Variation in the host preferences and responses of <Emphasis Type="Italic">Ascia monuste orseis</Emphasis> Godart (Lepidoptera: Pieridae) to cultivars of collard greens <Emphasis Type="Italic">Brassica oleracea</Emphasis> (L.) var. <Emphasis Type="Italic">acephala</Emphasis>     

Eunice Cláudia Schlick-Souza  Edson Luiz Lopes Baldin  André Luiz Lourenção 《Journal of pest science》2011,84(4):429-436

This work evaluated the preference of Ascia monuste orseis among 29 cultivars of collard greens through free- and no-choice assays using female adults (attractiveness and oviposition) and third-instar larvae (feeding preference), in both laboratory and greenhouse conditions. In free-choice tests with female adults, the L, V, Y, H, A, and Z cultivars produced oviposition non-preference in butterflies. Cultivars R, P, C, and D produced feeding non-preference in third-instar larvae of A. monuste orseis in free- and no-choice tests. Our findings may be useful for genetic improvement focusing on the development of B. oleracea var. acephala cultivars resistant to A. monuste orseis.  相似文献   

Comparison of experimental methods for measuring infinite dilution volatilities of aroma compounds in water/ethanol mixtures     

Athès V  Peña y Lillo M  Bernard C  Pérez-Correa R  Souchon I 《Journal of agricultural and food chemistry》2004,52(7):2021-2027

Several experimental methodologies exist for measuring volatilities; however, results show great dispersion and sometimes lack of agreement between different methods. The aim of our study was to compare the performance of three static headspace methods (vapor phase calibration, VPC; phase ratio variation, PRV; and liquid calibration static headspace, LC-SH) for determining gas/liquid partition coefficients of two aroma compounds in hydroalcoholic multicomponent solutions at infinite dilution. Comparison with literature data based on static and dynamic methods showed that PRV is simpler than VPC and LC-SH and that VPC and PRV are more accurate than LC-SH, which presented a significant bias (50% lower values).  相似文献   

Regulation of lysine metabolism and endosperm protein synthesis by the opaque-5 and opaque-7 maize mutations   总被引：3，自引：0，他引：3  

Azevedo RA  Lea PJ  Damerval C  Landry J  Bellato CM  Meinhardt LW  Le Guilloux M  Delhaye S  Varisi VA  Gaziola SA  Gratão PL  Toro AA 《Journal of agricultural and food chemistry》2004,52(15):4865-4871

Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.  相似文献   

Presence of porcine parvovirus in sera from pigs is independent of antibody titers     

Streck AF  Gava D  Souza CK  Gonçalves KR  Bortolozzo FP  Wentz I  Canal CW 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(5-6):242-246

Porcine parvovirus (PPV) is a widespread DNA virus that causes reproductive failure in swine. The aim of the present study was to investigate the presence of PPV in sera of nursery piglets (healthy n = 191 and wasting n = 132) and regularly vaccinated sows (with different parity rank [PR] n = 129), collected from different herds. Altogether, 452 animals were sampled in 27 herds owned by five companies. All sera were analyzed for the presence of PPV DNA by nested-PCR. The samples from sows were in addition tested for the presence of antibodies by Hemagglutination Inhibition (HI). PPV DNA was detected in healthy piglets (15.7%), wasting piglets (18.2%) and sows (17.8%). 25 herds had at least one positive sample and four companies had positive animals. The serology revealed that 84.7% of the sows had detectable antibodies and the fourth PR sows had the highest mean PPV antibody titers. Thirteen sows (19.1%) were found to be positive for DNA detection in the presence of high levels of antibody titers (> 512). This finding indicates that PPV DNA can be detected in different swine production categories irrespective of antibody titers.  相似文献   

Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions     

Monte LG  Conceição FR  Coutinho ML  Seixas FK  da Silva EF  Vasconcellos FA  deCastro LA  Hartleben CP  Dellagostin OA  Aleixo JA 《Comparative immunology, microbiology and infectious diseases》2011,34(5):441-446

Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2–7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 10⁷ M⁻¹ to 4 × 10⁸ M⁻¹. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.  相似文献   

Mechanistic model to understand in vivo salt release and perception during the consumption of dairy gels     

de Loubens C  Saint-Eve A  Déléris I  Panouillé M  Doyennette M  Tréléa IC  Souchon I 《Journal of agricultural and food chemistry》2011,59(6):2534-2542

The objective of this study was to develop a model to simulate salt release during eating. Salt release kinetics during eating was measured for four model dairy products with different dynamic salty perceptions. A simple in vivo model of salt release was developed to differentiate between the contribution of the individual and of the product to salt release. The most difficult model parameter to determine or predict is the evolution of the contact area between the product and the saliva. Fitting the model to the experimental data showed that the subject's masticatory performance and fracture initiation energy of the product determined the contact area between the product and the saliva generated by mastication. Finally, the role of release dynamics on sensory time-intensity profiles is discussed.  相似文献   

Phenotypic and genetic characterization of Salmonella enterica subsp. enterica serovar Typhimurium isolated from pigs in Rio Grande do Sul, Brazil     

Bessa MC  Michael GB  Canu N  Canal CW  Cardoso M  Rabsch W  Rubino S 《Research in veterinary science》2007,83(3):302-310

This study aimed to investigate the relatedness of porcine Salmonella enterica subsp. enterica (S.) serovar Typhimurium strains isolated in Southern Brazil. Sixty-six isolates from pigs belonging to three commercial companies were submitted to phage typing, XbaI-macrorestriction (PFGE), IS200 hybridization, rep-PCR, antimicrobial susceptibility testing, and PCR assay targeting the spvR region. All strains presented a unique rep-PCR pattern and 63 strains had a common IS200 profile. One pulse-type (XA) was the most prevalent (39/66 strains) and included strains of phage types DT177, DT192, DT194 and RDNC. The spvR region was detected in three strains, which harboured plasmids of 90 kb. High rates of tetracycline, sulfonamide and streptomycin resistance were found. Isolates from farms located in different geographic regions but associated to the same commercial companies clustered together and presented a common resistance profile. Results suggested that clonal groups of S. Typhimurium are present in pig commercial companies in Southern Brazil.  相似文献   

Intra-specific variability of virulence in Leishmania infantum zymodeme MON-1 strains     

Baptista-Fernandes T  Marques C  Roos Rodrigues O  Santos-Gomes GM 《Comparative immunology, microbiology and infectious diseases》2007,30(1):41-53

This study aims to characterize the intra-specific variability of virulence in Leishmania infantum zymodeme MON-1 strains isolated from dogs and immunocompetent and immunosuppressed patients through the evaluation of growth pattern, infective ability and immunopathogenicity. Two of the strains, classified as the most virulent, presented higher levels of macrophage infection, increased promastigote replication in culture medium and as well as amastigote multiplication within macrophages. These strains caused the most pathogenic infection inducing splenomegalia and maximum parasite loads in spleen and liver of BALB/c mice. The other strains exhibited either low virulence, with reduced infective capability and low replication levels, or an intermediate virulent phenotype showing mixed features similar to low and high virulent phenotypes. A correlation between the infectivity, growth dynamics and pathogenicity of each strain and the humoral and cellular immune response was demonstrated. Strains with accentuated virulent phenotype induced higher levels of anti-Leishmania IgG1 antibodies and TGF-beta but reduced production of IFN-gamma. Virulence phenotype seems to be a characteristic of each strain regardless of the host (dog or human) from which it was firstly isolated.  相似文献   

Hypertrophic osteopathy associated with renal pelvis transitional cell carcinoma in a dog     

Grillo TP  Brandão CV  Mamprim MJ  de Jesus CM  Santos TC  Minto BW 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(7):745-747

A 6-year-old male, Belgian shepherd dog was presented with lethargy, oliguria, hematuria, and reluctance to move. The dog developed hypertrophic osteopathy secondary to renal pelvis transitional cell carcinoma. A nephrectomy was performed and after a year, the dog was completely asymptomatic, and no evidence of metastatic disease was present.  相似文献   

Genetic diversity and population structure in a regional collection of Kersting’s groundnut (Macrotyloma geocarpum (Harms) Maréchal & Baudet)     

Kafoutchoni  Konoutan M.  Agoyi  Eric E.  Agbahoungba  Symphorien  Assogbadjo  Achille E.  Agbangla  Clément 《Genetic Resources and Crop Evolution》2021,68(8):3285-3300

Genetic Resources and Crop Evolution - Kersting’s groundnut (Macrotyloma geocarpum (Harms) Maréchal &amp; Baudet) is an important source of protein and essential nutrients that...  相似文献