Organic carbon characteristics in Swedish forest soil trace post‐depositional carbon dynamics          下载免费PDF全文

J. Routh  T. S. Bianchi  J. A. Hutchings  P. Kuhry  R. K. Ranjan 《European Journal of Soil Science》2016,67(4):492-503

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How methane feedback response influence redox processes in a tropical vertisol     

Santosh Ranjan Mohanty  Sneh Tiwari  Garima Dubey  Usha Ahirwar  Bharati Kollah 《Biology and Fertility of Soils》2016,52(4):479-490

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Cacti: notes on their uses and potential for climate change mitigation     

Pragya Ranjan  J. K. Ranjan  R. L. Misra  M. Dutta  B. Singh 《Genetic Resources and Crop Evolution》2016,63(5):901-917

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Effects of water exchange protocols on water quality,sedimentation rate and production performance of Penaeus monodon in earthen ponds          下载免费PDF全文

Rajeeb K Mohanty  Atmaram Mishra  Dileep K Panda  Dhiraj U Patil 《Aquaculture Research》2015,46(10):2457-2468

This study was carried out in farmers' fields to quantify the total water and consumptive water use in grow‐out culture of Penaeus monodon under recommended package of practice with two different water management protocols: T₁, with no water exchange and T₂, with regulated water exchange. Treatment‐wise estimated total water use, was 2.09 and 2.43 ha‐m 122 day^?1, while the computed consumptive water use index (m³ kg^?1 biomass) was 5.35 and 6.02 in T₁ and T₂ respectively. Lower rates of water exchange (T₂) showed significantly improved (P < 0.05) crop performance in terms of performance index (19.75 ± 0.75), production‐size index (74.1 ± 3.4), survival rate (80.13 ± 1.7%) and productivity (2.44 ± 0.08 t) over the zero water exchange. The shrimp pond water quality suitability index (WQSI) infers that regulated water exchange (T₂) improved the overall suitability of water quality for shrimp culture. WQSI up to 90 days of culture ranged between 7.5–9.0 in T₂, needs little management while in the last month of rearing, it was good with moderate management requirements. Treatment‐wise sediment load ranged between 50.4–56.3 m³ t^?1 shrimp biomass. High intensity of water exchange and low apparent feed conversion ratio influenced in lowering the sedimentation rate. Regulated water exchange protocol (T₂) performed well (higher net total water productivity and net consumptive water productivity) against no water exchange (T₁). A higher OV:CC ratio (ratio of the output value to the cost of cultivation) indicated that T₂ had a distinct edge over the T₁ protocol.  相似文献   

Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper,Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males          下载免费PDF全文

Ritesh Ranjan  Biji Xavier  Biswajit Dash  Loveson L Edward  Relangi D Suresh  Pilli Suresh Kumar 《Aquaculture Research》2015,46(9):2065-2072

This study aimed to test the efficacy of 17 α‐methyl testosterone (17 α‐MT) alone and in combination with letrozole, an aromatase inhibitor, for the induction of sex reversal in protogynous greasy grouper, Epinephelus tauvina. Further, the long‐lasting effects of these treatments and spawning performance of sex‐reversed males were also investigated. Greasy grouper with oocytes in the perinucleolus stage were implanted with 5 mg 17 α‐MT kg^?1 body weight (T₁), 5 mg 17 α‐MT and 0.2 mg letrozole kg^?1 body weight (T₂) and 5 mg 17 α‐MT with 0.4 mg letrozole kg^?1 body weight (T₃) and no androgens/enzyme inhibitor implanted (C). The 17 α‐MT alone and in combination of letrozole‐induced sex reversal in greasy grouper, whereas untreated control fish (C) showed normal ovarian development. However, T₂ and T₃ group showed 100% sex reversal and completion of spermatogenesis up to functional male phase in 2 and 3 months, respectively, whereas T₁ group resulted in only 66.67% functional male with motile spermatozoa after 4 months. Sex‐reversed males successfully fertilized the eggs during induced spawning. There were significant differences on fertilization and hatching rates between T₂ group (79.00 ± 4.36%; 77.67 ± 2.87%, respectively) and T₁ group (57.67 ± 3.17%; 63.87 ± 2.91%, respectively). The result suggested that 17 α‐MT (5.0 mg kg^?1 BW) in combination with letrozole (0.2 mg kg^?1 BW) has the potential to produce 100% sex‐reversed male in short period in greasy grouper, which might greatly help in seed production of greasy grouper.  相似文献   

The effect of plant growth regulators and natural supplements on in vitro propagation of Pogostemon cablin Benth.     

Mallappa Kumara Swamy  Sudipta Kumar Mohanty  Maniyam Anuradha 《Journal of Crop Science and Biotechnology》2014,17(2):71-78

The effect of plant growth regulators and natural supplements on the morphogenetic response of Pogostemon cablin Benth. was investigated. Murashige and Skoog (MS) media supplemented with 0.5 mg L^?1 benzyl-6-adenine and 0.5 mg L^?1 kinetin was effective in inducing multiple shoots (63.20 ± 0.15) with an average shoot length of 5.27 ± 0.15 cm and biomass of 5.20 ± 0.10 g shoot^?1. Among the natural supplements, 10% coconut water supplemented to MS media showed a better response in all the morphological parameters studied. The use of 10% tomato extract, 20% banana extract, 10% carrot extract, and 10% papaya extract in MS medium have efficiently increased multiple shoots, shoot length, and fresh weight of the shoots. The natural supplements also effectively increased the chlorophyll content, total protein, and total carbohydrate content in the plant. The frequency of rooting (93%) was highest when shoots were implanted on 1/2 strength MS media with 100 mg L^?1 activated charcoal. The in vitro rooted plants were successfully acclimatized and established in soil. Also, RAPD analysis showed no variation suggesting true-to-type nature of the micropropagated plants. Hence, this protocol can effectively reduce the cost of in vitro multiplication of plants.  相似文献   

Cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in Cirrhinus mrigala infected with Aeromonas hydrophila     

Sahoo PK  Mohanty BR  Kumari J  Barat A  Sarangi N 《Comparative immunology, microbiology and infectious diseases》2009,32(6):527-537

Transferrin partial complementary DNAs were cloned from the livers of five species in four genera of Indian carps (Indian major carp species: Labeo rohita, Catla catla and Cirrhinus mrigala; medium carp: Puntius sarana; minor carp: Labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cDNA sequences. The partial transferrin cDNAs of the five species of carps had sizes from 624 to 633 bp (487 bp for L. rohita) and encoded an open reading frame consisting of 206–211 (162 for L. rohita) amino acids. The alignments of carp cDNA sequences showed 85–97% homology and 71–93% homology in deduced amino acid sequences. A phylogenetic tree of amino acid sequences of transferrin cDNAs from carps showed that the relationship among the four genera of Indian carps is well correlated with that derived from classic morphologic analyses. The hypothesized cleavage site and interdomain bridge of transferrin molecule were predicted for the above carp species and interestingly the cleavage site amino acid sequence was found to be conserved among all the carps. To study the tissue-specific expression of the transferrin gene, various tissues (liver, kidney, spleen, brain, muscle, testis, heart, intestine, gill and fin) from apparently healthy (control), moribund and survived C. mrigala experimentally infected with Aeromonas hydrophila infection were analyzed. Transferrin mRNA was detected only in liver RNA and to lesser extent in brain tissue out of the 10 tissues analyzed irrespective of bacterial infection.  相似文献   

Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758)     

Saurav Kumar  R. P. Raman  Kundan Kumar  P. K. Pandey  Neeraj Kumar  B. Mallesh  Snatashree Mohanty  Abhay Kumar 《Fish physiology and biochemistry》2013,39(4):733-747

Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1–5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L^?1 as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2 % DMSO solution without azadirachtin and considered as negative control T0^?. Along with six treatment groups, a positive control T0⁺ of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L^?1 and that also shifted haematological and serum biochemical parameters towards homeostasis.  相似文献   

Induction,purification and partial characterization of vitellogenin in an Indian major carp Catla catla (Ham.)     

Bipulendu Jena  Jyotirmaya Mohanty  Radha C Das  Sushil K Garnayak  Samiran Nandi 《Aquaculture Research》2013,44(12):1901-1911

Vitellogenin was purified from the serum of 17‐β estradiol (E₂)‐induced juvenile Catla catla using a simple two‐step purification procedure i.e. selective chemical precipitation followed by gel filtration chromatography. Purified protein migrated as single band in a native gradient PAGE which indicated the purity of the sample. The molecular weight of the native catla vitellogenin (~440 kDa) was determined using gel filtration chromatography. In SDS‐PAGE under reducing conditions catla vitellogenin dissociated into three major sub units at 115 kDa, 102 kDa and 73 kDa along with a few faint bands. Confirmation of purified protein as catla vitellogenin was supported by multiple physiological evidences, e.g. absence in male as well as juvenile sera and presence in matured female fish, ability to be synthesized upon estradiol injection in immature fish and certain unique biochemical properties like high molecular weight, phospholipoglycoprotein nature of the molecule. Western blot analysis showed that polyclonal antibody raised against purified protein detected vitellogenin in the sera of catla and in a few species selected from Cyprinidae family. These antisera were used to detect vitellogenin in liver tissue of hormone‐induced catla using immunohistochemistry and its applicability in other immunoassays is discussed.  相似文献   

Effects of feed restriction on compensatory growth performance of Indian major carps in a carp–prawn polyculture system: a response to growth depression          下载免费PDF全文

R.K. Mohanty 《Aquaculture Nutrition》2015,21(4):464-473

Effects of feed restriction on compensatory growth (CG) performance of Indian major carps, sediment loading and water productivity in a carp–prawn grow‐out production system were examined. The overall growth and crop performance were in the similar line in both T₁ (regular feeding, 2 times a day) and T₂ (4‐week feeding followed by 2‐week no feed). However, between T₁ and T₃ (8‐week feeding followed by 2‐week no feed), there was a significant (P < 0.05) variation in the overall growth and crop performance. This was probably due to the longer refeeding periods after cyclic food deprivation that successfully triggered compensatory growth response in T₃ (CG Index: 98–104%). Treatment‐wise sediment load ranged between 59.2 and 69.6 m³ t^?1 biomass. Higher the apparent feed conversion ratios, higher was the sedimentation rate. Higher the feed input, higher were the water exchange requirement, total water use and consumptive water use index. Cyclic food deprivation and refeeding also helped in maintaining water quality due to the restricted feed input (10.5% in T₂ and 2.0% in T₃), thus minimizes the input cost and improves production efficiency. Keeping the growth and yield performance, water productivity and economic efficiency in view, T₃ is considered the best feed management protocol followed by T₂ and T₁.  相似文献