4株酸奶噬菌体的分离     

徐成勇  吴昊  顾晓琳  何楚莹  陶仲炎  秦玉青  周凌华  应杰  诸葛健  郭本恒 《乳业科学与技术》2004,26(4):147-149

从上海光明乳业股份有限公司车间发酵罐中分离出4株酸奶噬菌体。1号噬菌体头部呈六角形，平均直径为95.1nm，头部剖面平均面积为8184．8nm^2，尾部的平均直径为18．3nm，平均长度为403．4nm；2号噬菌体头部呈六角形，平均直径为85．4nm，头部剖面平均面积为8046．2nm^2，尾部的平均直径为23．3nm，平均长度为549．4nm；3号噬菌体头部呈六角形，平均直径为95．5nm，头部剖面平均面积为9792．3nm^2，尾部的平均直径为19．8nm，平均长度为450．5nm；4号噬菌体头部呈六角形，平均直径为119．3nm．头部剖面平均面积为12586．1nm^2，尾部的平均直径为23．6nm，平均长度为520．5nm。  相似文献   

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy     

K Chatdarong  N Kampa  E Axnr  C Linde‐Forsberg 《Reproduction in domestic animals》2002,37(5):275-281

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.  相似文献   

浅谈鸡球虫病的免疫   总被引：1，自引：0，他引：1  

齐怀忠  徐善文 《中国动物检疫》2002,19(7):21-22

鸡球虫病是一种危害性较大的肠道寄生虫病,多发于3个月龄以内的仔鸡.尤以15～45日龄的雏鸡最易感染,近年来,球虫疫苗应用,使工厂生产的养鸡场在鸡球虫病控制方面取得显著成绩.笔者就鸡球虫病免疫在生产实践中的应用,作肤浅论述.  相似文献   

用分子生物学方法鉴别检测动物源性饲料中的牛羊源性成分   总被引：10，自引：0，他引：10  

杨宝华  宗卉  林庆燕  俞火 《中国畜牧杂志》2002,38(1):3-5

为了防止海绵状脑病疫区和痒病疫区反刍动物源性饲料进入我国 ,非常有必要对贸易往来中的肉骨粉品种来源进行鉴定检测。我们采用分子生物学方法从动物源性饲料中鉴别检测牛、羊特异性线粒体DNA的片段 ,并用限制性内切酶SspⅠ对PCR产物进行酶切鉴定及DNA序列测定。该方法的灵敏度可达 0 .12 5 % ,具有灵敏度高、快速和特异性强的优点。  相似文献   

盐胁迫对4种禾本科牧草种子萌发和幼苗生长的影响     

覃广泉  陈平  苏东海 《勤云标准版测试》2005,(3)

通过NaCl溶液(0、1.5、3.0、6.0、9.0g/L)处理4种禾本科牧草种子,测定了种子萌发和幼苗生长有关指标。结果表明:美洲狼尾草较耐盐,在9.0g/L盐溶液处理下比其它品种维持了高的发芽率、发芽指数、活力指数,并且其相对值高,以及较高的相对苗长和根长,盐处理显著提高几种牧草幼苗质膜相对透性,总体上降低胚乳的利用速度和利用效率。  相似文献   

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis     

S. J. Casselman  A. I. Schulte‐Hostedde 《Ecology of Freshwater Fish》2004,13(3):217-222

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.  相似文献   

马立克氏病病毒囊膜糖蛋白B抗原Ⅰ型特异性和群共同性决定簇的单克隆抗体   总被引：3，自引：1，他引：2  

陆长明  崔治中  秦爱建  ＬｅｅＬＦ 《中国兽医学报》1997,(4)

用能表达马立克氏病病毒（ＭＤＶ）糖蛋白Ｂ（ｇＢ）的重组杆状病毒感染的Ｓｆ９细胞免疫小鼠，制备针对ＭＤＶｇＢ的单克隆抗体。以Ⅰ型马立克氏病病毒ＧＡ株感染的鸡胚成纤维细胞作为检测抗原，同时以免疫荧光试验（ＩＦＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）来筛选杂交瘤细胞，结果获得了ＩＦＡ和ＥＬＩＳＡ均为阳性的２株单克隆抗体细胞株，定名为ＢＡ４和ＢＤ８。在ＩＦＡ和免疫沉淀试验中，单抗ＢＤ８与Ⅰ、Ⅱ、Ⅲ型ＭＤＶ均呈阳性反应；单抗ＢＡ４只对Ⅰ型ＭＤＶ（包括ＣＶＩ９８８疫苗株）呈阳性反应。免疫沉淀反应进一步确证２株单抗识别的是ＭＤＶ糖蛋白Ｂ抗原。  相似文献   

暴发高致病禽流感疫区鸟类禽流感的血清学调查   总被引：1，自引：0，他引：1  

李曦  符芳  王琴  童光志 《中国兽医杂志》2006,42(12):3-5

本研究应用血凝抑制试验(H I),2004年初H 5亚型禽流感在中国内地发生前后,于湖北、广西二疫区市县的生态系中,自健康的鹰、鸽子、野鸭和鹌鹑中采集了54份血清,对其进行了血清学检测,并将获得的血清学检测数据进行了统计学分析。分析结果显示,在总共采集的54份血清样品中,抗H 6亚型禽流感病毒(H 6)抗体、抗H 9亚型禽流感病毒(H 9)抗体、抗H 5亚型禽流感病毒(H 5)抗体阳性率分别为64.8%、75.9%、29.6%。推断,这一时期,H 6亚型禽流感病毒(H 6)、H 9亚型禽流感病毒(H 9)和H 5亚型禽流感病毒(H 5)共循环于该生态系中,其中H 9、H 6亚型禽流感病毒长期、稳定循环于该生态系中,为该生态系禽流感主要流行血清型。血凝抑制试验检测结果发现,H 5亚型禽流感病毒抗体滴度较低,最高仅为640×,其阳性率为29.6%,推断其可能为新近传入的病毒或新近重组变异出现的新病毒,可能为与该次禽流感暴发相关的新病毒,这一推断还有待于通过对病毒基因序列的分析而做进一步验证。  相似文献   

奶牛胎儿的父亲对母亲产奶量的影响     

秦志锐  杨建尧  张沅  江邦岚  张守仁 《畜牧兽医学报》1992,(4)

用中国黑白花奶牛研究了胎儿的父亲及犊牛初生重对母亲当胎及下一个泌乳期产奶量的影响,结果指出胎儿的父亲对母亲当胎及下一个泌乳期90天和305天的产奶量,都有极显著的影响,各占产奶总变异量的9.4％,15.3％及3.77％(P<0.01)。犊牛初生重对母亲产奶量的影响也显著(P<0.05)。当根据公牛的产奶预期差(PDM)将胎儿的父亲分为两组后,发现差异显著(P<0.05),胎儿父亲的PDM高,胎儿母亲的产奶量也高,因此胎儿母亲产奶量不仅受胎儿重量的影响,也受父亲PDM的影响。  相似文献   

快速高效蚕桑之路初探     

秦凌波 《蚕学通讯》2001,21(2):20-21

1　起因残疾人秦廷焕家住丰都县十直镇张家坝村。该村离镇场较远 ,属贫困村之一。常年种植水稻、玉米、小麦、红苕、油菜、榨菜、少部分水果 ,养殖生猪、耕牛、山羊等。经济骨干品种较少 ,人均粮食不足 50 0公斤 ,经济收入不足 1 ,0 0 0元。“要想富、先修路” ,多年贫困 ,吃尽路不通苦头的秦廷焕于去冬今春拿出自己的积蓄和借款计 4万余元修通了该村3社至砒邻的树人镇熊山村 5社四公里的断头路 ,实施了他人生路当中的第一步计划。修路被评为劳模的他今年“五·一”劳动节在重庆市劳模会上听取了贺书记、包市长关于西部开发、农业结构调整 ,…  相似文献