Cyanophora paradoxa genome elucidates origin of photosynthesis in algae and plants     

Price DC  Chan CX  Yoon HS  Yang EC  Qiu H  Weber AP  Schwacke R  Gross J  Blouin NA  Lane C  Reyes-Prieto A  Durnford DG  Neilson JA  Lang BF  Burger G  Steiner JM  Löffelhardt W  Meuser JE  Posewitz MC  Ball S  Arias MC  Henrissat B  Coutinho PM  Rensing SA  Symeonidi A  Doddapaneni H  Green BR  Rajah VD  Boore J  Bhattacharya D 《Science (New York, N.Y.)》2012,335(6070):843-847

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<Emphasis Type="Italic">Echinococcus granulosus</Emphasis> of Buffalo in India: Partial Characterization of Excretory-Secretory and Germinal Membrane Antigens     

Maity A  Bhattacharya D  Batabyal S  Chattopadhyay S  Bera AK  Karmakar PK 《Veterinary research communications》2007,31(4):457-460

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Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal,India     

Ramesh Chandra Malick  Asit Kumar Bera  Hemanta Chowdhury  Manojit Bhattacharya  Tanuja Abdulla  Himanshu Sekhar Swain  Raju Baitha  Vikas Kumar  Basanta Kumar Das 《Aquaculture Research》2020,51(6):2410-2420

The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase‐negative, nitrate‐negative, catalase‐positive and indole‐negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β‐lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD₅₀ of 1.24 × 10⁵ and 1.88 × 10⁷ cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug‐resistant (MDR)‐A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland.  相似文献   

Breeding systems and seed production for six weedy taxa of Bidens          下载免费PDF全文

Jian‐Hua Hao  Saurav Bhattacharya  Liang Ma  Li‐Xin Wang 《Weed Biology and Management》2018,18(1):41-49

Successful reproduction is considered to be the major determining factor for a weedy population to establish, colonize and infest. The breeding systems and seed production of six weedy taxa of Bidens were studied at an experimental garden in Jiangsu province in China. The results from four treatments (open pollination, bagged capitulum, decapitated capitulum, and pollen supplement) indicated that, with the exception of Bidens pilosa var. radiata, all the other five Bidens taxa; namely, B. pilosa var. pilosa, B. pilosa var. minor, B. frondosa, B.bipinnata, and B. biternata, were self‐fertile with a high seed‐set in bagged capitula, and a high autofertility index. Including the trait of vegetative propagation in self‐incompatible B. pilosa var. radiata, all the six weedy taxa of Bidens had the capacity of uniparental reproduction. The pollen limitation values for all six Bidens taxa were low in the present study. However, the seed production of B. pilosa var. radiata is likely to decrease in the absence of sufficient pollinators, related to its high pollen contribution and potential pollen limitation indices. The seed production per plant and per capitulum of the three varieties of B. pilosa was the highest among the six taxa and might contribute to their serious weediness. The breeding system type and seed number per capitulum could be used as a classification reference for Bidens taxa.  相似文献   

Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine   总被引：1，自引：1，他引：0  

Bandyopadhyay S  Sasmal D  Dutta TK  Ghosh MK  Sarkar M  Sasmal NK  Bhattacharya M 《Tropical animal health and production》2009,41(4):587-592

The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication programme of brucellosis in yaks.  相似文献   

Influence of synthetic plant growth substances on the survivorship and developmental parameters of <Emphasis Type="Italic">Spilarctia obliqua</Emphasis> Walker (Lepidoptera: Arctiidae)     

G. Gupta  S. R. Yadav  A. K. Bhattacharya 《Journal of pest science》2009,82(1):41-46

The nontarget effects of three plant growth stimulants (gibberellic acid, Siapton and triacontanol) and two plant growth retardants (chlormequat chloride and mepiquat chloride) on the survivorship and developmental parameters of Spilarctia obliqua Walker (Lepidoptera: Arctiidae) were studied. For each compound, various concentrations (0.125×, 0.25×, 0.5×, × , 2× and 4×; × = labeled dose) bracketing the labeled dose were prepared by serial dilution and mixed with an artificial diet for the insect. Gibberellic acid caused an increase in the larval period at × − 4×, but no marked difference was observed in the pupal period or in larval survival at any concentration. Siapton induced a significant increase in larval period at the two highest doses but caused significant reduction in pest survival at × − 4×. Interestingly, triacontanol induced a significant reduction in pest survivorship at almost all doses. The LC₅₀ for triacontanol was 0.206% and LT₅₀ was 266.5 hours at the labeled dose. However, triacontanol did not cause any significant difference in larval or pupal periods at any dose tested. Chlormequat chloride and mepiquat chloride caused significant reduction of larval survivorship at concentrations higher than the labeled doses, and no effect on the larval period. Intriguingly, a significant reduction in pupal period was observed with chlormequat chloride at × and 0.25×. Based on the marked effect of triacontanol on pest survivorship, this study suggests incorporating triacontanol in integrated pest management modules for S. obliqua. Caution in the use of gibberellic acid and Siapton for pest infestations is also indicated.  相似文献   

Role of exogenous phytohormones on growth and plumbagin accumulation in Plumbago indica hairy roots and conservation of elite root clones via synthetic seeds     

Moumita Gangopadhyay  Saikat DewanjeeDipjyoti Chakraborty  Sabita Bhattacharya 《Industrial Crops and Products》2011,33(2):445-450

The present study describes the role of different exogenous hormones on morphology and plumbagin production in Plumbago indica hairy roots. It was also aimed to conserve elite root clones via synthetic seed technology. Insertion of rolB gene in transformed roots was confirmed by polymerase chain reaction followed by southern blot analysis. Hairy roots were treated with single or in combination of different phytohormones viz. IAA, IBA, 2, 4-D, NAA, BAP, GA₃ and ABA. Cultures incubated with GA₃ (0.5 mg l⁻¹) yielded highest root growth due to formation of profuse lateral branching while NAA (0.5 mg l⁻¹) treatment caused highest plumbagin accumulation. Cultures incubated with 2, 4-D exhibited highest inhibitory effect in terms of both root growth and plumbagin production. All phytohormones were found to be effective at lower concentration. In combinatorial study, GA₃ + NAA (0.5 mg l⁻¹, each) was found optimum for root biomass and plumbagin production at earlier stage of culture. Different combinations of auxins and BAP induced different morphologies ranging from reduction of lateral branching to rapid disorganization of root matrix. The combinations of ABA and selected auxins were not found promising at any of selected concentration. Based on the effect of exogenous hormones on hairy root culture, elite root clones were selected and encapsulated with sodium alginate matrix. Uniform shaped alginate coated synthetic seeds were conserved up to 6 months exhibited high regeneration potential without disturbing plumbagin content.  相似文献   

Economic analysis of risk of gastrointestinal parasitic infection in cattle in North Eastern States of India     

Bandyopadhyay  S.  Mandal  S.  Datta  K. K.  Devi  P.  De  S.  Bera  A. K.  Bhattacharya  D. 《Tropical animal health and production》2010,42(7):1481-1486

Gastrointestinal parasitic infection is highly prevalent in the North Eastern States (NEH) of India and accounted for significant economic losses across various livestock species. Productivity of cattle in terms of milk yield was estimated to be considerably higher (3,715, 3,590, and 3,154 L) due to strategic anthelmintic treatment as compare to control group (2,928 L). Based on the probability of occurrence of parasitic infection as well as increase in value of milk production, the possible economic gain at state level has been estimated to be Rs. 46 million, Rs. 35 million, and Rs. 14 million, depending upon the different strategic treatment. The government may take up the program to educate the cattle farmers on strategic management against parasitic infecion and simultaneously making available various anthelmintic medicines. This public responsibility of the government to minimize the risk and economic loss due to gastrointestinal parasite infection may reduce the private cost and thereby would increase the social benefits in North Eastern states of India.  相似文献   

Somatic embryogenesis in Cymbopogon pendulus and evaluation of clonal fidelity of regenerants using ISSR marker     

S. Bhattacharya  T.K. Bandopadhyay  P.D. Ghosh 《Scientia Horticulturae》2010

An efficient plant propagation system through somatic embryogenesis was established in Cymbopogon pendulus, an aromatic grass followed by analysis of genetic status of regenerants using ISSR markers. Optimum embryogenic callus induction was observed on MS basal medium supplemented with 13.57 μM 2,4-dicholorophenoxyacetic acid (2,4-D) with 8.88 μM N⁶-benzyladenine (BA). Subsequent culturing of embryogenic calli on MS medium containing 4.52 μM 2,4-D and 8.88–13.32 μM BA gave maximum number of somatic embryos. Addition of coconut water (CW) promoted induction, growth and differentiation of callus and somatic embryogenesis. Further development of embryos into plantlets was achieved on MS medium supplemented with lower concentration of biotin and calcium pantothenate (CaP) along with BA (4.44–13.32 μM) and kinetin (2.32–4.65 μM). The root meristems were established on half strength MS medium containing 2% sucrose and 2.46–9.84 μM Indole3-butyric acid (IBA) and successfully established in soil with 77.8% survival rate in field condition. Thirteen randomly selected regenerated clones were screened using six ISSR primers. Nine clones produced similar monomorphic amplification profiles while remaining clones showed minor variation with absence of certain parental bands and appearance of unique band. Majority of the regenerants maintained genetic fidelity with the generation of few variants as evidenced from similarity matrix estimates using Nei Li's coefficient of similarity data.  相似文献   

The disposition kinetics and residues of fenvalerate in tissues following a single dermal application to black bengal goats     

T. K. Mandal  A. K. Chakraborty  A. Bhattacharya  R. K. Ghosh  S. Majumder 《Veterinary research communications》1996,20(3):265-272

The disposition kinetics of fenvalerate were studied in goats after dermal application of 100 ml of 0.25% (w/v) solution. The insecticide persisted in the blood for 72 h. The mean (±SEM) V _d(area) and apparent t _1/2 () were 9.92±1.44 L/kg and 17.51±2.65 h, while the AUC and Cl_B values were respectively 82.15±7.40 g h/ml and 0.56±0.05 L/(kg h). Four days after the dermal application, the highest concentration of fenvalerate residues was found in the adrenal gland, followed by the biceps muscle, omental fat, liver, kidney, lung and cerebrum in that order. Fenvalerate caused hyperglycaemia but had no effect on serum protein and cholesterol levels. Serum acetylcholinesterase activities were increased after 24 h but were below the initial values from 48 to 120 h.Abbreviations Ache acetylcholinestase - AUC total area under the blood insecticide concentration-versus-time curve - Cl_B total body clearance - GLC gas-liquid chromatography - t _1/2() apparent elimination half-life - V _d(area) apparent volume of insecticide distribution based on area method  相似文献