Application of five DNA marker techniques to distinguish between five apple (Malus × domestica Borkh.) cultivars and their sports     

Anita Kuras  Kristina Antonius  Ruslan Kalendar  Dorota Kruczy ´nska 《The Journal of Horticultural Science and Biotechnology》2013,88(6):790-794

Summary

Genetic variation between five apple cultivars (‘Golden Delicious’, ‘Gala’, ‘Jonagold’, ‘?ampion’, and ‘Idared’) and ten of their sports (‘Golden Delicious Reinders’, ‘Goldrosio’, ‘Gala Must’, ‘Gala Schniga Schnitzer’, ‘Jonagored’, ‘Jonagold Excel’, ‘Szampion Arno’, ‘Szampion Reno Malinowy’, ‘Idaredest’, and ‘Red Idared’) was investigated using five types of DNA markers: Inter-Simple Sequence Repeats (ISSR), Simple Sequence Repeats (SSR), Amplified Fragment Length Polymorphism (AFLP), Sequence-Specific Amplified Polymorphism (S-SAP), and Inter-Primer Binding Site (iPBS) amplification. In total, 941 polymorphic amplified fragments were obtained using 12 ISSR, 12 SSR, ten AFLP, 19 iPBS, and 15 S-SAP primers or primer pairs. Four of the above-described techniques (except for SSRs with the primer pairs used in this study) were able to distinguish between the sports and their parental cultivar. The most effective technique to distinguish between the genotypes analysed was S-SAP, which detects variations in DNA regions flanking retrotransposon insertion sites.The combined use of ISSR,AFLP, iPBS, and S-SAP markers identified and distinguished all of the sports tested.  相似文献   

Drought in the forest breaks plant–fungi interactions     

Boczoń  Andrzej  Hilszczańska  Dorota  Wrzosek  Marta  Szczepkowski  Andrzej  Sierota  Zbigniew 《European Journal of Forest Research》2021,140(6):1301-1321

European Journal of Forest Research - Drought in the forest is not only a prolonged state of water shortage, but also an occasion where interactions between plants and fungi are affected. Water...  相似文献   

Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu)     

Marta Kuchta‐G&#x;adysz  Ewa Wjcik  Dorota S&#x;onina  Anna Grzesiakowska  Agnieszka Otwinowska‐Mindur  Olga Szeleszczuk  Piotr Niedba&#x;a 《Animal Science Journal》2020,91(1)

Cytogenetic tests are used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping predict possible genetic consequences in animal cells and assess animal resistance. The aim of this study was to examine, using cytogenetic tests, the spontaneous chromosome and DNA damage in coypu lymphocytes. Four tests: fragile site (FS), bleomycin (BLM), micronucleus, (MN) and comet were used for the first time in coypu cells. The averages with standard deviations obtained in the research were as follows: 3.30 ± 0.80 fragile sites/cell; 0.63 ± 0.80 BLM damage/cell; 6.10 ± 0.53% binucleated cells with MN; and 3.24 ± 0.63% DNA in tail. The present analysis showed high interindividual variation in spontaneous chromosomal and DNA damage levels. In the case of micronucleus, fragile sites, and comet assays, the differences between animals were statistically significant. The data suggest that these assays are sensitive enough to detect some effects on an individual animal and can be proposed as tools for coypu biomonitoring.  相似文献   

Pregnancy-induced changes in the transcript levels of prolactin receptor and its suppressor in the ovine hypothalamus and adenohypophysis     

Malgorzata Szczesna  Katarzyna Kirsz  Tomasz Misztal  Dorota A. Zieba 《Reproduction in domestic animals》2020,55(1):21-28

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Differences in Cytoplasmic Maturation Between the BCB+ and Control Porcine Oocytes Do Not Justify Application of the BCB Test for a Standard IVM Protocol     

Piotr PAWLAK  Ewelina WARZYCH  Agnieszka CHABOWSKA  Dorota LECHNIAK 《The Journal of reproduction and development》2014,60(1):28-36

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Correlations among antral follicular echotexture,apoptosis and expression of key steroidogenic enzymes in sheep     

Taylor VANDUZER  Raj DUGGAVATHI  Maciej MURAWSKI  Dorota A. ZIEBA  Patrycja SROKA  Pawel M. BARTLEWSKI 《The Journal of reproduction and development》2014,60(6):476-482

Nineteen cycling ewes underwent transrectal ultrasonography of ovaries followed by ovariectomies during the growth phase of the first follicular wave of the interovulatory interval or the proestrus/estrus phase of the cycle. Quantitative ultrasonographic characteristics of the antrum and follicular wall in a total of forty-three ovine antral follicles were examined for correlations with the protein expression of three steroidogenic enzymes (cytochrome P450 17α-hydroxylase, CYP17; cytochrome P450 aromatase, CYP19; and 3β-hydroxysteroid dehydrogenase, 3β-HSD) determined by densitometric analysis of immunohistochemical slides, follicular dimensions, granulosa layer thickness and the percentage of apoptotic granulosa cells. Significant correlations were found between echotextural attributes of ovine antral follicles and the percentage of apoptotic granulosa cells, CYP17 expression (theca), CYP19 expression (granulosa) and 3β-HSD expression (theca cells). Computer-aided analyses of ultrasonographic images can be beneficial to the development of assisted reproductive technologies and diagnosis of hormonal imbalances without the need for ovarian biopsies or hormone assays.  相似文献   

Oak natural regeneration and conversion processes in mixed Scots pine stands     

Dobrowolska  Dorota 《Forestry》2006,79(5):503-513

The main task of the study was to find out if spontaneous oakregeneration can play an important role in conversion processesof mixed Scots pine stands to native broadleaved forests. Thepaper examines the amount and structure of oak natural regenerationunder different gap sizes and defines the optimal gap size forinitiation, growth and development of oak regeneration. Theinvestigation was conducted in managed forests in the GarwolinForest District in the central part of Poland. All gaps, definedas openings in the canopy  相似文献   

Evaluation of sperm morphometry of rabbits (Oryctolagus cuniculus f. domesticus)     

Olga Szeleszczuk  Katarzyna Andraszek  Dorota Banaszewska  Piotr Niedbała  Marta Kuchta-Gładysz  Anna Grzesiakowska 《Reproduction in domestic animals》2021,56(3):408-415

Sperm morphology and morphometry are considered parameters in fertility diagnosis. They are especially important in the case of species for which there is no standard with respect to morphometric sperm parameters. It is then crucial to apply the staining technique that has the least influence on the sperm structure and provides the most detailed image, so as to enable measurements. The aim of the research was to assess the morphometric parameters of rabbit sperm using silver nitrate staining. The staining process revealed a detailed image of the spermatozoon head and tail, thus enabling precise measurements. From these basic morphometric parameters, four additional shape indices characterizing the sperm head were calculated: ellipticity, elongation, roughness and regularity. These parameters more precisely characterize the shape of the sperm head. Silver nitrate staining can be used as an independent technique in assessment of sperm structure or to supplement routine diagnostics.  相似文献   

Cover cropping influences physico-chemical soil properties under direct drilling soybean     

Elżbieta Harasim  Dorota Gawęda  Marian Wesołowski  Cezary Kwiatkowski  Magdalena Gocół 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(1):85-94

In seeking effective methods to prevent soil degradation, conservation tillage plays an important protective role. Apart from significantly reducing production costs, cover crops contributes to beneficial changes in the soil environment. A three-year field experiment included three cover crops (winter rye, winter oilseed rape, and white mustard) subjected to mulching or desiccation and to the action of a herbicide at three rates (100%, 75%, and 50%). The study evaluated soil moisture and the content of organic matter, phosphorus, potassium, and magnesium in two soil layers (0–15?cm and 15–30?cm). Cover cropping had a positive effect on soil organic matter content. More organic matter (by 4.7%) was recorded in the topsoil layer (0–15?cm). Among the cover crops most favorable effect on the content of organic matter in the soil had white mustard (an increase of 14.2%) compared to the control. Moreover, rye and white mustard mulch increased the soil content of phosphorus and magnesium, while oilseed rape mulch increased the potassium content. At the critical growth stages (the flowering/pod set) of soybean (Glycine max (L.) Merril), soil moisture was dependent on mulching treatment and soil layers.  相似文献   

Managing the Florida Everglades: Changing values, changing policies     

Dorota Z. Haman  Mark Svendsen 《Irrigation and Drainage Systems》2006,20(2-3):283-302

The Florida Everglades is a ecologically rich land and water environment that has gone through three phases. In the first phase, a small indigenous population drew its low-impact livelihood from the Everglades. During the second phase, the Everglades was drained, plowed, developed, and seriously damaged, while helping to support a booming South Florida economy. During the third phase, earlier alterations of water flow patterns in a part of the original Everglades are being restored and agricultural impacts are being mitigated.  相似文献