of slow rusting
adult plant resistance are available in high yielding CIMMYT wheats. This paper summarizes the importance of leaf rust in
the main wheat production areas as reflected by yield losses, the complexity of virulence variation in pathogen populations,
the role cultivars with race-specific resistance play in pathogen evolution, and the control measures currently practiced
in various regions of the world. 相似文献
993.
Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant
(Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 hybrid seed of some commercial varieties.
However, a comprehensive study of the variation of this useful trait among different materials of common eggplant and related
species is still lacking. In this work, we studied the androgenic response of 12 accessions of common eggplant and related
materials from the primary (eggplant complex) and secondary genepools. We cultured anthers of all the accessions under the
same experimental conditions, and studied their competence to produce calli, embryos and plants, as well as the quality and
origin of the embryos produced. In our conditions, anthers of 11 out of the 12 accessions produced somatic calli, whereas
only 5 also produced microspore-derived embryos, with variable results in terms of embryo quality and of frequency of embryo
induction and plant germination. Embryos of responding accessions were initially haploid, and reached the DH status, verified
with SSR markers, after a defined period of culture. In addition to other aspects common to many androgenesis-responsive species,
our results allowed us to extract conclusions particular to common eggplant and relatives, including the difficulty for finding
sources of androgenic competence out of S. melongena, the reduced impact of calli in the production of non-DH individuals, and the need to avoid the occurrence of severe anatomical
and functional problems in the apex of most embryos, which seriously reduces their germinative success. 相似文献
994.
995.
996.
Loredana Lunadei Pamela Galleguillos Beln Diezma Lourdes Lle Luis Ruiz-Garcia 《Postharvest Biology and Technology》2011,60(3):225-234
Increased regulation of chemical fumigants has forced the almond industry to seek alternatives for postharvest control of insect pests in raw almonds. This paper reports developments of non-chemical treatment for postharvest disinfestation of almonds using radio frequency (RF) energy. A pilot-scale 27 MHz RF unit was used to evaluate effects of a RF treatment protocol on quality attributes in treated in-shell and shelled almond samples. The RF treatment protocol used 0.75 kW RF power, a forced hot air at 63 °C, back and forth movements on the conveyor at 0.56 m/min, and single mixing, which all improved the final heating uniformity. RF treatments sharply reduced the heating time from 86 and 137 min for hot air heating to only 6.4 and 8.8 min for the center of 1.5 kg in-shell and 2.4 kg shelled almond samples to reach 63 °C, respectively. Almond quality was not affected by the RF treatments because peroxide values, fatty acid and kernel color of treated almonds were better than or similar to untreated controls after 20 d at 35 °C, simulating 2 years of storage at 4 °C. RF treatments did not significantly affect the kernel moisture content of both types of almonds but reduced the moisture content in the shell. RF treatments may hold great potential to replace chemical fumigation for disinfesting almonds. 相似文献
997.
C.M. Morvillo E.B. de la Fuente A. Gil M.A. Martínez-Ghersa J.L. González-Andújar 《European Journal of Agronomy》2011,34(4):211-221
Annual wormwood interference on soybean crop growth and yield may result from competition and allelopathy, which are modulated by crop management. Allelochemicals released by annual wormwood (e.g. artemisinin) may affect the crop directly or indirectly through the effect on the nitrogen fixing symbiont, Bradyrhizobium japonicum. The objectives were (i) to quantify the crop response (i.e. biomass production, nodulation and yield) to weed interference and (ii) to determinate the relative change of competition and allelopathy interferences, when a sublethal dose of herbicide is applied. Two split plot field experiments with three replications were used. The experiment involved a factorial combination of five weed–crop density (soybean/annual wormwood, plants m?2) levels: D1, pure soybean, 40/0 plants m?2; D2, 40/2 plants m?2; D3, 40/4 plants m?2 and D4, 40/8 plants m?2, and D5, pure annual wormwood, 0/8 plants m?2, two activated carbon (allelopathy) levels: C?, with activated carbon (reduced allelopathy) and C+, non activated carbon applied (with allelopathy) and two herbicide levels: H?, untreated and H+, treated with a sub-lethal dose of glyphosate. Activated carbon to adsorb allelochemicals (with and without activated carbon) and glyphosate application (with and no herbicide) were assigned to sub-plots. Increasing weed density did not affect crop biomass at flowering, but changed nodule number and soybean yield with a different pattern depending on carbon and herbicide treatment. Relative crop yield decreased with increasing relative weed biomass. This decrease was particularly drastic when allelopathy was reduced by activated carbon and without herbicide application. The maximum yield losses of 33% in 2006 and 17% in 2007 were observed with the highest weed density (8 plants m?2). In contrast, without carbon (high allelopathy level), soybean yield remained stable within the explored range of annual wormwood biomass, despite the fact that weed biomass at high densities (D4) was high enough to generate competition. The lack of response to increasing weed density could be related to the indirect effect of allelochemicals interacting with soil microorganisms (i.e. B. japonicum) that positively affected the nodulation (e.g. larger nodules in 2006 and increased nodules biomass due to higher number of roots in 2007 at high densities). With herbicide application, soybean yield of both carbon treatments remained stable when biomass of annual wormwood increased. This research provided strong evidence in support of the existence of positive effect of allelopathic and competitive interactions between annual wormwood and soybean crop under field conditions that may be overridden under herbicide application. 相似文献
998.
The Penman–Monteith equation for reference evapotranspiration (ETo) estimation cannot be applied in many situations, because climatic records are totally or partially not available or reliable.
In these cases, empirical equations that rely on few climatic variables are necessary. Nevertheless, the uncertainty associated
with empirical model estimations is often high. Thus, the improvement of methods relying on few climatic inputs as well as
the development of emergency estimation tools that demand no local climatic records turns into a task of great relevance.
The present study describes different approaches based on multiple linear regression, simple regression and artificial neural
networks (ANNs) to deal with ETo estimation exclusively from exogenous records from secondary stations. This cross-station approach is based on a continental
characterization of the study region, which enables the selection and hierarchization of the most suitable ancillary data
supplier stations. This procedure is compared with different traditional and cross-station approaches, including methodologies
that also consider local temperature inputs. The proposed methods are also evaluated as gap infilling procedures and compared
with a simple methodology, the window averaging. The artificial neural network and the multiple linear regression approaches
present very similar performance accuracies, considerably higher than simple regression and traditional temperature-based
approaches. The proposed input combinations allow similar performance accuracies as ANN models relying on exogenous ETo records and local temperature measurements. The cross-station multiple linear regression procedure is recommended due to
its higher simplicity. 相似文献
999.
1000.
CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the LacI-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-LacI-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-LacI. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function. 相似文献