全文获取类型
收费全文 | 328篇 |
免费 | 9篇 |
专业分类
林业 | 28篇 |
农学 | 14篇 |
43篇 | |
综合类 | 9篇 |
农作物 | 10篇 |
水产渔业 | 59篇 |
畜牧兽医 | 147篇 |
园艺 | 2篇 |
植物保护 | 25篇 |
出版年
2023年 | 1篇 |
2022年 | 11篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 7篇 |
2017年 | 11篇 |
2016年 | 13篇 |
2015年 | 15篇 |
2014年 | 20篇 |
2013年 | 39篇 |
2012年 | 23篇 |
2011年 | 25篇 |
2010年 | 10篇 |
2009年 | 11篇 |
2008年 | 35篇 |
2007年 | 16篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 12篇 |
2003年 | 9篇 |
2002年 | 6篇 |
2001年 | 6篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1995年 | 2篇 |
排序方式: 共有337条查询结果,搜索用时 15 毫秒
271.
Mariko OKAMOTO Hirotaka FURUYA Ikuko SUGIMOTO Masahiro KUSUMOTO Daisuke TAKAMATSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):390
Paenibacillus larvae and Melissococcus plutonius are the causative agents of American and European foulbroods of honey bees, respectively. Since their virulence and resistance to disinfectants differ depending on the genotypes/phenotypes of the strains, the discrimination of strain types is important for the effective control of these diseases. Methods to detect and differentiate pathogens in honey are useful for surveying the contamination status of beehives/apiaries. In the present study, we selected a sequence (GenBank accession no. ) as the specific target for enterobacterial repetitive intergenic consensus (ERIC) II-type P. larvae strains for the first time and developed a novel multiplex PCR assay that precisely distinguishes between the major types of foulbrood pathogens (ERIC I and II P. larvae and typical and atypical M. plutonius) in one reaction. In addition, we found that commercially available kits designed for DNA extraction from Mycobacterium in feces efficiently extracted DNA from foulbrood pathogens in honey. Using the multiplex PCR assay and DNA extraction kits, all the targeted types of P. larvae and M. plutonius were detected in honey spiked with the pathogens at a concentration of 100 bacterial cells/strain/ml. Moreover, 94% of the Japanese honey samples examined in the present study were contaminated with one or more types of the foulbrood pathogens. These results indicate that the newly developed methods are useful for detecting foulbrood pathogens in honey. The epidemiological information obtained by these methods will contribute to the effective control of foulbroods in apiaries. FI763267相似文献
272.
273.
Kousuke Yatsuya Yukio Matsumoto Kei Sasaki Norio Shirafuji Daisuke Muraoka 《Fisheries Science》2017,83(6):939-946
The kelp Saccharina japonica is a dominant seaweed along the Sanriku coast where fisheries for sea urchin and abalone are among the most prosperous in Japan. We investigated the phenology of the kelp at depths of 6, 9 and 12 m from March 2015 to March 2016. The sea urchin Mesocentrotus nudus, a dominant herbivore off the coast, was also monitored with respect to its gonads for fishery use. Juvenile kelp appeared in March and rapidly elongated until May. Weight of the kelp significantly increased from April to July. Sori formation commenced from September and ceased in December. Density and biomass of the kelp were higher in the shallower sites than in the deeper one throughout the year. The highest biomasses recorded between July and October were 3773, 1939 and 501 g dry weight m?2 at depths of 6, 9 and 12 m, respectively. No regrowth of kelp blades was observed, indicating that S. japonica in this region has an annual life cycle. The gonad index of the sea urchin in the 12-m site was lower than that in the 6- and 9-m sites, indicating the close relation of the gonad index with the biomass of the kelp. 相似文献
274.
Daisuke Yamanaka Kazumi Sakuramoto Naoki Suzuki Tohru Nagasawa 《Fisheries Science》2007,73(4):837-844
Catch forecasting and the relationship between water temperature and catch in the snow crab Chionoecetes opilio in the western Sea of Japan were investigated. Catch was used as an index of abundance on the basis of high correlations
between catch per unit effort for the period when the latter data were available. The pattern of fluctuations, in catches
and index coincided well with each other. Therefore, catch data were regarded as an index of abundance and the correlation
coefficient between catch and water temperature was calculated at several depths from 1964 to 1999. Catch forecasting models
were composed using significantly correlated variables with the following results: (i) in April and September, the catch showed
high positive correlation with water temperatures (depth 50, 100, and 200 m, time lag 4–6 years); and (ii) a model using water
temperatures in April alone (depth 100 m, time lag 4–7 years) forecasted the catches with a coefficient of determination of
0.504, where models using more variables (water temperatures in the 2 months and catches) showed a coefficient of 0.587 at
most. Environmental conditions during the early life stages of the snow crab are thought to deeply influence the fluctuations,
in abundance. 相似文献
275.
Michiru Shiga Daisuke Shiode Satoshi Hayashi Tadashi Tokai Fuxiang Hu 《Fisheries Science》2008,74(3):479-487
ABSTRACT: A midwater float is a small float attached to the mainline of pelagic longlines to standardize the hook depth. In this study theoretical equations are presented for estimating the buoyancy of the midwater float required to lift the joints of the midwater float line on the mainline to the target depth. Sea trials using full scale tuna longline gear with midwater floats were carried out in the Indian Ocean in December 2004 and 2005, in order to examine the validity of the theoretical equations. In the sea trials, two types of midwater float settings, single midwater float setting and double midwater float setting, were tested and compared with the conventional setting. As a result, the joints of the midwater float line on the mainline were successfully lifted to the target depth as expected, demonstrating the validity of the theoretical equation. The range of hook depths in the midwater float setting was less spread over depths than in the conventional setting, and therefore, use of long float lines (100 m) with the midwater float setting allows all hooks to avoid entering the sea turtle habitat of shallower than 100 m depth. Factors affecting shoaling of the longline with the midwater float are also discussed. 相似文献
276.
Cuong Dinh Nguyen Shao-Hui Zheng Sachiyo Sanada-Morimura Masaya Matsumura Hideshi Yasui Daisuke Fujita 《Breeding Science》2021,71(5):497
Rice (Oryza sativa L.) yield is severely reduced by the brown planthopper (BPH), Nilaparvata lugens Stål, in Asian countries. Increasing resistance in rice against BPH can mitigate yield loss. Previous reports indicated the presence of three BPH resistance genes, BPH2, BPH17-ptb, and BPH32, in durable resistant indica rice cultivar ‘PTB33’. However, several important questions remain unclear; the genetic locations of BPH resistance genes on rice chromosomes and how these genes confer resistance, especially with relationship to three major categories of resistance mechanisms; antibiosis, antixenosis or tolerance. In this study, locations of BPH2, BPH17-ptb, and BPH32 were delimited using chromosome segment substitution lines derived from crosses between ‘Taichung 65’ and near-isogenic lines for BPH2 (BPH2-NIL), BPH17-ptb (BPH17-ptb-NIL), and BPH32 (BPH32-NIL). BPH2 was delimited as approximately 247.5 kbp between RM28449 and ID-161-2 on chromosome 12. BPH17-ptb and BPH32 were located between RM1305 and RM6156 on chromosome 4 and RM508 and RM19341 on chromosome 6, respectively. The antibiosis, antixenosis, and tolerance were estimated by several tests using BPH2-NIL, BPH17-ptb-NIL, and BPH32-NIL. BPH2 and BPH17-ptb showed resistance to antibiosis and antixenosis, while BPH17-ptb and BPH32 showed tolerance. These results contribute to the development of durable BPH resistance lines using three resistance genes from ‘PTB33’. 相似文献
277.
Eric Gilman Jeff Gearhart Blake Price Scott Eckert Henry Milliken John Wang Yonat Swimmer Daisuke Shiode Osamu Abe S. Hoyt Peckham Milani Chaloupka Martin Hall Jeff Mangel Joanna Alfaro-Shigueto Paul Dalzell & Asuka Ishizaki 《Fish and Fisheries》2010,11(1):57-88
There is growing evidence that small-scale, coastal, passive net fisheries may be the largest single threat to some sea turtle populations. We review assessments of turtle interactions in these fisheries, and experiments on gear-technology approaches (modifying gear designs, materials and fishing methods) to mitigate turtle by-catch, available from a small number of studies and fisheries. Additional assessments are needed to improve the limited understanding of the relative degree of risk coastal net fisheries pose to turtle populations, to prioritize limited conservation resources and identify suitable mitigation opportunities. Whether gear technology provides effective and commercially viable solutions, alone or in combination with other approaches, is not well-understood. Fishery-specific assessments and trials are needed, as differences between fisheries, including in gear designs; turtle and target species, sizes and abundance; socioeconomic context; and practicality affect efficacy and suitability of by-catch mitigation methods. Promising gear-technology approaches for gillnets and trammel nets include: increasing gear visibility to turtles but not target species, through illumination and line materials; reducing net vertical height; increasing tiedown length or eliminating tiedowns; incorporating shark-shaped silhouettes; and modifying float characteristics, the number of floats or eliminating floats. Promising gear-technology approaches for pound nets and other trap gear include: replacing mesh with ropes in the upper portion of leaders; incorporating a turtle releasing device into traps; modifying the shape of the trap roof to direct turtles towards the location of an escapement device; using an open trap; and incorporating a device to prevent sea turtle entrance into traps. 相似文献
278.
Kojima Hajime Kohgo Yuji Shimada Kiyoshi Shoda Daisuke Suzuki Hisato Saito Hirotaka 《Paddy and Water Environment》2020,18(2):431-442
Paddy and Water Environment - The 2011 Tohoku earthquake damaged many small earthen dams in Japan, causing flood damage and human casualties. Since then, the necessity for developing accurate... 相似文献
279.
Shunji Kurokawa Akito Kaga Mai Tsuda Daisuke Sekine Tomoko Shibuya 《Weed Biology and Management》2019,19(3):103-110
A canopy height‐to‐row spacing ratio (CHRSR) of 1.0 is expected to be a simple and practical onsite index for soybean growers to determine when further weed control is no longer necessary. This is decided when relative photosynthetic photon flux density (PPFD) reaches 50% under the soybean canopy. To evaluate the validity of this index, a 2‐year field experiment was conducted to compare days after sowing (DAS) to reach a relative PPFD of 50% (defined as PPFD50) and DAS to reach a CHRSR of 1.0 (defined as CHRSR1.0) in 190 accessions with different canopy architectures from a soybean core collection. A total of 104 accessions, for which final relative PPFD reached <10% in 2014, were examined for the differences between CHRSR1.0 and PPFD50. The mean and median values of the difference in 2014 were 0.02 and 2.2, respectively. Similarly, the mean and median values in 2015 were 2.8 and 2.6, respectively. Thus, CHRSR1.0 tended to be achieved slightly later than PPFD50, so a consideration was made that there would be a negligible risk of failure if growers used CHRSR1.0 to determine the termination time for weed control in the most of accessions. Although there were some accessions with a standing type of morphological characteristics or with a smaller or narrower leaf, showing negative differences between CHRSR1.0 and PPFD50 larger than 1 week, a CHRSR of 1.0 could be used to estimate PPFD50 in most of accessions without risk of failure to control weeds. 相似文献
280.
Megumi ITOH Yasuhiro NAKAJIMA Kouki KUWANO Daisuke MAEDA Yoshie SAKURAI Yoshitaka MATSUI Satoshi KAWAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):193
We previously reported the possibility of using the electrocardiogram variable to estimate blood calcium (Ca) concentration in dairy cows based on the strong positive correlation between the blood Ca concentration and the inverse of the corrected ST peak interval (STc−1). To improve the accuracy of the estimation of blood Ca concentration, we investigated the relationship between blood Ca concentration and STc−1 for each postpartum day and available variables other than STc−1. We measured multiple variables (milk yield, calving number, age, body temperature, etc.), including serum total Ca concentration (tCa), blood ionized Ca concentration (iCa) and STc−1 in 462 Holstein cows on days 0, 1, 2, 3, 5, and 7 postpartum. A very high correlation was observed between iCa and tCa. The association between tCa and STc−1 for each postpartum day had a high coefficient of determination of 0.61–0.79 postpartum 0–2 days but decreased after the third day. In the investigation using the data from postpartum days 0–2, STc−1, heart rate interval, calving number, and age were highly correlated with tCa. In addition, a multiple regression equation was obtained with tCa as the objective variable and STc−1 and calving number as explanatory variables. The estimation accuracy was improved as compared with the simple regression equation using only STc−1 as the explanatory variable. This multiple regression equation was used for 11 cows suspected of having hypocalcemia, and it was able to correctly detect cows requiring early treatment, except for one cow. 相似文献