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排序方式: 共有333条查询结果,搜索用时 15 毫秒
81.
Olivry T Steffan J Fisch RD Prélaud P Guaguère E Fontaine J Carlotti DN;European Veterinary Dermatology Cyclosporine Group 《Journal of the American Veterinary Medical Association》2002,221(3):370-377
OBJECTIVE: To evaluate efficacy of cyclosporine A, administered at either of 2 dosages, in dogs with atopic dermatitis (AD). DESIGN: Multicenter randomized controlled trial. ANIMALS: 91 dogs with AD. PROCEDURE: Dogs were assigned to receive placebo (30 dogs), cyclosporine at a low dosage (2.5 mg/kg [1.1 mg/lb], PO, q 24 h for 6 weeks; 30 dogs), or cyclosporine at a high dosage (5.0 mg/kg [2.3 mg/lb], PO, q 24 h for 6 weeks; 31 dogs). RESULTS: After 6 weeks, mean percentage reductions, compared with baseline scores, in scores of lesion severity were 34, 41, and 67% for dogs treated with the placebo, cyclosporine at the low dosage, and cyclosporine at the high dosage, respectively. Similarly, mean percentage reductions in pruritus scores were 15, 31, and 45%, respectively. Percentage reductions in skin lesion and pruritus scores were significantly higher for dogs given cyclosporine at the high dosage than for dogs given the placebo. Treatment efficacy was significantly associated with whether dogs had a history of seasonal AD. Percentage reductions in skin lesion and pruritus scores were high for dogs treated with cyclosporine at the highest dosage that had a history of nonseasonal AD. Dogs in all groups with seasonal AD improved during the study period. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that oral administration of cyclosporine at a dosage of 5.0 mg/kg once daily is effective in reducing severity of pruritus and skin lesions in dogs with AD, especially those with nonseasonal disease. 相似文献
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Regnier A Schneider M Concordet D Toutain PL 《American journal of veterinary research》2008,69(3):410-415
OBJECTIVE: To compare penetration of IV administered marbofloxacin in intraocular fluids of healthy and inflamed eyes in rabbits with endotoxin-induced endophthalmitis. ANIMALS: 35 pigmented rabbits. PROCEDURES: Endophthalmitis was induced in the right eye via intravitreal administration of Escherichia coli endotoxin. The left eye was a control eye. After 24 hours, a single dose of marbofloxacin (4 mg/kg, IV) was administered. Groups of rabbits (n = 5/group) were euthanized 0.5, 1, 2, 4, 6, 10, and 18 hours later, and blood and ocular fluids were collected. Marbofloxacin concentrations were determined via reverse-phase high-performance liquid chromatography, and pharmacokinetic analysis of the data was performed with a mono-compartmental model. RESULTS: Mean area under the aqueous concentration-time curve was significantly lower in control eyes (1.64 +/- 0.07 microg*h/mL) than in inflamed eyes (3.31 +/- 0.11 microg*h/mL). Similarly, drug penetration into aqueous humor was 33% and 65% for control eyes and inflamed eyes, respectively. Mean area under the vitreous humor concentration-time curve for control eyes(1.75 +/- 0.05 microg*h/mL) was significantly less than for inflamed eyes (2.39 +/- 0.16 microg*h/mL). In the vitreous humor, corresponding penetrations were 34% and 47%, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Penetration of marbofloxacin into the aqueous and vitreous humor after IV administration was significantly enhanced by intraocular inflammation, suggesting a role for this antimicrobial in the prophylaxis or treatment of bacterial endophthalmitis caused by susceptible pathogens. 相似文献
84.
A methodology for assessing the risk posed by the deliberate and harmful use of plant pathogens in Europe 总被引:1,自引:0,他引:1
Emilie Latxague Ivan Sache Jean Pinon Didier Andrivon Marc Barbier Frédéric Suffert 《EPPO Bulletin》2007,37(2):427-435
Deliberate and malevolent use of plant pathogens, i.e. agroterrorism lato sensu (anticrop bioterrorism and use of bioweapons against the agricultural sector), may represent a non-negligible threat for crops and forests in Europe. In order to assess this risk objectively, a methodology based on a critical review of existing Pest Risk Analysis schemes has been elaborated and is described in the present paper. In this methodology, three interdependent steps are suggested: (i) definition of an a priori list of potentially dangerous plant pathogens for Europe, (ii) detailed elaboration and analysis of theoretical scenarios of possible acts of agroterrorism, and (iii) elaboration and use of a risk assessment scheme adapted to agroterrorism. 相似文献
85.
Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction
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Tatiana Art Thierry Franck Pierre Lekeux Brieuc de Moffarts Laurent Couëtil Martine Becker Serge Kohnen Ginette Deby-Dupont Didier Serteyn 《Canadian journal of veterinary research》2006,70(4):291-296
The aim of this work was to measure the myeloperoxidase (MPO) concentration in bronchoalveolar lavage (BAL) fluid collected from horses with recurrent airway obstruction (RAO), both in crisis and in remission, as well as from healthy horses. Seven horses with RAO were exposed to moldy hay until the maximum change in pleural pressure was greater than 1.5 kPa. At that point, BAL was performed, and the total cell counts and percentages in the fluid were immediately determined. To measure the MPO concentration in BAL-fluid supernatant, we used a specific enzyme-linked immunosorbent assay with polyclonal antibodies against equine MPO. The tests were repeated on the horses with RAO after they had spent 2 mo on pasture. Six healthy horses serving as controls underwent the same tests. The absolute and relative neutrophil counts and the MPO concentration in the BAL fluid were significantly greater in the horses with an RAO crisis than in the control horses. After 2 mo on pasture, the horses that had been in RAO crisis were clinically normal, and their neutrophil counts and MPO levels in BAL fluid had significantly decreased; during remission their neutrophil counts were not significantly different from those in the healthy horses, but their MPO concentration remained significantly higher. This study showed that determining the MPO concentration in a horse's BAL fluid is technically possible and that during remission from RAO the concentration remains higher than normal. Thus, MPO may be a marker of neutrophil presence and activation in the lower airways. 相似文献
86.
Dupont D Rolet-Repecaud O Muller-Renaud S 《Journal of agricultural and food chemistry》2004,52(4):677-681
Dairy industries are interested in knowing the heat treatment undergone by milk so as to control the quality of drinking milks or to control their heating systems. Among the different techniques available to characterize the heat treatment of milk, estimation of the denaturation of proteins has been widely used. However, because the concentration of the proteins in raw milk can fluctuate significantly, determining only the concentration of a native protein without knowing its concentration in the raw milk before undergoing heat treatment can lead to significant imprecision. The objective of this study was to develop, on Biacore 3000, a biosensor assay for determining the denaturation index of alpha-lactalbumin by quantifying separately the native and "heat-denatured" forms of alpha-lactalbumin with specific monoclonal antibodies. alpha-Lactalbumin denaturation index is independent of the concentration of alpha-lactalbumin in the original raw milk. The technique developed is discriminating, fast, repeatable, fully automated, and requires no pretreatment of the milk sample. 相似文献
87.
Resistance Evaluation of Some Chinese Leading Rice Maintainer, Restorer lines and Their Hybrids to Magnaporthe grisea 总被引:1,自引:0,他引:1
ZHUXu-dong SHENYing HenriADREIT JulienFROUIN DidierHARREAU 《水稻科学》2004,11(3):101-105
Six isolates of Magnaporthe grisea were selected to inoculate on 10 Chinese leading maintainer lines (B-lines), 14 restorer lines (R-lines) and their F1 hybrid plants. In the tested rice materials, R-lines were proved to be more resistant to blast than B-lines. The resistance frequency of about 25% F1 hybrid plants was less than their parents. In addition, 26 isolates of M. grisea collected from different rice growing areas of China were inoculated on 13 new improved hybrid rice combinations. The resistance frequencies of 5 improved hybrids were better than those of the controls and leading varieties in rice production of China. 相似文献
88.
Kanika Singh Hitesh B. Vasava Didier Snoeck Bhabani S. Das David Yinil Damien Field Todd Sanderson Chris Fidelis Israr Majeed Niranjan Panigrahi 《Soil Use and Management》2019,35(3):492-502
In this study, diffuse reflectance spectroscopy (DRS) approach was examined for making input recommendations in the smallholder cocoa farms of Papua New Guinea (PNG). Soil samples were collected from four provinces of PNG. Soil samples from four different depths (0–10, 10–30, 30–60 and 60–90 cm) of 32 profiles in each of these site were used to create a database of soil chemical and physical properties. Spectral reflectance values at 1 nm interval covering visible to shortwave‐infrared (350–2,500 nm) were collected for each of these soil samples to develop partial least squares regression models. Soil textural fractions, soil organic carbon contents and available N were well predicted by the DRS approach with R2 values larger than 0.75. Moderate to poor estimation efficiencies were observed for remaining parameters. Nevertheless, the estimated soil attributes and their corresponding measured soil parameters were used as inputs to an input recommendation model of soil diagnosis to create input recommendation for a targeted cocoa yield of 1,000 kg dry cocoa beans ha‐1 Resulting input recommendations were similar for both of these input sources (measured and DRS‐estimated) suggesting that the DRS approach may provide an easy way to create input recommendations. 相似文献
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90.