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81.
By increasing resource availability, canopy opening enhances tree recruitment as well as the development of neighbouring vegetation. The proliferation of early successional and highly competitive vegetation may have dramatic consequences on seedling establishment. However, differences in competitive abilities have been shown among the plant growth forms commonly encountered in forests. We may thus expect that vegetation management leading to control of different plant growth forms would have different consequences on tree seedling growth and development. To test this hypothesis, we analysed the effects of an intensity gradient of four vegetation control treatments (untreated, coppice control, coppice and non-tree plant control, and coppice, non-tree and pioneer (tree) plant control) on plant species richness and natural tree regeneration in three post-storm sites. Higher plant species richness and a better natural tree regeneration were observed in the more intensive treatments that significantly improved the balance of the relative abundance of tree species. Suppressing the more competitive vegetation, mainly Rubus fruticosus and graminoids, led to the recruitment and growth of tree species sensitive to competition and good tree species diversity. Practical recommendations in terms of vegetation control relative to tree regeneration and plant species richness are given.  相似文献   
82.
False thrombocytopenia may result from platelet aggregation, especially in feline ethylenediamine tetra-acetic acid (EDTA) blood specimens. Citrate, theophylline, adenosine and dipyridamole (CTAD) was added to 46 feline EDTA specimens to test its anti-aggregation action. Platelet aggregation was estimated from blood films and a complete blood count was performed with a Sysmex XT-2000iV analyser. Platelet aggregation score was >2 in 11/46 EDTA tubes and only in one EDTA+CTAD specimen. The platelet count was higher in all CTAD-supplemented tubes except one, medians measured by cytometry being 225.5 × 10(9)/l and 249.0 × 10(9)/l in EDTA and EDTA+CTAD, respectively (P = 0.007). Adding CTAD had statistically and analytically significant but moderate effects on other blood variables, the most intense variations being observed for reticulocytes (about 3% higher in EDTA specimens) and reticulocyte indexes. Addition of CTAD to EDTA when sampling feline blood is a useful option to reduce platelet clumping.  相似文献   
83.
84.
The spatial pattern of apple scab was characterized using 10 years of disease incidence and lesion density data collected in managed orchards located in Quebec, Canada. Distributional analyses indicated that scab incidence was better characterized by the beta-binomial than the binomial distribution in 53 and 65% of the data sets at the leaf and shoot scales, respectively. Median values of the beta-binomial parameter θ, a measure of small-scale aggregation, were near 0 (0.003 and 0.028) at both sampling scales, indicating that disease incidence was close to being randomly distributed (low degree of aggregation). For lesion density, the negative binomial distribution fitted the data better than the Poisson distribution in 86% of the data sets at the leaf scale. The median value of the index of dispersion k was 0.068, indicating that aggregation was present. For all apple scab measurements, the power law models provided a good fit to the data. The estimated slope and intercept parameters were significantly greater than 1 and 0, respectively, suggesting that spatial heterogeneity changed systematically with disease incidence. Results of a covariance analysis showed that spatial heterogeneity of scab incidence at both scales and lesion density was not dependent upon shoot type but that spatial heterogeneity of scab incidence and lesion density at the leaf scale was influenced by the sampling period. A hierarchical analysis showed that scab incidence at the tree scale increased as a saturation-type curve with respect to incidence at the leaf or shoot scales. A similar relationship was observed for incidences at the shoot and leaf scales. An effective sample size model based on the binary power law parameters (Madden and Hughes, Phytopathology 89:770–781, 1999) gave the best fit to the leaf and shoot data, respectively. The incidence-lesion density relationship at both scales was well described by a complementary log-log (CLL) and log transformation model ( Radj2 = 0.97 and Radj2 = 0.94 ) \left( {R_{{adj}}^2 = 0.97\,and\,R_{{adj}}^2 = 0.94} \right) , however, the models tended to underestimate lesion density. The information of the spatial relations of apple scab within and between hierarchical scales acquired from this study can be used in developing and evaluating practical disease management strategies and to improve apple scab assessments for fungicide or cultivar susceptibility trials.  相似文献   
85.
The long-term (9 years) effect of pig slurry applications vs mineral fertilization on denitrifying activity, N2O production and soil organic carbon (C) (extractable C, microbial biomass C and total organic C) was compared at three soil depths of adjacent plots. The denitrifying activities were measured on undisturbed soil cores and on sieved soil samples with acetylene method to estimate denitrification rates under field or potential conditions. Pig slurry applications had a moderate impact on the C pools. Total organic C was increased by +6.5% and microbial biomass C by ≥25%. The potential denitrifying activity on soil suspension was stimulated (×1.8, P<0.05) 12 days after the last slurry application. This stimulation was still apparent, but not significant, 10 months later and, according to both methods of denitrifying activity measurement (r 2=0.916, P<0.01 on sieved soil; r 2=0.845, P<0.001 on soil cores), was associated with an increase in microbial biomass C above a threshold of about 105 mg kg−1. The effect of pig slurry on denitrification and N2O reduction rates was detected on the surface layer (0–20 cm) only. However, no pig slurry effect could be detected on soil cores at field conditions or after NO3 enrichments at 20°C. Although the potential denitrifying activity in sieved soil samples was stimulated, the N2O production was lower (P<0.03) in the plot fertilized with pig slurry, indicating a lower N2O/(N2O + N2) ratio of the released gases. The pig-slurry-fertilized plot also showed a higher N2O reduction activity, which is coherent with the lower N2O production in anaerobiosis.  相似文献   
86.
Flavonoids represent a large and important group of plant natural products that are ubiquitous in the plant kingdom. Epidemiological studies have shown the health benefits of a diet high in flavonoids. However, the dietary intake of flavonoids in most western populations is limited, creating a need to find alternative food sources for these polyphenolic secondary metabolites. The domestication of many of our cultivated food crops has resulted in alterations in the biosynthetic pathways of many essential micronutrients and vitamins through inadvertent counterselection against nutritional traits in favor of agronomic ones. Flavonoids are nearly absent from fruits of cultivated tomato (Lycopersicon esculentum Mill.), a major vegetable in human diets. Previous attempts to restore the flavonoid pathway in tomato fruits have been limited to transgenic strategies, suggesting that the problem was intractable through traditional methods. Here, we describe for the first time a nontransgenic metabolic engineering approach to developing a high flavonoid tomato using a wild tomato species (Lycopersicon pennelliiv. puberulum) and demonstrate the opportunities for restoring functional pathways using the genetic resources of wild species, resulting in production of healthier foods.  相似文献   
87.
This study reports on the destructuration of Wheat straw and Spruce wood cell walls after maceration in potassium carbonate or sodium hydroxide at pH = 10 in the presence of copper acetate. The alkaline treatments had a predominant impact on the wheat straw cell wall components over copper acetate. Either K-carbonate or Na-hydroxide extracted from wheat straw a particular lignin fraction rich in condensed C-C linkages, leading to the unmasking of new ether-linked sub-structures in the cell wall. This unmasking was increased in the presence of copper salt but only in the nonextracted Wheat straw sample incubated in carbonate and not in the corresponding extractive-free sample. This difference was related to the leaching of compounds from the nonextracted cell wall, which could sustain oxidative activity of copper by hindering its precipitation into inactive hydroxide and/or carbonate species. In Spruce wood samples, copper salt was the principal factor impacting on the lignin structure over alkali alone. Its effect was, however, only detected at the level of C-C linked dimers. These results confirmed that unmasking of lignin sub-structures also occurred in Spruce wood, but probably through mechanisms different from that evidenced in Wheat straw.  相似文献   
88.
The accumulation and speciation of Zn, Cu, Fe and Pb in the sediments of an artificial surface-flow wetland used to treat domestic wastewater near Christchurch, New Zealand, were examined. Water metal concentrations and total suspended solid (TSS) content were determined at the inflow and outflow both in winter and summer, and metal concentrations were analysed in shoots and roots of selected plants. Water and sediment data suggest that the wetland is acting as a sink for Zn, Cu and Pb, while Fe uptake is minimal and the wetland appears to be releasing Fe in the winter. Metal concentrations in the most mobile fractions (exchangeable and bound to carbonates) are negligible. Cu is mostly associated with the organic/sulphide phase, whereas Zn and Pb show a strong affinity for hydroxides and organics/sulphides. A large fraction of all metals is also present in the residual phase, and is therefore unlikely to be released into the overlying water. Metal concentrations are below the low trigger values of the ANZECC sediment quality guidelines, except for Pb near the inflow. However, results of sequential extraction suggest that a significant proportion of Pb occurs in the detrital phase and is therefore not bio-available. Metal concentrations were higher in the roots than in the shoots of both Juncus sp. and Lythrum hyssopifolia. This study shows that, although metal concentrations are low, TSS and metals, except Fe, are effectively removed by the wetland system. The low removal rate of Fe is possibly due to the young age of the wetland and low loading rates.  相似文献   
89.
Four trichothecene-producing strains of Fusarium graminearum were grown on three maize grain fractions, whole grain, degermed grain, and the germ, to determine the effect of natural substrates on mycotoxin production. Monitoring the ergosterol content after 25 days of incubation indicated that fungal growth on all grain fractions was comparable. Trichothecene (TCT) production was highest on degermed grain, less on whole grain, and very low or nondetectable on the germ; similar results were found with four different strains. It was concluded that inhibitor(s) of TCT biosynthesis were present in maize germ. The presence of phenolic compounds was investigated in the different fractions. The hydroxamate 4-acetylbenzoxazolin-2-one (4-ABOA), a known inhibitor of mycotoxin production, was found in the degermed and whole grain fractions but not in the germ. Therefore, the TCT inhibition observed on the maize germ fraction used in our study is clearly not linked to 4-ABOA. Other soluble phenolic compounds were found at a much higher concentration in the germ than in the two other fractions. The inhibition property of the soluble ester-bound extracts was tested in liquid culture. A possible role for these compounds is discussed.  相似文献   
90.
The occurrence of quartz particles having a platy morphology found in the white layers within the fossil forest deposits of Axel Heiberg Island, was further confirmed by optical and electron microscopic examinations. The platy quartz crystals were concentrated in the finer fractions. The δ 18O values of fractions enriched in platy quartz crystals ranged from +13.7 to +16.6 %0. These values were higher than those obtained for coarser fractions. The platy morphology and crystallographic character of the quartz particles concentrated in the finer size fractions are uncharacteristic both of aerosol derived material and of secondary silica. These findings and information available for the paleoenvironment of the study area favor a biogenic origin for the platy quartz. The oxygen-isotope data are consistent with this hypothesis.  相似文献   
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