Validation of new automated turbidimetric immunoassays for the measurement of haptoglobin and inter-α-trypsin inhibitor heavy chain H4 specific for the bovine species     

Anna Bassols  José Angel Robles-Guirado  Laura Arroyo  Lourdes Soler  Natalia García  Raquel Pato  Raquel Peña  Yolanda Saco  Ramon Armengol  Fermín Lampreave  María A. Alava  Francesca Canalias  Matilde Piñeiro 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(Z1):64-74

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穗肥施用时期对水稻籽粒中胚乳蛋白积累的影响   总被引：3，自引：1，他引：3  

丁艳锋  赵长华  王强盛  王绍华  黄丕生 《作物学报》2003,29(4):606-609

以武香粳9号为材料研究了不同穗肥施氮时期对水稻籽粒蛋白质积累的影响.结果表明:穗肥的施用提高了籽粒发育过程中单位干重蛋白质含量,以在倒0.5叶施用氮素穗肥影响最大;随施肥时期的延迟,稻米粗蛋白和谷蛋白含量逐步上升,醇溶蛋白含量逐渐降低;倒2.5叶施氮减少了强、弱势粒中蛋白组分的分布和含量,倒0.5叶施氮与此相反;胚乳  相似文献   

硬联接双电机的变频同步驱动方案研究   总被引：1，自引：0，他引：1  

刘希喆  吴捷  皮佑国  王洪勇 《中国农村水利水电》2003,(7):57-58,60

稳定性问题是硬联接双电机同步驱动系统要解决的关键。根据某拦河坝闸门控制系统的改造方案，论证了硬联接双电机采用转矩随动方式设计变频同步驱动方案的可行性。最终的调试实验结果表明，该系统具有较强的稳定性和优良的调速性能。  相似文献   

对缺硼反应不同的油菜基因型根系吸收硼特性的研究   总被引：5，自引：0，他引：5  

曹享云  刘武定 《华中农业大学学报》1997,16(5):335-339

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Natural Stable Isotopes for Determination of Gastrointestinal Transit Time in Fish          下载免费PDF全文

Lidiane Cristina Gonçalves de Sandre  Hellen Buzollo  Thiago Matias Torres do Nascimento  Lígia Maria Neira  Eduardo Gianini Abimorad  Rosangela Kiyoko Jomori  Carlos Ducatti  Maria Célia Portella  Dalton José Carneiro 《Journal of the World Aquaculture Society》2016,47(1):113-122

This study evaluated the application of stable isotopes of carbon as an alternative and more accurate method to determine gastrointestinal transit time (GTT) in fish by comparing it to the inert marker method. The stable isotope method detects alterations of the normal carbon flow in a biological system by analyzing naturally occurring isotopes of carbon, contrary to studies based on conventional techniques that apply external markers to the diet to determine GTT through visual observation of the color change in feces. Therefore, 320 pacu, Piaractus mesopotamicus juveniles were reared in 32 tanks under two different temperatures (25 and 29 C). The pacu juveniles received two different diets, one based on ingredients derived from C₃ photosynthetic cycle plants and the other based on C₄ plant ingredients, both containing titanium oxide (TiO₂) as a marker. After 40 d, the isotopic signature of the diets was changed, and the marker was replaced by chromic oxide (Cr₂O₃). In the isotopic technique, the feces were analyzed to determine the exchange in the isotopic ratio of carbon δ¹³C. Both methods found that GTT was faster (nearly 6 h) in fish at 29 C when using the C₄/C₃ feeding strategy and slower in fish at 25 C using the C₃/C₄ strategy (15 h by inert marker and 18 h by the isotopic method). In conclusion, GTT determination in pacu juveniles using the stable isotope technique exhibits the same accuracy obtained with the inert marker method at temperatures suitable (nearly 29 C) for the metabolism of these animals.  相似文献   

Detritivorous earthworms modify microbial community structure and accelerate plant residue decomposition     

María Gómez-Brandón  Cristina Lazcano  Marta Lores  Jorge Domínguez 《Applied soil ecology》2010,44(3):237-244

The impact of detritivorous earthworms (Eisenia andrei) on microbial community structure and function in grape marc, a lignocellulosic enriched plant residue, was investigated in a mesocosm experiment. Analysis of carbon and nitrogen pools was also carried out in order to evaluate how changes in microbial communities affect plant residue decomposition. The grape marc was completely processed after fifteen days as a result of the high density of earthworms present and the rapid gut transit time. Eisenia andrei had a large impact on the structure of the microbial community, as revealed by phospholipid fatty acid analysis. Earthworm activity reduced the abundance of both bacterial (except for Gram-negative bacteria) and fungal PLFA biomarkers relative to the control values. Decreases in microbial activity and in protease and cellulase activities were also attributable to the presence of earthworms. Moreover, earthworms strongly modified the substrate utilization patterns of microbial communities, as revealed by BIOLOG analysis. The presence of earthworms led to an increase in the utilization of some amino acids and polymers, which reached a higher substrate diversity value than that in the control mesocosm. The differences in microbial communities were accompanied by a reduction in the total C content and the labile C pool, relative to the control, although there were no significant differences in either cellulose or hemicellulose contents. However, total N content increased in both mesocosms – with and without earthworms – and the concentration of NH₄⁺ was also enhanced by earthworm activity. The results indicate that detritivorous earthworms play a key role in decomposing fresh plant residues in the short term via their intensive interactions with microbial communities.  相似文献   

新疆石竹亚科的分布系统及区系的研究     

潘晓玲  皮锡铭 《干旱区研究》1992,9(4):63-68

本文对新疆石竹亚科所含10属(共计77种,1变种)植物的分布、生境及系统位置作了详细的论述,并与新疆毗邻的哈萨克斯坦作了比较。通过研究指出新疆石竹亚科植物区系组成具有以下特征:(1)种类丰富;(2)属于中亚成分,其中以准噶尔—土兰成分和中亚—新疆—西西伯利亚成分占主要地位;(3)新疆石竹亚科植物区系与哈萨克斯坦近缘;(4)分化较强烈,特有成分丰富;(5)生态型组成复杂;(6)以旧世界温带分布属占主要地位;(7)南北疆分布种数悬殊;(8)单种属丰富。  相似文献   

粉锈宁药效与小麦品种对条锈菌感病性关系的研究     

陈扬林  陈万权  谢水仙  张淑香  杨立萍  柳红昕  胡箭卫  阎卫东  赵枢  杨丽  皮有松  杨业广 《植物保护学报》1992,19(1):75-80

在田间小区试验条件下,粉锈宁喷雾防治小麦条锈病,每666.6m~28克用药量对各类型感病小麦品种的效应是有显著差异的,对慢锈品种和前期中感,但抽穗后有抗性的品种均可控制为害,而对极感品种则未能控制产量损失;等比系列用药量对各类型感病品种的效应也是有明显差异的,在 ED_(50)和 ED_(95)(克/666.6m~2)平均值上,极感品种为9.38和74.85,高感品种为4.53和25.43,中感品种为3.62和20.96,慢锈品种为1.82和15.30。根据这一结果,模拟出各类型感病品种大田防治的每666.6m~2适宜用药量,高感品种9—12克,中感品种7—9克,慢锈品种4—6克,并经实践证明,这一剂量标准是可行的。拌种对压低各类型感病品种的成株期病情也有明显差异,造成这种差异的主要原因是各类型品种流行速度不同。  相似文献   

The effects of cyclophosphamide and its uroprotective agents, mesna and hyperbaric oxygen, on urinary bladder motility in guinea pigs     

Sağmanligil V  Etlik O  Pişkin I  Tomur A 《Acta veterinaria Hungarica》1999,47(4):451-460

The aim of this research was to observe the effects of cyclophosphamide and its uroprotective agents, mesna and hyperbaric oxygen (HBO), on the motility of urinary bladder muscle in guinea pigs. In the experimental groups, mesna and cyclophosphamide were intraperitoneally injected at a dose of 21.5 mg/kg and 68.1 mg/kg, respectively. For the combination of mesna and cyclophosphamide, one dose of mesna was injected 20 min before cyclophosphamide administration and three additional injections of mesna were repeated every three hours. A total of 8 HBO exposures were performed at 2.8 ATA for 90 min twice daily for another experimental group. In the HBO and cyclophosphamide combined group 5 HBO exposures were given prophylactically before cyclophosphamide. The combination of mesna, HBO and cyclophosphamide was administered by the same procedure. The contractions obtained in response to acetylcholine (ACh, 10(-4) M) in the control group were reduced using cyclophosphamide and HBO individually, but not by mesna. However, the contractions belonging to the various combinations of these three agents were not different from those seen in the control group. On the other hand, the combinations of cyclophosphamide, mesna and HBO showed higher responses to ACh than the groups in which cyclophosphamide and HBO were used individually, while the responses elicited by the cyclophosphamide and HBO combination were greater than those seen in the group treated with HBO only.  相似文献   

Virulence factors of avian pathogenic Escherichia coli isolated from broilers from the south of Brazil     

da Rocha AC  da Silva AB  de Brito AB  Moraes HL  Pontes AP  Cé MC  do Nascimento VP  Salle CT 《Avian diseases》2002,46(3):749-753

Sixty-three Escherichia coli strains isolated from broilers with respiratory problems were examined for virulence factors, hemolysin synthesis ability, motility, hemagglutination capacity, operon pap presence, colicin production, and serum resistance. The capacity to hemagglutinate guinea pig erythrocytes was found in 53 (84.1%) of the samples, but only 30 (47.6%) agglutinated chicken erythrocytes. D-mannose-sensitive hemagglutination against guinea pig erythrocytes was found in 19 (30.2%) samples and against chicken erythrocytes, in 15 (23.8%) samples, whereas the D-mannose-resistant hemagglutination with guinea pig erythrocytes was found in 34 (54%) samples, and 13 of these (20.6%) showed this characteristic against chicken erythrocytes. Operon pap, P fimbria codifier, was detected in 26 samples in a total of 34 D-mannose-resistant samples. Colicin production was observed in 55 (87.3%) of the strains, and 41.8% presented V colicin production. Of the samples analyzed, 56 (88.9%) presented serum resistance, six (9.5%) were intermediate, and only one (1.6%) was sensitive to the action of the complement. The diversity of virulence profiles detected in the samples in this study explains in part the multifactorial characteristics of avian colibacillosis.  相似文献