双环蘑菇的遗传学特性及品种选育研究进展     

蔡为明  金群力  冯伟林  范丽军  李南羿 《食用菌学报》2007,14(4):76-86

Advances in research on Agaricus bitorquis mating and breeding systems,and the breeding of new strains of this mushroom both in China and overseas,have been reviewed.Progress in our understanding of the genetic basis of homokaryotic fruiting,nuclear migration and mitochondrial inheritance,has been presented.The three main groups of A.bitorquis strains (temperate,bridging and tropical) categorized on the basis of mating behavior,and the distribution of homogenic and heterogenic incompatibility within the groups,have been discussed.Existing problems have been identified,and proposals made for future research.  相似文献   

砂糖橘上碎叶病毒外壳蛋白基因的克隆及序列分析     

孟祥春  向旭  钟云  刘岩  易干军 《果树学报》2007,24(6):796-798

利用RT-PCR技术对广东砂糖橘碎叶病毒的外壳蛋白(CTLV-CP)基因进行了克隆和测序分析。DNA序列分析表明,砂糖橘CTLV-CP基因的cDNA序列全长为786bp,编码262个氨基酸。BLAST分析结果显示,所得序列与苹果、梨茎沟病毒(ASGV)外壳蛋白基因及其它柑橘CTLV-CP基因的同源性在87%～91%,证明所克隆的片段为CTLV-CP基因。  相似文献   

Diploid female gametes induced by colchicine in Oriental lilies   总被引：1，自引：0，他引：1  

Hongzhi Wu  Sixiang Zheng  Yueqiu He  Guijun Yan  Yufen Bi  Youyong Zhu 《Scientia Horticulturae》2007

The young flower buds of diploid Oriental cultivars: ‘Con. Amore’ and ‘Acapulco’ (Lilium) were treated with 0.02, 0.05, 0.1, and 0.2% colchicine to induce diploid egg (female gamete). The treated young buds between the two cultivars were crossed reciprocally as female parents with normal pollen respectively. The polyploid plants were identified by measuring stoma size and chromosome number. The results showed: that triploid progenies could be obtained through this way, and the fact that the treated young flower buds were successfully used as female parents indicated the formation of 2n or 2x egg cells. The above results implied that polyploidization by artificially induced diploid female gametes could be a powerful method to create novel variations in the breeding of Oriental lilies.  相似文献   

Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers     

Li-Wang Liu  Yan Wang  Yi-Qin Gong  Tong-Min Zhao  Guang Liu  Xiao-Yan Li  Fan-Min Yu 《Scientia Horticulturae》2007

Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed genetic purity of two commercial hybrid tomato (Lycopersicon esculentum L.) cultivars ‘Hezuo 903’ and ‘Sufen No. 8’. Genomic DNA from the two F₁ hybrid cultivars and their corresponding parental lines was screened with 218 RAPD decamer primers, 54 ISSR primers and 49 SSR primers. Among the 321 primers, 4 primers for ‘Hezuo 903’ and 3 for ‘Sufen No. 8’, which could produce both female and male parent-specific markers, were selected for testing the genetic purity. A total of 210 hybrid individuals of each cultivar were analyzed using the identified primers. The combined results of the marker analysis showed that eight of the 210 F₁ plants in ‘Hezuo 903’ and 13 of 210 in ‘Sufen No. 8’ were false hybrids, and the overall genetic purity of the two F₁ hybrid seed lots was 96.2 and 93.8%, respectively. This study showed that RAPD and SSR markers could provide a practical and efficient tool in quality control of the tomato commercial hybrid seeds.  相似文献   

Analysis of chromosome aberration due to ethidium bromide using AFM     

WU Yang-zhe  CAI Ji-ye 《园艺学报》2007,23(12):2451-2454

AIM: To study chromosome aberration due to ethidium bromide (EB),a heterocyclic organic compound and an organic fluorescence dye commonly used in biochemical experiment,and to help further understanding the molecular mechanism of tumor or cancer induced by EB and other heterocyclic organic compounds.METHODS: The toxicity action of EB was evaluated from three aspects including DNA,chromosome and embryo stem cells (ESCs) using atomic force microscopy (AFM),and thereinto,the morphology structural difference of ESCs treated with two EB doses was also valuated.RESULTS: The morphological structures of DNA,chromosome and ESCs were dramatically damaged.The average height of DNA decreased 0.5 nm;chromosomal arms were ruptured from centromere location;molecules of cellular membrane congregated and loop-like structure formed,and ES cell masses were collapsed and became dead after large EB doses treatment and mesh-like morphological structure was discernable.CONCLUSION: The toxicity action of EB is strong and destroys the surface structure of DNA and chromosome.EB induces structural aberration of ES cellular membrane and cell death.The results indicate that the action of EB is externalized at gene level and cell level,which is important to study the carcinogenicity of EB.  相似文献   

The anticancer activity of genistein on implanted tumor of human primary gastric carcinoma cells in nude mice     

ZHOU Hai-bo  CAI Jian-ting  DU Qin  CHEN Jin-ming△ 《园艺学报》2007,23(12):2379-2384

AIM: To investigate the apoptosis of implanted tumor of primary human gastric cancer cells in nude mice induced by genistein and the relation between this apoptosis and expression of bcl-2 and bax.METHODS: Establishing a transplanted tumor model by injecting human primary gastric cancer cells into subcutaneous tissue of nude mice.The different doses of genistein (0.5mg/kg,1mg/kg and 1.5 mg/kg ) were directly injected beside tumor body respectively,for six times at an interval of two days.Then changes of tumor volume were measured continuously and tumor inhibition rate of each group was calculated.We observed the morphologic alteration by electron microscope,measured the apoptotic rate by TUNEL staining method,detected the expression of apoptosis-regulated gene bcl-2 and bax by immunohistochemical staining and RT-PCR.RESULTS: Genistein could significantly inhibit carcinoma growth when it was injected near the carcinoma.Genistein induced implanted tumors cells to undergo apoptosis with apoptotic characteristics by transmission electron microscope.The apoptosis index of above three groups was increased progressively.Positive rate of Bcl-2 protein of above three groups was decreased progressively and positive rate of Bax protein of above three groups was increased progressively by immunohistochemical staining.The density of bcl-2 mRNA decreased progressively and the density of bax mRNA increased progressively with elongation of time by RT-PCR.CONCLUSION: Genistein is able to induce the apoptosis of transplanted tumor cells.This apoptosis may be mediated by down-regulating bcl-2 and up-regulating bax mRNA and its protein.  相似文献   

Lethal effect of VEGFR₂shRNA on HL60 cells in vitro using lentiviral vector     

GUO Hai-xia  LI Wen-yi  XU Ling  SU Hao-bin  LI Yang  XIA Yan  LIANG Li-yang 《园艺学报》2007,23(6):1053-1057

AIM: To look for harmfulless anti-leukemia drug with selective high performance, lethal effect of small hairpin RNA (shRNA) on VEGFR₂ gene expression of tumor cell line HL60 in vitro.METHODS: The most effective VEGFR₂ siRNA was designed and screened. The shRNA oligo was designed and pU6/VEGFR₂ entry clone was constructed. HL60 was transfected transiently and vascular endothelial growth factor receptor 2(VEGFR₂) expression was tested with MTT assay, RT-PCR and Western blotting. The expression clone was constructed and cotransfected with ViraPowerTM Packaging Mix into 293FTTM cells to produce Lentiviral vectors harboring Lenti6/shVEGFR₂. The virion supernatant was added into HL60 cells and VEGFR₂ gene inhibitory effect was determined. RESULTS: The inhibitory rates of VEGFR₂ siRNA c were high. VEGFR₂ expression in HL60 was inhibited by using pU6/VEGFR₂ entry clone constructed with shRNA and pENTRTM/U6. For HL60 cells, the inhibitory rate was 84.9%. The expression of VEGFR₂ mRNA and protein decreased significantly. 48 hours after transfection of pU6/shVEGFR₂ entry clone and transduction of Lenti6/shVEGFR₂ expression clone, the cell inhibitory rates were similar. Cell growth inhibitory rate of entry clone descended rapidly after this time point, the expression clone changed slowly, reaching the peak at 96 hours, dropped slightly, having no significance deviation. CONCLUSION: in vitro, VEGFR₂ shRNA using lentiviral vector blocks VEGF/VEGFR₂ self-secretion in HL60 cells, which inhibits leukemia development.  相似文献   

Xaf1 regulates TNFR signal pathway and induces apoptosis     

XIA Yan  TAN Wei-ping  LIANG Li-yang  SU Hao-bin  GUO Hai-xia  FANG Jian-pei  HUANG Shao-liang 《园艺学报》2007,23(7):1347-1351

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Establishment and evaluation of carotid air-dry endothelial denudation and regeneration model with intimal hyperplasia in mouse     

TAN Hong-mei  ZHAO Ming-qi  WU Wei-kang  SUN Juan  LI Yan  LUO Han-chuan  LIANG Tian-wen 《园艺学报》2007,23(10):2071-2073

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Expression of AFP and MXR7 mRNA in the peripheral blood and its relationship with prognosis of patients with hepatocellular carcinoma following liver transplantation     

YI Shu-hong  WANG Gen-shu  CAI Chang-jie  LU Min-qiang  YANG Yang  HU Bin  CHEN Gui-hua 《园艺学报》2007,23(11):2158-2163

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