Biochemical and morphological developments are partially impaired in intestinal mucosa from growing pigs fed reduced-protein diets supplemented with crystalline amino acids   总被引：2，自引：0，他引：2  

Guay F  Donovan SM  Trottier NL 《Journal of animal science》2006,84(7):1749-1760

The objective of this study was to determine if a reduction in dietary CP, with partial replacement of the intact protein with crystalline AA (CAA), would alter growth, morphology, and free or peptide-bound AA concentrations of intestinal mucosa in growing pigs. Twenty-four barrows (37.0 +/- 1.5 kg of BW) were fed 1 of 4 diets for 24 d: 16.1% CP with no CAA, or 12.8, 10.1, or 7.8% CP (analyzed values, as-fed) containing CAA. As CP decreased, CAA were gradually increased to meet requirements on a true ileal digestible basis. Pigs were euthanized 2 h postmeal on d 24, and mucosal samples from duodenum, jejunum, and ileum were collected. Reducing dietary CP decreased ADG, G:F, and final weight (linear, P < 0.05). With reduced dietary CP, mucosal protein concentration decreased in the jejunum (quadratic, P < 0.05) and tended to decrease in the ileum (linear, P = 0.062). Reduction of the dietary CP concentration from 16.1 to 7.8% tended to decrease the crypt depth (linear, P < 0.10) and decreased villus width (linear, P < 0.05) in duodenum and jejunum mucosa but did not reduce villus height or villus surface area in any regions of the small intestine. In the duodenum, a reduction in dietary CP increased free Lys, Met, and Thr (linear, P < 0.05) and peptide-bound Lys and Thr (quadratic, P < 0.10). In the jejunum, reducing CP decreased free Cys (linear P < 0.05) and tended to decrease free Asn and His (linear, P < 0.10) and peptide-bound His (quadratic, P = 0.061) and Ile, Leu, and Val (linear, P < 0.10). In the ileum, reducing CP decreased free Asn, Ser, Tyr, Arg, His, Phe (linear, P < 0.05), and Leu (linear, P = 0.054) and peptide-bound Gly and Ser (linear, P < 0.05) and tended to decrease peptide-bound Ile, Leu, Phe, Val (linear, P < 0.10), and Lys (linear P < 0.05). In conclusion, reduced-CP diets supplemented with CAA lead to a reduction in growth performance, associated with biochemical and morphological modifications of the intestinal mucosa.  相似文献   

Effect of administration of a controlled-release monensin capsule on incidence of calving-related disorders, fertility, and milk yield in dairy cows     

Melendez P  Goff JP  Risco CA  Archbald LF  Littell RC  Donovan GA 《American journal of veterinary research》2006,67(3):537-543

OBJECTIVE: To determine the effect of a controlled-release monensin capsule administered at cessation of lactation on incidence of calving-related disorders, fertility, and milk yield in dairy cows. ANIMALS: 290 dairy cows treated with monensin and 290 untreated control cows. PROCEDURE: Treated cows received a capsule that released monensin at 335 mg/d for 95 days. Incidence of calving-related disorders; daily milk yield up to 20 days postpartum; test-day milk yield, fat, protein, and mature-equivalent 305-day milk production; and body condition score at calving were determined. Reproductive variables were conception rate at first service, pregnancy rate, and calving-to-conception interval. RESULTS: Cows treated with monensin were 2.1 times as likely to develop dystocia and 0.8 times as likely to develop metritis as control cows. For milk yield, there was an interaction of treatment X time X parity. In multiparous cows, monensin significantly improved milk yield at test days 4 and 7. In addition, monensin increased body condition score at calving. CONCLUSIONS AND CLINICAL RELEVANCE: Despite increasing the likelihood of developing dystocia and metritis, administration of monensin improved the lactational performance of multiparous cows and may be a promising additive for use at the time of cessation of lactation.  相似文献   

Rapid reversed phase ultra-performance liquid chromatography analysis of the major cocoa polyphenols and inter-relationships of their concentrations in chocolate     

Cooper KA  Campos-Giménez E  Jiménez Alvarez D  Nagy K  Donovan JL  Williamson G 《Journal of agricultural and food chemistry》2007,55(8):2841-2847

Chocolate and other cocoa-containing products are a rich source of polyphenols. This paper describes an ultra-performance liquid chromatography (UPLC) method that can separate and quantify in 3 min six of the major chocolate polyphenols: catechin; epicatechin; B2 (epicatechin-4beta-8-epicatechin); B5 (epicatechin-4beta-6-epicatechin); C1 (epicatechin-4beta-8-epicatechin-4beta-8-epicatechin); and tetramer D (epicatechin-4beta-8-epicatechin-4beta-8-epicatechin-4beta-8-epicatechin). A survey of 68 chocolate samples indicated that there was a strongly predictive relationship between epicatechin and the other individual polyphenols, especially procyanidin B2 (R 2 = 0.989), even though the chocolates came from varied sources and manufacturers. The relationship was less strong with catechin, and so further work to explore the reasons for this difference was performed. Chiral analysis on a subset of 23 chocolates showed that (-)-epicatechin had a predictive relationship with (+)-catechin in line with the other polyphenols, but not with (-)-catechin (the predominant form). This indicates that (-)-catechin is the most affected by manufacturing conditions, possibly formed through epimerization from (-)-epicatechin during processing. The results show that epicatechin concentrations can be used to predict the content of other polyphenols, especially B2 and C1, and total polyphenols content. Finally, the (-)-catechin content is not predictable from the epicatechin content, and it is concluded that this is the main form of polyphenol that varies according to manufacturing conditions and cocoa origin.  相似文献   

The peri‐urban Pacific: From exclusive to inclusive cities     

Donovan Storey 《Asia Pacific viewpoint》2003,44(3):259-279

Abstract: As the Pacific Islands continue to urbanise, existing models of governance and planning are coming under greater pressure and scrutiny. Both the city council approach and the ‘good’ urban governance agenda of donors have weaknesses in the region, especially in dealing with peri‐urban settlements where the most rapid urban population growth is occurring. This is resulting in increased social discontent and conflict. This paper critiques the ways in which Pacific Island towns and cities are governed and calls for an approach which is more inclusive (and less hierarchical) and informed by concepts of citizenship and social justice. Indeed, policy makers will need to broaden their concepts and practices of governance if many Pacific cities are to be socially, politically, and envir‐onmentally sustainable. However, the political‐economy of urban development in the region is not proving conducive to consensus, with conflict a more likely outcome in the foreseeable future.  相似文献   

Galapagos: John Kricher (Ed.), Smithsonian Institution, Washington, DC, 2003, ISBN 1-58834-041-4, 256 pages, Price: £31     

Alan Tye 《Biological conservation》2004,120(4):602

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Phosphorus release during decomposition of crop residues under conventional and zero tillage     

N.Z. Lupwayi  G.W. Clayton  J.T. O&#x;Donovan  K.N. Harker  T.K. Turkington  Y.K. Soon 《Soil & Tillage Research》2007,95(1-2):231-239

Field experiments were conducted at Fort Vermilion (58°23′N 116°02′W), Alberta, to determine phosphorus (P) release patterns from red clover (Trifolium pratense) green manure (GM), field pea (Pisum sativum), canola (Brassica rapa) and monoculture wheat (Triticum aestivum) residues in the 7th and 8th years of conventional and zero tillage. Phosphorus contained in crop residues ranged from 1.5 kg ha⁻¹ in pea to 9.2 kg ha⁻¹ in clover GM, both under zero tillage. The patterns of P release over a 52-week period sometimes varied with tillage, i.e., a greater percentage of GM residue P was released under conventional tillage than under zero tillage in the first 2–10 weeks of residue placement. Wheat residues resulted in net P immobilization under zero tillage, but the amounts immobilized were less than 1 kg ha⁻¹. When net P mineralization occurred, the percentage of P released ranged from 24% of wheat P under conventional tillage to 74% of GM P under conventional tillage. The amounts of P released were 0.4 kg ha⁻¹ from wheat, 0.8 kg ha⁻¹ from canola, 0.4 kg ha⁻¹ from pea and 5.1–5.6 kg ha⁻¹ from clover GM residues. Therefore, only GM residues recycled agronomically significant amounts of P for use by subsequent crops in rotation. Phosphorus release was positively correlated with residue P concentration and negatively correlated with C/P and lignin/P ratios.  相似文献   

The assessment of point and diffuse metal pollution of soils from an urban geochemical survey of Sheffield, England   总被引：7，自引：0，他引：7  

B.G. Rawlins  R.M. Lark  K.E. O'Donnell  A.M. Tye  T.R. Lister 《Soil Use and Management》2005,21(4):353-362

Abstract. A model of soil variability as a continuous background process with superimposed point contamination was applied to 569 measurements of metal concentrations (Cr, Ni and Pb) in the topsoils of Sheffield, England. Robust estimators of the variogram were shown to be required to describe spatial variation of the metal concentrations at most sampled locations. This is diagnostic of the presence of a contaminant process. Values of the standardized kriging error from the cross‐validation of each datum were used to identify spatial outliers for each metal. The ordinary kriged estimates of Cr, Ni and Pb were mapped after removing the outliers to estimate the background variation. Each of the 35 spatial outliers that occured in gardens have concentrations exceeding their Soil Guideline Value for residential land use with plant uptake, highlighting a potentially significant exposure pathway. The frequent observation of coal and furnace waste at these sites suggests that their dispersal, following domestic use and industrial processes, respectively, represents a significant point contaminant process. There was no evidence for spatial clustering of the point process. However, the spatial outliers of Cr and Ni showed a significant association with disturbed sites identified from historical land use maps, in part due to their prevalence in areas of historical steel manufacture. The magnitude of diffuse pollution for each metal in the urban soil was estimated by removing the spatial outliers and comparing robust measures of location with those from a survey of soils developed over the same parent materials in adjacent rural and peri‐urban environments. The Winsorized mean Pb concentrations in urban topsoil (203 mg kg^?1) were twice the value in the rural environment (101 mg kg^?1), highlighting a very substantial diffuse Pb load to urban soils. The equivalent estimated diffuse components in urban soils for Cr and Ni were, respectively, 25% and 14% higher than the rural soils.  相似文献   

Tree invasion in naturally treeless environments: Impacts of quinine (Cinchona pubescens) trees on native vegetation in Galápagos     

Heinke Jger  Alan Tye  Ingo Kowarik 《Biological conservation》2007,140(3-4):297-307

Impacts of plant invasions are largely scale-dependent and responses to the same exotic species may vary among communities. Since impacts caused by individual trees could anticipate consequences of a closed canopy of an invader, we studied the response of Galápagos native plants to quinine (Cinchona pubescens) trees in two vegetation zones. Quinine has invaded >11,000 ha of Santa Cruz Island, including the Miconia- and Fern-Sedge-Zones. We analysed species composition and abundance along transects radiating from the trunks of individual quinine trees. Species richness and percentage cover decreased significantly with proximity to individual trees, and these effects were more pronounced in the Fern-Sedge Zone than in the Miconia Zone. Cover of endemic and native herb species and grass species significantly declined by 57–88% in the Fern-Sedge Zone. This was not the case in the Miconia Zone, but here the dominant endemic Miconia robinsoniana decreased by 41%. Quinine is a major driver of plant community change in both vegetation zones. The greater susceptibility of species in the Fern-Sedge Zone was ascribed to the presence of a new growth form: quinine trees in a formerly treeless environment. Species of the Miconia Zone appeared to be better pre-adapted to higher shade levels created by the Miconia shrubs. Our results emphasize the need for future control of quinine to preserve the diversity of the native Galápagos flora.  相似文献   

Retrospective study of proliferative urethritis in dogs: Clinical presentation and outcome using various treatment modalities in 11 dogs     

Max Emanuel  Allyson C. Berent  Chick Weisse  Taryn Donovan  Kenneth E. Lamb 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):312-320

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Integrating community gardens into public parks: An innovative approach for providing ecosystem services in urban areas     

Isaac Middle  Peta Dzidic  Amma Buckley  Dawn Bennett  Marian Tye  Roy Jones 《Urban Forestry & Urban Greening》2014,13(4):638-645

When planned coherently, urban green spaces have the potential to provide cities with a range of unique ecosystem services that support ecosystem and human health. This paper draws on existing green space planning literature to argue that the integration of community gardens into standardised and previously under-utilised public park landscapes represents an innovative approach to providing ecosystem services. Particular focus is given to the challenges facing green space planning in Perth, Western Australia. At an individual level, community gardens provide a venue for an alternative and more accessible form of physical activity – gardening – and a restorative park environment that is a more attractive destination for neighbourhood walking. At the community level, gardens can facilitate bridging interactions between different social groups, whilst providing opportunities for local residents to participate actively in green space planning processes. Perhaps most importantly, community gardens can provide unique opportunities for environmental education that lead to enhanced local ecological outcomes. The paper concludes with a brief overview of the main challenges likely to be faced with this integration, and some strategies that may allow them to be overcome. It is hoped this paper will provide a background for future case studies, and a catalyst for increasing integration between formal green space planning and community garden development.  相似文献