人工改造野生大豆GsDREB2基因对植物耐盐和耐渗透胁迫能力的影响   总被引：1，自引：0，他引：1  

才华  孙娜  宋婷婷 《草业学报》2018,27(6):168-176

DREB (dehydration responsive element binding protein)转录因子是一个干旱应答元件的结合蛋白,它能特异结合启动子中含有DRE/CRT顺式元件,激活逆境诱导基因的表达,调控植物对干旱、低温、高盐、高温等胁迫的耐逆性。大量研究表明DREB转录因子在信号传导、作用机理及基因表达方面存在复杂性。为了野生大豆来源GsDREB2基因能更有效地发挥功能,人工突变该基因的负向调节结构域(negative regulatory domain, NRD,140~204),经改造命名为GsDREB2-mNRD。在酵母中比较全长基因(FLDREB2)和GsDREB2-mNRD转录激活和与DRE元件结合的能力,并验证GsDREB2-mNRD核定位情况。分别将FLDREB2和GsDREB2-mNRD转化拟南芥,通过拟南芥幼苗期盐胁迫和渗透胁迫试验,比较GsDREB2-mNRD和FLDREB2在提高植物耐盐和渗透胁迫方面的差异。结果表明,GsDREB2基因内部存在着负向调节结构域(NRD),抑制了GsDREB2转录激活功能和DRE元件结合的特性;经改造的GsDREB2基因依然能定位在细胞核;超量表达GsDREB2-mNRD基因的拟南芥耐盐和渗透胁迫能力明显强于非转基因对照,也高于FLDREB2基因超表达的拟南芥;野生大豆来源的GsDREB2基因NRD结构域的缺失可增强该基因在植物耐盐、渗透胁迫等逆境胁迫下的功能。  相似文献   

水氮供应对地下滴灌紫花苜蓿生长特征及草地小气候的影响     

胡伟  张亚红  李鹏  刘瑞  蔡伟  王小菊 《草业学报》2018,27(12):122-132

为探讨不同水氮处理条件下紫花苜蓿生长状况与草地小气候特征的关系,以2年生紫花苜蓿“巨能7号”为研究对象,采用田间试验和室内分析相结合的方法,研究了宁夏引黄灌区地下滴灌条件下不同滴灌量和施氮量处理下紫花苜蓿生长特征和草地小气候的变化。结果表明:1)滴灌量和施氮量对紫花苜蓿的株高、叶面积和鲜草产量都有显著的影响,表现为紫花苜蓿的株高、叶面积和鲜草产量均随滴灌量和施氮量的增加而增加,当施氮量增加到一定值时,继续增施氮肥,其鲜草产量增产效果在不同滴灌量处理下表现出不同的趋势。2)与不施氮处理相比,增施氮肥降低了紫花苜蓿株间空气温度、浅层土层温度和株间光照强度,而增加了群体内部空气相对湿度。3)不同滴灌量对紫花苜蓿的生长微环境的调节作用不同,随着滴灌量的增加,紫花苜蓿群体相对湿度逐渐提高,而紫花苜蓿株间气温和浅层土层温度降温效应越明显。4)紫花苜蓿生育期间株高与叶面积、草产量和群体内部相对湿度呈极显著正相关(P<0.01),与群体内光照强度、株间气温、浅层土壤温度呈极显著负相关(P<0.01)。合理减少滴灌量和施氮量不仅能维持紫花苜蓿良好的生长特征,而且能提高鲜草产量和改善草地生态环境条件。本研究旨在为紫花苜蓿群体微环境生态因子的改善及高产优质栽培措施提供科学依据。  相似文献   

与菜用大豆收割机配套的种植密度筛选试验     

许映君  蔡娜丹  陈纪算  冯洁琼 《长江蔬菜》2017,(8)

对2个菜用大豆品种毛豆3号、浙鲜豆8号进行了适用收割机采收的种植密度试验,分别比较了植株性状、豆荚和产量构成性状、产量。试验结果表明,适宜于毛豆3号、浙鲜豆8号收割机采收的种植密度为4100~4400穴/667 m~2。  相似文献   

亚洲棉GaPP2C24基因的克隆及表达分析     

蔡肖  甄军波  刘琳琳  刘迪  唐丽媛  张素君  李兴河  王海涛  刘存敬  张香云  张建宏  迟吉娜 《华北农学报》2020,35(3):33-39

PP2C是一大类重要的蛋白磷酸酶,广泛参与不同的逆境胁迫响应。为了解PP2C基因在干旱响应中的功能,以亚洲棉石系亚1号为材料,利用RT-PCR方法从中克隆了GaPP2C24基因,并对该基因进行了生物信息学分析,荧光定量PCR研究了其在亚洲棉不同组织和干旱胁迫下的表达情况。结果表明,GaPP2C24基因CDS序列全长为1 251 bp,编码416个氨基酸。序列同源比对发现,GaPP2C24与其他植物的PP2C氨基酸序列有较高的相似性,其中与可可树(EOY33930.1)、黄麻(OMO91132.1)的相似性分别为85%,84%。进化分析表明,GaPP2C24与同属锦葵目的黄麻聚在一支,亲缘关系最近,与可可树的亲缘关系次之。实时荧光定量PCR结果表明,GaPP2C24基因在子叶、下胚轴、胚根、叶、茎和根均有表达,且在茎中的表达量最高,根中次之。GaPP2C24受干旱胁迫的诱导表达,其在根和叶片中的相对表达量在处理1 h后最高,分别达到对照的53.9,6.9倍,推测该基因在棉花干旱响应中有重要作用。  相似文献   

江苏省森林碳储量的区域分布研究     

杨加猛  杜丽永  蔡志坚  张智光 《中南林业科技大学学报(自然科学版)》2014,(7):84-89

运用因子连续函数法对江苏省13个地级市的森林碳储量及其变化情况进行了估算。结果表明:2010年,江苏省森林碳储量为4 615.55×104 t,苏北、苏南和苏中的占比分别为57.83%、28.26%和13.91%;2005~2010年,江苏省森林碳储量增加了2 025.59×104 t,森林"碳汇"效应显著,但各区域的碳储量变化不均衡。在江苏新增造林面积空间较为有限的背景下,提高林地生产力,优化树种和林龄结构是增加森林碳储量的有效途径。  相似文献   

牛病毒性腹泻病毒E2蛋白纳米抗体筛选及反应原性检测     

李岩  肖盛中  杨艳  张彦红  蔡卓轩  周子恒  张哲  盛金良 《畜牧与兽医》2021,(3):71-76

通过噬菌体展示技术筛选牛病毒性腹泻病毒(BVDV)重组E2蛋白特异性纳米抗体,验证纳米抗体反应原性。使用BVDV灭活疫苗免疫羊驼,分别在第0、21、49及70天采集全血,测得抗体效价后分离全血中淋巴细胞,提取总RNA,反转录后PCR扩增目的片段。目的片段和pCANTAB5E使用限制性内切酶酶切连接后转至TG1感受态细胞中,应用噬菌体展示技术构建VHH噬菌体展示文库。再经过3轮"吸附-洗脱-筛选"后得到与BVDV-E2结合的噬菌体,用ELISA鉴定其反应性。结果获得插入率为90.8%,库容为1.02×107 CFU/mL的文库。ELISA结果和序列分析显示,得到2条与E2蛋白具有良好反应性的纳米抗体且与VHH同源性较高的序列。研究结果为BVDV的防控和新型疫苗的研制奠定基础。  相似文献   

Influence of paricalcitol on renal tubulointerstitial fibrosis in diabetic nephropathy     

WANG Lai-liang  LUO Qun  CAI Ke-dan  ZHOU Fang-fang  GAO Yan-hong 《园艺学报》2015,31(4):719-724

AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis.  相似文献   

杂交糯玉米新品种渝科糯1号的选育   总被引：1，自引：0，他引：1  

易红华  蔡治荣  陈荣丽  张胜恒 《湖南农业科学》2011,(5)

渝科糯1号是以B4301为母本,S181为父本,根据西南气候特点及种植习惯选育成的超高产、优质、适应性广的鲜食及加工兼用型糯玉米新品种.该品种2007年通过重庆市审定,2009年通过四川审定,2010年通过国家审定.  相似文献   

Clinical significance of KLF15 expression in human lung adenocarcinoma     

LIU Xiao-yan  ZHOU Li-neng  ZHANG Lei  CAI Xing-dong 《园艺学报》2017,33(8):1411-1416

AIM: To explore the clinical significance of Krüpple-like factor 15 (KLF15) protein expression in the patients with lung adenocarcinoma for exploring the therapeutic and prognositic biomarkers of lung cancer. METHODS: Four cases of lung adenocarcinoma tissues and matched adjacent tissues were collected from our hospital, and the expression of KLF15 protein in these tissues was analyzed by Western blot. At the same time, 72 cases of archived paraffin-embedded samples and clinical data of the patients with lung adenocarcinoma were also collected. The KLF15 protein expression in the archived paraffin-embedded lung adenocarcinoma samples was detected by immunohistochemical staining. The correlations between KLF15 protein expression and clinical characteristics of the patients including prognosis were also analyzed. In addition, the KLF15 protein was up-regulated in A549 cells, and then the effects of KLF15 protein on the viability of the cells were measured by CCK-8 assay. RESULTS: The protein expression of KLF15 in the 4 cases of lung adenocarcinoma tissues was significantly lower than that in matched paracancerous tissues. Fifty-three cases of lung adenocarcinoma specimens showed low expression or no expression of KLF15 protein in total 72 cases (73.6%). The 5-year survival rate of the patients with high expression of KLF15 protein in their specimens was higher than that of the patients with the low expression of KLF15 protein (P<0.01), and the expression of KLF15 protein was significantly correlated with the pathological staging (P<0.01) and T stage (P<0.01) of the patients with lung adenocarcinoma. Furthermore, the low expression of KLF15 protein was an important poor prognostic indicator of the patients. Up-regulation of KLF15 protein in the A549 cells significantly inhibited the growth of the cells. CONCLUSION: KLF15 inhibits the growth of lung adenocarcinoma cells. It could be used as a therapeutic target and a prognostic biomarker for the patients with lung adenocarcinoma.  相似文献   

年轻群体在公共绿地中的活动参与度调查——以上海浦东新区部分公园为例     

蔡伟勇  罗震伟 《安徽农业科学》2016,(12)

公共绿地作为开放空间,公众可以共享自然环境及社会资源。而当下公共绿地中不同群体的参与度存在较大差异,尤其是年轻群体参与度低。笔者以上海浦东新区部分公共绿地中年轻群体活动参与度为研究对象,从年轻群体的低参与度、活动意愿及管理者角度等方面,分析了年轻群体低参与度的原因,认为原因主要是年轻群体缺乏业余时间、绿地可达距离较远、活动内容缺乏等。在调查基础上,从活动载体、信息渠道、服务方式等方面提出建议,主张公共绿地管理者转变服务理念、开拓有益活动、建立信息平台、优化空间资源,以提高社会服务效能。  相似文献