Trypanocidal properties of Mikania stipulacea and Mikania hoehnei isolated terpenoids     

do Nascimento AM  Siqueira Chaves J  Albuquerque S  Rodrigues de Oliveira DC 《Fitoterapia》2004,75(3-4):381-384

The in vitro trypanocidal properties of isolated terpenoids from Mikania stipulacea and Mikania hoehnei were investigated in Trypanosoma cruzi Y strain. Both compounds, the diterpene ent-9alpha-hydroxy-15beta-E-cinnamoyloxy-16-kauren-19-oic acid (1) and the sesquiterpene lactone 8beta-hydroxyzaluzanin D (2) were active.  相似文献   

Mineral composition and microstructure of the abaxial hoof wall in dairy heifers after biotin supplementation     

Paulo José Bastos Queiroz  Bruno Moraes Assis  Danilo Conrado Silva  Antônio Dionísio Feitosa Noronha Filho  Alexandre Pancotti  Rogério Elias Rabelo  Naida Cristina Borges  Valcinir Aloisio Scalla Vulcani  Luiz Antônio Franco da Silva 《Anatomia, histologia, embryologia》2021,50(1):93-101

The aim of this study was to evaluate the effect of daily biotin supplementation on the mineral composition and microstructure of the abaxial hoof wall in dairy heifers. The heifers were housed on a concrete floor and fed for weight gain more than 800 g per day, which is a challenging environment for the hoof. Twelve crossbred dairy heifers (Jersey × Holstein) were divided into two treatment groups. Animals in the control group (n = 6) received a diet without supplemental biotin, while the heifers in the biotin-supplemented feed group (n = 6) each received 20 mg of biotin daily for 120 days. Samples of the abaxial hoof wall were collected from the outer claw of the fore and hind limb, before and after supplementation. The samples were evaluated by X-ray fluorescence spectrometry, computed microtomography, atomic force microscopy and confocal laser scanning microscopy. Biotin supplementation increased the sulphur content and decreased the calcium and potassium content in the abaxial hoof wall. Biotin treatment also increased the percentage of horn tubules with smaller diameter marrow (17–51 µm). However, biotin did not influence the surface relief of the hoof wall, suggesting that its action is limited to the inner layers of the stratum corneum. Daily supplementation with 20 mg of biotin promoted changes in the mineral composition and microstructure of abaxial hoof wall of crossbred dairy heifers. These findings suggest biotin supplementation improves hoof quality and may help to understand the function of biotin in the stratum corneum.  相似文献   

Canine otitis externa caused by the fungus Sporothrix schenkii.          下载免费PDF全文

W M Dion  G Speckmann 《The Canadian veterinary journal. La revue veterinaire canadienne》1978,19(2):44-45

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The origin and species of yeasts in commercial preparations of bovine semen.          下载免费PDF全文

W M Dion 《Canadian journal of veterinary research》1979,43(1):16-21

Whereas yeasts were not normally isolated from raw semen samples 13% of commercial frozen semen samples and 71% of preputial washings contained yeasts. Nine genera and 25 species of yeasts have been identified from these two sources. Yeasts originating in the preputial cavity were generally saprobic members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis and Trichosporon. Those originating as contaminants during processing were more likely to be opportunistic pathogens of the genus Candida. Conception was not necessarily affected by the presence of large numbers of Candida krusei or C. macedoniensis in the uterus.  相似文献   

Genetic variation of germination cold tolerance in Japanese rice germplasm     

Fátima Bosetti  Camila Montebelli  Ana Dionísia L.C. Novembre  Helena Pescarin Chamma  José Baldin Pinheiro 《Breeding Science》2012,62(3):209-215

Low temperatures at the initial stages of rice development prevent fast germination and seedling establishment and may cause significant productivity losses. In order to develop rice cultivars exhibiting cold tolerance, it is necessary to investigate genetic resources, providing basic knowledge to allow the introduction of genes involved in low temperature germination ability from accessions into elite cultivars. Japanese rice accessions were evaluated at the germination under two conditions: 13°C for 28 days (cold stress) and 28°C for seven days (optimal temperature). The traits studied were coleoptile and radicle length under optimal temperature, coleoptile and radicle length under cold and percentage of the reduction in coleptile and radicle length due to low temperature. Among the accessions studied, genetic variation for traits related to germination under low temperatures was observed and accessions exhibiting adequate performance for all investigated traits were identified. The use of multivariate analysis allowed the identification of the genotypes displaying cold tolerance by smaller reductions in coleoptile and radicle lenght in the presence of cold and high vigour, by higher coleoptile and radicle growth under cold.  相似文献   

Feline phaeohyphomycosis: treatment with ketaconazole and 5-fluorocytosine          下载免费PDF全文

Pukay BP  Dion WM 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(3):130-134

Two cats with phaeohyphomycosis, one infected with Phialophora verrucosa and the other with Exophiala jeanselmei, were treated with ketaconazole alone and in combination with 5-fluorocytosine after recurrence of the infections following surgical excision. The drugs were given orally at various doses and for various lengths of time, but were ineffective. Hepatocellular damage occurred in one cat.  相似文献   

Use of fungassay medium in the diagnosis of ringworm          下载免费PDF全文

Dion WM 《The Canadian veterinary journal. La revue veterinaire canadienne》1978,19(7):203-204

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Different DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls     

Nawapen Phutikanit  Junpen Suwimonteerabutr  Dion Harrison  Michael D'Occhio  Bernie Carroll  Mongkol Techakumphu 《Acta veterinaria Scandinavica》2010,52(1):18

Background

The purpose of this study was to apply an arbitrarily primed methylation sensitive polymerase chain reaction (PCR) assay called Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) to investigate the methylation profiles of somatic and germ cells obtained from Holstein bulls.

Methods

Genomic DNA was extracted from sperm, leukocytes and fibroblasts obtained from three bulls and digested with a methylation sensitive endonuclease (HpaII). The native genomic and enzyme treated DNA samples were used as templates in an arbitrarily primed-PCR assay with 30 sets of single short oligonucleotide primer. The PCR products were separated on silver stained denaturing polyacrylamide gels. Three types of PCR markers; digestion resistant-, digestion sensitive-, and digestion dependent markers, were analyzed based on the presence/absence polymorphism of the markers between the two templates.

Results

Approximately 1,000 PCR markers per sample were produced from 27 sets of primer and most of them (>90%) were digestion resistant markers. The highest percentage of digestion resistant markers was found in leukocytic DNA (94.8%) and the lowest in fibroblastic DNA (92.3%, P ≤ 0.05). Spermatozoa contained a higher number of digestion sensitive markers when compared with the others (3.6% vs. 2.2% and 2.6% in leukocytes and fibroblasts respectively, P ≤ 0.05).

Conclusions

The powerfulness of the AMP PCR assay was the generation of methylation-associated markers without any prior knowledge of the genomic sequence. The data obtained from different primers provided an overview of genome wide DNA methylation content in different cell types. By using this technique, we found that DNA methylation profile is tissue-specific. Male germ cells were hypomethylated at the HpaII locations when compared with somatic cells, while the chromatin of the well-characterized somatic cells was heavily methylated when compared with that of the versatile somatic cells.  相似文献   

Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts     

Dina Neves  Paula Caetano  Joana Oliveira  Cristiana Maia  Marília Horta  Nelson Sousa  Marco Salgado  Lídia Dionísio  Naresh Magan  Alfredo Cravador 《European journal of plant pathology / European Foundation for Plant Pathology》2014,138(4):835-846

Phlomis purpurea (Lamiaceae), found in Quercus suber and Quercus ilex ssp. rotundifolia forest habitats in southern Portugal, is a non-host for the oomycete Phytophthora cinnamomi, the main biotic factor involved in cork oak and holm oak decline in the Iberian Peninsula. The effect of P. purpurea crude ethanol root extract was evaluated in vitro on P. cinnamomi mycelial growth, sporangial production, zoospore release and germination as well as on chlamydospore production and viability. The protection of cork oak against infection by the pathogen was also evaluated in planta. At 10 mg ml^?1, in vitro inhibition of the pathogen structures was 85–100 %. In addition, P. purpurea plants were shown to protect Q. suber and Q. ilex from P. cinnamomi infection and to reduce the inoculum potential in glasshouse trials, indicating the ability to reduce root infection by the pathogen. The results suggest that P. purpurea has the potential to reduce disease spread and that their root extracts could provide candidate substances for control of the important pathogen, P. cinnamomi.  相似文献   

Seasonal dynamics of microbial community in an aquaculture system for Nile tilapia (Oreochromis niloticus)          下载免费PDF全文

Juliana Alves Resende  Vânia Lúcia Silva  Dionéia Evangelista Cesar  Alessandro Del'Duca  Cláudia Oliveira Fontes  Cláudio Galuppo Diniz 《Aquaculture Research》2015,46(5):1233-1240

Studies on bacterial abundance and diversity will improve the understanding of the microbial ecology to optimize aquaculture production, water quality, disease control and environmental impact of effluents. We comparatively evaluated an aquaculture system in dry and rainy seasons by Fluorescent in situ Hybridization (FISH) and culture‐dependent methodology. Overall, a negative correlation between seasonality in bacterial and flagellates abundance was observed. Alpha‐, beta‐, gamma‐proteobacteria and Cytophaga‐Flavobacter were observed with seasonal variation. Putative pathogenic bacteria such as coagulase‐negative Staphylococcus, non‐fermenter Gram‐negative rods (Pseudomonas sp. and Burkholderia sp.), and Enterobacteriaceae were also detected in this study with significant seasonal variation. Focusing on medically important bacteria, our data show that microbial diversity in the environment associated with aquaculture, as it is practiced, may be altered in growth ponds used for fish cultivation. As an ecological consequence, potentially pathogenic bacteria might be released in high concentrations to the downstream environments posing potential threats to human and animal health.  相似文献