Molecular and serological characterization of iris yellow spot virus, a new and distinct tospovirus species   总被引：1，自引：0，他引：1  

Cortês I  Livieratos IC  Derks A  Peters D  Kormelink R 《Phytopathology》1998,88(12):1276-1282

ABSTRACT A new tospovirus was identified in iris cultivations in the Netherlands. Both serological comparisons and sequence determination of the S RNA demonstrate that this virus represents a new and distinct species, belonging to a separate serogroup, and for which the name iris yellow spot virus (IYSV) is proposed. The disease symptoms on iris are characterized by yellow spots on the leaves. Its experimental host range is very narrow and, in addition to iris, only includes Nicotiana benthamiana and Datura stramonium. The nucleoprotein of IYSV shows only 30 to 44% sequence identity with those of other tospoviruses identified so far; the highest homology being found with the tospovirus species of serogroup IV.  相似文献   

Spoligotype diversity and 5-year trends of bovine tuberculosis in Extremadura,southern Spain     

Waldo L. García-Jiménez  María Cortés  José M. Benítez-Medina  Inés Hurtado  Remigio Martínez  Alfredo García-Sánchez  David Risco  Rosario Cerrato  Cristina Sanz  Miguel Hermoso-de-Mendoza  Pedro Fernández-Llario  Javier Hermoso-de-Mendoza 《Tropical animal health and production》2016,48(8):1533-1540

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The legacy of Columbus in American horse populations assessed by microsatellite markers          下载免费PDF全文

O. Cortés  S. Dunner  L.T. Gama  A.M. Martínez  J.V. Delgado  C. Ginja  L.M. Jiménez  J. Jordana  C. Luis  M.M. Oom  D.P. Sponenberg  P. Zaragoza  Biohorse Consortium  J.L. Vega-Pla 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2017,134(4):340-350

Criollo horse populations descend from horses brought from the Iberian Peninsula over the period of colonization (15th to 17th century). They are spread throughout the Americas and have potentially undergone genetic hybridization with other breeds in the recent past. In this study, 25 autosomal microsatellites were genotyped in 50 horse breeds representing Criollo populations from 12 American countries (27 breeds), breeds from the Iberian Peninsula (19), one breed each from France and Morocco and two cosmopolitan horse breeds (Thoroughbred and Arabian). The genetic relationships among breeds identified five clusters: Celtic; Iberian; North American with Thoroughbred influence; most Colombian breeds; and nearly all other Criollo breeds. The group of “all other Criollo breeds” had the closest genetic relationship with breeds originating from the Iberian Peninsula, specifically with the Celtic group. For the whole set of Criollo breeds analysed, the estimated genetic contribution from other breeds was approximately 50%, 30% and 20% for the Celtic, Iberian and Arab-Thoroughbred groups, respectively. The spatial distribution of genetic diversity indicates that hotspots of genetic diversity are observed in populations from Colombia, Ecuador, Brazil, Paraguay and western United States, possibly indicating points of arrival and dispersion of Criollo horses in the American continent. These results indicate that Criollo breeds share a common ancestry, but that each breed has its own identity.  相似文献   

Identification of Corynebacterium pseudotuberculosis isolated from muscular abscesses in two horses: First report in Mexico          下载免费PDF全文

A. V. Muñoz‐Bucio  Y. A. Cortés‐Pérez  B. Arellano‐Reynoso  M. Hernández‐Gil  R. Hernández‐Castro  E. Díaz‐Aparicio 《Equine Veterinary Education》2017,29(8):431-435

Two Quarter Horse stallions from the same region in Mexico presented with pectoral abscesses and lameness. Exudate samples were taken from drained muscular abscesses and Corynebacterium pseudotuberculosis, identified by bacteriological techniques and multiplex polymerase chain reaction, was obtained in a pure isolation culture from both samples. The sequences of a hypervariable region of the rpoB gene showed 100% and 99% identity to C. pseudotuberculosis 316 biovar equi (CP003077.1) isolated from a horse. Both strains' sequences were located in the same phylogenetic cluster as other biovar equi strain sequences obtained from GenBank. Both horses responded satisfactorily to treatment, which included surgical procedures and wound management. These study cases proved the infection of Mexican horses with C. pseudotuberculosis; however, the prevalence of this pathogen remains unknown.  相似文献   

Mobilization of energetic reserves during starvation in juveniles of different size of the redclaw crayfish Cherax quadricarinatus          下载免费PDF全文

N.S. Calvo  L. Stumpf  E. Cortés‐Jacinto  F. Castillo Díaz  L.S. López Greco 《Aquaculture Nutrition》2018,24(3):952-960

Mobilization patterns of energy reserves during starvation are highly variable among crustaceans, and understanding this process is useful to satisfy the nutritional requirements of cultured organisms. The aim of this study was to elucidate the mobilization patterns on early and advanced juveniles—first free stage and one‐gram juveniles—of redclaw crayfish (Cherax quadricarinatus). A biochemical approach was used to analyse the organic carbon, total proteins, lipids and glycogen levels of the entire animal, and a histochemical approach was adopted to identify location of metabolites in the hepatopancreas and abdominal muscle. While starvation did not affect early juvenile protein levels, it showed a significant decrease in advanced juveniles. Histochemical analysis showed that lipid storage of hepatopancreas R cells was depleted near point of no return (PNR) and recovered after feeding. Glycogen storage was localized in the F cells of the hepatopancreas, and among muscle fibres of the abdomen, where after feeding, a pronounced accumulation was observed. Early and advanced juveniles of redclaw crayfish showed different patterns of consumption mainly related to the protein level, which was the most abundant reserve in advanced juveniles and the most consumed during starvation. Hepatopancreas R cells were confirmed as the principal lipid storage, whereas the abdominal muscle was the main glycogen storage. In crustacean aquaculture, the understanding of resistance to temporary feed deprivation is relevant to achieve an adequate feeding management, thereby avoiding the overfeeding and diminishing operating costs.  相似文献   

Comparison of genotyping by sequencing and microsatellite markers for unravelling population structure in the clonal fungus Verticillium dahliae          下载免费PDF全文

V. Rafiei  Z. Banihashemi  R. M. Jiménez‐Díaz  J. A. Navas‐Cortés  B. B. Landa  M. M. Jiménez‐Gasco  B. G. Turgeon  M. G. Milgroom 《Plant pathology》2018,67(1):76-86

Microsatellite genotyping of a large sample of isolates of Verticillium dahliae from diverse locations recently identified seven distinct genotypic clusters. However, these clusters were not put in the context of phenotypes known to be correlated with clonal lineages in V. dahliae. The objective of this study was to compare clusters defined by microsatellite markers with clonal lineages defined by single‐nucleotide polymorphisms (SNPs) and vegetative compatibility groups (VCGs). Genotyping isolates known to belong to specific clonal lineages (based on SNPs) with microsatellite markers determined the correspondence of clusters and lineages. All but one cluster corresponded to a known clonal lineage, allowing analysis of correlations of phenotypes with microsatellite genotypes from other studies. As shown previously, most race 1 isolates are in lineage 2A, and most isolates with the defoliating pathotype are in lineage 1A. Phylogenetic incompatibility was used to test for recombination or homoplasy caused by hypervariable microsatellite loci; incompatibility was highly correlated with the number of alleles per locus, suggesting that homoplasy caused by parallel evolution of microsatellite alleles is the cause of incompatibility. Microsatellite genotyping of lineage 1A isolates from cotton and olive in Spain over a 29‐year period revealed remarkably little variation; these markers did not mutate enough to provide insight on the spatial and temporal expansion of this clone. Overall, this study showed that microsatellite genotyping can be used to identify clonal lineages in V. dahliae, which has predictive power for inferring phenotypes of phytopathological relevance such as race and pathotype.  相似文献   

Spermatophore production and sperm quality of the river prawn Macrobrachium americanum Spence Bate, 1868 fed with different diets     

Juan Carlos Prez‐Rodríguez  Jaime Gmez‐Gutirrez  Laura Susana Lpez‐Greco  Edilmar Corts‐Jacinto 《Aquaculture Research》2019,50(11):3117-3129

The effects of different diets on spermatophore production and sperm quality were investigated in the river prawn Macrobrachium americanum. River prawns were cultured and fed with three diets for 244 days: fresh food (50% squid meat, Dosidicus gigas and 50% sardine muscle, Sardinops sagax); commercial pellets (35 Purina®); and a 50:50 mixture of both diets. Spermatophore production was recorded every 24 days on average as the percentage of spermatophores produced per extraction per diet, weight and biochemical composition. Sperm quality was measured as the total number of sperm, the proportion of live/dead sperm and normal/abnormal sperm morphology. There were no significant differences in the mean biochemical composition of M. americanum spermatophores for any of the diets. Biochemical composition was 36.3% protein, 25.8% carbohydrate and 4.6% lipids for all data pooled. The weight of spermatophores and sperm counts was not significantly different among diets, nor were there any differences as a function of the male initial total length (p > .05). Male river prawn reproductive exhaustion was observed as a decline in spermatophore production, weight of the spermatophores and the number of sperm cells per spermatophore, with an increasing proportion of dead and abnormal sperm seen throughout the experiment. The recommended period of maintenance in captivity for male broodstock is less than 115 days. It is recommended to feed broodstock males of M. americanum with commercial pellets because no significant differences were detected with the diets tested; pellets are easier to use, ensuring the same spermatophore production and sperm quality that was obtained with fresh food.  相似文献   

Cylinder plate assay for lasalocid sodium     

J M Scheiner  W M Cort  J Chen 《Journal of the Association of Official Analytical Chemists》1974,57(4):978-981

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Towards an empirical model to estimate the spatial variability of grapevine phenology at the within field scale     

Verdugo-Vásquez  N.  Acevedo-Opazo  C.  Valdés-Gómez  H.  Ingram  B.  García de Cortázar-Atauri  I.  Tisseyre  B. 《Precision Agriculture》2020,21(1):107-130

Precision Agriculture - The aim of this study is to propose an empirical spatial model to estimate the spatial variability of grapevine phenology at the within-field scale. This spatial model...  相似文献   

Sorption Behavior of 4-Chlorophenol from Aqueous Solutions By a Surfactant-modified Mexican Zeolitic Rock in Batch and Fixed Bed Systems     

Raúl Cortés-Martínez  Marcos Solache-Ríos  Verónica Martínez-Miranda  Ruth Alfaro-Cuevas V. 《Water, air, and soil pollution》2007,183(1-4):85-94

The removal of 4-chlorophenol from aqueous solutions by both a Mexican clinoptilolite-heulandite zeolitic rock and the modified zeolitic material with the surfactant hexadecyltrimethylammonium bromide (HDTMABr), using batch and packed-bed (column) configurations, was investigated. The unmodified zeolitic rock did not show any adsorption of 4-chlorophenol. The effects of pH, contact time and concentration of 4-chlorophenol on the adsorption process by the surfactant modified material were examined. The sorption of 4-chlorophenol was not affected by the pH range from 4 to 9.5. 4-chlorophenol retention reached equilibrium in about 18 h and the rate of 4-chorophenol adsorption by the modified material was faster in the first 10 h than later. The experimental data were treated with the models: pseudo-first order, pseudo-second order, fractional power and Elovich models. Although, the last three gave correlation coefficients higher than 0.96, the pseudo-second order model was the best to describe the reaction rate. The experimental data follow a linear isotherm which is characteristic for sorption of organic solutes by the partition mechanism. The Bed Depth-Service Time Model was applied to the sorption results in order to model the column operation. The results showed that the surfactant modified zeolitic rock could be considered as a potential adsorbent for 4-chlorophenol removal from aqueous solutions.  相似文献