Microbiological Quality Assessment of Watershed Associated with Animal-Based Agriculture in Santa Catarina, Brazil     

Gilbert C. Sigua  Julio Cesar Pascale Palhares  Jalusa Deon Kich  Magda Regina Mulinari  Rosemari Martini Mattei  Jaqueline Bianca Klein  Susana Muller  Gustavo Plieske 《Water, air, and soil pollution》2010,210(1-4):307-316

Environmental problems many times could evolve when manure-containing pathogens are distributed into an open environment with no effort made to reduce the content of pathogens or limit their movement in the environment. Wind, surface flow, and subsurface flow can all carry enough pathogens to receiving waters to exceed water quality standards. This study was conducted to assess the microbiological quality of water associated with animal-based agriculture in the sub-basin of Pinhal River located in the rural area of Concordia, Santa Catarina, Brazil. Six sampling points representing different agricultural land uses (LU1—dairy cattle; LU2—without animals; LU3—dairy + pigs + poultry + crops; LU4—pigs + poultry + crops; LU5—dairy + pigs + poultry + crops + human; and LU6—dairy+pigs+crops) along the Pinhal River sub-basin (north to south) were sampled biweekly from August 2006 to December 2008. Concentrations of fecal coliforms and Escherichia coli varied significantly (p?≤?0.05) with land use (LU), but there was no interaction effect of LU, season, and time. Water samples from the catchment area of LU1 had the highest concentration of fecal coliforms (4,479?±?597 CFU ml^?1) when compared with other catchment areas. Catchment area associated with LU2 (no animal) had the lowest concentrations of fecal coliforms (39.2?±?5.2 CFU ml^?1). With the exception of LU2 (control site), all the maximum concentrations of E. coli exceeded the single maximum allowable concentration for E. coli (100 CFU ml^?1). When LU1 was compared with other catchment areas (LU3, 50%; LU4, 67%; LU5, 58%; and LU6, 44%), it had the lowest counts (39%) of Salmonella sp. Our results suggest that spatial pattern of bacterial water quality is evident, which can be linked to the different land uses and associated practices (present or absent of animal activities). Therefore, varying responses associated with the different land uses would be critical in identifying the importance of different sources of bacteria in the catchment area and the mechanisms transferring them.  相似文献   

Alternative matrices for cortisol measurement in fish     

Daniela Bertotto  Carlo Poltronieri  Elena Negrato  Duilio Majolini  Giuseppe Radaelli  Claudia Simontacchi 《Aquaculture Research》2010,41(8):1261-1267

Plasma cortisol is the most commonly used indicator of stress in fish but, as the blood sampling procedure itself can be a source of stress, it would be helpful to measure cortisol using less invasive matrices. It is also necessary to find alternative matrices as stress indicators in dead fish in which blood sampling is impossible. In the present study, we investigated transport stress in three aquaculture species, European sea bass (Dicentrarchus labrax L.), common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum), by cortisol determination (radioimmunoassay) in plasma and other matrices (skin mucus, gut content, lateral muscle and caudal fin). Cortisol significantly increased after transport in all species and matrices, except in the sea bass gut content, where it remained unchanged. The three species responded to transport stress by producing different cortisol levels. In conclusion, the significant correlation found between plasma cortisol and most of the other matrices opens up the possibility of using them to evaluate stress in fish: mucus sampling is a less invasive method than blood sampling, and in addition to muscle and fin sampling, it can be used in postmortem fish.  相似文献   

Effect of the nutrition level and sex on the energy maintenance requirement of broilers and rats     

M Klein  L Hoffmann  R Schiemann 《Archives of Animal Nutrition》1990,40(9):773-791

In 3 experiments young broiler chickens were supplied on various feeding levels in the growth range between 200 and 2500 g live weight (2.8 and 2.0 in experiment 1, 2.8, 2.3 and 1.8 in experiments 2 and 4, maintenance = feeding level 1.0). In experiments 1 and 2 short-time measurings (30 min) of heat production by means of gas exchange were made at 500, 1000, 1500 and 2000 g live weight in the post-absorptive state and on the maintenance level of feeding. In experiment 1 the heat production of the restrictively fed chickens was in 3 out of the 4 stages of development studied significantly lower by 10-14% than that of the intensively fed ones. In experiment 2 such an effect could not be observed. Fed on the level of energy maintenance, broilers weighing approximately 2000 g (experiment 3) did not show a significant influence of the sex on heat production as a result of 30-minute gas exchange measurings in the post-absorptive state. In experiment 4 24-hour measurings of the energy metabolism were carried out in the growth development in periods of feeding on the maintenance level by means of indirect calorimetry. In the live weight range between 0.9 and 1.3 kg there was only a low, non-significant decrease of the energy maintenance requirement of 4-6% of the highly restrictively fed chickens in comparison to the intensively fed ones. After continued feed restriction (live weight range 1.7-2.2 kg) an energy maintenance requirement reduced by 17% was measured. In an analogously arranged experiment with 24-hour energy metabolism measurings (experiment 5) male Wistar rats in the growth range between 70 and 280 g were supplied on the feeding levels 1.8 and 1.4 resp. In all three periods studied on the feeding level maintenance--beginning at 140-150 g live weight--the highly restrictively fed animals showed a slightly reduced energy maintenance requirement by 8%, 3% (non-significant) and 6% in comparison with the intensively fed animals.  相似文献   

Isolation and characterization of bone marrow-derived equine mesenchymal stem cells   总被引：4，自引：0，他引：4  

Arnhold SJ  Goletz I  Klein H  Stumpf G  Beluche LA  Rohde C  Addicks K  Litzke LF 《American journal of veterinary research》2007,68(10):1095-1105

OBJECTIVE: To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses. SAMPLE POPULATION: Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses. PROCEDURES: Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine selfrenewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transduction rate of equine MSCs. RESULTS: Equine MSCs can be culture-expanded. Equine MSCs undergo cryopreservation in liquid nitrogen without altering morphologic characteristics. Furthermore, equine MSCs maintain their ability to proliferate and differentiate after thawing. Immunocytochemically, the expression of the stem cell marker CD90 can be detected on equine MSCs. The multilineage differentiation potential of equine MSCs was revealed by their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation. CONCLUSIONS AND CLINICAL RELEVANCE: Our data indicate that bone marrow-derived stromal cells of horses can be characterized as MSCs. Equine MSCs have a high transduction rate and migratory potential and adapt to scaffold material in culture. As an autologous cell population, equine MSCs can be regarded as a promising cell population for tissue engineering in lesions of the musculoskeletal system in horses.  相似文献   

Inheritance of leafshape in turnip (Brassica rapa L. Partim) and rape (Brassica napus L.)     

Dick J. A. Klein Geltink 《Euphytica》1983,32(2):361-365

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QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry     

Elena?Zini  Franco?Biasioli  Flavia?Gasperi  Daniela?Mott  Eugenio?Aprea  Tilmann?D.?M?rk  Andrea?Patocchi  Cesare?Gessler  Matteo?KomjancEmail author 《Euphytica》2005,145(3):269-279

The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny ‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established. The two authors contributed equally to this work.  相似文献   

Sodium bicarbonate reduces postharvest decay development on melons   总被引：1，自引：0，他引：1  

Y. Aharoni  E. Fallik  A. Copel  M. Gil  S. Grinberg  J.D. Klein 《Postharvest Biology and Technology》1997,10(3):201-206

Sodium bicarbonate (SBC) inhibited in vitro mycelial growth of A. alternata, Fusarium spp. and R. stolonifer. SBC action was fungistatic rather than fungicidal. Coating commercially harvested ‘Galia’ and ‘Ein-Dor’ melons with wax containing 2% SBC reduced decay incidence after storage and shelf life simulation by four to seven-fold, to a commercially acceptable level of 6–7%, compared to untreated or waxed-treated controls. This treatment also maintained the fresh and blemish-free appearance of the fruit at harvest. Higher concentrations of SBC (3%) were phytotoxic and significantly reduced general fruit appearance. A trial shipment by sea transport to Europe demonstrated that 2% SBC incorporated into a wax coating maintained the marketability of ‘Galia’ melon fruits compared to that of untreated fruit. SBC can be an alternative biocide to the fungicide imazalil, thus eliminating unwanted residues on melon fruits.  相似文献   

Genetic variation among European maize inbreds for resistance to the European corn borer and relation to agronomic traits     

B. Schulz    R. Kreps    D. Klein    R. K. Gumber  A. E. Melchingeru 《Plant Breeding》1997,116(5):415-422

The univoltine European corn borer (ECB) has become a major limiting factor for maize (Zea mays L.) production in central Europe. The objective of this study was to survey the genetic variation for ECB resistance in European elite maize germplasm. Eighteen flint and 23 dent inbreds were screened under artificial ECB infestation at two locations in 1993 and 1994. Resistance was assessed by damage rating of broken plants, measurement of tunnel length in dissected stalks, and yield reduction in infested plots relative to insecticide-protected control plots. Flint lines showed significantly greater means for damage rating than dent lines with grain yield reduction of 35% and 24%, respectively. Significant genotypic variances among lines and high heritabilities were found for agronomic traits and damage rating. Heritabilities were intermediate for tunnel length and relative grain yield. Significant associations of days to silking, ear dry matter content, and dry matter yield of the whole plant with damage rating and tunnel length suggested a better resistance in late-maturing, high-yielding inbreds. Genotypic correlations of relative grain yield with tunnel length and damage rating ranged between ?0.46 and ?0.72. Partial correlations, eliminating the effect of flowering time, confirmed these associations. Damage rating of stalks is the most suitable trait for evaluation of ECB damage owing to its high heritability and easy recording. Tunnel length below the primary ear is a useful trait for assessing antibiosis because it is not correlated with days to silking. Inbreds with extreme resistance and susceptibility were identified which can be used as parents for establishing breeding and QTL mapping populations.  相似文献   

Interaction of the mutant genes B,og c,hp and t in the coloring of tomato fruit     

Thiago Matos Andrade  Wilson Roberto Maluf  Celso Mattes de Oliveira  Luiz Antônio Augusto Gomes  Daniela Costa Santos  Regis de Castro Carvalho  Ranoel José de Sousa Gonçalves  Álvaro Carlos Gonçalves Neto 《Euphytica》2015,205(3):773-783

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Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans     

Heimler D  Vignolini P  Dini MG  Romani A 《Journal of agricultural and food chemistry》2005,53(8):3053-3056

The antiradical activity of dry beans was evaluated in order to assess the validity of this test and to correlate the results with those achieved with the Folin-Ciocalteu method and with a rapid spectrophotometric method for the analysis of total flavonoids. Four landraces (12 samples) of common beans (Phaseolus vulgaris L.), collected in two regions of Italy (Tuscany and Basilicata) in three different years, were analyzed. The EC50 values ranged from 39 to 2810 mg sample/mg 1,1-diphenyl-2-picrylhydrazil radical. The phenolic content of each sample was expressed as gallic acid equivalents; it changed from 1.17 to 4.40 mg/g. The flavonoid content, expressed as mg of (+)-catechin per g of dry seeds, ranged from 0.24 to 1.43 mg/g. The qualiquantitative composition of polyphenols has been also elucidated by means of high-performance liquid chromatography (HPLC)-diode array detection and HPLC/MS. These investigations showed that rapid tests can contribute to assessing the quality of functional food.  相似文献