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51.
Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet 总被引:1,自引:0,他引:1
Cao H Hininger-Favier I Kelly MA Benaraba R Dawson HD Coves S Roussel AM Anderson RA 《Journal of agricultural and food chemistry》2007,55(15):6372-6378
Green tea has antidiabetic, antiobesity, and anti-inflammatory activities in animal models, but the molecular mechanisms of these effects have not been fully understood. Quantitative real-time polymerase chain reaction (PCR) was used to investigate the relative expression levels and the effects of green tea (1 and 2 g solid extract/kg diet) on the expression of glucose transporter family genes (Glut1/Slc2a1, Glut2/Slc2a2, Glut3/Slc2a3, and Glut4/Slc2a4) and insulin signaling pathway genes (Ins1, Ins2, Insr, Irs1, Irs2, Akt1, Grb2, Igf1, Igf2, Igf1r, Igf2r, Gsk3b, Gys1, Pik3cb, Pik3r1, Shc1, and Sos1) in liver and muscle of rats fed a high-fructose diet known to induce insulin resistance and oxidative stress. Glut2 and Glut4 were the major Glut mRNAs in rat liver and muscle, respectively. Green tea extract (1 g) increased Glut1, Glut4, Gsk3b, and Irs2 mRNA levels by 110, 160, 30, and 60% in the liver, respectively, and increased Irs1 by 80% in the muscle. Green tea extract (2 g) increased Glut4, Gsk3b, and Pik3cb mRNA levels by 90, 30, and 30% but decreased Shc1 by 60% in the liver and increased Glut2, Glut4, Shc1, and Sos1 by 80, 40, 60, and 50% in the muscle. This study shows that green tea extract at 1 or 2 g/kg diet regulates gene expression in the glucose uptake and insulin signaling pathway in rats fed a fructose-rich diet. 相似文献
52.
Wade T. Walker Robert J. Callan Ashley E. Hill Kelly B. Tisher 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(8):765-771
This study evaluated the effects of administering oral powder electrolytes on packed cell volume (PCV), plasma chemistry parameters, and incidence of colic in horses participating on a 6-day 162-km trail ride in which water was not offered ad libitum. Twenty-three horses received grain with powder electrolytes daily while 19 control horses received grain only. Horses were ridden approximately 32 km a day at a walk or trot. Packed cell volume and plasma chemistry parameters were analyzed daily. Episodes of colic were diagnosed and treated by a veterinarian unaware of treatment group allocation. Blood parameters and incidence of colic were compared between treatment groups. Electrolyte administration did not alter PCV or plasma chemistry parameters compared to controls. The incidence of colic was significantly higher in treated horses (P = 0.05). Oral powder electrolytes did not enhance hydration status or electrolyte homeostasis and may be associated with colic in horses participating on long distance trail rides similar to this model. 相似文献
53.
K. R. Kelly B. H. Pypendop K. L. Christe 《Journal of veterinary pharmacology and therapeutics》2014,37(5):480-485
This study reports the pharmacokinetics of buprenorphine in conscious rhesus macaques (Macaca mulatta) after intravenous (i.v.) and intramuscular (i.m.) administration. Four healthy, opioid‐naïve, socially housed, adult male macaques were used. Buprenorphine (0.03 mg/kg) was administered intravenously as a bolus or intramuscularly on separate occasions. Blood samples were collected prior to, and up to 24 h, postadministration. Serum buprenorphine concentrations were analyzed with liquid chromatography–mass spectrometry. Noncompartmental pharmacokinetic analysis was performed with commercially available software. Mean residence time in the i.v. study as compared to the i.m. study was 177 (159–189) vs. 185 (174–214) min, respectively [median (range)]. In the i.v. study, concentration back‐extrapolated to time zero was found to be 33.0 (16.8–57.0) ng/mL [median (range)]. On the other hand, the maximum serum concentration found in the i.m. study was 11.8 (6.30–14.8) ng/mL [median (range)]. Rhesus macaques maintained concentrations >0.10 ng/mL for over 24 h in the i.v. study and over 12 h in the i.m. study. Bioavailability was found to be 68.1 (59.3–71.2)% [median (range)]. No significant adverse effects were observed in the monkeys at the 0.03 mg/kg dose of buprenorphine during either study. 相似文献
54.
Kelly W. Wilcox Joachim Stoss Edward M. Donaldson 《Aquaculture (Amsterdam, Netherlands)》1984,40(1):77-87
Egg content originating from ruptured ova progressively reduced the success of fertilization in coho salmon (Oncorhynchus kisutch) eggs. The salmon egg differs in ionic composition from the ovarian fluid in exhibiting higher K+ and Mg2+ and lower Na+ concentrations. Normal ovarian fluid induced spermatozoan motility whereas ovarian fluid contaminated with egg content progressively lost the ability to induce motility. Loss of motility was related to decreasing ratios in the ovarian fluid, indicating that reduced fertility may be caused by inactivation of sperm cells in the insemination medium. Removal of contaminated ovarian fluid by rinsing ova in isotonic media induced spermatozoan motility and restored fertility completely. The use of an isotonic NaHCO3 solution for rinsing of ova is suggested as a routine procedure for artificial insemination in salmonids where the presence of broken eggs is suspected. 相似文献
55.
A hydraulically integrated serial turbidostat algal reactor (HISTAR) for the mass production of microalgae was designed, constructed and preliminarily evaluated. The 9266-l experimental system consists of two enclosed turbidostats hydraulically linked to a series of six open continuous-flow, stirred-tank reactors (CFSTRs). The system was monitored and controlled using GENESIS process control software. A production study was preformed using Isochrysis sp. (C-iso) to assess system stability and production potential under commercial-like conditions. The study was performed at the following target system parameters: system dilution rate of 0.49 per day, pH 7.6, NITROGEN=10 mg l−1, PHOSPHORUS=2 mg l−1, and artificial illumination (photosynthetic photon flux density) from 1000 W metal halide LAMPS=800 μmol s−1 m−2. At steady state conditions, daily harvested algal paste was 1454 g (wet), mean areal system PRODUCTIVITY=47.8±3.04 g m−2 per day (17.1±1.09 g C m−2 per day) and mean CFSTR6 DENSITY=105.5±6.71 mg l−1. 相似文献
56.
A bench-scale, micro-computer automated algal turbidostat was constructed and its production and culture maintenance capabilities were evaluated. The computer program "Supervisor," written in Turbo Pascal, consisted of a series of functions and procedures integrated through a core program consisting of a: 1) stack; 2) bubble sort routine; and 3) supervisor procedure. The program controlled the daily routine procedures including harvesting, feeding, monitoring and a semimonthly disinfection process. Data received from the monitoring devices were used by the computer for decision making processes concerning changes in operation. In addition to reducing labor, continuous computer monitoring insured more reliable system operation.
The system operated successfully for five months, reliably producing Chlorella minutissima . For each harvesting event, the percent volume removed from each chamber depended on the solar cell reading recorded immediately before the harvest cycle began. Significant correlations between millivolts and algal biomass concentrations allowed the use of solar cells as a reliable and inexpensive method of culture density management. Kinetic studies using Chlorella minutissima resulted in a maximum biomass concentration of approximately 190 g/m3 and an average specific growth rate of 0.66/d. Experimental data combined with a first order computer analysis projected a daily harvest of 82 g/m3 /d (dry weight) based on a 38% turnover rate, the average observed harvest rate for the three chambers. 相似文献
The system operated successfully for five months, reliably producing Chlorella minutissima . For each harvesting event, the percent volume removed from each chamber depended on the solar cell reading recorded immediately before the harvest cycle began. Significant correlations between millivolts and algal biomass concentrations allowed the use of solar cells as a reliable and inexpensive method of culture density management. Kinetic studies using Chlorella minutissima resulted in a maximum biomass concentration of approximately 190 g/m
57.
An Erratum has been published for this article in Aquatic Conservation: Marine and Freshwater Ecosystems 12(2), 2002 577
- 1. This research extends techniques of predictive mapping from their application in terrestrial environments to marine landscapes by investigating the relationship between seagrass and hydrodynamics in Core Sound, North Carolina, USA.
- 2. An empirically derived logistic multiple regression model and a Boolean logic suitability model were used to produce several predictive map products, including: susceptibility of seagrasses to storms, probability of seagrass cover, and suitability of areas for restoration of seagrasses. A visual comparison between these maps and conventional seagrass polygon maps allows for a discussion of ‘field’ versus ‘object’ mapping, and the ramifications for management based on different cartographic techniques.
- 3. The predictive method used here showed that only a small portion (19%) of the seagrass bed in the study area would be expected to have a high probability of seagrass coverage. The majority of the seagrass habitat in the study area was predicted to have less than 50% probability of seagrass cover. In addition, 16% of the nearly 2000 ha of seagrass within the study area were predicted to be highly susceptible to acute storm events. Moreover, using a conservative set of site selection criteria, only 7% of the study area encompassed by seagrass habitat was predicted to have a high probability of successful restoration if injured.
- 4. This method provides for an inexpensive way to scale‐up from high‐resolution data to a coarser scale that is often required for conservation and management.
58.
Increased abundances of a number of seabird species have been found in areas of mussel longline aquaculture (compared to control
sites) in a recent study at Bantry Bay, southwest Ireland. The aim of this study was to investigate whether this form of mussel
aquaculture also affects the activity budgets and foraging behaviour of these seabirds. Scan-sampling was used to compare
seabird activity budgets between three areas of mussel longline aquaculture and three control sites. Foraging activities of
Great-Northern Divers Gavia immer, an Annex 1 species under the Euopean Union's Birds Directive, were also compared between sites using focal sampling. The
behaviour of gulls (Larus sp.), Cormorants Phalacrocorax carbo, Shags Phalacrocorax aristotelis and Great-Northern Divers (but not auks, Alcidae) differed significantly between areas of mussel aquaculture and control
sites in this study. Mussel suspension buoys were heavily utilized by gulls, cormorants and shags as safe perching platforms
for preening activities. Gulls also fed extensively on epifauna attached to the buoys. Great-Northern Divers spent more time
snorkelling (head submerged in search of food) in mussel sites than in the control sites, however dive duration and visible
foraging success rates did not differ significantly between sites. Overall, the environmental impact of mussel suspension
culture appears to be positive or neutral on marine bird species occurring at this study site. 相似文献
59.
60.