The V-antigen of Yersinia forms a distinct structure at the tip of injectisome needles     

Mueller CA  Broz P  Müller SA  Ringler P  Erne-Brand F  Sorg I  Kuhn M  Engel A  Cornelis GR 《Science (New York, N.Y.)》2005,310(5748):674-676

Many pathogenic bacteria use injectisomes to deliver effector proteins into host cells through type III secretion. Injectisomes consist of a basal body embedded in the bacterial membranes and a needle. In Yersinia, translocation of effectors requires the YopB and YopD proteins, which form a pore in the target cell membrane, and the LcrV protein, which assists the assembly of the pore. Here we report that LcrV forms a distinct structure at the tip of the needle, the tip complex. This unique localization of LcrV may explain its crucial role in the translocation process and its efficacy as the main protective antigen against plague.  相似文献   

Medicine. Targeting apoptotic pathways in cancer cells     

Denicourt C  Dowdy SF 《Science (New York, N.Y.)》2004,305(5689):1411-1413

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Polymerizing actin fibers position integrins primed to probe for adhesion sites     

Galbraith CG  Yamada KM  Galbraith JA 《Science (New York, N.Y.)》2007,315(5814):992-995

Migrating cells extend protrusions, probing the surrounding matrix in search of permissive sites to form adhesions. We found that actin fibers polymerizing along the leading edge directed local protrusions and drove synchronous sideways movement of beta1 integrin adhesion receptors. These movements lead to the clustering and positioning of conformationally activated, but unligated, beta1 integrins along the leading edge of fibroblast lamellae and growth cone filopodia. Thus, rapid actin-based movement of primed integrins along the leading edge suggests a "sticky fingers" mechanism to probe for new adhesion sites and to direct migration.  相似文献   

When goliaths clash: US and EU differences over the labeling of food products derived from genetically modified organisms     

Andy Thorpe  Catherine Robinson 《Agriculture and Human Values》2004,21(4):287-298

There is a fundamental divergence of opinion between the EU and the US over how food products derived from genetically modified organisms should be labeled. This has less to do with safety, as moves towards the international harmonization of safety standards continue apace, and rather more to do with the consumers' right to know about the origins of the food they are consuming. This paper uses a framework drawn from the global public goods (GPG) literature of economics and the work by international relations theorists on formal international organizations (FIO) to explain why there is presently no global consensus on the manner (voluntary or mandatory) in which GM food products should be labeled.  相似文献   

A physical map of the 1-gigabase bread wheat chromosome 3B   总被引：2，自引：0，他引：2  

Paux E  Sourdille P  Salse J  Saintenac C  Choulet F  Leroy P  Korol A  Michalak M  Kianian S  Spielmeyer W  Lagudah E  Somers D  Kilian A  Alaux M  Vautrin S  Bergès H  Eversole K  Appels R  Safar J  Simkova H  Dolezel J  Bernard M  Feuillet C 《Science (New York, N.Y.)》2008,322(5898):101-104

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.  相似文献   

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A     

Cruz-Migoni A  Hautbergue GM  Artymiuk PJ  Baker PJ  Bokori-Brown M  Chang CT  Dickman MJ  Essex-Lopresti A  Harding SV  Mahadi NM  Marshall LE  Mobbs GW  Mohamed R  Nathan S  Ngugi SA  Ong C  Ooi WF  Partridge LJ  Phillips HL  Raih MF  Ruzheinikov S  Sarkar-Tyson M  Sedelnikova SE  Smither SJ  Tan P  Titball RW  Wilson SA  Rice DW 《Science (New York, N.Y.)》2011,334(6057):821-824

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.  相似文献   

A synthetic maternal-effect selfish genetic element drives population replacement in Drosophila     

Chen CH  Huang H  Ward CM  Su JT  Schaeffer LV  Guo M  Hay BA 《Science (New York, N.Y.)》2007,316(5824):597-600

One proposed strategy for controlling the transmission of insect-borne pathogens uses a drive mechanism to ensure the rapid spread of transgenes conferring disease refractoriness throughout wild populations. Here, we report the creation of maternal-effect selfish genetic elements in Drosophila that drive population replacement and are resistant to recombination-mediated dissociation of drive and disease refractoriness functions. These selfish elements use microRNA-mediated silencing of a maternally expressed gene essential for embryogenesis, which is coupled with early zygotic expression of a rescuing transgene.  相似文献   

Fragmentation and flow regulation of the world's large river systems   总被引：7，自引：0，他引：7  

Nilsson C  Reidy CA  Dynesius M  Revenga C 《Science (New York, N.Y.)》2005,308(5720):405-408

A global overview of dam-based impacts on large river systems shows that over half (172 out of 292) are affected by dams, including the eight most biogeographically diverse. Dam-impacted catchments experience higher irrigation pressure and about 25 times more economic activity per unit of water than do unaffected catchments. In view of projected changes in climate and water resource use, these findings can be used to identify ecological risks associated with further impacts on large river systems.  相似文献   

Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet     

Seo CW  Um IC  Rico CW  Kang MY 《Journal of agricultural and food chemistry》2011,59(8):4192-4197

The effect of silk protein with different fibroin/sericin compositions on body weight and lipid metabolism in high fat-fed mice was investigated. The animals were given experimental diets for 6 weeks: normal control (NC), high fat (HF) and high fat diet supplemented with F100 (pure fibroin, HF-F100), F81 (81:19 fibroin/sericin, w/w, HF-F81) or F50 (50:50 fibroin/sericin, w/w, HF-F50). The silk protein-fed mice showed markedly reduced body weight and enhanced lipid profile relative to the HF group. In general, the amount of body fat, triglyceride and total plasma cholesterol levels, atherogenic index and free fatty acid level tended to decrease, while the HDL-cholesterol level increased, with increased amount of sericin in the diet. This hypolipidemic effect was partly due to increased fecal lipid excretion, inhibition of lipogenesis and regulation of adipokine production. These findings illustrate that silk protein, particularly sericin, may be beneficial in the prevention of high fat diet-induced hyperlipidemia and obesity.  相似文献   

Competencies: a cure for pre-med curriculum     

Anderson WA  Amasino RM  Ares M  Banerjee U  Bartel B  Corces VG  Drennan CL  Elgin SC  Epstein IR  Fanning E  Guillette LJ  Handelsman J  Hatfull GF  Hoy RR  Kelley D  Leinwand LA  Losick R  Lu Y  Lynn DG  Neuhauser C  O'Dowd DK  Olivera T  Pevzner P  Richards-Kortum RR  Rine J  Sah RL  Strobel SA  Walker GC  Walt DR  Warner IM  Wessler S  Willard HF  Zare RN 《Science (New York, N.Y.)》2011,334(6057):760-761

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