CONTRAST AGENT Gd‐EOB‐DTPA (EOB·Primovist®) FOR LOW‐FIELD MAGNETIC RESONANCE IMAGING OF CANINE FOCAL LIVER LESIONS     

Yonetomi D  Kadosawa T  Miyoshi K  Nakao Y  Homma E  Hanazono K  Yamada E  Nakamura K  Ijiri A  Minegishi N  Maetani S  Hirayama K  Taniyama H  Nakade T 《Veterinary radiology & ultrasound》2012,53(4):371-380

Contrast‐enhanced magnetic resonance (MR) imaging with a new liver‐specific contrast agent gadolinium‐ethoxybenzyl‐diethylenetriamine penta‐acetic acid (Gd‐EOB‐DTPA; EOB·Primovist®) was studied in 14 normal beagles and 9 dogs with focal liver lesions. Gd‐EOB‐DTPA accumulates in normally functioning hepatocytes 20 min after injection. As with Gd‐DTPA, it is also possible to perform a dynamic multiphasic examination of the liver with Gd‐EOB‐DTPA, including an arterial phase and a portal venous phase. First, a reliable protocol was developed and the appropriate timings for the dynamic study and the parenchymal phase in normal dogs using Gd‐EOB‐DTPA were determined. Second, the patterns of these images were evaluated in patient dogs with hepatic masses. The optimal time of arterial imaging was from 15 s after injection, and the optimal time for portal venous imaging was from 40 s after injection. Meanwhile, the optimal time to observe changes during the hepatobiliary phase was from 20 min after injection. In patient dogs, 11 lesions were diagnosed as malignant tumors; all were hypointense to the surrounding normal liver parenchyma during the hepatobiliary phase. Even with a low‐field MR imaging unit, the sequences afforded images adequate to visualize the liver parenchyma and to detect tumors within an appropriate scan time. Contrast‐enhanced MR imaging with Gd‐EOB‐DTPA provides good demarcation on low‐field MR imaging for diagnosing canine focal liver lesions.  相似文献   

Genetic organization and preferential distribution of putative pilus gene clusters in Streptococcus suis     

Daisuke Takamatsu  Hiroto Nishino  Tomono Ishiji  Jun Ishii  Makoto Osaki  Nahuel Fittipaldi  Marcelo Gottschalk  Prasit Tharavichitkul  Shinji Takai  Tsutomu Sekizaki   《Veterinary microbiology》2009,138(1-2):132-139

Recent analyses of Streptococcus suis isolates using multilocus sequence typing (MLST) suggested the importance of sequence type (ST) 1 and ST27 complexes for animal hygiene and public health. In this study, to investigate whether pilus-associated genes in S. suis can be used as novel genetic markers for important clonal groups, we examined the correlation between STs and putative pilus-associated gene profiles in S. suis. Genomic searches using sequenced genomes and sequence data determined in several isolates revealed the presence of at least four distinct putative pilus gene clusters in S. suis (srtBCD, srtE, srtF, and srtG clusters). On the basis of the presence or absence of genes in the four clusters, 108 S. suis isolates from various origins were classified into 12 genotypes (genotypes A–L). Genotypes A and B, which possessed srtBCD plus srtF clusters and srtF plus srtG clusters, respectively, were the most common in isolates from diseased pigs and humans, and 29.9% and 59.8% of the isolates belonged to genotypes A and B, respectively. In contrast, only 4.8% and 28.6% of isolates from healthy carriers were genotypes A and B, respectively. MLST analysis showed the associations of genotypes A and B with ST1 and ST27 complexes, respectively. In addition, srtBCD and srtG clusters were preferentially distributed to ST1 and ST27 complex members, respectively. These results suggest that profiling of selected pilus-associated genes could be used as an easy screening method to monitor isolates important for S. suis infection.  相似文献   

Erratum to: Egg collection from hatchery-reared broodstock of spotted halibut Verasper variegatus treated with LHRH analog     

Daisuke Shimizu  Yuichiro Fujinami  Sayumi Sawaguchi  Takahiro Matsubara 《Fisheries Science》2012,78(6):1253-1253

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Inhibition of growth hormone secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes     

Sang-gun ROH  Yeon-hee HONG  Daisuke HISHIKAWA  Hiroaki TSUZUKI  Hisae MIYAHARA  Yukihiko NISHIMURA  Chizu GOTO  Yasuki SUZUKI  Ki-choon CHOI  Hong-gu LEE  Shinichi SASAKI 《Animal Science Journal》2005,76(4):381-386

Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH₂), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10^?7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6.  相似文献   

Malignant melanoma of the eyelid in a red squirrel (Sciurus vulgaris orientis)     

Fukui D  Bando G  Kosuge M  Yamaguchi M  Shibahara T  Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):261-264

A 6-year-old male red squirrel (Sciurus vulgaris orientis) developed bilateral tumors of upper and lower eyelids. The tumors in the left lid recurred despite surgical removal. Necropsy revealed metastasis to the lung. The neoplastic cells were epithelioid and highly pleomorphic, and only a few cells contained melanin granules. Occasionally melanoma cells were immunoreactive for S100, neuron-specific enolase and vimentin, and a small number of cells for cytokeratin. Ultrastructurally, the presence of premelanosomes was confirmed in the cytoplasm. Possible presence of cytokeratin-positive neoplastic melanocytes should be taken into account when differentiating a nonpigmented epithelioid melanoma from other tumors such as anaplastic carcinomas.  相似文献   

A meningioma with hyperintensity on T1-weighted images in a dog     

Hasegawa D  Kobayashi M  Fujita M  Uchida K  Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):615-617

A male six-year-old Pomeranian showed recurrent seizures and progressive left hemiparesis. MRI revealed a mass in the right paramedian frontal-temporal region with hyperintensity on T1-weighted (T1W) and mixed-intensity on T2-weighted (T2W) images. After gadolinium enhancement, the mass was enhanced homogenously and demonstrated the dural tail sign. Surgical resection of mass was performed and its histological diagnosis was meningioma. The meningioma of this case had a high cellular density with some lipid contents and intra- and extra-tumor hemorrhage, however, calcification was not found. These cellular characteristics may contribute to a higher signal intensity on T1W imaging.  相似文献   

The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus     

Yamane D  Kato K  Tohya Y  Akashi H 《Veterinary microbiology》2008,129(1-2):69-79

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Mapping of expressed sequence tag markers with a cDNA-amplified fragment length polymorphism method in Japanese quail (Coturnix japonica)     

Shinji SASAZAKI  Tomoko HINENOYA  Daisuke FUJIMA  Shinichi KIKUCHI  Akira FUJIWARA  Hideyuki MANNEN 《Animal Science Journal》2006,77(1):42-46

In our previous study, a Kobe‐NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA‐AFLP fragments and localized these markers on the linkage map. Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament‐deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.  相似文献   

Effect of environmental enrichment by providing trees as rubbing objects in grassland: Grazing cattle need tree-grooming     

Daisuke KOHARI  Takami KOSAKO  Michiru FUKASAWA  Hideharu TSUKADA 《Animal Science Journal》2007,78(4):413-416

We examined the role of trees as grooming objects in grazing pasture and investigated their necessity. Four grazing Japanese Black cows were used. Experimental pastures with restricted (RST) and released (RLS) grooming with trees were established. Examination was carried out for 24 h in each RST and RLS. The respective frequencies and duration of self‐grooming, allo‐grooming (social grooming) and grooming with trees was recorded by a continuous scan sampling method, and maintenance behavior was recorded by a 2‐min group animal scan sampling method. Grooming with trees in RLS occurred 29 ± 12 times/24 h and for 1342 ± 475 s/24 h. Total frequencies and duration of grooming in RLS tended to increase from that in RST (P = 0.08, P = 0.06, respectively). However, self‐grooming and allo‐grooming exhibited no differences. The results indicate that cattle may enrich their grooming through the use of trees if the latter are available. Furthermore, they may not substitute grooming with trees with other forms of grooming if no trees are available in the grassland. No difference was observed in the general proportion of their maintenance behavior. These results suggest that trees in grassland provide a good environmental enrichment object that satisfies cattle's potential needs of grooming and did not restrict their general behavior.  相似文献   

AN EVALUATION OF A NEW RADIOGRAPHIC TECHNIQUE UTILIZING A CONCAVE TABLE     

Yoshihiro  Kishigami  DVM  Arata  Yoshizaki  DVM  Daisuke  Saito  DVM  Hirokazu  Seo  DVM 《Veterinary radiology & ultrasound》2000,41(1):9-18

In conventional radiography systems, it is apparent that only the area immediately around the central x-ray beam can be evaluated accurately. Consequently in some instances, spinal radiography for example, several exposures are needed at various points along the body to create an accurate image for diagnosis. However, if the film and body part are in a concave shape such that the radius of the curve is equal to the film focal distance, the x-ray beam will penetrate the body and strike the film at two-dimensionally right angles in all areas. Using the spine as an example we found the curved technique had three major advantages over the traditional flat technique: lack of distortion, more uniform beam intensity due to a constant focal film distance, and improved resolution at the periphery of the radiograph because of lack of a cross over effect. It was concluded that an accurate evaluation of larger body parts can be made with minimal distortion utilizing the principles of a curved table technique.  相似文献