Genomewide association for a dominant pigmentation gene in sheep     

J.W. Kijas  M. Serrano  R. McCulloch  Y. Li  J. Salces Ortiz  J.H. Calvo  M.D. Pérez‐Guzmán  The International Sheep Genomics Consortium 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2013,130(6):468-475

Most published genomewide association studies (GWAS) in sheep have investigated recessively inherited monogenic traits. The objective here was to assess the feasibility of performing GWAS for a dominant trait for which the genetic basis was already known. A total of 42 Manchega and Rasa Aragonesa sheep that segregate solid black or white coat pigmentation were genotyped using the SNP50 BeadChip. Previous analysis in Manchegas demonstrated a complete association between the pigmentation trait and alleles of the MC1R gene, setting an a priori expectation for GWAS. Multiple methods were used to identify and quantify the strength of population substructure between black and white animals, before allelic association testing was performed for 49 034 SNPs. Following correction for substructure, GWAS identified the most strongly associated SNP (s26449) was also the closest to the MC1R gene. The finding was strongly supported by the permutation tree‐based random forest (RF) analysis. Importantly, GWAS identified unlinked SNP with only slightly lower p‐values than for s26449. Random forest analysis indicated these were false positives, suggesting interpretation based on both approaches was beneficial. The results indicate that a combined analytical approach can be successful in studies where a modest number of animals are available and substantial population stratification exists.  相似文献   

Treatment of cervical oesophageal rupture in horses     

C. M. Whitfield‐Cargile  P. C. Rakestraw  J. Hardy 《Equine Veterinary Education》2013,25(9):456-460

Oesophageal rupture in horses has only been previously described in detail in isolated case reports. The objectives of this study were to describe the clinical findings, specific treatment and outcome of oesophageal rupture in horses. Medical records of horses diagnosed with oesophageal rupture between 1994–2008 were reviewed. Clinical findings, treatment and outcome were recorded. Seven horses with cervical oesophageal perforations were included in the study. Two horses were subjected to euthanasia without treatment and 5 were treated surgically. Treatment involved a fasciotomy of the cervical musculature and oesophageal tube placement. Three of 5 horses survived long‐term (>one year). Our study showed that surgical treatment of cervical oesophageal rupture involving fasciotomy and oesophagostomy tube placement can be successful with 3/5 of treated horses surviving more than one year.  相似文献   

Management of cases suffering from atypical myopathy: Interpretations of descriptive,epidemiological and pathophysiological findings. Part 1: First aid,cardiovascular, nutritional and digestive care     

G. van Galen  D.‐M. Votion 《Equine Veterinary Education》2013,25(5):264-270

Atypical myopathy is highly fatal, but about a quarter of affected horses survive. This highlights the need for provision of supportive treatment for these cases. This review is a practical guideline for equine practitioners and includes suggestions for close monitoring of involved organ systems and discusses options of supportive treatment based on current knowledge of the condition.  相似文献   

The use of nuclear imaging for a mixed C cell microfollicular carcinoma of the thyroid gland in a mature horse     

M. N. Saulez  A. Viljoen  U. Kafka  L. Rubio‐Martinez  E. van Wilpe  J. Steyl 《Equine Veterinary Education》2013,25(3):118-125

A 15‐year‐old Boerperd stallion presented for thyroid enlargement associated with inappetance. Ultrasonographic examination of the thyroid gland revealed an enlarged left lobe, which, on cytological examination, contained numerous anaplastic cells. A mixed C cell microfollicular thyroid carcinoma was diagnosed following histopathological examination of the excisional biopsy specimen. In order to determine metastasis, pulmonary radiographs revealed a poorly‐marginated soft tissue opacity caudo‐dorsal to the cardiac silhouette. Thyroid and pulmonary scintigraphy was performed comparing ^99mTc‐sestamibi with ^99mTc‐pertechnetate and no metastasis was detectable. Following hemithyroidectomy, the stallion made a full recovery without the need for neoadjuvant chemotherapy. Six months later, repeated thyroid and pulmonary scintigraphy using ^99mTc‐sestamibi was normal.  相似文献   

A blinded,randomized, placebo‐controlled trial of the efficacy and safety of the Janus kinase inhibitor oclacitinib (Apoquel®) in client‐owned dogs with atopic dermatitis     

Sallie B. Cosgrove  Jody A. Wren  Dawn M. Cleaver  Kelly F. Walsh  Stacey I. Follis  Vickie I. King  Jezaniah‐Kira S. Tena  Michael R. Stegemann 《Veterinary dermatology》2013,24(6):587-e142

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Virus Detection in Questing Ticks is not a Sensitive Indicator for Risk Assessment of Tick‐Borne Encephalitis in Humans     

P. Stefanoff  M. Pfeffer  W. Hellenbrand  J. Rogalska  F. Rühe  A. Makówka  J. Michalik  B. Wodecka  A. Rymaszewska  D. Kiewra  A. Baumann‐Popczyk  G. Dobler 《Zoonoses and public health》2013,60(3):215-226

Tick‐borne encephalitis virus (TBEV) is the most important tick‐transmitted arbovirus causing human disease in Europe, but information on its endemic occurrence varies between countries because of differences in surveillance systems. Objective data are necessary to ascertain the disease risk for vaccination recommendations and other public health interventions. In two independent, separately planned projects, we used real‐time RT‐PCR to detect TBE virus in questing ticks. In Poland, 32 sampling sites were selected in 10 administrative districts located in regions where sporadic TBE cases were reported. In Germany, 18 sampling sites were selected in two districts located in a region with high TBE incidence. Altogether, >16 000 ticks were tested by real‐time RT‐PCR, with no sample testing positive for TBEV. A systematic search for published studies on TBEV prevalence in ticks in Poland and Germany also suggested that testing large numbers of collected ticks could not consistently assure virus detection in known endemic foci. Although assignment of results to administrative regions is essential for TBE risk mapping, this was possible in only 10 (investigating 22 417 ticks) of 15 published studies (>50 000 ticks) identified. We conclude that the collection and screening of ticks by real‐time RT‐PCR cannot be recommended for assessment of human TBE risk. Alternative methods of environmental TBEV monitoring should be considered, such as serological monitoring of rodents or other wildlife.  相似文献   

Distribution and Genotypic Characterization of Campylobacter jejuni Isolated from Poultry in Split and Dalmatia County,Croatia     

A. Kovačić  I. Listeš  C. Vučica  L. Kozačinski  I. Tripković  K. Šiško‐Kraljević 《Zoonoses and public health》2013,60(4):269-276

Consumption of poultry contaminated with Campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. Therefore, the aim of our study was to investigate the prevalence and genotype diversity of Campylobacter jejuni in poultry meat intended for consumption in Split and Dalmatia County, which is the second biggest County in Croatia. Furthermore, we also wanted to discover possibly stable clones of C. jejuni appearing in different samples and periods of time, which would indicate their ability to persist in or adapt to poultry. In the period from March 2008 until June 2010, 834 samples of poultry from various sources were examined using a surface swab technique. Isolation of C. jejuni was performed by Preston broth and Karmali agar. Identification of the isolates was carried out using biochemical tests. C. jejuni was found in 84 of 574 chicken samples (14.6%) and in nine of 260 samples of turkey (3.5%). Pulse‐field gel electrophoresis (PFGE) was used to analyse 61 obtained isolates using SmaI and KpnI. Of 22 different macrorestriction profiles (MRP) that were found, five were detected in poultry from both different locations and periods of time. Samples from 11 locations were found to be contaminated with more than two different genotypes of C. jejuni. Interestingly, the same MRP were found both in poultry declared to be of domestic origin and in the poultry imported from abroad. The prevalence of C. jejuni in poultry samples was in accordance with previously reported results. Genotypic analysis indicated that the population of C. jejuni in Split and Dalmatia County was diverse and that multiple strains of C. jejuni could be found in the same poultry samples. Furthermore, the same genotypes were identified from the samples obtained from different locations and periods of time, which could support the theory of a global existence of certain MRP that are able to persist in or adapt to poultry.  相似文献   

Investigations of the Distribution and Persistence of Salmonella and Ciprofloxacin‐Resistant Escherichia coli in Turkey Hatcheries in the UK     

D. Mueller‐Doblies  C. Clouting  R. H. Davies 《Zoonoses and public health》2013,60(4):296-303

This study aimed at gaining information on the presence of Salmonella in UK turkey hatcheries and possible epidemiological links between breeding farms, hatcheries and finishing farms. The presence of ciprofloxacin‐resistant E. coli in hatchery samples, as well as in faecal samples from farms, and trends in occurrence of resistance were also investigated. Over a 2 year‐period, four British turkey hatcheries were visited and intensively sampled for the presence of Salmonella and ciprofloxacin‐resistant E. coli. In two hatcheries, a link could be demonstrated between the presence of certain Salmonella serovars in the hatcheries and on breeding and finishing farms. Within the hatcheries, serovars linked to breeding farms were found more frequently in the poult processing and dispatch areas, whereas serovars identified as ‘resident hatchery contaminants’ were predominantly found inside the hatcher cabinets. Ciprofloxacin‐resistant isolates of S. Senftenberg were identified in one hatchery, which coincided with enrofloxacin treatment of some of the breeding flocks. Ciprofloxacin‐resistant E. coli was found in two hatcheries, and the majority of these isolates showed multidrug resistance.  相似文献   

Multilocus Sequence Types of Environmental Campylobacter jejuni Isolates and their Similarities to those of Human,Poultry and Bovine C. jejuni Isolates     

C. P. A. de Haan  K. Lampén  J. Corander  M.‐L. Hänninen 《Zoonoses and public health》2013,60(2):125-133

In this study, we investigated the multilocus sequence type (MLST) diversity and population genetics of Campylobacter jejuni isolates collected from the natural waters (n = 57), wild birds (n = 37) and zoo animals (n = 19) in southern Finland, the Helsinki area and the Helsinki Zoo, respectively. On average, we found C. jejuni in 20%, 10.4% or 11.5% of the samples collected from natural waters, wild birds and zoo animals, respectively. High ST diversity was detected in all three sources and 41.2% of the STs were novel, but the multi‐host adapted ST‐45 was the most common ST detected. The MLST data, supplemented with C. jejuni isolates from domestically acquired human infections (n = 454), poultry (n = 208) and bovines (n = 120), were utilized in a population structure study. The results indicate four groups of strains with varying ecological associations, demonstrating presence of genetically distinct lineages within each of the studied sources. We discovered that the greatest ST overlap occurs between human isolates and isolates from natural waters and poultry, which suggests that the latter two are the most important sources of C. jejuni among domestically acquired infections in Finland.  相似文献   

Two Cases of Cutaneous Diphtheria Associated with Occupational Pig Contact in Germany     

R. Konrad  T. Schmidt‐Wieland  S. Hörmansdorfer  M. Eddicks  A. Sing 《Zoonoses and public health》2013,60(8):539-542

In 2010, two independent cases of cutaneous diphtheria caused by toxigenic C. ulcerans were identified in Germany. Both patients had intense occupational contact with pigs. Diagnostic work‐up comprising biochemical differentiation, rpoB sequencing, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF) analysis, real‐time tox PCR and Elek test as well as public health measures including an intensified source tracing involving 83 asymptomatic pigs of an associated pig farm are presented.  相似文献