莠去津原药中莠去津及主要杂质的气质联用分析研究     

黄修柱  季颖 《农药科学与管理》2003,24(3):8-10

本文通过采用气质联用(GC—MS)法对莠去津原药中莠去津及其它杂质进行分析鉴定，从而了解莠去津原药中的杂质情况和质量状况，用以指导工业生产。  相似文献   

Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis>     

Tsutomu MATSUMOTO  Yuichiro NARA  Hiromitsu FURUYA  Harumi TAKAHASHI  Kiichi TAIRAKO  Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384

L₁₁A-Fukushima (L₁₁A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L₁₁A-F and L₁₁A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L₁₁A-F appears to possess the properties necessary for practical use. To manage L₁₁A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L₁₁A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献   

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields     

Shohei MATSUURA  Shigeru HOSHINO  Hideaki HAYASHI  Tetsuyuki KOHGUCHI  Kyoji HAGIWARA  Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001  相似文献   

黄瓜无毛突变体的生理特性研究   总被引：4，自引：1，他引：4  

马德华  庞金安  温晓刚  李淑菊  霍振荣  林世青 《园艺学报》2002,29(3):282-287

 黄瓜无毛突变体与普通黄瓜相比, 幼苗的各种性状均没有明显变化; 成株矮化明显, 雌花数降低, 产量明显下降, 但雌花节率没有明显变化, 蒸腾速率、气孔导度较高, 其他光合作用指标没有明显变化。遗传研究表明, 无毛性状是由一对核基因控制的隐性性状。  相似文献   

Distribution of citrus viroids and <Emphasis Type="Italic">Apple stem grooving virus</Emphasis> on citrus trees in Japan using multiplex reverse transcription polymerase chain reaction     

Takao Ito  Nobuyuki Namba  Tsutae Ito 《Journal of General Plant Pathology》2003,69(3):205-207

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Indole-3-acetic acid biosynthesis in <Emphasis Type="Italic">Aciculosporium take</Emphasis>, a causal agent of witches' broom of bamboo     

Eiji Tanaka  Chihiro Tanaka  Atsushi Ishihara  Yasumasa Kuwahara  Mitsuya Tsuda 《Journal of General Plant Pathology》2003,69(1):1-6

 Aciculosporium take (Ascomycota; Clavicipitaceae) is a causal agent of witches' broom of bamboo plants. The symptoms of this disease are believed to be induced by plant hormones, particularly auxins. Indole-3-acetic acid (IAA) was identified in cultures of this fungus in an l-tryptophan-supplemented liquid medium. IAA production was confirmed on 30 isolates of A. take from various hosts and locations at levels up to 1 mg/l. The biosynthetic pathway of IAA in A. take culture was examined by analyzing intermediate products and by feeding experiments. The results showed that the indole-3-pyruvic acid pathway (l-tryptophan → indole-3-pyruvic acid → indole acetaldehyde → IAA) was the dominant pathway in A. take. Received: June 3, 2002 / Accepted: July 25, 2002  相似文献   

Inconspicuous restraint of rice seedling growth by root-infecting fungi in soil of a rice paddy field     

Hiromitsu Furuya  Tsutomu Matsumoto  Shin-ichi Fuji  Hideki Naito 《Journal of General Plant Pathology》2003,69(2):115-119

 Rice seedling growth, estimated by plant height and root development and discoloration, was better in pasteurized soil than in unpasteurized soil obtained from a flooded rice field. Rice seedlings also grew better in sterilized soil modified by adding roots harvested from the pasteurized soil than in soil modified by adding roots harvested from the unpasteurized soil. The results demonstrate that seedling growth in the rice field soil was inhibited by soil microorganisms, even though no typical symptoms such as seedling blight or damping-off appeared. Pythium aristosporum is suggested to be involved in the inhibition. Thus, it appears that inconspicuous restraint of rice seedling growth could occur in soils of rice paddy fields. Received: May 20, 2002 / Accepted: October 16, 2002 Acknowledgments The authors thank Dr. T. Ichitani, former professor at Osaka Prefectural University, for providing an isolate of Pythium aristosporum for comparison, and Mr. Mitsuaki Sato of Akita Prefectural College of Agriculture for technical assistance.  相似文献   

灵芝胞外多糖高产菌株筛选及其深层发酵培养基的优化   总被引：12，自引：2，他引：12  

宋频然  常继东 《食用菌学报》2003,10(2):9-16

运用反馈抑制理论构建了耐灵芝胞外多糖 (EPS)反馈抑制的筛选模型。添加在筛选培养基中的其指示因子同源胞外多糖浓度为 2 .34g/L。将实验室保藏的 37株灵芝菌株输入该模型 ,即可检出耐灵芝胞外多糖反馈抑制作用最强、产胞外多糖能力最强的菌株GL0 2 9。然后在基础培养基的基础上用正交试验方法优化GL0 2 9深层发酵培养基。结果表明 ,组合碳源和组合氮源培养基最适合该菌株深层发酵生产灵芝胞外多糖。深层发酵培养基配方为 :蔗糖 10 g/L ,玉米粉15 g/L ,蛋白胨 2 g/L ,酵母膏 1g/L ,KCl 0 .5g/L ,KH2 PO4 ·7H2 O 0 .5 g/L ,pH自然。于 30℃、12 0r/min摇瓶培养 9d ,该菌株的胞外多糖产量高达 3.0 7g/L。  相似文献   

江汉平原水稻白叶枯病菌致病型的变异研究   总被引：2，自引：0，他引：2  

钱汉良  陈其志 《植物病理学报》1997,27(4):333-337

 从水稻和稻田周围常见的10种杂草上来集梨孢属菌株,以涂沫法作交互接种,分离自牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐的梨孢菌与分离自水稻的梨孢菌可以交叉侵染其寄主,茭白和碎米莎草梨孢菌与稻梨孢菌不能交互侵染;自然诱发条件下来自牛筋草和狗尾草的梨孢菌与来自水稻的梨孢菌对其寄主同样发生交叉侵染。直接从杂草寄主上分离的梨孢菌株对水稻的致病性较弱,接种到水稻感病品种发病后再分离的菌株,对水稻致病性增强,对原寄主的致病性基本保持不变。推断稻瘟病区稻田周围牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐等杂草寄主上的梨孢菌在稻瘟病菌的积累和传播方面具有作用。  相似文献   

畜禽粪便恶臭的污染及其治理对策的探讨   总被引：3，自引：0，他引：3  

黄灿  李季 《家畜生态》2004,25(4):211-213,217

随着畜牧业生产规模的扩大和集约化程度的提高，畜禽粪便产生的恶臭所造成的危害已经引起人们的普遍关注。本文主要综述了畜禽粪便恶臭物质的组成、产生的影响因素及畜禽粪便恶臭的控制方法，并对畜禽粪便除臭存在的问题及发展趋势提出了一些看法。  相似文献