Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for polyomavirus DNA and antigen     

Kidney BA  Haines DM  Ellis JA  Burnham M  Jackson ML 《American journal of veterinary research》2001,62(6):828-832

OBJECTIVE: To determine whether vaccine site-associated sarcomas (VSS) from cats contain polyomavirus antigen or DNA. SAMPLE POPULATION: 50 formalin-fixed paraffin-embedded tissue blocks of VSS from cats. PROCEDURE: Sections from each tissue block were evaluated for polyomavirus antigen by use of an avidin-biotin-complex immunohistochemical staining method, using rabbit anti-murine polyomavirus polyclonal antiserum as the primary antibody. The DNA was extracted from sections of each tissue block, and a polymerase chain reaction assay was performed, using primers designed to amplify regions of the bovine polyomavirus genome and consensus polyomavirus primers designed to detect unknown polyomaviruses. RESULTS: Polyomavirus antigen and DNA were not detected in any of the VSS. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that polyomaviruses likely do not have any direct involvement in the pathogenesis of VSS in cats.  相似文献   

Immunohistochemical detection of tumor suppressor gene p53 protein in feline injection site-associated sarcomas     

Nambiar PR  Jackson ML  Ellis JA  Chelack BJ  Kidney BA  Haines DM 《Veterinary pathology》2001,38(2):236-238

Sarcomas associated with injection sites are a rare but important problem in cats. Immunohistochemical detection of p53 protein may correlate to mutation of the p53 tumor suppressor gene, a gene known to be important in oncogenesis. The expression of nuclear p53 protein in 40 feline injection site-assocated sarcomas was examined by immunohistochemical staining. In 42.5% (17/40), tumor cell nuclei were stained darkly; in 20% (8/40), tumor cell nuclei were stained palely; and in 37.5% (15/40), tumor cell nuclei were unstained. Immunohistochemical detection of p53 protein in a proportion of injection site-associated sarcomas suggests that mutation of the p53 gene may play a role in the pathogenesis of these tumors.  相似文献   

Sequential sampling for incidence of phomopsis leaf blight of strawberry     

Turechek WW  Ellis MA  Madden LV 《Phytopathology》2001,91(4):336-347

ABSTRACT Sequential sampling models for estimation and classification were developed for the incidence of strawberry leaflets infected by Phomopsis obscurans. Sampling protocols were based on a binary power law analysis of the spatial heterogeneity of Phomopsis leaf blight in commercial fields in Ohio. For sequential estimation, samples were collected until mean disease incidence could be estimated with a preselected coefficient of variation of the mean (C). For sequential classification, samples were collected until there was sufficient evidence to classify mean incidence as being below or above a threshold (p(t)) based on the sequential probability ratio test. Monte-Carlo simulations were used to determine the theoretical average sample number (ASN) and probability of classifying mean incidence as less than p(t) (operating characteristic) for any true value of incidence. Estimation and classification sampling models were both tested with bootstrap simulations of randomly selected data sets and validated by data sets from another year that were not utilized in developing the models. In general, achieved (or calculated) C after sequentially sampling for estimation was close to the preselected C of 0.2, and mean incidence was estimated with little bias. Achieving a C of 0.1 with less than 75 sampling units (the nominal value for many original data sets) was more problematical, especially with true incidence less than 0.2. ASN for classification was only 9 to 18 at disease incidence values near p(t), and approximately five or less at incidence values far from p(t). Correct classification decisions were made in over 88% of the validation data sets. Results indicated that it is possible to estimate Phomopsis leaf blight with high precision and with high correct classification probabilities.  相似文献   

USE OF COMPUTED TOMOGRAPHY AND RADIOLABELED LEUKOCYTES IN A CAT WITH PANCREATITIS     

Laurie L. Head  Gregory B. Daniel  Timothy J. Becker  David A. Lidbetter 《Veterinary radiology & ultrasound》2005,46(3):263-266

The normal feline pancreas has been evaluated using radiolabeled leukocytes (99mTc-HMPAO) and computed tomography. The purpose of this report is to describe a clinical case where both modalities were utilized to assess the inflamed feline pancreas. A nine year old female cat presented with anorexia, depression and some vomiting. Blood values were unremarkable. Radiographs and ultrasound were suggestive of pancreatitis. The cat's leukocytes were separated and labeled according to an established protocol. Whole body images were acquired immediately, at 5 and 30 min, and at 1, 2, 4, and 17 hours post injection. Approximately 48 h later, the animal was anesthetized and computed tomography of the abdomen was preformed both pre and post contrast. Surgical biopsies were taken. The distribution of the WBCs was similar to that documented in normal animals, however, at 2 h there was faint uptake seen in the region of the pancreas. This uptake became more intense at 4 h and persisted at 17 h. Computed tomography showed irregular margination of the pancreas, it was larger than normal and inhomogeneous. Contrast enhancement was inhomogeneous and its peak enhancement was not reached until 10 min post injection; normal feline pancreas enhances homogeneously and peaks immediately. Histopathology confirmed pancreatitis with lymphocytic, plasmacytic, neutrophilic and eosinophilic inflammation and fibrosis. Radiolabeled leukocytes can be used to document pancreatic inflammation and this is best seen 4 h after injection. Computed tomography allows superior visualization of the pancreas. Both the appearance and contrast enhancement pattern of the inflamed pancreas differ from normal.  相似文献   

Introduction to veterinary physical rehabilitation.     

David Levine  Darryl L Millis  Denis J Marcellin-Little 《Veterinary Clinics of North America: Small Animal Practice》2005,35(6):1247-54, vii

Physical therapy is a profession with an established scientific basis in human beings and companion animals. It has a large number of clinical applications in the restoration, maintenance, and promotion of optimal physical function. In providing physical therapy, the goal is to restore, maintain, and promote optimal function, optimal fitness, wellness, and quality of life as they relate to movement disorders and health. A major emphasis is to prevent or minimize the onset, clinical signs, and progression of impairments, functional limitations, and disabilities that may result from diseases, disorders, conditions, and injuries.  相似文献   

Physical agent modalities.     

Janet E Steiss  David Levine 《Veterinary Clinics of North America: Small Animal Practice》2005,35(6):1317-33, viii

The purpose of this article is to review the use of cold, heat, therapeutic ultrasound, and electrical stimulation in small animal rehabilitation.The material in this article is a compilation from the veterinary and hu-man literature. Additional information is needed on how to adapt the techniques used in human beings to small animals and then to establish the efficacy of these techniques in animals.  相似文献   

Rehabilitation and conditioning of sporting dogs.     

Denis J Marcellin-Little  David Levine  Robert Taylor 《Veterinary Clinics of North America: Small Animal Practice》2005,35(6):1427-39, ix

Owners and trainers exercise sporting dogs to increase their fitness and optimize their conditioning and performance. Training is designed to in-crease strength, endurance, and agility and is sport-specific. Sporting dogs are susceptible to specific musculoskeletal injuries. The rehabilitation of sporting dogs after these injuries follows specific principles during the acute, subacute, and reconditioning periods.  相似文献   

Equine herpesvirus-4 kinetics in peripheral blood leukocytes and nasopharyngeal secretions in foals using quantitative real-time TaqMan PCR.     

Nicola Pusterla  Christian M Leutenegger  W David Wilson  Johanna L Watson  Gregory L Ferraro  John E Madigan 《Journal of veterinary diagnostic investigation》2005,17(6):578-581

  相似文献   

Reviews section     

Dr Bill Symondson  W. O. C. Symondson  Dr Peter McEwen  Simon Oxley  Dr Patthumal Beevi  Houston Hobbs  Keith Harris  Dr R. T. Plumb  Professor P. G. Mantle  Professor J. Hemingway  Dr Barbara B. Shew  R. M. Baker  Professor P. A. Langley  David Dent 《Integrated Pest Management Reviews》1996,1(2):111-123

  相似文献   

Effects of some nonionic polyoxyethylene surfactants on uptake of ethirimol and diclobutrazol from suspension formulations applied to wheat leaves     

Peter J. Holloway  Wilson W.-C. Wong  Hazel J. Partridge  David Seaman  Richard B. Perry 《Pest management science》1992,34(2):109-118

The influence of a number of commercial nonionic polyoxyethylene surfactants on the foliar penetration and movement of two systemic fungicides, ethirimol and diclobutrazol, was studied in outdoor-grown wheat plants at different growth stages and post-treatment temperatures in two consecutive growing seasons. Both fungicides were applied as ca 0·2 μl droplets of aqueous suspension formulations containing 0·5 g litre^?1 of ¹⁴C-labelled active ingredient; surfactants were added to these suspensions at concentrations ranging from 0·2-10 g litre^?1. To achieve optimum uptake of each fungicide the use of surfactants with different physicochemical properties was required. For diclobutrazol, a lipophilic compound, uptake of radiolabel was best with surfactants of low mean molar ethylene oxide (E) content (5-6) but it was necessary to use concentrations of ca 5 g litre^?1 to attain this. The surfactant threshold concentration for uptake enhancement of radiolabel from ethirimol formulations (< 2 g litre^?1) was much lower than that for diclobutrazol but surfactants with E contents > 10 induced the greatest amount of uptake. For both fungicides, surfactants with an aliphatic alcohol hydrophobe were generally more efficient in promoting their uptake than those with a nonylphenol moiety. The sorbitan-based surfactant ‘Tween 20’ proved to be an effective adjuvant only for the ethirimol formulation; the uptake enhancing properties of the block copolymer ‘Synperonic PE/F68’ were weak. Uptake performance could not be related to the spreading properties of the respective formulations on the wheat leaf surface or to differences in solubilisation of the two fungicides by the surfactants. Although surfactants could substantially increase the amount of acropetal transport of radiolabel from both fungicides, none of those tested specifically promoted it; a constant proportion of the radioactive dose absorbed by a treated leaf was usually exported away from the site of application. The results are discussed in the light of current theories about the mode of action of surfactants as spray adjuvants.  相似文献