Diversity,occurrence, and life characteristics of natural entomopathogenic nematode populations from La Rioja (Northern Spain) under different agricultural management and their relationships with soil factors     

Raquel Campos-Herrera  José Manuel Gómez-Ros  Miguel Escuer  Luis Cuadra  Laura Barrios  Carmen Gutiérrez 《Soil biology & biochemistry》2008,40(6):1474-1484

Natural entomopathogenic nematode (EPN) populations were surveyed in La Rioja (Northern Spain) during two consecutive years (2003–2005) to study their diversity, occurrence, and life characteristics under different agricultural management representing natural areas and perennial and annual crops from organic and conventional systems. Native EPN species and strains were identified using morphological and molecular characteristics. Virulence, infection cycle length and reproductive potential were assessed using Galleria mellonella larvae. The EPN occurrence was evaluated through abundance, recovery frequency, larval mortality percentage and EPN population density. EPNs were also related to selected soil physical and chemical variables as well as to some soil pollutants such as heavy metals and organochlorine pesticide residues. Only two steinernematids species were identified: Steinernema feltiae was observed throughout all seasons from natural and agricultural areas and Steinernema carpocapsae in summer and autumn of 2004 from perennial crops only. The virulence of native strains was lower than other previously isolated Spanish strain from natural areas or crop field edges. EPN abundance and recovery frequency indicated that habitat type might influence EPNs occurrence stronger than seasonality with the intensity of agricultural management inversely affecting their distribution. Moreover, clay, P₂O₅, Zn, Cu and hexachlorobenzene contents negatively correlating with EPN population density. We consider that agricultural management should be taken into account if EPNs are going to be used as biological control agents.  相似文献   

Potential biomedical reuse of vegetative residuals from mycorrhized grapevines subjected to warming     

Nazareth Torres  Daniel Plano  M. Carmen Antolín  Carmen Sanmartín  Maite Domínguez-Fernández  María-Paz De Peña 《Archives of Agronomy and Soil Science》2019,65(10):1341-1353

Grapevine leaves are widely discarded in open fields despite their known antioxidant properties. We tested the cytotoxicity of leaf extracts from three clones (CL-260, CL-1048, CL-8) of Vitis vinifera L. cv. Tempranillo against four human cancer cell lines: colon, HT-29; breast, MCF-7; lung HTB-54; and lymphoblastic leukemia, CCRF-CEM. Grapevines were cultivated at either ambient (24/14 °C) or elevated (28/18 °C) day/night temperatures, and inoculated (+M) or not (-M) with arbuscular mycorrhizal fungi (AMF). Cytotoxicity was analysed by MTT assays. Elevated air temperatures enhanced the cytotoxicity of leaf extracts from CL-260 against HT-29, CCRF-CEM and HTB-54 and that from CL-8 against MCF-7. Mycorrhization improved the cytotoxicity of leaf extracts from CL-1048 against HT-29, CCRF-CEM, HTB-54 and MCF-7. The cytotoxic activities of CL-260 against HTB-54 and CL-1048 against HT-29 were correlated, respectively, with total phenols and total antioxidant capacity. We conclude that the predicted increase in air temperature for the future climate and the mycorrhizal association of grapevines may enhance the cytotoxicity of leaves, which strengthens the potential application of these agricultural residuals for biomedicine. However, the clonal diversity in the response to AMF and air temperature highlights the importance of choosing the most adequate clone for a concrete environmental scenario.  相似文献   

Genetic and geographic variation in growth of Balanites aegyptiaca in Niger: comparing results from provenance/progeny tests in the nursery and field     

Weber  John C.  Sotelo Montes  Carmen  Soumana  Idrissa  Diallo  Boukary Ousmane  Abasse  Tougiani  Larwanou  Mahamane  Bationo  André Babou 《New Forests》2019,50(4):643-661

New Forests - Some tree improvement programs in Africa use nursery tests to investigate genetic and geographic variation in growth, but do they lead to the same conclusions as field tests? We...  相似文献   

Metal mobility and mineral transformations driven by bacterial activity in acidic pit lake sediments: evidence from column experiments and sequential extraction     

Diez-Ercilla  Marta  Falagán  Carmen  Yusta  Iñaki  Sánchez-España  Javier 《Journal of Soils and Sediments》2019,19(3):1527-1542

Journal of Soils and Sediments - Biogeochemical processes occurring in lake sediments are crucial for element cycling. In acidic pit lakes, they play a major role in acidity neutralization and...  相似文献   

Compost-assisted phytoremediation of As-polluted soil     

González  Águeda  García-Gonzalo  Pilar  Gil-Díaz  M. Mar  Alonso  Juan  Lobo  M. Carmen 《Journal of Soils and Sediments》2019,19(7):2971-2983

Journal of Soils and Sediments - The effect of organic matter on the As bioavailability in soils and thus on phytoremediation processes is still controversial. The objective of the present study...  相似文献   

Nutritional assessment of charitable meal programmes serving homeless people in Toronto     

Tse C  Tarasuk V 《Public health nutrition》2008,11(12):1296-1305

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Mapping within-field variability in wheat yield and biomass using remote sensing vegetation indices     

Campos  Isidro  González-Gómez  Laura  Villodre  Julio  Calera  Maria  Campoy  Jaime  Jiménez  Nuria  Plaza  Carmen  Sánchez-Prieto  Sergio  Calera  Alfonso 《Precision Agriculture》2019,20(2):214-236

Precision Agriculture - This paper explored the ability of remote sensing (RS) and meteorological data to map the variability of yield/biomass in cultivated wheat (Triticum aestivum). The...  相似文献   

Control of Fusarium verticillioides,cause of ear rot of maize,by Pseudomonas fluorescens     

Siddaiah Chandra Nayaka  Arakere C Udaya Shankar  Munagala S Reddy  Siddapura R Niranjana  Harishchandra S Prakash  Hunthrike S Shetty  Carmen N Mortensen 《Pest management science》2009,65(7):769-775

BACKGROUND: Maize is one of the staple food crops grown in India. Fusarium verticillioides (Sacc.) Nirenberg is the most important fungal pathogen of maize, associated with diseases such as ear rot and kernel rot. Apart from the disease, it is capable of producing fumonisins, which have elicited considerable attention over the past decade owing to their association with animal disease syndromes. Hence, the present study was conducted to evaluate ecofriendly approaches by using a maize rhizosphere isolate of Pseudomonas fluorescens (Trev.) Mig. and its formulation to control ear rot disease and fumonisin accumulation, and also to study the capacity to promote growth and yield of maize. In vitro assays were conducted to test the efficacy of P. fluorescens as a seed treatment on seed germination, seedling vigour and also the incidence of F. verticillioides in different maize cultivars. The field trials included both seed treatment and foliar spray. For all the experiments, P. fluorescens was formulated using corn starch, wheat bran and talc powder. In each case there were three different treatments of P. fluorescens, a non‐treated control and chemical control. RESULTS: Pure culture and the formulations, in comparison with the control, increased plant growth and vigour as measured by seed germination, seedling vigour, plant height, 1000 seed weight and yield. P. fluorescens pure culture used as seed treatment and as spray treatment enhanced the growth parameters and reduced the incidence of F. verticillioides and the level of fumonisins to a maximum extent compared with the other treatments. CONCLUSION: The study demonstrates the potential role of P. fluorescens and its formulations in ear rot disease management. The biocontrol potential of this isolate is more suited for fumonisin reduction in maize kernels intended for human and animal feed. Copyright © 2009 Society of Chemical Industry  相似文献   

Effect of Protein Hydrolysates from Germinated Soybean on Cancerous Cells of the Human Cervix: An <Emphasis Type="Italic">In Vitro</Emphasis> Study     

R. Mora-Escobedo  Maria del Carmen Robles-Ramírez  Eva Ramón-Gallegos  Rafael Reza-Alemán 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):271-278

Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of amino acids or bioactive peptides. Soybean was germinated for 0, 2, 3, 4, 5, and 6 days and proteins were isolated from the seeds. Isolates with and without ethanol-soluble phytochemicals were hydrolyzed with digestive enzymes and their effect on growth in HeLa and C-33 (epidermoid cervical carcinoma) and HaCaT (non-cancerous human keratinocytes) cells were evaluated with the Alamar Blue method. Germination induced degradation of the α and α’ fractions of β-conglycinin and acid fraction of glycinin, generating low molecular weight peptides. Degrees of hydrolysis ranged from 73–77%. Hydrolysates inhibited the growth of HeLa cells and C-33 at concentrations exceeding 1.25 mg/ml. Major inhibition was observed with the hydrolysate germinated for 2 days and containing ethanolsoluble phytochemicals (IC₅₀ 2.15 and 2.27 mg/ml for HeLa and C-33, respectively). Interestingly, hydrolysate cytoxicity for normal cells was minimal in comparison to cancer cells.  相似文献   

Nonfatal infusion pump-related venous air embolism in a dog     

Cristina Costa-Farré  Carlos Torrente  Carmen Diaz Bertrana  Ignacio A. Gomez de Segura 《Veterinary anaesthesia and analgesia》2017,44(2):382-383

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