Expression of the Antimicrobial Peptide Piscidin 1 and Neuropeptides in Fish Gill and Skin: A Potential Participation in Neuro-Immune Interaction     

Giacomo Zaccone  Gioele Capillo  Jorge Manuel Oliveira Fernandes  Viswanath Kiron  Eugenia Rita Lauriano  Alessio Alesci  Patrizia Lo Cascio  Maria Cristina Guerrera  Michal Kuciel  Krystyna Zuwala  Jose Manuel Icardo  Atsushi Ishimatsu  Ryosuke Murata  Takafumi Amagai  Antonino German  Marialuisa Aragona 《Marine drugs》2022,20(2)

Antimicrobial peptides (AMPs) are found widespread in nature and possess antimicrobial and immunomodulatory activities. Due to their multifunctional properties, these peptides are a focus of growing body of interest and have been characterized in several fish species. Due to their similarities in amino-acid composition and amphipathic design, it has been suggested that neuropeptides may be directly involved in the innate immune response against pathogen intruders. In this review, we report the molecular characterization of the fish-specific AMP piscidin1, the production of an antibody raised against this peptide and the immunohistochemical identification of this peptide and enkephalins in the neuroepithelial cells (NECs) in the gill of several teleost fish species living in different habitats. In spite of the abundant literature on Piscidin1, the biological role of this peptide in fish visceral organs remains poorly explored, as well as the role of the neuropeptides in neuroimmune interaction in fish. The NECs, by their role as sensors of hypoxia changes in the external environments, in combination with their endocrine nature and secretion of immunomodulatory substances would influence various types of immune cells that contain piscidin, such as mast cells and eosinophils, both showing interaction with the nervous system. The discovery of piscidins in the gill and skin, their diversity and their role in the regulation of immune response will lead to better selection of these immunomodulatory molecules as drug targets to retain antimicrobial barrier function and for aquaculture therapy in the future.  相似文献   

Assessing the spawning season in common dentex (Dentex dentex) using microsatellites     

Yaisel J Borrell  Gloria Blanco  Emilia Vázquez  Jorge A Piñera  Gemma Gimenez  Alicia Estévez  & Jose A Sánchez 《Aquaculture Research》2008,39(12):1258-1267

A set of five variable microsatellite markers was used for the genetic characterization of two common dentex ( Dentex dentex ) broodstocks from an experimental hatchery and for the screening of the egg batches spawned during the complete spawn season in 2006. After parentage assignment, simulations were performed for the hatchery 1 broodstocks; the microsatellite set correctly assigned over 93% of descendants to parents when one parent was known and 80% when neither parent was known. Of the 261 eggs that the DNA was correctly amplified from, 254 were successfully assigned to a parental couple. More than one female or male took part (at the same point in time) in the spawning season. However, we observed 'dominant' individuals that produced high proportions of the descendants (unequal reproductive success) in all the sampling periods, although the contributions of these dominant individuals could change over time. This phenomenon reduces the effective breeding numbers, and could lead to inbreeding if this factor is not taken into account when the next parental generation is obtained. This factor should also be considered in aquaculture selection programmes because the dominant breeders may not be the most interesting individuals for commercial or productive schemes.  相似文献   

Census in April 1972 of the Atitlán Grebe (Podilymbus gigas), Guatemala     

Anne LaBastille  Jose Ovidio  Edgar Bauer 《Biological conservation》1973,5(1):62-63

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What controls fish functional diversity patterns in Colombian Andean streams?     

Jose Luis Poveda-Cuellar  Edwin Orlando Lpez Delgado  Pamela Tatiana Zúiga-Upegui  Francisco Antonio Villa Navarro 《Ecology of Freshwater Fish》2022,31(1):87-101

Understanding the mechanisms that structure communities have been a major challenge in ecological theory. Functional trait-based approaches are increasingly used for studying the processes underlying community assembly. However, species-poor systems of Andean streams these processes are largely unknown. Tropical Andean streams are highly variable in space-time and characterised by the harsh or stable environment, resulting in a high taxonomic and ecological diversity. In this study, we tested the influence of environmental variables on the fish functional structure. We proposed that in ecosystems with harsh environments, environmental filtering will structure fish assemblages, and in ecosystems with a more stable environment, limiting similarity will prevail, increasing functional diversity. We analysed fish assemblages using functional diversity indices, fourth-corner RLQ analyses and multiple regression models. Environmental variables such as altitude, stream width, sub-Andean vegetation and pH influenced functional diversity. For instance, high values of functional redundancy were registered at high elevation streams, reflecting fish assemblages composed of highly dominant species with similar functional traits such as species of the genus Astroblepus. However, we found differences between Astroblepus species in the number and distance of gill rakers which might indicate resource partitioning and coexistence. On the other hand, our results show both stochastic dynamics and environmental filtering are structuring fish assemblages in low and high elevation Andean streams respectively.  相似文献