菌渣还田对生菜生长、土壤养分及酶活性的影响研究   总被引：1，自引：1，他引：0  

滕青  林慧凡  曾梦凤  谢雨晴 《中国农学通报》2020,36(6):30-36

为考查菌渣的肥效及其对生态环境的影响,布置生菜栽培盆栽实验,研究施用菌渣还田对生菜生长、土壤养分、酶活性及微生物的影响。试验空白、菌渣、复合化肥、菌渣+复合化肥4个处理。结果表明：（1）施用菌渣,能促进青椒生长,但对生菜的生长促进不及化肥。（2）施加肥料能显著提高土壤养分,与空白处理相比,施纯菌渣对土壤全氮增加58.6%,碱解氮增加73.3%,硝态氮增加35.3%。（3）施菌渣能显著提高土壤酶活性和土壤微生物数量,施纯菌渣处理的土壤过氧化氢酶活性、脱氢酶活性和脲酶活性分别是化肥处理的1.31、1.40、1.44倍;土壤细菌、放线菌和真菌数量分别为化肥处理的1.56、1.72、1.72倍。施用菌渣可提高农田土壤养分、有机质含量、土壤酶活性和微生物数量,减少养分流失,改善农田土壤生态环境,具有良好的推广应用价值。  相似文献   

Effects of Rapeseed Green Manure on Soil Fertility and Bacterial Community in Dryland Wheat Field   总被引：2，自引：0，他引：2  

LI WenGuang  YANG XiaoXiao  HUANG ChunGuo  XUE NaiWen  XIA Qing  LIU XiaoLi  ZHANG XiaoQi  YANG Si  YANG ZhenPing  GAO ZhiQiang 《中国农业科学》2019,52(15):2664-2677

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木耳菌糠袋栽滑菇配方研究   总被引：1，自引：0，他引：1  

王芳  贾静  王军英  叶云霞  杨杰  孟俊龙  常明昌 《中国食用菌》2011,30(3):21-23

本试验采用不同比例的木耳菌糠替代部分木屑袋栽滑菇,以培养料（木屑89%、麦麸10%、石膏1%）为对照,探讨木耳菌糠栽培滑菇的可行性。结果表明,木耳菌糠添加量25%～45%时,可缩短发菌天数,其中菌糠添加量为45%时,满袋天数仅需52 d,并且长势较好;但菌糠添加量为55%～65%时,则延长了发菌天数,而且菌丝稀疏,长势也弱。对照（CK）与木耳菌糠添加量是25%～45%时,子实体生长良好,并且出菇整齐;菌糠添加量为55%～65%,则出菇不整齐。CK的生物学效率最高,但配方4（菌糠45%、木屑34%、麦麸10%、石膏1%）的生物学效率与CK差异不大,从成本和生态效益考虑,配方4栽培滑菇具有可行性,并已在广灵县进行推广。  相似文献   

库车坳陷克深地区现今地应力场及储层裂缝数值模拟          下载免费PDF全文

雷刚林  戴俊生  吴超  王珂  李青  王媛  何巧林  吴庆宽 《勤云标准版测试》2014,34(2):31-36

克深三维区目前处于勘探开发的前期,需要明确地应力场及地下储层裂缝的分布规律,指导开发工作,但该区存在地层埋深大、取心困难、成像测井资料缺乏等实际情况,应力场研究及裂缝预测难度大。利用有限元分析方法,以井点现今地应力值为约束进行了克深三维区现今地应力场的数值模拟,同时建立了应力应变与裂缝参数的定量关系模型,利用古今应力场结合法对克深三维区的裂缝参数进行了预测,模拟结果显示构造高部位和断层带是低应力值区及裂缝的有利发育区。裂缝的数值模拟结果与测井解释结果对比表明,预测结果与实钻吻合度较高,裂缝预测效果较好。  相似文献   

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL     

XIA Zi-rong  LI Qing  XIA Zhen  LI Ju-xiang  HONG Kui  WU Yan-qing  WU Qin-hua  CHENG Xiao-shu 《园艺学报》2017,33(12):2238-2244

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.  相似文献   

国内外主要畜产品与饲料价格分析简     

王春梅  杨林杰  常生华  侯扶江 《草业学报》2014,23(1):300-311

 我国正处于食物结构转型期，畜产品需求增长强劲，但畜产品和饲料价格持续上涨且容易受其他因素影响。通过分析２００８年９月－２０１３年８月国内、国际主要畜产品与饲料价格的变化规律、相关性和Ｇｒａｎｇｅｒ因果关系，结果表明，国内外畜产品和饲料价格整体呈波动上升趋势，且“食草型”畜产品价格高于“耗粮型”畜产品价格，国内主要畜产品和饲料价格均高于国际；除猪肉和牛肉价格外，畜产品和饲料价格的波动幅度整体是国内小于国际；国内猪肉和牛肉价格波动幅度较大，国际是“食草型”畜产品波动幅度较大；国际大豆价格是国内大豆价格变化的原因；国内牛肉价格和羊肉价格是大豆价格变化的原因、国内牛肉价格是豆粕价格变化的原因、玉米价格是鸡肉价格变化的原因，国际牛肉价格是大豆价格变化的原因。由此可见，农业政策、节日效应和饮食习惯等是影响畜产品价格的主导因素；因此，发展草地农业是稳定畜产品价格和确保畜产品安全的有效途径；同时，加大对农牧户的直补力度，并为其生产提供政策支持和服务。  相似文献   

绿皮苦瓜新品种冠春5号的选育     

李志  苗明军  杨亮  李跃建  肖平  常伟 《中国蔬菜》2017,1(12):66-68

冠春5号是以高代自交系327作母本,以自交系186作父本配制而成的苦瓜一代杂种。植株生长势旺盛,早熟,从播种至始收82 d(天)。主侧蔓均可结果,主蔓第1雌花节位为第7~8节。瓜长棒形,瓜长25~35 cm,横径5~7 cm,肉厚0.9~1.0 cm,单瓜质量420 g左右。果表皮浅绿色,点状突起与短条状突起相间瘤状物,尾部钝,果肉脆,苦味淡。早春栽培一般每667 m~2产量约3 000 kg,田间对白粉病和疫病的抗性优于对照新农村油绿苦瓜。适宜四川、重庆等地种植。  相似文献   

Foliar nematode,Litylenchus crenatae ssp. mccannii,population dynamics in leaves and buds of beech leaf disease‐affected trees in Canada and the US     

Sharon E. Reed  Sylvia Greifenhagen  Qing Yu  Adam Hoke  David J. Burke  Lynn K. Carta  Zafar A. Handoo  Mihail R. Kantor  Jennifer Koch 《Forest Pathology》2020,50(3)

A foliar nematode, Litylenchus crenatae ssp. mccannii, is associated with beech leaf disease (BLD) symptoms. Information about the types of tissues parasitized and how nematode populations fluctuate in these tissues over time is needed to improve surveys as well as understand the nematodes role in BLD. During this study, the nematode was detected throughout the known range of BLD by researchers at both Canadian and US institutions using a modified pan method to extract nematodes. Monthly collections of symptomatic and asymptomatic leaves during the growing season (May–October), and leaves and buds between growing seasons (November–March), revealed that nematodes were present in all tissue types. Progressively larger numbers of nematodes were detected in symptomatic leaves from Ohio and Ontario, with the greatest detections at the end of the growing season. Smaller numbers of nematodes were detected in asymptomatic leaves from BLD‐infected trees, typically at the end of the growing season. The nematode was detected overwintering in buds and detached leaves. The discovery of small numbers of nematodes in detached leaves at one location before BLD was detected indicates that nematodes may have been present before disease symptoms were expressed. Other nematodes, Plectus and Aphelenchoides spp., were infrequently detected in small numbers. Our findings support the involvement of the nematode in BLD and indicate that symptoms develop only when certain requirements, such as infection of buds, are met. We also found that the nematode can be reliably detected in buds and leaves using the modified pan extraction method.  相似文献   

卵型家族蛋白转录因子AtOFP8的抗旱性功能分析     

刘守银  唐尧  韩佳良  常缨 《华北农学报》2020,35(5):98-106

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NaCl对不同抗性水稻氮代谢及相关基因表达的影响     

杨德翠  徐青  赵方贵  柳冕  刘新 《华北农学报》2020,35(2):187-195

为了探究盐胁迫下不同抗性水稻氮代谢的变化,以抗性品种东稻4号、盐敏感品种日本晴为材料,测定NaCl胁迫下水稻根和叶中氮含量、氮代谢相关酶活及相关基因表达的变化。结果表明,0.3%的NaCl胁迫后水稻根和叶中NO~-_3含量、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、依赖NADH的谷氨酸合酶(NADH-GOGAT)活性均有不同程度的下降,而NH~+_4含量、依赖Fd的谷氨酸合酶(Fd-GOGAT)、谷氨酸脱氢酶(GDH)、天冬氨酸转氨酶(AST)活性均升高,东稻4号氮含量及氮代谢酶活性比日本晴稳定,根和叶中变化一致。检测氮转运及代谢相关基因表达发现,硝酸根转运蛋白基因OsNRT1;1、OsNRT1;5、OsNRT1;8、OsNRT2;1,铵转运蛋白基因OsAMT1;1、OsAMT1;2及谷氨酰胺合成酶基因OsGS1;2、天冬氨酸转氨酶基因OsAS盐胁迫后在2个品种中均升高,在高表达部位升高显著;谷氨酸合酶基因OsFd-GOGAT在两品种叶中升高显著,根中稍有下降;谷氨酸合酶基因OsNADH-GOGAT和谷氨酸脱氢酶基因OsGDH在日本晴根中升高显著,在叶中及东稻4号中升高但不显著。OsNR1在NaCl胁迫后两品种叶中表达量显著降低,根中无差异。东稻4号OsNRTs及氮代谢相关基因表达量总体上高于日本晴,而日本晴OsAMTs升高幅度大于东稻4号。研究表明,NaCl胁迫下,东稻4号相比日本晴更能保持氮代谢稳定性,这可能是水稻抗性品种耐盐的一个重要机制。  相似文献