全文获取类型
收费全文 | 1145篇 |
免费 | 78篇 |
专业分类
林业 | 45篇 |
农学 | 32篇 |
基础科学 | 9篇 |
149篇 | |
综合类 | 120篇 |
农作物 | 27篇 |
水产渔业 | 92篇 |
畜牧兽医 | 685篇 |
园艺 | 18篇 |
植物保护 | 46篇 |
出版年
2023年 | 16篇 |
2022年 | 9篇 |
2021年 | 33篇 |
2020年 | 29篇 |
2019年 | 34篇 |
2018年 | 31篇 |
2017年 | 23篇 |
2016年 | 35篇 |
2015年 | 27篇 |
2014年 | 28篇 |
2013年 | 56篇 |
2012年 | 41篇 |
2011年 | 64篇 |
2010年 | 35篇 |
2009年 | 37篇 |
2008年 | 51篇 |
2007年 | 56篇 |
2006年 | 53篇 |
2005年 | 55篇 |
2004年 | 52篇 |
2003年 | 44篇 |
2002年 | 38篇 |
2001年 | 21篇 |
2000年 | 26篇 |
1999年 | 11篇 |
1998年 | 13篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1993年 | 10篇 |
1992年 | 12篇 |
1991年 | 11篇 |
1990年 | 16篇 |
1989年 | 10篇 |
1988年 | 16篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 15篇 |
1984年 | 9篇 |
1983年 | 11篇 |
1980年 | 8篇 |
1979年 | 12篇 |
1978年 | 16篇 |
1977年 | 9篇 |
1976年 | 8篇 |
1975年 | 8篇 |
1973年 | 14篇 |
1970年 | 9篇 |
1967年 | 6篇 |
1966年 | 7篇 |
排序方式: 共有1223条查询结果,搜索用时 437 毫秒
991.
OBJECTIVE: To report use of a modified Whitehouse approach in standing horses for management of inspissated guttural pouch empyema. STUDY DESIGN: Retrospective study. ANIMALS: Adult horses (n=10) with guttural pouch empyema. METHODS: Inspissated exudate in 1 or both guttural pouches was removed surgically through a modified Whitehouse approach, with the horses standing and sedated. Medical records of affected horses were reviewed to determine history; physical, endoscopic, and radiological examination findings; surgical technique; complications, and outcome. RESULTS: All horses had purulent nasal discharge; 3 horses had dysphagia, 2 had recurrent laryngeal neuropathy on the side affected by guttural pouch empyema, and 1 had persistent soft palate displacement. Inspissated exudate was removed safely without causing apparent discomfort. Eight horses returned to their previous level of athletic activity after surgery; 1 horse dysphagic before surgery, was euthanatized because of persistent dysphagia after surgery, and 1 horse died 1 week after surgery for unknown reasons. Streptococcus equi subsp equi was isolated from the affected guttural pouch of 3 horses. CONCLUSIONS: Inspissated exudate can be removed surgically from the guttural pouch in standing horses through a modified Whitehouse approach. CLINICAL RELEVANCE: To eliminate risks associated with general anesthesia and avoid surgical suite contamination, removal of chondroids can be performed in standing sedated horses through a modified Whitehouse approach. 相似文献
992.
Hakimuddin F Paliyath G Meckling K 《Journal of agricultural and food chemistry》2006,54(20):7912-7923
Food components influence the physiology by modulating gene expression and biochemical pathways within the human body. The disease-preventive roles of several fruit and vegetable components have been related to such properties. Polyphenolic components such as flavonoids are strong antioxidants and induce the expression of several xenobiotic-detoxifying enzymes. The mechanism of selective cytotoxicity induced by red grape wine polyphenols against MCF-7 breast cancer cells was investigated in relation to their interference with calcium homeostasis. MCF-7 cells showed an increase in cytosolic calcium levels within 10 min of treatment with the polyphenols. Immunohistochemical localization of calmodulin with secondary gold-labeled antibodies showed similar levels of gold labeling in both MCF-7 cells and the spontaneously immortalized, normal MCF-10A cell line. MCF-7 cells treated with the red wine polyphenol fraction (RWPF) showed swelling of endoplasmic reticulum, dissolution of the nucleus, and loss of plasma membrane integrity as well as reduced mitochondrial membrane potential. These cells were arrested at the G2/M interphase. By contrast, MCF-10A cells did not show such changes after RWPF treatment. The results suggest that polyphenol-induced calcium release may disrupt mitochondrial function and cause membrane damage, resulting in selective cytotoxicity toward MCF-7 cells. This property could further be developed toward breast cancer prevention strategies either independently or in conjunction with conventional prevention therapies where a positive drug-nutrient interaction can be demonstrated. 相似文献
993.
995.
996.
Assessing the extraction and quantification of amorphous silica in soils of forest and grassland ecosystems 总被引:2,自引:0,他引:2
L. Saccone D. J. Conley E. Koning D. Sauer M. Sommer D. Kaczorek S. W. Blecker & E. F. Kelly 《European Journal of Soil Science》2007,58(6):1446-1459
Many studies have highlighted the importance of the Amorphous Silica (ASi) pool to the overall mass balance in the biogeosphere. In order to advance our knowledge of measurements and quantification of this pool, it is necessary to compare the ability of different extractants to dissolve ASi in soils and to test methods developed in the aquatic sciences to soils systems. The methods used in this work included three acid extraction techniques (0.2 m NH4‐oxalate, 0.1 m NH4‐citrate and 0.5 m NH4‐acetate) and two alkaline extraction techniques (0.094 m Na2CO3 and 0.5 m NaOH), which are more commonly used for the measurement of ASi in aquatic sediments. Our results indicate that the amount of Si extracted from phytolith samples with the acid methods was two orders of magnitude less than the amount of extracted by alkaline extractions. When applied to natural soil samples, these extractions show that the acid techniques are only able to extract loosely‐bound components such as adsorbed Si and Si bound in amorphous matrices with Al and Fe. While Na2CO3 or NaOH extracted the same amount of ASi in Podzols, Na2CO3 was able to extract only part of the ASi extracted with NaOH in Chernozems. Pre‐treatment of the samples with 0.1 m HCl before the Na2CO3 extraction did not increase amounts of ASi extracted. The present work suggests that alkaline methods used commonly for ASi on aquatic sediment samples can be used on a wide variety of soils. 相似文献
997.
Johnson KS 《Journal of agricultural and food chemistry》2005,53(26):10120-10126
To evaluate the prooxidant versus antioxidant properties of plant phenolics toward leaf-feeding caterpillars, quenching of the stable ABTS radical by five phenolics was measured in two physiological contexts: hemolymph and midgut fluid. Addition of tannic acid, chlorogenic acid, quercetin, or catechin to Manduca sexta (L.) gut fluid increased its total antioxidant capacity by 12-45%, with tannic acid and quercetin being the most powerful in this regard. The antioxidant contribution of the phenolics increased with longer (30-60 min) incubation time in gut fluid. Chlorogenic acid and caffeic acid exhibited the weakest antioxidant activity in gut fluid. The total antioxidant capacity of hemolymph is considerably less than that of gut fluid, and in hemolymph chlorogenic and caffeic acids sometimes acted as mild prooxidants, particularly after longer incubation periods (30-60 min), although this trend was not statistically significant. Tannic acid, catechin, and quercetin behaved as antioxidants in hemolymph. These results suggest that many phenolics have radical scavenging activity in the digestive tract, but some may have more detrimental effects after absorption into the hemolymph compartment. 相似文献
998.
O'Connell JE Kelly AL Fox PF de Kruif KG 《Journal of agricultural and food chemistry》2001,49(9):4424-4428
An explanation as to how casein micelles dissociate when heated in the presence of ethanol is presented. Dissociation of casein micelles in milk-ethanol mixtures was studied using (1)H NMR, and the effects of addition of CaCl(2), NaCl, or EDTA or alteration of milk pH on this dissociation were studied. It is proposed that at low temperatures, ethanol reduces the solvent quality of milk serum, but above a critical temperature (approximately 30 degrees C in a 35% ethanol solution), ethanol enhances solvent quality and dissociates the casein micelles. Ethanol reduced protein hydrophobicity and increased the pK(a) value of phosphoserine, effects that are likely to be significant in the dissociating effect of ethanol at elevated temperatures. 相似文献
999.
A freshwater wetland at the Experimental Lakes Area in northwestern Ontario stored most of the SO4 2? received annually from precipitation, runoff and experimental additions. The S budget was determined for a small fen spray irrigated with H2SO4 and HNO3. Annual S retention was greatest during the first year of experimental addition of H2SO4 (73% of input in 1983). Retention was lowest (22%) in 1984, a year of lower than average precipitation with a long hot summer. During years with hot, dry summers, SO4 2? was produced from the reoxidation of reduced S compounds in the peat and released to surface waters. The autumn SO4 2? pulse was accompanied by the release of Ca and Mg but was not accompanied by a H+ release as has been detected in eastern Ontario and southern Norway, areas which receive more acidic precipitation. 相似文献
1000.
Vincent L. St.Louis John W. M. Rudd Carol A. Kelly Leonard A. Barrie 《Water, air, and soil pollution》1995,80(1-4):405-414
Concentrations of methyl mercury (MeHg) and total mercury (THg) in precipitation were measured at the Experimental Lakes Area (ELA), a remote field station in northwestern Ontario. We found that precipitation was a source of both MeHg and THg to boreal ecosystems, but at lower rates than in industrialized regions of North America and Scandinavia. MeHg concentrations in precipitation ranged from 0.010 to 0.179 ng L?1 and were highest when events originated west of the ELA. THg concentrations in precipitation ranged from 0.95 to 9.31 ng L?1 and were highest when the events came from the southeast. There was no relationship between THg and MeHg over time in precipitation. Inputs of both MeHg and THg to ecosystems were highest during summer months. 相似文献