不同保存方法对光皮桦总DNA提取效果的影响   总被引：10，自引：0，他引：10  

李志真  谢一青  黄儒珠  黄勇  陈杰 《分子植物育种》2006,4(1):131-134

光皮桦是一种顽拗类植物,其鲜叶组织中富含酚类、糖类及其他次生代谢物。这些物质很容易使鲜叶发生褐变,从而严重影响植物基因组DNA的提取质量。本文以新鲜嫩叶为对照,比较了-20℃冷藏、硅胶干燥、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液固定4种鲜叶保存方法对提取光皮桦基因组总DNA效果的影响,寻找最佳的光皮桦嫩叶保存方法。并以提取的核酸得率、纯度、片段分布情况、是否含有PCR反应抑制剂等指标来评价其保存效果。结果表明:-20℃冷藏的样品未能提到DNA;新鲜样品、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液保存的样品均提到纯度较好的DNA,大小在48Kb左右,得率为400!g/g鲜叶左右,并获得条带清晰、多态性好的PCR扩增图谱;用硅胶干燥保存的样品也能提到DNA,但得率低,为51.2!g/g鲜叶,且未获得条带完整、清晰的PCR扩增图谱。  相似文献   

小麦品种贵农21,贵农22抗病性研究   总被引：1，自引：0，他引：1  

王凤乐  吴立人 《种子》1992,(5):16-18

采用温室苗期测定和成株期人工诱发接种鉴定方法研究了簇毛麦——硬粒小麦杂种后代贵农21、22的抗病性。苗期抗性测定表明,贵农21、22对国外15个小麦条锈菌生理小种表现为免疫至近免疫反应,仅对具有毒性基因Yr10的菌系82E16表现出中度感病,对供试的国内14个主要条锈菌菌系呈观免疫至近免疫反应。田间成株期抗性试验结果显示,贵农21、22对小麦条锈病、白粉病免疫,高抗秆锈病,中抗叶锈病。小麦品种贵农21、22可作为抗病资源和生产品种加以推广应用。  相似文献   

A Mininum Ring-band Mathematical Definition, Numerical Algorithm     

Xie Ming Guo Xiaoyu Wang Yanchun 《保鲜与加工》1993,(6):134-137

A minimum ring-band mathematical definition :In the done ring-band, there are at least two inner points and two outer points which scatter staggeringly on the circumference. With this definition as the rule of searching calculation' s ending,a fast numerical algorithm of gaining the minimum ring-band of a closed curve was suggested:Make a ring-band with the four points scattered staggeringly,then replace the old points with new ones continuously until all the points are located within the ring-band. The method was used in an application example to measure the spindle rotating precision of machine tools.  相似文献   

NaHSO3对惠水金钱橘叶片光合速率的影响     

张明生  王素英  臧红兵  殷召静  谢波  杨永华 《种子》2004,23(6):21-23

用不同浓度的NaHSO3及其与KCl和6-BA配合对贵州惠水金钱橘叶表进行喷施,研究其对叶片光合作用的影响.结果表明:较低浓度(3～9mmol/L)的NaHSO3对叶片光合速率有明显促进作用,作用效果长达6d之久;当NaHSO3浓度较高(≥12mmol/L)时对光合作用不利;低浓度的NaHSO3分别与1mmol/L KCl和5mmol/L 6-BA配合施用,对叶片光合速率的促进效果更显著.  相似文献   

盾叶薯蓣良种离体快繁关键技术研究   总被引：7，自引：0，他引：7  

刘贵周  谢世清 《中国种业》2005,(6):36-37

以盾叶薯蓣的单节茎段为外植体,培养基 MS 2.0mg/L BA 0.6mg/L NAA对腋芽诱导效果最好,诱导率达 77.4%; MS 0.5mg/L BA 0.5～ 1.0mg/L 2,4- D为诱导愈伤组织的培养基;在 MS 1.0mg/L BA 0.2mg/L NAA或 MS 2.0mg/L BA 0.5mg/L IBA培养基上能有效地诱导多芽; BA与 NAA的组合较 BA与 IBA组合更有利于多芽的诱导; MS 2.5mg/L BA 0.5mg/L NAA可作为愈伤组织及多芽组织的继代培养.  相似文献   

EXPERIMENTAL INVESTIGATION ON VIBRATION AND NOISE BY C6132 LATHE     

Wang Yanchun Ding Kang Xie MingHuang Qiang Yi Zhulin 《保鲜与加工》1992,(5):1-7

This paper presents the principle of high-pass-absolute-value demodulation tcchnigue which is applied with spectrum analysis to investigate the vibration and noise produced by the C61 32 Lathe. The main noise sourse is found out and some reliable data are provided to decrease the noise and vibration and to improve the dynamic performence of the c6132 Lathe.  相似文献   

A METHOD FOR MEASUREING LARGE OPTICAL PATH DIFFERENCE WITH THE HOLOGRAPHIC THREE WAVEFRONT INTERFEROMETER     

Wen Guangjun Xie Fuzhen Wang Ding 《保鲜与加工》1992,(1):134-138

The holographic three wavefront interferometer is used to measure large optical path difference. This method is proved to be practical through the measurement of the deformation -Voltage Curve of piezocrystal.  相似文献   

Research on Dynamics of Installation Way for Active Isolation Actuator on Flexible Foundations     

Gu Qiankun Tang Yike Yu Youju Xie Zhijiang Liang Xichang 《保鲜与加工》1997,(6):96-102

The dynamic characteristic of active isolation atuator on flexible foundations was studied under the installation of parallel structure and foundation cancellation.The influencing relationships between active isolation force of the different installation ways and passive isolation system and flexible foundation were analyzed and compared.Scientific basis for selecting installation ways of active isolator is provided in this paper.  相似文献   

ELLIPSOMETRIC SPECTROSCOPY OF ELECTROCHROMISM OF POLYANILINE FILMS     

Xie Shangfen Shen Hui 《保鲜与加工》1991,(4):93-96

The electrochromism of polyaniline films, which is prepared by electropolymer-ization from aniline in acidic aqueous solution onto platinum,is investigated in 330-700nm through in-situ ellipsometric technique. The results indicate that the ellipsometric peak of the reduced state(-0. 2V) of the film appears at 390-400nm and the oxidized state(0. 55V) at 500-530nm. The elec-trochromic reaction from the reduced "yellow" state to the oxidized "green" state is suggested via intermediate state whose ellipsometric peak appears at 466-470nm.  相似文献   

Study on the Land Use Right in Urban Housing Dismantling and Moving     

Ceng WenGe  Yang YuJing  Xie FuCheng 《保鲜与加工》2005,(3):125-129

With the strengthening of constitutional protection that legal private property is not to be encroached upon and the land usufructs paid regime are further clear, the regime of urban housing dismantling and moving is facing a vigorous test. This paper analyzes three problems: the justice of the withdrawal of land use right, the legality of the alteration of the land use right and the rationality of the compensation of the land use right during the urban housing dismantling and moving. Finally, this paper tries to find some effective measures to solve the chain problems.  相似文献