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11.
Effects of road transportation or droving on the weight and metabolism of young bulls 总被引：1，自引：0，他引：1
Marcella Cândia D’Oliveira Maria Inês Lenz Souza Ruy Alberto Caetano Corrêa Filho Maria da Graça Morais Camila Celeste Brandão Ferreira Ítavo Gumercindo Loriano Franco 《Tropical animal health and production》2014,46(8):1447-1453
This study evaluated the effects of the mode of transportation on weight and metabolism of young bulls, which are generally transported by two systems: droving and truck. This experiment consisted of two phases: transportation and refeeding and involved transportation by droving and truck. Fifty young Nellore bulls were separated in equal numbers in both groups, droving group and truck group. In the transportation phase, animals were moved, in a 640-km journey. The refeeding phase consisted of 84 days and commenced after the animals arrived at the fattening farm. The effect of the transportation system on animals’ weight, metabolic hormones, and serum biochemistry was assessed after 28, 56, and 84 days. During the transportation phase, animals in the droving group lost 10.11 kg and animals in the truck group gained 13.1 kg. The truck group showed 20.5 kg of weight gain at the end of the refeeding phase. The highest triiodothyronine (T3) and thyroxine (T4) concentrations were observed on days 0 and 60 in the transportation phase in both droving and truck groups. The serum cortisol concentration was higher in the droving group than in the truck group on day 56 of the refeeding phase. The IGF-I concentrations were higher during the refeeding phase in the droving group than in the truck group. Our results showed that transportation by droving leads to increased weight loss and reduced further weight gain compared to transportation by truck and that serum concentrations of T4, cortisol, and insulin-like growth factor (IGF-I) hormones are altered by the transportation system. 相似文献
12.
Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5 总被引：1，自引：0，他引：1
Fischer G Cleff MB Dummer LA Paulino N Paulino AS de Oliveira Vilela C Campos FS Storch T D'Avila Vargas G de Oliveira Hübner S Vidor T 《Veterinary immunology and immunopathology》2007,116(1-2):79-84
Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines. 相似文献
13.
Bonnefont-Rebeix C de Carvalho CM Bernaud J Chabanne L Marchal T Rigal D 《Veterinary immunology and immunopathology》2006,109(1-2):167-176
In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC. 相似文献
14.
Leptospirosis as the most frequent infectious disease impairing productivity in small ruminants in Rio de Janeiro,Brazil 总被引：2，自引：2，他引：0
Martins G Penna B Hamond C Leite RC Silva A Ferreira A Brandão F Oliveira F Lilenbaum W 《Tropical animal health and production》2012,44(4):773-777
Despite the importance of small ruminants breeding in developing countries, milk/meat productivity remains unsatisfactory.
Infectious diseases, such as leptospirosis, brucellosis, and small ruminant lentiviruses (SRLVs), contribute to this scenario.
The objective of the present study was to determine the role of each of these diseases in the productivity of small ruminants
breeding in Rio de Janeiro, Brazil. In goats, 343 samples were tested for leptospirosis, 560 for Brucella abortus, and 506 for caprine arthritis-encephalitis (CAE), whereas in sheep, 308 samples were tested for leptospirosis, 319 for B. abortus, 374 for Brucella ovis, and 278 for Maedi-Visna (MV). Regarding leptospirosis, 25.9% of goats and 47.4% sheep were seroreactive, with serovar Hardjo
the most prevalent in both species. Anti-B. abortus agglutinins were found in 0.7% of all samples, exclusively in goats. In relation to SRLVs, 8.6% of goats and 3.2% of sheep
samples were positive for CAE and MV, respectively. Leptospirosis was the major infectious problem in the small ruminants
sampled and may contribute to impaired productivity of these animals. 相似文献
15.
Gelaleti GB Jardim BV Leonel C Moschetta MG Zuccari DA 《Veterinary immunology and immunopathology》2012,146(2):106-112
Mammary gland tumors in female dogs are an excellent model for the clinic-pathological, diagnostic and prognostic investigation of mammary neoplasias. Prognostic and predictive markers are effective in research and routine diagnosis. Interleukins play a fundamental role in cancer, with a particular function in tumor growth, invasion and metastatic potential. Interleukin-8 (IL-8) is known to possess tumorigenic and pro-angiogenic properties, and its overexpression is seen in a number of human tumors. IL-8 serum levels were determined and correlated with the clinic-pathological features and clinical evolution of mammary gland neoplasias in female dogs. IL-8 was measured by an immunoenzymatic assay in 30 female dogs with mammary neoplasias within a 12 month follow-up and in 50 control animals. The correlation between IL-8 concentration and clinical parameters was investigated. A statistically significant difference in the IL-8 serum levels was found in tumor-bearing dogs compared to the controls. In addition, when the individual parameters were evaluated, IL-8 content showed a positive correlation with the tumor progression, lymph node involvement, recurrence and death. Single and multivariate analyses showed associations between tumor recurrence, metastasis, high clinical staging and high IL-8, and also with the death risk. This was also consistent with the high IL-8 content in dogs showing tumor recurrence and metastasis. IL-8 superexpression has been detected in a number of human tumors, usually associated with a poor prognostic. Besides promoting angiogenesis, IL-8 is strongly related with the metastatic phenotype of mammary tumor cells. High IL-8 concentration was found in mammary gland cancer patients with advanced disease stages. Our results show that IL-8 can be used as a non-invasive prognostic marker for mammary gland cancer, and can be useful for the prediction of disease progression and recurrence in dogs with mammary neoplasias. The increased level of this cytokine acts as an independent prognostic marker of survival and the identification of animals with the poor prognostic. 相似文献
16.
Camila Hamond Gabriel Martins Marco A. Medeiros Walter Lilenbaum 《Journal of Equine Veterinary Science》2013
The purpose of the present study was to detect leptospiral DNA by PCR in semen and urine samples of stallions to test for venereal transmission in horses. A total of 10 stallions from four herds were studied, and sampling was conducted in semen and urine for culture and PCR and serum for serology. From the 10 serum samples tested, 6 (60%) were seroreactive. No pure culture was obtained, but leptospiral DNA was detected by PCR in 50% of the semen samples and 30% of urine samples. The present study aimed to detect leptospiral DNA by PCR in semen and urine samples of stallions to test for venereal transmission in horses. Based on these findings, we suggest that there is potential transmission of leptospirosis in horses by sexual transmission. 相似文献
17.
do Amaral Elizabeth Santos Vieira Silva Daniela Dos Anjos Letícia Schilling Ana Cristina Dalmolin Ândrea Carla Mielke Marcelo Schramm 《New Forests》2019,50(3):377-388
Methods based on RGB (Red, Green, Blue) image segmentation may emerge as a new and low-cost method for estimation the quality of tree seedlings. However, the vast number of indexes based on the use of the RGB image segmentation and the lack of references in the literature still hinder the widespread use of this technology. Thus, we conducted a study aiming to test the relationships between methods based on absorbance and reflectance, widely used for the estimation of chlorophyll contents and physiological status of trees, and ten indexes based on RGB component analysis. We used leaves of five tropical tree species, belonging to different botanical families. Leaf absorbance was measured using the handheld chlorophyll meter SPAD-502, reflectance was measured using a spectrometer and the RGB indices were obtained from digitalized images of the leaves using a flatbed scanner. Modified linear regression models including all five species were used to relate RGB indices to absorbance and reflectance indices. Data collected from leaves of seedlings of five tropical tree species indicated that digital image processing technology can be a useful and rapid nondestructive method for assessment of physiological status of tree seedlings at nursery stage. Among the RGB indexes tested in this study the R, 2R*(G???B)/(G?+?B) and 2G*(G???B)/(G?+?B) are the most promising for analysis the tropical seedlings physiological status and quality.
相似文献18.
Hugo Vilhena Asta Tvarijonaviciute Jos J. Cern Ana C. Figueira Snia Miranda Ana Ribeiro Ana Canadas Patrícia Dias‐Pereira Camila P. Rubio Lorena Franco Fernando Tecles Ricardo Cabeas Josep Pastor Ana C. Silvestre‐Ferreira 《Veterinary and comparative oncology》2019,17(3):394-406
Acute phase proteins (APP) and biomarkers of oxidative status change in human and canine mammary tumours, however, they have not been studied in feline mammary tumours. The aims of this study were to investigate the APP and antioxidant responses in feline malignant mammary tumours, to evaluate their relation with tumour features, and to assess their prognostic value. Serum amyloid A (SAA), haptoglobin (Hp), albumin, butyrylcholinesterase (BChE), insulin‐like growth factor1 (IGF1), paraoxonase1 (PON1), total serum thiols (Thiol), glutathione peroxidase (GPox) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity assessed by two different methodologies (TEAC1/2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in serum of 50 queens with spontaneous mammary carcinomas and of 12 healthy female cats. At diagnosis, diseased queens presented significantly higher SAA and Hp, and lower albumin, BChE, GPox, TEAC1, TEAC2 and CUPRAC than controls. Different tumour features influenced concentrations of APP and antioxidants. Increases in serum Hp, and decreases in albumin, Thiol and FRAP were significantly associated with neoplastic vascular emboli, metastasis in regional lymph nodes and/or in distant organs. Distant metastasis development during the course of the disease was associated with increases in SAA and TEAC1. At diagnosis, decreased albumin was associated with a longer survival, and BChE <1.15 μmoL/mL.minute was associated with a shorter survival time on multivariate analysis. Feline malignant mammary tumours are associated with an APP response and oxidative stress, and different tumour features influence the inflammatory response and the oxidative damage. Furthermore, some of these analytes proved to have prognostic value. 相似文献
19.
Pimenta Carla Lauise Rodrigues Menezes da Costa Diego Figueiredo Silva Maria Luana Cristiny Rodrigues Pereira Hélio Domingos Júnior João Pessoa Araújo Malossi Camila Dantas Ullmann Leila Sabrina Alves Clebert José de Azevedo Sérgio Santos 《Tropical animal health and production》2019,51(1):237-241
The aim of the present study was to describe the strategies of the control of an outbreak of leptospiral infection in dairy cattle in Maranhão State, Northeastern Brazil. In the period from January to July 2015, 18 (17%) out of 106 cows presented abortion, six (5.7%) stillbirth, and 12 (11.3%) repeated estrus, totaling 24 animals with reproductive problems. The diagnosis of leptospirosis was based on serology (microscopic agglutination test—MAT), bacteriological culture, and polymerase chain reaction (PCR). Antibiotic therapy, vaccination protocols, and changes in management practices were suggested as control measures. Of all animals on the farm (n?=?280), 136 (48.6%) were seropositive for at least one serovar of Leptospira sp. No pure leptospiral culture was obtained. Eight of the animals with reproductive problems yielded positive PCR results (vaginal fluid of seven animals and urine and vaginal fluid of one animal). Genetic sequencing of a vaginal fluid/urine PCR-positive sample revealed Leptospira borgpetersenii. One year after the adoption of control measures, no reproductive problems were observed. Thus, leptospirosis probably caused the reproductive failures in the herd, and the control and prevention measures implemented were efficient in controlling the disease.
相似文献20.
Tatiene R. M. Silva Flvia N. L. Barros Michele Bahia Francisco D. Sampaio Junior Sidney S. F. Santos Larissa S. Inoue Thamirys S. Gonalves Laerzio Chiesorin Neto Diogo C. L. O. Faria Camila Tochetto Gisele M. R. Viana Frederico O. B. Monteiro Gustavo Ges-Cavalcante Alessandra Scofield 《Zoonoses and public health》2019,66(7):798-804
The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free‐born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi‐nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi‐nested PCR. The presence of infected non‐human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species. 相似文献