Summary Seven wild
Petunia species including 2
n = 18 species (
P. parviflora Jussieu,
P. linearis Hook.) and those with 2
n = 14 (
P. parodii Steene,
P. axillaris Lam.,
P. integrifolia Hook.,
P. inflata R.E. Fries,
P. violacea Lindl.) and ten
Petunia hybrida horticultural lines were compared for polymorphisms in rDNA genes using the four restriction enzymes
EcoRI,
BamHI,
HindIII and
XhoI. All the unit types found in the lines pre-existed in the wild forms. There are two different sizes of either 11.45 or 11.6
kb./The 2
n = 18 species are closely related to the 2
n = 14 species, thus making the
Petunia genus homogeneous. Moreover, it is likely that
P. hybrida lines originated in several kinds of crosses between these species. We constructed a dendrogram for all the 15 rDNA unit
types found. Two main branches of the tree result from the presence or the absence of
HindIII sites. The main branch is divided according to variability at the
EcoRI and
BamHI sites. Taking into account the existence of several loci which carry one unit type only, we consider whether or not exchanges
might occur between loci. Lines carrying two unit types and lines carrying three unit types support such a hypothesis.
XhoI and
BamHI fragments enable us to distinguish two types of 5S DNA corresponding to 2
n = 18 and 2
n = 14 species, respectively.
P. hybrida lines and each 2
n = 14 wild species carry one of the types only, that corresponds to one 5S DNA locus. The most parsimonious phylogenetic trees
whatever the species chosen as the outgroup, do not fit with our knowledge of
Petunia and with taxonomy. This is likely because only few loci formed the basis of these phylogenetic constructions.
相似文献