During the present investigation a total of forty Indian animal isolates were screened by single strand conformation polymorphism (SSCP) collected from sheep, goat, cattle and buffalo. The result of the study indicated that nuclear variants of Echinococcus granulosus were present in both small and large ruminants. SSCP phenotypes of AgB, intron of actin II and Hbx-2 have been deduced. Presence of nuclear variants due to mutation of E. granulosus has been discussed depending on hypotheses imparted earlier in literature. High polymophism of AgB demands further investigation because the gene is related with immune evasion and infectivity. This communication reports for the first time the comparative profile of Indian goat, sheep, cattle and buffalo isolates of E. granulosus complex. 相似文献
SALTMOD is a simulation model whichpredicts root zone soil salinity, drainagewater quality and water table depth inagricultural land under differentgeo-hydrological conditions and varyingwater management scenarios. The model wasapplied to the data from coastal AndhraPradesh of India where subsurface drainagesystem is laid out at several drainspacings at the experimental site. Fielddata for 1999, 2000 and 2001 were collectedfrom 35 and 55 m drain spacing plots forSALTMOD application. Modelling was doneconsidering two simulation approaches. Thefirst approach (Simulation-I) used the sameinitial values for the entire simulationperiod. In the second approach(Simulation-II), the computations wereperformed year-by-year, giving each yearthe current input values obtained from thesimulation results of the previous year.Results of these two approaches weredifferent from each other. Simulation-IIgave better predictions than that ofSimulation-I in terms of closeness to theobserved values. Simulation results ofsoil salinity in the root zone, drainagewater quality and quantity and depth towater table revealed that the salinity ofroot zone was predicted more accuratelythan that of drainage water quality anddepth to water table. Also throughsimulation, it was found that the salinityof drainage water was relativelyindependent of the root zone soil salinity. Model application study suggests thatSALTMOD can be used with confidence toevaluate various drain spacings of asubsurface drainage system and facilitatereasonable prediction of the reclamationperiod. 相似文献
Fixation of Zn and Cu applied to tropical rice-growing lateritic soils rich in Fe-oxides may be reduced if the soils are
kept flooded for a few days before their application. There may be a further reduction if such flooding is combined with incorporation
of green manures. To investigate this effect, a laboratory experiment was conducted to study the effect of different periods
(0 and 15 days) of preflooding combined with (0 and 0.50% of soil weight) Sesbania rostrata and Azolla microphylla incorporation as green manures on the transformation of applied Zn and Cu in two lateritic rice-growing soils. Recovery of
added Zn/Cu in DTPA (diethylene triamine pentaacetate)-extractable form was always found to be higher when they were applied
after the soils were maintained in a flooded state for 15 days than when applied immediately after flooding; this effect was
more prominent in respect of Cu. Contrary to expectations, green manure incorporation along with preflooding caused a significant
decrease in recovery of Zn/Cu; the effect, however, showed a decreasing trend as incubation progressed. The effect was more
marked with A. microphylla than with S. rostrata, particularly with Cu. Possible causes of such changes and their implications on the Zn/Cu nutrition of rice are discussed.
Received: 7 August 1995 相似文献
Tropical Animal Health and Production - Brucellosis is an important zoonosis worldwide. In livestock, it frequently causes chronic disease with reproductive failures that contribute to production... 相似文献
1. Single strand conformation polymorphism analysis and DNA sequencing was performed in White Leghorn hens to explore the polymorphisms present in the promoter of the prolactin gene. The effects of different genotypes on egg production and quality traits were determined, and expression of the prolactin gene in different genotypes was quantified by real time-PCR.
2. Five genotypes and four alleles at each of two Fragments of the promoter were found, of which the FG genotype in Fragment 1 and the PQ genotype in Fragment 2 were the most predominant genotypes.
3. The genotypes of Fragment 1 had significant effects (P?0·05) on Haugh unit, albumen weight, albumen percentage and shell percentage at 40 weeks of age; egg weight and yolk index at 52 weeks of age; and egg weight at 64 weeks of age.
4. Prolactin expression in the genotypes of Fragment 1 differed significantly and GH genotyped birds had the highest level of expression. The genotypes of Fragment 2 did not show any significant differences of expression.
5. It was concluded that the prolactin gene promoter was highly polymorphic, and had significant effects on egg quality traits in White Leghorn hens. 相似文献
Objective: To define the kinematic motion patterns of the canine cervical spine, with a particular emphasis on identifying differences between the cranial (C2–C4) and caudal (C5–C7) segments, and to determine the significance of coupled motions (CM) in the canine cervical spine. Study Design: Cadaveric biomechanical study. Sample Population: Cervical spines of 8 Foxhounds. Methods: Spinal specimens were considered free of pathology based on radiographic, computed tomography, and magnetic resonance imaging examinations. All musculature was removed without damaging ligaments or joint capsules. Spines were mounted in a customized pure‐moment spine testing jig, and data were collected using an optoelectronic motion capture system. Range of motion, neutral zone and CM in flexion/extension, left/right lateral bending and left/right axial rotation were established. Data were analyzed using mixed‐effects maximum likelihood regression models. Results: Total flexion/extension did not change across the 4 levels. There was no difference between flexion and extension, and no CM was identified. Lateral bending was not different across levels, but tended to be greater in the cranial spine. Axial rotation was ~2.6 times greater in the caudal segments. Lateral bending and axial rotation were coupled. Conclusions: Kinematics of the cranial and caudal cervical spine differed markedly with greater mobility in the caudal cervical spine. 相似文献
Upon intravenous transplantation, hematopoietic stem cells (HSCs) can home to specialized niches, yet most HSCs fail to engraft unless recipients are subjected to toxic preconditioning. We provide evidence that, aside from immune barriers, donor HSC engraftment is restricted by occupancy of appropriate niches by host HSCs. Administration of ACK2, an antibody that blocks c-kit function, led to the transient removal of >98% of endogenous HSCs in immunodeficient mice. Subsequent transplantation of these mice with donor HSCs led to chimerism levels of up to 90%. Extrapolation of these methods to humans may enable mild but effective conditioning regimens for transplantation. 相似文献