全文获取类型
收费全文 | 294篇 |
免费 | 27篇 |
国内免费 | 1篇 |
专业分类
林业 | 17篇 |
农学 | 8篇 |
基础科学 | 1篇 |
33篇 | |
综合类 | 40篇 |
农作物 | 8篇 |
水产渔业 | 54篇 |
畜牧兽医 | 134篇 |
园艺 | 19篇 |
植物保护 | 8篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 6篇 |
2020年 | 14篇 |
2019年 | 10篇 |
2018年 | 17篇 |
2017年 | 15篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 13篇 |
2013年 | 8篇 |
2012年 | 10篇 |
2011年 | 26篇 |
2010年 | 15篇 |
2009年 | 13篇 |
2008年 | 15篇 |
2007年 | 18篇 |
2006年 | 24篇 |
2005年 | 18篇 |
2004年 | 14篇 |
2003年 | 11篇 |
2002年 | 20篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有322条查询结果,搜索用时 156 毫秒
81.
Feed Containing Novacq Improves Resilience of Black Tiger Shrimp,Penaeus Monodon,to Gill‐associated Virus‐induced Mortality
下载免费PDF全文
![点击此处可从《Journal of the World Aquaculture Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Melony J. Sellars Min Rao Nick Polymeris Simon J. Irvin Jeff A. Cowley Nigel P. Preston Brett D. Glencross 《Journal of the World Aquaculture Society》2015,46(3):328-336
The ability of Novacq to improve resilience of black tiger shrimp, Penaeus monodon, to infection and mortality induced by gill‐associated virus (GAV) was investigated. Over a 26‐d period, shrimp were fed pellets with or without 10% Novacq. Following this, four replicate tanks, each containing 10 shrimp that had been fed either diet, were maintained as‐is, injected with saline or injected with GAV inoculum (i.e., 40 shrimp for each of the six groups). For shrimp (n = 20) in two of each group of four tanks, survival was monitored daily over 14 d and a pleopod was sampled from each shrimp on Days 0 and 14. For the other two tanks, a pleopod was sampled from each shrimp on Days 0, 3, 7, 10, and 14 to track changes in GAV loads over time. Survival was significantly higher (P < 0.05) from Day 7 onward among the group fed Novacq. GAV infection loads appeared to vary more between individuals in the Novacq diet cohort, but overall were not reduced significantly at any time points post‐challenge compared to shrimp tested from the Control diet cohort. 相似文献
82.
83.
84.
Lliso I Tadeo FR Phinney BS Wilkerson CG Talón M 《Journal of agricultural and food chemistry》2007,55(22):9047-9053
In this work, major protein changes in the albedo of the fruit peel of Murcott tangor (tangerine x sweet orange) during postharvest ageing were studied through 2D PAGE. Protein content in matured on-tree fruits and in fruits stored in nonstressing [99% relative humidity (RH) and 25 degrees C], cold (99% RH and 4 degrees C), and drought (60% RH and 25 degrees C) conditions was initially determined. Protein identification through MS/MS determinations revealed in all samples analyzed the occurrence of manganese superoxide dismutase (Mn SOD), actin, ATP synthase beta subunit (ATPase), citrus salt-stress associated protein (CitSap), ascorbate peroxidase (APX), translationally controlled tumor protein (TCTP), and a cysteine proteinase (CP) of the papain family. The latter protein was identified in two different gel spots, with different molecular mass, suggesting the simultaneous presence of the proteinase precursor and its active form. While Mn SOD, actin, ATPase, and CitSap were unchanged in the assayed conditions, TCTP and APX were downregulated during the postharvest ageing process. Ageing-induced APX repression was also reversed by drought. CP contents in albedo, which were similar in on- and off-tree fruits, were strongly dependent upon cold storage. The active/total CP protein ratio significantly increased after cold exposure. This proteomic survey indicates that major changes in protein content in the albedo of the peel of postharvest stored citrus fruits are apparently related to the activation of programmed cell death (PCD). 相似文献
85.
Reddy V Nathues A Le Corre L Sierks H Li JY Gaskell R McCoy T Beck AW Schröder SE Pieters CM Becker KJ Buratti BJ Denevi B Blewett DT Christensen U Gaffey MJ Gutierrez-Marques P Hicks M Keller HU Maue T Mottola S McFadden LA McSween HY Mittlefehldt D O'Brien DP Raymond C Russell C 《Science (New York, N.Y.)》2012,336(6082):700-704
Multispectral images (0.44 to 0.98 μm) of asteroid (4) Vesta obtained by the Dawn Framing Cameras reveal global color variations that uncover and help understand the north-south hemispherical dichotomy. The signature of deep lithologies excavated during the formation of the Rheasilvia basin on the south pole has been preserved on the surface. Color variations (band depth, spectral slope, and eucrite-diogenite abundance) clearly correlate with distinct compositional units. Vesta displays the greatest variation of geometric albedo (0.10 to 0.67) of any asteroid yet observed. Four distinct color units are recognized that chronicle processes--including impact excavation, mass wasting, and space weathering--that shaped the asteroid's surface. Vesta's color and photometric diversity are indicative of its status as a preserved, differentiated protoplanet. 相似文献
86.
Yun MK Wu Y Li Z Zhao Y Waddell MB Ferreira AM Lee RE Bashford D White SW 《Science (New York, N.Y.)》2012,335(6072):1110-1114
The sulfonamide antibiotics inhibit dihydropteroate synthase (DHPS), a key enzyme in the folate pathway of bacteria and primitive eukaryotes. However, resistance mutations have severely compromised the usefulness of these drugs. We report structural, computational, and mutagenesis studies on the catalytic and resistance mechanisms of DHPS. By performing the enzyme-catalyzed reaction in crystalline DHPS, we have structurally characterized key intermediates along the reaction pathway. Results support an S(N)1 reaction mechanism via formation of a novel cationic pterin intermediate. We also show that two conserved loops generate a substructure during catalysis that creates a specific binding pocket for p-aminobenzoic acid, one of the two DHPS substrates. This substructure, together with the pterin-binding pocket, explains the roles of the conserved active-site residues and reveals how sulfonamide resistance arises. 相似文献
87.
SB Grant JD Saphores DL Feldman AJ Hamilton TD Fletcher PL Cook M Stewardson BF Sanders LA Levin RF Ambrose A Deletic R Brown SC Jiang D Rosso WJ Cooper I Marusic 《Science (New York, N.Y.)》2012,337(6095):681-686
Humans create vast quantities of wastewater through inefficiencies and poor management of water systems. The wasting of water poses sustainability challenges, depletes energy reserves, and undermines human water security and ecosystem health. Here we review emerging approaches for reusing wastewater and minimizing its generation. These complementary options make the most of scarce freshwater resources, serve the varying water needs of both developed and developing countries, and confer a variety of environmental benefits. Their widespread adoption will require changing how freshwater is sourced, used, managed, and priced. 相似文献
88.
89.
Dolente BA Beech J Lindborg S Smith G 《Journal of the American Veterinary Medical Association》2005,227(7):1134-1141
OBJECTIVE: To evaluate risk factors associated with development of catheter-associated jugular thrombophlebitis in hospitalized horses. DESIGN: Retrospective case-control study. ANIMALS: 50 horses with thrombophlebitis and 100 control horses. PROCEDURE: Medical records from 1993 through 1998 were searched for horses with thrombophlebitis. Horses that were hospitalized for at least 5 days, had an i.v. catheter placed in a jugular vein (other than for solely anesthetic purposes), and had no evidence of thrombophlebitis during admission or hospitalization were chosen as controls. Signalment, history, clinicopathologic findings, primary illness, and treatment were obtained from the medical records. Data were analyzed by use of logistic regression to perform univariate and multivariate analyses. RESULTS: For a horse with endotoxemia, the odds of developing thrombophlebitis were 18 times those for a similar horse without endotoxemia. For a horse with salmonellosis, the odds of developing thrombophlebitis were 68 times those for a similar horse without salmonellosis. For a horse with hypoproteinemia, the odds of developing thrombophlebitis were almost 5 times those for a similar horse without hypoproteinemia. For a horse in the medicine section, the odds of developing thrombophlebitis were 16 times those for a similar horse in the surgery section. For a horse with large intestinal dise, the odds of developing thrombophlebitis were 4 times those for a similar horse without large intestinal disease. For a horse receiving antidiarrheal or antiulcerative medications, the odds of developing thrombophlebitis were 31 times those for a similar horse not receiving these medications. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that patient factors, including large intestinal disease, hypoproteinemia, salmonellosis, and endotoxemia, were associated with development of catheter-associated thrombophlebitis in horses. 相似文献
90.
Cobb SP Hogg RA Challoner DJ Brett MM Livesey CT Sharpe RT Jones TO 《The Veterinary record》2002,150(1):5-8
A large outbreak of suspected botulism occurred on a dairy farm. The affected animals were listless and showed signs ranging from hindlimb unsteadiness to lateral recumbency, although the most common presentation was sternal recumbency with an apparent hindlimb weakness when stimulated to rise. Postmortem examinations revealed no conclusive gross pathology or histopathology. The affected cattle were found to have neutrophilia and hyperglycaemia with no other consistent haematological or biochemical abnormalities. The combination of clinical signs, disease epidemiology and the ruling out of other differential diagnoses strongly supported a diagnosis of unconfirmed botulism; however, the source of toxin was not demonstrated. Botulism is a severe disease in human beings and there are uncertainties about the pharmacokinetics and pharmacodynamics of Clostridium botulinum toxins. In such circumstances, a precautionary approach to food safety is essential. Restrictions were placed on the movement of livestock and sale of milk from the farm premises until 14 days after the onset of the last clinical case. 相似文献