Brazilian foot and mouth disease status and meat exportation to the European Union     

Luiz Felipe Ramos Carvalho  Cristiano Barros de Melo  Luiza Seixas  Concepta McManus 《Tropical animal health and production》2014,46(3):495-502

The aim of this study was to define the differences between the Brazilian states that export and do not export meat to the European Union (EU) and to identify the variables that are important to meet the export requirements. Infrastructure and computerization of the control of animal transit in Brazil that impact on regional health status were evaluated and linked to other variables such as status for foot and mouth disease (FMD) and qualification to export meat to EU. Variables related to transit control of bovines implemented by the state agencies of animal health and inspection in each Brazilian state were evaluated. Using a discriminant analysis, four variables were selected that explained the variation between Brazilian states that were “free” and “not free” of FMD while another four were selected to explain the variation between the zones “approved” and “not approved” to export meat to the EU, including number of official veterinarians, total transit of bovines and buffaloes, total number of animal transit certificates issued for bovine and buffaloes at the state or zone level, and total number of municipalities in the state or zone. It was possible to correctly discriminate between “free” and “not free” FMD states or zones. Variables related to animal transit are important in assessing the state for the classification of animal health situation and for EU approval for the exportation of meat.  相似文献   

Prevalence of antimicrobial resistance in enteric Escherichia coli from domestic pets and assessment of associated risk markers using a generalized linear mixed model     

Liliana R. Leite-Martins  Maria I.M. Mahú  Ana L. Costa  Ângelo Mendes  Elisabete Lopes  Denisa M.V. Mendonça  João J.R. Niza-Ribeiro  Augusto J.F. de Matos  Paulo Martins da Costa 《Preventive veterinary medicine》2014

Antimicrobial resistance (AMR) is a growing global public health problem, which is caused by the use of antimicrobials in both human and animal medical practice. The objectives of the present cross-sectional study were as follows: (1) to determine the prevalence of resistance in Escherichia coli isolated from the feces of pets from the Porto region of Portugal against 19 antimicrobial agents and (2) to assess the individual, clinical and environmental characteristics associated with each pet as risk markers for the AMR of the E. coli isolates.  相似文献   

Molecular characterization of canine coronavirus strains circulating in Brazil   总被引：1，自引：0，他引：1  

Erika Moutinho Costa  Tatiana Xavier de CastroFernanda de Oliveira Bottino  Rita de Cássia Nasser Cubel Garcia 《Veterinary microbiology》2014

To characterize canine coronavirus (CCoV) circulating in diarrheic puppies in Brazil, 250 fecal samples collected between 2006 and 2012 were tested. By using RT-PCR to partially amplify the M gene, CCoV RNA was detected in 30 samples. Sequence analysis of the M protein grouped eight strains with CCoV-I and another 19 with CCoV-II prototypes. To genotype/subtype the CCoV strains and assess the occurrence of single or multiple CCoV infections, RT-PCR of the S gene was performed, and 25/30 CCoV-positive strains amplified with one or two primer pairs. For 17/25 samples, single infections were detected as follows: six CCoV-I, nine CCoV-IIa and two CCoV-IIb. Eight samples were positive for more than one genotype/subtype as follows: seven CCoV-I/IIa and one CCoV-I/IIb. Sequence analysis revealed that the CCoV-I and IIa strains shared high genetic similarity to each other and to the prototypes. The Brazilian strains of CCoV-IIb displayed an aminoacid insertion that was also described in CCoV-IIb-UCD-1 and TGEV strains. Among the 25 CCoV-positive puppies, five had a fatal outcome, all but one of which were cases of mixed infection. The current study is the first reported molecular characterization of CCoV-I, IIa and IIb strains in Brazil.  相似文献   

Use of Thermography Techniques in Equines: Principles and Applications     

Veronica Redaelli  Domenico Bergero  Enrica Zucca  Francesco Ferrucci  Leonardo Nanni Costa  Lorenzo Crosta  Fabio Luzi 《Journal of Equine Veterinary Science》2014

This review of the use of thermographic technique in equines introduces the principles upon which infrared radiation and thermoregulatory physiology are based and describes the instrumentation used and its practical use. The advantage of this imaging technique is that it is a noninvasive thermographic examination, both from an operational (the animal and the operator) and health (no penetrating radiation is used) standpoint. Advantages and disadvantages of this technique, equine applications, and physiological assessments are discussed.  相似文献   

Ultrastructural Morphology and Nuclear Maturation Rates of Immature Equine Oocytes Vitrified with Different Solutions and Exposure Times     

Bruna R. Curcio  Melba O. Gastal  Gabriel R. Pereira  Carine D. Corcini  Fernanda C. Landim-Alvarenga  Severo S. Barros  Carlos E.W. Nogueira  João Carlos Deschamps  Eduardo L. Gastal 《Journal of Equine Veterinary Science》2014

Ultrastructural morphological injuries and maturation rates were investigated in equine oocytes exposed to vitrification solutions (VS) containing synthetic ice blockers (SIBs) during different exposure times. In experiment 1, compact cumulus-oocyte complexes (COCs; n = 30) were randomly allocated to treatments: (1) fresh fixed (control); (2) VS-1 (1.4 M dimethyl sulfoxide [DMSO] + 1.8 M ethylene glycol [EG] + 1% SIB) for 3 minutes of equilibrium time and VS-2 (2.8 M DMSO + 3.6 M EG + 0.6 M sucrose + 1% SIB) for 1 minute (Eq-long); and (3) VS-1 for 1.5 minutes and VS-2 for 30 seconds (Eq-short). In experiment 2, compact (n = 248) and expanded (n = 264) COCs were evenly distributed to the following treatments: (1) immediate maturation in vitro (control); (2) vitrification using the Eq-short protocol as in experiment 1; and (3) vitrification using a stock solution containing 2.8 M formamide, 2.8 M DMSO, 2.7 M EG, 7% polyvinylpyrrolidone, and 1% SIB (Eq-short-mod). More (P < .02) oocytes with normal ultrastructural morphology were seen in fresh control and Eq-short groups than in Eq-long group. Metaphase-II (MII) rates were higher (P < .05) for oocytes with expanded cumulus than compact cumulus in the control group, and higher (P < .05) for oocytes with expanded cumulus than compact cumulus in Eq-short and Eq-short-mod groups. No difference in MII rates was detected among groups within each type of COC. In conclusion, reduction of exposure time to VS better preserved oocyte ultrastructural features, and MII rates were higher for vitrified oocytes with expanded cumulus. This study advances our knowledge on potential alternatives for vitrification of immature equine oocytes.  相似文献   

Follicular dynamics in mules     

E.L. Gastal  L.O. Barros  G.F. Carneiro  M.O. Gastal 《Journal of Equine Veterinary Science》2014

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Effect of Breeding Activity on the Microflora of the External Genitalia and in the Semen of Stallions,and the Relationship Between Micro‐organisms on the Skin and on the External Genitalia     

T Guimarães  C Miranda  M Pinto  E Silva  L Damásio  AL Costa  MJ Correia  JC Duarte  C Cosinha  G Lopes  G Thompson  A Rocha 《Reproduction in domestic animals》2014,49(6):926-933

A possible role of breeding activities in the composition of the microbial population in stallions' external genitalia (EG) and the relationship between micro‐organisms colonizing the skin of the abdomen and the ones colonizing the EG have not been studied. In experiment 1, EG microbiological samples were collected from 41 stallions used for both natural cover and semen collection (BST) and from 18 non‐breeding stallions (NBST). A higher (p < 0.05) frequency of isolation of potentially pathogenic species was found for BST. Age did not influence number of micro‐organism species isolated both in BST and NBST. In experiment 2, the microbial content of the EG and semen was compared in 23 BST. Most micro‐organisms isolated from the EG were present in semen, albeit with a numerically lower prevalence. In 7 stallions, six microbial species isolated from semen were absent from the EG cultures, suggesting contamination by the operator. In experiment 3, a numerically higher number of micro‐organism species was isolated from the EG of 31 stallions, than from their skin of the ventral abdomen in contact with the penis or from the skin of the thorax. With the sole exception of Escherichia coli, potentially pathogenic bacteria were only isolated from the EG but not from the skin. Results suggest that breeding activity increased the number of species colonizing the EG; most species isolated from the EG were also found in semen even if with a lower frequency, and additional semen contamination seemed to occur during its manipulation. Many micro‐organism species of the skin were also isolated from the penis, but independently of being or not in contact with the penis, skin did not seem to provide an adequate environment for the growth of potentially pathogenic bacteria that were isolated from EG, with the sole exception for E. coli.  相似文献   

Occurrence and present status of coastal dolphins (Delphinus delphis and Tursiops truncatus) in the eastern Ionian Sea     

Giovanni Bearzi  Elena Politi  Stefano Agazzi  Sebastiano Bruno  Marina Costa  Silvia Bonizzoni 《水产资源保护:海洋与淡水生态系统》2005,15(3):243-257

• 1. Boat surveys aimed at studying short‐beaked common dolphins and common bottlenose dolphins in eastern Ionian Sea coastal waters were conducted between 1993 and 2003. During 835 survey days, 24 771 km of total effort was distributed within an area of 480 km², resulting in 428 common dolphin and 235 bottlenose dolphin sightings.
• 2. Individual photo‐identification was performed extensively throughout this study, making it possible to monitor the number of animals seen in the study area each year and their long‐term residency patterns.
• 3. Common dolphins declined across the study period, from 2.18 encounters/100 km in 1997 to 0.40 encounters/100 km in 2003. In contrast, there was a relatively stable presence of bottlenose dolphins, some individuals showing high levels of site fidelity and others using the area only occasionally.
• 4. The local decline of common dolphins and the low density of bottlenose dolphins appeared to reflect the general status of these cetacean species in the wider Mediterranean region, where common dolphins were classified as endangered in the IUCN Red List in 2003.
• 5. Based on the available evidence, we infer that the present unfavourable status of common dolphins in eastern Ionian Sea coastal waters is largely a consequence of prey depletion.

Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

A comparative study of corneal sensitivity in birds of prey     

Rodrigo P. Lacerda  Elena Obón  Maria T. Peña  Daniel Costa  Jose Ríos  Marta Leiva 《Veterinary ophthalmology》2014,17(3):190-194

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Dissolution Factors of Ta, Th, and U Oxides Present in Pyrochlore     

K. Dias da Cunha  M. Santos  F. Zouain  L. Carneiro  G. Pitassi  C. Lima  C. V. Barros Leite  K. C. P. Dália 《Water, air, and soil pollution》2010,205(1-4):251-257

Air pollution can be a problem in industrial processes, but monitoring and controling the aerosols in the work place is not enough to estimate the occupational risk due to dust particle inhalation. The solubility in lung fluid is considered to estimate this risk. The aim of this study is to determine in vitro specific dissolution parameters for thorium (Th), uranium (U), and tantalum (Ta) associated to crystal lattice of a niobium mineral (pyrochlore). Th, U, and Ta dissolution factors in vitro were obtained using the Gamble solution (simulant lung fluid, SLF), particle induced X-ray emission, and alpha spectrometry as analytical techniques. Ta, Th, and U are present in the pyrochlore crystal lattice as oxide; however, they have shown different dissolution parameters. The rapid dissolution fraction (f _r), rapid dissolution rate (λ _r), slow dissolution rate (f _s), and slow dissolution fraction (λ _s) measured for tantalum oxide were equal to 0.1 and 0.45 and 0.00007 day^?1, respectively. For uranium oxide, f _r was equal to 0.05, λ _r was equal to 1.1 day^?1, and λ _s was equal to 0.000068 day^?1. For thorium oxide, f _r was 0.025, λ _r was 1.5 day^?1, and λ _s was 0.000065 day^?1. These results show that chemical behavior of these three compounds in the SLF could not be represented by the same parameter. The ratio of uranium concentration in urine and feces samples from workers exposed to pyrochlore dust particle was determined. These values agree with the theoretical values of estimated uranium concentration using specific parameters for uranium oxide present in pyrochlore.  相似文献