Development and application of an indirect ELISA test for the detection of antibodies to Mycoplasma crocodyli infection in crocodiles (Crocodylus niloticus)     

Dawo F  Mohan K 《Veterinary microbiology》2007,119(2-4):283-289

Non-availability of a standardized rapid serodiagnostic test for quick and accurate diagnosis of Mycoplasma crocodyli (M. crocodyli) infection in crocodiles was the underlining reason for conducting the present study. An indirect enzyme-linked immunosorbent assay (iELISA) for the detection of antibodies (Ab) to M. crocodyli infection in crocodile sera was developed using sonicated antigen (Ag) and anti-crocodile conjugate. The iELISA test was optimised with different reagents and at different steps. A cut-off value of percent positive greater than or equal to 53.47% resulted in an estimated sensitivity and specificity of 85.67 and 100%, respectively. The developed iELISA could be used for detection of Abs to M. crocodyli infection in crocodiles and may enable to understand the transmission of the disease.  相似文献   

Therapeutic potential of Pirfenidone for treating equine corneal scarring          下载免费PDF全文

Michael K. Fink  Elizabeth A. Giuliano  Ashish Tandon  Rajiv R. Mohan 《Veterinary ophthalmology》2015,18(3):242-250

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Irrigation crop coefficients for lowland rice   总被引：1，自引：0，他引：1  

S. Mohan  N. Arumugam 《Irrigation and Drainage Systems》1994,8(3):159-176

Meteorological and lysimetric data for a period of nine years were used to develop crop coefficients for rice grown under lowland conditions in a sub-humid tropical climate in India. The estimated crop coefficients were found to be higher than those values recommended by FAO. A crop coefficient model with basal coefficient, moisture availability coefficient and surface wetness coefficient terms has been proposed and found suitable. On most counts, the moisture availability coefficient was found to be near unity and the wetness coefficient was found to be significant. The basal crop coefficients for lowland rice have also been presented for practical use with the proposed models.  相似文献   

Design and management nomograph for furrow irrigation     

D. Zerihun  J. Mohan Reddy  J. Feyen  G. Breinburg 《Irrigation and Drainage Systems》1993,7(1):29-41

There exist capabilities for analyzing the behavior of surface flow and the ultimate distribution of infiltrated water in furrow irrigation. The corresponding synthesis, i.e., the selection of appropriate combinations of inflow rates, cutoff times and length of furrow — design and management, currently not so well established, is treated herein. A design-management nomograph is proposed for free draining graded furrows. This is a plot of efficiency, time of cutoff and uniformity coefficient contours each given on a length-flow rate space adjacent to one another, for a furrow with given infiltration characteristics, flow geometry, slope, roughness and required depth of application. The nomograph can be used to determine the combinations of length, time of cutoff and flow rate that would yield in optimum combination of efficiency and uniformity.  相似文献   

乳极性脂质组成及其功能性研究进展     

李墨翰  宋婉莹  张秀敏  陈佳丽  热罕古丽·阿卜杜拉  刘爱成  于海坤  张娟  曹雪妍  岳喜庆 《乳业科学与技术》2022,45(2):24-29

乳中含有2％～5％的脂质,乳中的脂质由乳腺上皮细胞分泌并以乳脂肪球的形式分散在乳中.大部分乳脂(约98％)以甘油酯的形式存在于乳脂肪球内部,其余(约2％)包括甘油磷脂、鞘脂、糖脂等在内的少部分极性脂质则主要分布在乳脂肪球膜表面.乳极性脂质虽然在乳脂中占比较少,但却对哺乳动物的生长发育有着不可或缺的作用.本文首先介绍乳中...  相似文献   

HPTLC analysis of guggulsterone isomers in Commiphora wightii (Arn.) Bhandari: an endangered oleo-gum resin species heading towards extinction     

Alpana Kulhari  Arun Sheorayan  Navneet Saxena  Chander Mohan  Manisha Mangal  Ashok Chaudhury  Ashok K. Dhawan  Rajwant K. Kalia 《Genetic Resources and Crop Evolution》2013,60(3):1173-1180

The oleo-gum resin of Commiphora wightii (Arn.) Bhandari, a pharmacologically important balsamiferous woody shrub, has been used in treating various ailments and disorders since ancient times (2000 B.C.) due to the presence of steroidal compound guggulsterone. Two bioactive isomers of guggulsterone, E and Z, are responsible for lipid- and cholesterol-lowering and anti-cancerous activities. Further, guggul has been approved as food supplement by US-FDA as well as Council of Europe. Indiscriminate harvest of C. wightii from wild with negligible conservation efforts has lead to its inclusion in IUCN assemblage of endangered plant species. For identification of high guggulsterone yielding ecotypes of C. wightii, using high-performance thin-layer chromatographic (HPTLC) analysis, stem samples were collected from 50 plants from eleven locations in arid tracts of Haryana, Gujarat and Rajasthan. Dried, powdered material was subjected to extraction with petroleum ether using soxhlet apparatus. Samples were spotted on precoated activated silica plates (60F-254) and were developed using toluene–acetone (9:1 v/v) as mobile phase. The analysis was carried out in the absorbance mode at 250 nm using HPTLC scanner. The regression analysis data for the calibration plots for E and Z guggulsterone showed good linear relationship with R² = 1 and 0.9897, respectively. Highest concentration of guggulsterone E (284 μg/g dry wt) was found in the accession collected from Palana, Bikaner whereas highest guggulsterone Z concentration (89.5 μg/g dry wt) was found in the accession collected from CAZRI, Jodhpur.  相似文献   

Molecular mapping of QTLs for resistance to Fusarium wilt (race 1) and Ascochyta blight in chickpea (Cicer arietinum L.)     

Murali Mohan Sabbavarapu  Mamta Sharma  Siva Kumar Chamarthi  Nayakoti Swapna  Abhishek Rathore  Mahendar Thudi  Pooran Mal Gaur  Suresh Pande  Sarvjeet Singh  Livinder Kaur  Rajeev Kumar Varshney 《Euphytica》2013,193(1):121-133

Fusarium wilt (FW) and Ascochyta blight (AB) are two important diseases of chickpea which cause 100 % yield losses under favorable conditions. With an objective to validate and/or to identify novel quantitative trait loci (QTLs) for resistance to race 1 of FW caused by Fusarium oxysporum f. sp. ciceris and AB caused by Ascochyta rabiei in chickpea, two new mapping populations (F_2:3) namely ‘C 214’ (FW susceptible) × ‘WR 315’ (FW resistant) and ‘C 214’ (AB susceptible) × ‘ILC 3279’ (AB resistant) were developed. After screening 371 SSR markers on parental lines and genotyping the mapping populations with polymorphic markers, two new genetic maps comprising 57 (C 214 × WR 315) and 58 (C 214 × ILC 3279) loci were developed. Analysis of genotyping data together with phenotyping data collected on mapping population for resistance to FW in field conditions identified two novel QTLs which explained 10.4–18.8 % of phenotypic variation. Similarly, analysis of phenotyping data for resistance to seedling resistance and adult plant resistance for AB under controlled and field conditions together with genotyping data identified a total of 6 QTLs explaining up to 31.9 % of phenotypic variation. One major QTL, explaining 31.9 % phenotypic variation for AB resistance was identified in both field and controlled conditions and was also reported from different resistant lines in many earlier studies. This major QTL for AB resistance and two novel QTLs identified for FW resistance are the most promising QTLs for molecular breeding separately or pyramiding for resistance to FW and AB for chickpea improvement.  相似文献   

冷凝法油气回收流程模拟中的相平衡计算     

郭兰兰  孔祥强  王默晗 《油气储运》2013,32(1)

在冷凝法油气回收过程中,油气组分和含量不断变化,热物性如焓、熵、粘度、比热容等也相应发生改变.为了正确计算动态参数,给出了油气的相平衡计算模型.该模型由物料平衡条件方程组、热力学平衡条件方程组和状态方程结构体系3部分构成,并通过VC++语言编程求解.利用实际气体PR状态方程计算某油气成分在三级冷凝温度4℃、-50℃、110℃下的相平衡特性,与Aspen软件计算结果相比误差不大,可以作为后续流程模拟计算的基础.  相似文献   

Development and validation of a sensitive enzyme immunoassay (EIA) for blood plasma cortisol in female cattle,buffaloes, and goats     

R. Yadav  K. Mohan  V. Kumar  M. Sarkar  K. Nitu  H.H.D. Meyer  B.S. Prakash 《Domestic animal endocrinology》2013

A highly sensitive enzyme immunoassay (EIA) that used the second antibody coating technique and the cortisol-horseradish peroxidase conjugate as a label for determination of free and total cortisol in blood plasma of dairy animals (cows, buffaloes, and goats) was developed. For biological validation of the EIA, blood samples were collected from the animals at 48 and 24 h before and 0, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, and 132 h after dexamethasone administration. The EIA was performed directly with 20 μL of fresh plasma (for free cortisol) and also with 20 μL of heat-treated plasma (for total cortisol) after 1:5 dilutions with PBS. Cortisol standards ranging from 0.39 to 200 pg/well/20 μL were used, and the sensitivity of the EIA procedure was found to be 0.39 pg/well/20 μL, which corresponded to 0.02 ng/mL. In comparison with RIA the EIA was at least 4 times more sensitive and required 5 times less cortisol antiserum. In female cattle, buffaloes, and goats, the total, free, and bound plasma cortisol before dexamethasone administration was significantly (P < 0.05) higher than the total, free, and bound cortisol after dexamethasone administration. It can be concluded from these studies that the direct, sensitive EIA validated for estimating the free and total cortisol concentrations was sufficiently reliable and quick for studying the dynamics of cortisol distribution in blood plasma of dairy animals.  相似文献   

FISH CONSUMPTION AND FOOD SECURITY: A DISAGGREGATED ANALYSIS BY TYPES OF FISH AND CLASSES OF CONSUMERS IN SELECTED ASIAN COUNTRIES     

Madan Mohan Dey  Mohammed A. Rab  Ferdinand J. Paraguas  Somying Piumsombun  Ramachandra Bhatta  Md Ferdous Alam 《Aquaculture Economics & Management (Blackwell Science)》2013,17(1-2):89-111

ABSTRACT

This paper discusses fish consumption and preference patterns for fish species by income groups, and by urban/rural divide in Bangladesh, China, India, Indonesia, the Philippines, Thailand, and Vietnam. The analysis is based on primary data collected by the WorldFish Center and its partner institutes by means of a survey of 5,931 households in the selected countries. The FAO database and other published materials were also used to analyze trends in fish consumption. Freshwater fish species constitute a major share in total per capita fish consumption in most of these countries. Pelagic and demersal marine fish are the main contributor to per capita total fish consumption in the countries with longer coastal boundaries (such as Indonesia, the Philippines and Thailand), and in the coastal regions within each country. Results suggest that fish contributes between 15% and 53% of the total animal protein intake in these countries. Fish consumption varies widely with economic position of the households, in terms of both per capita consumption and type of fish species. Per capita fish consumption increases with increase in income. The share of fish protein in total animal protein expenditure is higher for lower income groups, demonstrating their dependence on fish as a source of animal protein. Poor people consume mostly low-price fish and rich people spend a significant portion of their fish budget on expensive fish. Per capita fish consumption is substantially higher in rural areas than in urban areas.  相似文献