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61.
Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil 总被引:2,自引:0,他引:2
62.
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64.
Kleinschmidt S Meneses F Nolte I Hewicker-Trautwein M 《Research in veterinary science》2008,84(1):41-48
The distribution and numbers of leucocytes and mast cells (MC) in the canine gastrointestinal tract of three different age groups was investigated immunhistochemically. In all age groups, CD3+ T cells were more prominent in the villus region than in the crypt areas without differences between intestinal segments, whereas macrophages were more randomly distributed. Kresylecht-violet and tryptase-positive MC were prominent in pericrypt regions with statistic significances. Chymase-bearing mast cells, IgA-, IgG- and IgM-containing cells did not show significant differences in their distribution but, except for IgG-positive cells, subjective trends with increasing numbers towards the crypts exist. The reasons for the distribution of T cells, macrophages, immunoglobulin-containing cells and mast cells are not clear. Lamina propria CD3+ T cells and macrophages significantly decreased whilst a significant increase of IgA-containing plasma cells with increasing age was found. For mast cell subtypes, IgG- and IgM-containing cells no significant changes in numbers with increasing age exist. 相似文献
65.
Riondato F D'Angelo A Miniscalco B Bellino C Guglielmino R 《Veterinary journal (London, England : 1997)》2008,175(3):364-368
The effect of transportation on peripheral blood lymphocyte subsets in 24 calves was investigated by flow cytometry. Blood was collected before departure, on arrival, at 24h and 1 week after arrival. Highest leucocyte and neutrophil counts, associated with increased concentrations of cortisol and catecholamines, indicated that stress was maximal upon arrival. At this time, a decrease in the percentages of all T lymphocyte subsets was evident, while they did not decrease as absolute counts. The proportion of CD21(+) cells did not change, indicating that the relative reduction of T lymphocyte subsets was not related to an increase in B lymphocytes. These variations may be due to the increase of a natural killer (NK) cell subset. NK cell expansion, together with increasing lymphocyte count and increasing major histocompatibility complex class II expression, may indicate stress-induced stimulation of the immune system. 相似文献
66.
Bussadori R Bussadori C Millán L Costilla S Rodríguez-Altónaga JA Orden MA Gonzalo-Orden JM 《Veterinary journal (London, England : 1997)》2008,176(2):221-226
This article describes the treatment of single congenital portosystemic shunts (CPSs) (intrahepatic and extrahepatic) using an interventional radiology technique involving embolisation of anomalous vessels with percutaneous coils. Briefly, a multipurpose catheter was introduced into the caudal vena cava and then into the portosystemic shunt. An autoexpandable stent was placed in the caudal vena cava, next to the shunt, in order to avoid coil migrations, and a cobra-like vascular catheter was used to pass through the stent and to place the coils in the shunt. This technique was used for treatment of CPS in six dogs. The results indicate that percutaneous embolisation of a CPS using coils, a less invasive technique than the traditional surgical technique, may result in complete closure of the anomalous vessel without development of portal hypertension. 相似文献
67.
Context
Anthropogenic landscape simplification and natural habitat loss can negatively affect wild bees. Alternatively, anthropogenic land-use change may diversify landscapes, creating complementary habitats that maintain overall resource continuity and diversity.Objectives
We examined the effects of landscape composition, including land-cover diversity and percent semi-natural habitat, on wild bee abundance and species richness within apples, a pollinator-dependent crop. We also explored whether different habitats within diverse landscapes can provide complementary floral resources for bees across space and time.Methods
We sampled bees during apple bloom over 2 years within 35 orchards varying in surrounding landscape diversity and percent woodland (the dominant semi-natural habitat) at 1 km radii. To assess habitat complementarity in resource diversity and temporal continuity, we sampled flowers and bees within four unique habitats, including orchards, woodlands, semi-natural grasslands, and annual croplands, over three periods from April–June.Results
Surrounding landscape diversity positively affected both wild bee abundance and richness within orchards during bloom. Habitats in diverse landscapes had different flower communities with varying phenologies; flowers were most abundant within orchards and woodlands in mid-spring, but then declined over time, while flowers within grasslands marginally increased throughout spring. Furthermore, bee communities were significantly different between the closed-canopy habitats, orchards and woodlands, and the open habitats, grasslands and annual croplands.Conclusions
Our results suggest that diverse landscapes, such as ones with both open (grassland) and closed (woodland) semi-natural habitats, support spring wild bees by providing flowers throughout the entire foraging period and diverse niches to meet different species’ requirements.68.
Undesirable characteristic of rapeseed oil is a relatively high level of linolenic acid (18:3), which is easily oxidized leading
to rancidity and a shortened shelf life of the oil. Previous attempts to reduce linolenic acid levels in rapeseed oil through
breeding have been impaired by complex genetics and strong environmental sensitivity of this trait. Therefore, our objective
was to develop molecular markers for low linolenic acid that could facilitate the breeding of low linolenic rapeseed. Bulked
segregant analysis was employed to identify two RAPD markers associated with 18:3 in a doubled haploid population segregating
for linolenic and erucic acid levels. Based on analysis of individual DH lines, the markers RM350 and RM574, representing
two independent loci, accounted for a total of 39% of the genetic variability in this population. This marker RM350 alone
accounted for 25% genetic variation for this trait with no evidence of recombination. Significant interlocus interaction found
between the markers RM350 and RM574 suggested that epistasis was involved in the genetic control of 18:3 level in this population.
Another marker designated as RM322, which was independent of the other two, was found significantly associated with the erucic
acid level and oil content. RAPD markers identified in this study should be a useful tool for the early detection of low linolenic,
or low or high erucic acid genotypes in rapeseed breeding programs based on doubled haploids.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
69.
A route for the immobilization of 2,4‐dichlorophenoxyacetic acid (2,4‐D) on silica gel has been developed. In the first step the precursor was prepared by reacting the silylating agent 3‐(trimethoxysilyl)propylamine with silica gel. Nitrogen analysis of this anchored compound showed the presence of 1.16 mmol of amine groups per gram of support. The herbicide was covalently bonded to the amine groups previously anchored onto silica gel. Infrared, [13C] and [29Si] NMR spectra supported the reaction between the nitrogen of the amine group of the anchored silica with the carbon on the para‐position of the aromatic ring of 2,4‐D. The reaction yield, 90.3%, was confirmed through elemental analysis. © 2000 Society of Chemical Industry 相似文献
70.
C. lvarez‐Claudio P. García‐Rovs R. Ocharan J.A. Cabal F.J. Ocharan M.A. lvarez 《水产资源保护:海洋与淡水生态系统》2000,10(2):93-102
1. A population of the freshwater pearl mussel Margaritifera margaritifera L. was found in the River Narcea in the province of Asturias (north‐western Spain). Primary analysis of the population shows that it is well distributed along the river and is particularly abundant in shady river banks with a gravel substrate. 2. The length of living mussels ranges between a minimum of 22 mm and a maximum of 116 mm, with most in the size class between 65 mm and 70 mm. There is a good proportion of young mussels, although no fertile females were found in the course of the investigation. 3. Although there is no specific legislation in Spain for the protection of M. margaritifera, it has recently been proposed to include M. margaritifera in the Spanish List of Threatened Species as ‘vulnerable’. 4. Although the River Narcea seems to provide suitable conditions for the pearl mussel (water quality, available salmonid hosts, etc.), the main conservation measures must be directed at avoiding river fragmentation from dams, channelization and organic enrichment from individual farms or towns situated close to the river bank. Copyright © 2000 John Wiley & Sons, Ltd. 相似文献