A simplified PCR method for genotyping Mycoplasma mycoides subspecies mycoides small colony: the aetiologic agent of contagious bovine pleuropneumonia     

Churchward CP  Hlúšek M  Nicholas RA  Ayling RD  McAuliffe L 《Veterinary microbiology》2012,159(1-2):257-259

Mycoplasma mycoides subspecies mycoides small colony is the aetiological agent of contagious bovine pleuropneumonia, a cattle disease endemic to areas of sub-Saharan Africa. Twenty isolates from various geographical locations and the type strain were analysed by multi-locus sequence analysis (MLSA). The data generated was then used to develop three PCR primer sets to differentiate these isolates. The PCRs differentiated the isolates into four groups; the type strain (T); isolates of European origin (Eu); isolates from Tanzania (Af1) with a final group consisting of isolates from Namibia and Botswana (Af2). These PCRs offers a rapid and efficient post-identification typing method without the need to sequence and analyse multiple genes.  相似文献   

Diameter and death of whorl and interwhorl branches in Atlas cedar (<Emphasis Type="Italic">Cedrus atlantica</Emphasis> Manetti): a model accounting for acrotony     

François Courbet  Jean-Christophe Hervé  Etienne K. Klein  Francis Colin 《Annals of Forest Science》2012,69(2):125-138

• Introduction   

Branch size and branch status (dead or alive) are important characteristics closely related to tree growth and wood quality. The aim of this study was to design models for the diameter and status of branches in Atlas cedar (Cedrus atlantica Manetti).  相似文献   

Native plant enthusiasm reaches new heights: Perceptions,evidence, and the future of green roofs     

Colleen Butler  Erin Butler  Colin M. Orians 《Urban Forestry & Urban Greening》2012,11(1):1-10

The use of native plants on green roofs has attracted considerable attention in recent years. With this comes the implicit assumptions that native plants are better adapted, provide greater environmental benefit, and are more aesthetically pleasing than non-native plants. We examined papers published in scholarly journals and papers presented at the annual North American green roof conference to identify who is promoting the use of native plants on green roofs, their rationale for doing so, and the scientific evidence to support the assertion that natives are better adapted. Architects, landscape architects, and biologists were the most likely to promote native plants and engineers were the least likely. Many of the reasons for using native plants on green roofs originate from ground-level landscaping and have simply been transplanted to the roof, without regard for the fact that the rooftop is a fundamentally different environment than the ground. Nearly half of all pro-native papers used the term “native” without definition. This review highlights the need for greater rigor and transparency when promoting the use of native plants and further demonstrates how misconceptions can result in sub-optimal green roof design and performance.  相似文献   

Waxy durum acts as a unique form of crumb softener in bread     

Julie Lafaye  Heiterani LeeLaura Blake  Colin Jenner 《Journal of Cereal Science》2013

Flour milled from waxy durum grain was incorporated into bread formulations and its effects on crumb softness and loaf volume compared to those of added fats and emulsifiers. Waxy durum had a small effect on increasing loaf volume, and little of this effect could be explained by dough height or oven spring; effects of most other additives were closely related to dough height or oven spring. For all additives, the relationship between crumb softness and loaf volume fitted a power law relationship. Waxy durum and some of the additives had a greater softening effect than predicted from their effect on volume while others produced a much firmer crumb. With only one exception, when combined with other additives waxy durum reduced compression of the crumb more than predicted on the basis of the effects on loaf volume of the additives alone. In common with some of the added lipids, during compression analysis, crumb prepared with waxy durum flour had similar levels of structural damage as crumb baked from the standard formulation; other lipids and emulsifiers produced more fragile crumb. Waxy durum flour appears to have unique effects on crumb softness.  相似文献   

Detection of Streptococcus equi subspecies equi using a triplex qPCR assay     

Katy Webb  Colin Barker  Tihana Harrison  Zoe Heather  Karen F. Steward  Carl Robinson  J. Richard Newton  Andrew S. Waller 《Veterinary journal (London, England : 1997)》2013,195(3):300-304

Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.  相似文献   

Track Counts as a Means of Estimating Population Densities of Bushbuck     

P. B. Odendaal  M. J. Cameron  A. J. Priday 《Southern Forests》2013,75(1):65-68

Counts of bushbuck tracks were used to estimate their population densities. Good correlations were obtained between various indices of track frequency and estimated absolute population density. The technique is therefore recommended for assessing the absolute density of a population. High sampling costs, however, disqualify the technique for detecting small changes in density.  相似文献   

Diagnosis of brucellosis by agglutinins in milk     

H.S. Cameron D.V.M. Ph.D. 《New Zealand veterinary journal》2013,61(3):41-44

Extract

First let me express my appreciation of being the recipient of a Fulbright Fellowship for research in New Zealand and also for the privilege of discussing brucellosis before the New Zealand Veterinary Association.  相似文献   

Feature Review Series     

Dr Colin Mackintosh 《New Zealand veterinary journal》2013,61(6)

Extract

This issue of the New Zealand Veterinary Journal contains our second Feature Review Series, this time comprising six state-of- the-art reviews on the Health and Welfare of Farmed Deer. Topics have been chosen to elaborate areas of veterinary science of both national and international importance, and have been contributed by acknowledged experts in these fields. This review series has been sponsored by the Deer Branch of the New Zealand Veterinary Association, whose support is gratefully acknowledged. Our intention is to include a Feature Review Series in the December issue of the Journal each year. Ed.  相似文献   

A comparison of the socio-economic determinants of growth retardation in South African and Filipino infants     

Jones LL  Griffiths PL  Adair LS  Norris SA  Richter LM  Cameron N 《Public health nutrition》2008,11(12):1220-1228

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Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes     

Bou R  Guardiola F  Codony R  Faustman C  Elias RJ  Decker EA 《Journal of agricultural and food chemistry》2008,56(20):9612-9620

Myoglobin (Mb) and its iron have been proposed to be major prooxidants in cooked meats. To understand the mechanisms and differentiate between the prooxidant and antioxidant potential of oxymyoglobin (OxyMb) and metmyoglobin (MetMb), their prooxidant activity, iron content, solubility, free radical scavenging activity, and iron binding capacity were determined as a function of thermal processing. The ability of native and heat denatured OxyMb and MetMb to promote the oxidation of muscle microsomes was different. MetMb promoted lipid oxidation in both its native and denatured states. Conversely, OxyMb became antioxidative when the protein was heated to temperatures >or=75 degrees C. The increased antioxidant activity of heat denatured OxyMb was likely due to a decrease in its prooxidative activity due to its loss of solubility. These data show that the impact on oxidative reactions of Mb is the result of the balance between its antioxidant and prooxidant activities.  相似文献