茯苓液体培养条件研究及其营养浓缩液的相关检测   总被引：7，自引：0，他引：7  

王谦  闫蕾蕾  王超  冀宏  丁万杰  刘传斌  邢志华  李宝佳 《食用菌学报》2003,10(2):17-20

研究表明，液体培养条件下，茯苓经0～48h的适应期，48～144h的增殖期后进入稳定期，发酵液pH骤降。经检测，发酵液中含有包括十六烷酸和十八烷酸在内的有机酸；发酵罐培养120h，生物量≥30g/l00mL(鲜重)。茯苓营养浓缩液多糖含量≥5．0mg/mL，氨基酸含量≥480mg/100mL。动物实验证明，茯苓营养浓缩液具有显著的抗疲劳和提高机体免疫力作用，其重金属和微生物指标均符合国家标准。  相似文献   

糖类寄生螨快速分离检验方法   总被引：1，自引：0，他引：1  

黄世水  梁凤娇 《植物检疫》2003,17(1):15-16

本文对糖类寄生螨的快速分离检验方法进行了改进。将糖类溶解后，经过500目/英寸标准检验筛进行过筛分离。此方法对各种可溶性糖类中的寄生螨类能取得100％的快速分离检验结果。  相似文献   

"双链型"草-鹅-稻生态农业模式高产高效配套技术   总被引：11，自引：0，他引：11  

张家宏  王守红  白和盛  毕建花  张永新  唐宝国  孔佩  黄彩斌 《草业科学》2003,20(6):27-29

提出了“双链型”单-鹅-稻生态农业模式，总结了稻田套种牧草的方法和田间管理技术，探讨了种草与养鹅在时间上的相互衔接，以提高牧草的产量和利用率，进行了稻田套种牧草养鹅与种植小麦的比较效益分析。该配套技术的实施，为当前开展农业结构调整开辟了一条新路。  相似文献   

溴隐停对惠州麻肉种鸡产蛋性能的影响     

黄炎坤  程璞  王娟娟  司玉亭 《勤云标准版测试》2006,(12)

研究目的在于探讨多巴胺受体激动剂(溴隐停)对地方良种肉鸡繁殖性能的影响。方法从628只28周龄健康的“惠州麻”黄羽肉种鸡群中随机选取1组鸡笼内的40鸡作为实验组,其余的作为对照组。实验组鸡群喂服溴隐停,每只鸡每天1.25mg,连用4d,停用4d后再用4d,对照组不使用。结果在4周的实验期内实验组和对照组的产蛋率分别为83.86%和69.96%;破蛋率分别为1.28%和1.69%,畸形蛋率分别为4.33%和1.19%;实验组平均每只鸡的产蛋总数比对照组多3.89个、合格种蛋数多3.26个;药物处理对平均蛋重无显著影响;使用溴隐停能够提高鸡血液中的雌激素水平。结论溴隐停能够通过抑制地方良种肉鸡体内促乳素的合成与分泌,减少抱窝现象的发生,提高其繁殖性能。  相似文献   

中蜂品系间杂种优势利用研究初报     

张学锋  陈华生等 《中国蜂业》2001,52(4):7-9

经过冬蜜花期对以湖北鄂西中蜂为母本、广东粤东地中蜂为父本的杂交一代与本地中蜂进行比较，结果表明：杂交一代的繁殖力和产蜜量分别比本地中蜂高4．4％和11％。夏季乌桕花期对回交代进行对比结果表明：其繁殖力和产蜜量分别比本地中蜂高6％和21％。  相似文献   

加快淮北地区杂交粳稻发展的几个问题   总被引：2，自引：0，他引：2  

刘超 《杂交水稻》1995,(1):2-4

回顾了淮北地区十多年来杂交粳稻示范推广的发展过程，分析了影响杂交粳稻发展的主要原因，并论述了加快该地区杂交粳稻发展的几个主要问题。  相似文献   

2型猪链球菌的血清学鉴定   总被引：41，自引：0，他引：41  

黄毓茂  黄引贤 《中国兽医学报》1995,15(1):63-65

本试验首次证实了２型猪链球菌在我国的存在，采用玻片凝集、琼脂扩散及毛细管沉淀等３项试验，对１９９０年以来广东某猪场断奶仔猪暴发流行的败血症、脑膜炎及关节炎病猪中分离的１５株链球菌进行了血清学鉴定。结果表明：１５株链球菌均为２型猪链球菌；用高压法提取抗原进行沉淀反应，其结果比酸热法提取抗原具有简便、敏感、特异性强的特点。  相似文献   

“火罐”柿减压处理、低乙烯MA贮藏技术研究   总被引：5，自引：0，他引：5  

黄森  张继澍 《西北农业学报》1996,5(1):82-84

利用减压贮藏的原理,提出了减压处理低乙烯ＭＡ贮藏技术,并利用该技术对陕西优良柿子品种－－火罐柿进行了室温条件下的保鲜贮藏研究。结果表明,减压处理低乙烯ＭＡ贮藏技术甬地抑制火罐柿硬度的下降,延长果实贮藏期。保硬期为４３ｄ,比对照长２４ｄ;贮藏期达９７ｄ,比对照长６７ｄ。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

Ovipositor length of threeApocrypta species: Effect on oviposition behavior and correlation with syconial thickness     

Wen-Quan Zhen  Da-Wei Huang  Jin-Hua Xiao  Da-Rong Yang  Chao-Dong Zhu  Hui Xiao 《Phytoparasitica》2005,33(2):113-120

We investigated oviposition behavior in three non-pollinating fig wasps: the sympatric speciesApocrypta bakeri Joseph onFicus hispida L.,A. westwoodi Grandi onF. racemosa L., andApocrypta sp. onF. semicordata Buch.-Ham. The oviposition behavior differs significantly between one pair of species (A. bakeri andA. westwoodi) and the other species (Apocrypta sp. onF. semicordata).A. bakeri andA. westwoodi were similar in the following aspects: the posture of the abdomen and the action of the hind legs before penetration, and the bending ovipositor sheath during penetration. In contrast, the oviposition behavior ofApocrypta sp. is quite different. This difference can be explained by the significant correlation between ovipositor length and syconial thickness.Apocrypta sp. has a shorter ovipositor than the two other species, which correlates with the thinner syconial wall of its host figF. semicordata. It is deduced that the ovipositor length adapts to the syconial thickness and induces the oviposition behavior in the different species to diverge. For all threeApocrypta species, the midleg length and hindleg length are significantly correlated with their ovipositor lengths. This may be explained as due to the fact that body movement adjusting the hindlegs and midlegs up and down, or forward and backward, is also influenced by the ovipositor length.  相似文献