Development of microsatellite markers and evaluation of genetic diversity of the Amazonian ornamental fish Pterophyllum scalare     

Pandolfi  Victor César Freitas  Yamachita  Andrei Lincoln  de Souza  Felipe Pinheiro  de Godoy  Sara Mataroli  de Lima  Ed Christian Suzuki  Feliciano  Daniele Cassiano  de Pádua Pereira  Ulisses  Povh  Jayme Aparecido  Ayres  Denise Rocha  Bignardi  Annaiza Braga  Penafort  Jefferson Murici  de Fátima Ruas  Claudete  Lopera-Barrero  Nelson Mauricio 《Aquaculture International》2021,29(6):2435-2449

The objective of the present work was to develop species-specific microsatellite markers for P. scalare and to analyze the diversity and genetic structure of a wild population, from the Amazon River, and three commercial stocks (common, marble, and clown morphological variants), from farmers in Vieras-Minas Gerais. Through microsatellite-enriched genetic libraries, 11 microsatellite markers with adequate amplification patterns were characterized. Population genetic analysis identified eight polymorphic loci that generated 66 alleles ranging from two alleles (PSCA1B3) to nine (PSCA2H1). The polymorphic information content ranged from 0.031 to 0.827. High genetic differentiation was observed between the wild population and the stocks, and moderate differentiation between the three stocks. Deviation in the Hardy-Weinberg equilibrium was observed in one locus in the wild population, in five loci in the common morphological variant, in two in the marble, and in two in the clown morphological variant. Bayesian analysis of genetic structure revealed the existence of two clusters, one represented by the natural population and the other by the stocks. The developed microsatellite markers serve as a tool for the analysis of diversity and genetic structure and conservation studies of P. scalare.

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Canine cutaneous neosporosis in Brazil          下载免费PDF全文

Thais R. Mann  Gustavo C. Cadore  Giovana Camillo  Fernanda S. F. Vogel  Claudete Schmidt  Cinthia M. Andrade 《Veterinary dermatology》2016,27(3):195-197

The clinical signs of infection in dogs with Neospora caninum are usually associated with neurological disorders and are seen in young dogs. In this brief case report we observed multifocal ulcerative and exudative skin nodules on the neck and pelvic limbs of a 10‐year‐old cocker spaniel dog. Infection with N. caninum was diagnosed on the basis of cytology and examination of skin tissues by PCR. The dog initially responded to treatment with clindamycin and then relapsed; the dog died. Infection with N. caninum may have been due in part to immune suppression due to hyperadrenocorticism; which either allowed for the development of a primary infection or reactivation of a latent infection by N. caninum with the occurrence of skin lesions.  相似文献   

Analysis of multicriteria in the practice of environmental services in areas of watersheds     

Claudete Bezerra dos Santos Canada  Carlos Alberto Mariottoni 《Journal of Sustainable Forestry》2013,32(7):500-514

ABSTRACT

Watersheds have become strategically important for the survival of cities due to the accelerated increase in both the demand and the rapid deterioration of the water intended to supply the urban populations. The present article aims to present the applicability of the “multicriteria method” to help the Government of the State of Sao Paulo, Brazil organize payment for environmental services for watershed protection using the “Delphi method” as the principal methodology. This article consists of a review of the literature and a case study of the Mina d’Água program from the State Secretary of the Environment (within the Government of São Paulo) in partnership with other municipalities (city by river basin). The article also covers the advent of pertinent climate change laws. The positive results in the use of the Delphi method represent a fruitful tool to facilitate communication between groups of experts and the public, encouraging better judgment and a more consistent decision-making process.  相似文献   

Virulence characteristics of Escherichia coli isolates obtained from broiler breeders with salpingitis     

Monroy MA  Knöbl T  Bottino JA  Ferreira CS  Ferreira AJ 《Comparative immunology, microbiology and infectious diseases》2005,28(1):1-15

Thirty isolates of Escherichia coli from broiler breeders with salpingitis were studied. Using the slide agglutination test, the isolates were found to belong to serogroups O1, O2, O5, O36, O45, O53 and O78. Pathogenicity for day-old chicks was determined by air sac inoculation and isolates were categorized as having high, intermediate or low virulence. Growth on iron starvation medium was observed together with aerobactin production. Based on the results of in vitro adherence tests, attachment to oviduct epithelium from old birds was found to be superior to that observed using corresponding material from young birds. DNA hybridization testing for type 1, P, and S fimbriae revealed predominant expression of type 1, correlating with mannose-sensitive hemagglutination using guinea-pig erythrocytes. In this study, P and S fimbriae were not considered to be important adherence factors. Study findings would suggest that, as far as salpingitis is concerned, type 1 fimbriae can play an important role in E. coli infection in breeders. An interesting result to emerge from the study was the observation that E. coli isolates were completely resistant to serum from young breeders, whereas they were completely sensitive using serum from older breeders. Based on serogroups involved, pathogenicity for day-old chicks and virulence indicators, the salpingitis isolates were similar to those from cases of chronic respiratory disease.  相似文献   

Detection of enteric pathogens in Turkey flocks affected with severe enteritis,in Brazil     

Joelma Moura-Alvarez  Luis F. N. Nuñez  Claudete S. Astolfi-Ferreira  Terezinha Knöbl  Jorge L. Chacón  Andrea M. Moreno  Richard C. Jones  Antonio J. Piantino Ferreira 《Tropical animal health and production》2014,46(6):1051-1058

Twenty-two flocks of turkeys affected by enteric problems, with ages between 10 and 104 days and located in the Southern region of Brazil, were surveyed for turkey by PCR for turkey astrovirus type 2 (TAstV-2), turkey coronavirus (TCoV), hemorrhagic enteritis virus (HEV), rotavirus, reovirus, Salmonella spp., and Lawsonia intracellularis (Li) infections. Eleven profiles of pathogen combination were observed. The most frequently encountered pathogen combinations were TCoV-Li, followed by TCoV-TAstV-2-Li, TCoV-TastV-2. Only TCoV was detected as the sole pathogen in three flocks. Eight and 19 flocks of the 22 were positive for TAstV-2 and TCoV, respectively. Six were positive for Salmonella spp. and L. intracellularis was detected in 12 turkey flocks. Reovirus and HEV were not detected in this survey. These results throw new light on the multiple etiology of enteritis in turkeys. The implications of these findings and their correlation with the clinical signs are comprehensively discussed, illustrating the complexity of the enteric diseases.  相似文献