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51.
A study of frequency of consumption and familiarity with preparing chestnuts was conducted in the state of Missouri from 2003 to 2008. A conjoint analysis of chestnut attributes was completed in 2008 to expand on previous consumer preference research. Information from final consumers was collected during the annual Missouri Chestnut Roast festival at the Horticulture and Agroforestry Research Center in New Franklin, Missouri. The study tracks changes in frequency of consumption and consumer familiarity with preparing chestnuts. The conjoint analysis evaluated pair-wise profiles to quantify the effects of origin, production process and price on consumer preferences. Results show improvements in the frequency of consumption and familiarity with cooking chestnuts in Missouri. Findings suggest the festival has influenced consumption patterns among return visitors. The conjoint analysis identified locality of produce and organic certification to be major drivers behind consumer preferences. Price is also an important attribute influencing consumer preferences. Conjoint analysis results indicate that chestnuts grown in Missouri or the U.S. carrying an organic label can capture price premiums.  相似文献   
52.
In the present study, the performance of neuronal networks models in monthly landing forecasting of jack mackerel (Trachurus murphyi) in central‐southern Chile (32°S–42°S) was assessed. Thus, monthly estimations for 10 environmental variables, fishing effort (fe) and jack mackerel landings for the period 1973–2008 were used. A preliminary analysis was done in order to remove strongly correlated variables. Sea surface temperature (SST) and fe are established as input variables, then, a non‐linear cross correlation analysis was performed to estimate the lag between the input variables and jack mackerel landings. Two models were adjusted: model one includes both training and testing cases randomly selected using all data involved in the analysed period; for model 2, the data is divided into two time series: the first from 1973 to 2002 used for training and the second between 2003 and 2008 used for validation. The external validation process for model 1 showed an explained variance of 92%, with a standard forecasting error of 30%. The explained variance for model 2 was 81%, with a standard forecasting error of 38%. Finally, the sensitivity analysis for both models showed the fe as the most influential variable to jack mackerel landings, which presents functionality depending on anthropogenic effects rather than environmental conditions.  相似文献   
53.
Piaractus mesopotamicus juveniles (total length 12 ± 0.5 mm) were exposed to different concentrations of ammonia‐N (un‐ionized plus ionized ammonia as nitrogen), using the static renewal method at different temperature levels (15, 20 and 25°C) at pH 7. The 24, 48, 72, 96 h LC50 values of ammonia‐N in P. mesopotamicus juveniles were 5.32, 4.19, 3.79 and 2.85 mg L?1 at 15°C; 4.81, 3.97, 3.25 and 2.50 mg L?1 at 20°C; and 4.16, 3.79, 2.58 and 1.97 mg L?1 at 25°C respectively. The 24, 48, 72, 96 h LC50 values of NH3‐N (un‐ionized ammonia as nitrogen) were 0.018, 0.014, 0.013, 0.009 mg L?1 at 15°C temperature; 0.023, 0.019, 0.016 and 0.012 mg L?1 at 20°C; 0.029, 0.026, 0.018 and 0.014 mg L?1 at 25°C. The temperature increase from 15 to 25°C caused an increase of ammonia‐N susceptibility by 21.80%, 9.55%, 31.92% and 30.87%, after 24, 48, 72 and 96 h exposure respectively. Furthermore, we found that exposure of fish to ammonia‐N caused an elevation in total haemoglobin and blood glucose with an increase of 2 mg L?1 concentration. Ammonia levels tolerated, especially in different temperatures levels, have important implications for the management of aquaculture.  相似文献   
54.
Fishing tourism is growing steadily in Brazil. Artisanal fishers meeting the demand for recreational fishing are capturing live juvenile shrimp in the estuaries and selling them for live bait, preventing the recruitment of a larger contingent into the ocean. This study compares the efficiency of using lambari, Deuterodon iguape Eigenmann, as live bait with the shrimp, Penaeus schmitti Burkenroad, while fishing for common snook (Centropomus spp.), as well as to evaluate the swimming behaviour of D. iguape in different salinities. The results showed that there was no significant difference between the live baits used (P. schmitti vs D. iguape). The month, the period of the day and salinity presented differences. The size of the species did not depend on the type of bait used. There was no statistical difference in swimming behaviour, but there was a trend of increased speed and displacement in the group exposed to a salinity of 10 g/L. In conclusion, D. iguape appears as effective as P. schmitti and can be successfully used as live bait; and from an ecological point of view, it reduces the impact of fishing on juvenile shrimp.  相似文献   
55.
Only with new technology and the ability to simulate systems are we beginning to understand fish reproductive processes at the individual scale over time. Traditionally, oocyte recruitment and fecundity type in fishes have been based on a “snapshot” approach, that is a histological micrograph and/or oocyte size frequency distribution of a spawning capable female has been assumed to indicate oocyte recruitment processes over time. This has important implications as the asynchronous and group‐synchronous oocyte recruitment patterns are associated with indeterminate and determinate fecundity types, respectively, and thus affect estimates of reproductive potential and spawning biomass (if using egg production methods). However, a historic review of these terms revealed that they are not applied consistently, with the same oocyte recruitment pattern often assigned to both of these by different scientists. The intent of this study is to develop a conceptual model of oocyte recruitment processes over time, key drivers, and resulting “snapshots” to refine oocyte recruitment terms, definitions, and the methods needed to identify them.  相似文献   
56.
There is considerable interest in finding replacements for the synthetic fungicides and preservatives that are currently used to control fungal pathogens; however, any alternative compounds must be safe and must prevent the development of microbial resistance. In this regard, essential oils have received special attention. Protium colombianum Cuatrecasas is an endemic tree found in tropical rainforests and possesses aromatic and resinous characteristics. To date, there have been no reports concerning the chemistry of this species, which belongs to a genus that represents an interesting source of essential oils that are occasionally used as antimicrobial agents. Therefore, the chemical composition of the essential oils found in P. colombianum collected from two life zones over a five-month period was analyzed using gas-chromatography/mass-spectrometry. A total of 92 components, comprising 97.7–99.9 % of the total composition, were identified based on their mass spectra and the retention indices. Precedence markers were identified for each life zone, and seasonality affected the samples from one life zone when rainfall was maximum. The in vitro activity against Fusarium oxysporum isolates from carnation and chrysanthemum, as well as a Fusarium solani isolate from chrysanthemum, was tested by determining the minimum inhibitory concentration. Assays using essential oils and control substances showed that terpenoid and phenolic compounds are mainly responsible for the observed anti-fungal activity. These results suggest that P. colombianum essential oils exhibit cytotoxicity against Fusarium isolates and that the search for new sources of anti-fungal essential oils should include sources that contain different ratios of terpenoid and phenolic compounds.  相似文献   
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A sensor responsive to Hg2+ (pDL20) was constructed by fusing part of a narrow spectrum mer operon, including its regulatory elements, to promoterless lux genes. This was compared with another mer::lux fusion (pRB28) in order to estimate the available Hg in moss, a conventional bioindicator used for surveying Hg pollution in terrestrial environments. Hg(II) was measured as the relative luminescence unit (RLU) emitted by E. coli cells carrying either pRB28 or pDL20. The linearity ranges of standard curves were measured by spiking HgCl2 at different nanomole levels in a phosphate buffered solution (PBS). The level of correlation between RLU and spiked HgCl2 depended on the incubation time of E. coli cells: the correlation factors (R 2) of the regression lines were highly significant only after 150 min of incubation. pDL20 detected Hg(II) concentrations in the linear range between 0.05 nM and 0.5 nM and was approximately 27 times more sensitive than pRB28. The latter was less sensitive and showed a different range of linearity, from 20 nM to 200 nM. Measurements of bioavailable Hg were performed in buffered solutions leached from moss. Concentrations of Hg(II) were determined by external standard addition of HgCl2. pDL20 was found to be more reliable than pRB28 in the estimation of very low concentrations of bioavailable Hg (II). Both sensors were unable to determine Hg(0) emitted by geothermal activities.  相似文献   
60.
A field experiment was carried out on maize (Zea mays, L.) to study the effects of different fertilizer management on nitrogen status in soil and plant response. Three different fertilizers, mineral (MN), mineral plus buffalo manure (MN + BM) and organo-mineral with peat (OMP), were added at the usual (140, 61 and 116 kg ha−1) and the reduced (70, 31 and 58 kg ha−1) rates of N, P and K. respectively. Soil samples were analyzed for N by both the Kjeldahl method and the electro-ultrafiltration technique (EUF). The soil Kjeldahl-N concentrations were scarcely affected by the different fertilizer treatments, while the EUF-N concentrations were closely correlated with the amounts of N added. The EUF also discriminated between the NO3-N and the sum of the ammonium and the easily extractable organic N forms (EUF-Norg + NH4). The largest proportions of EUF-Norg + NH4 were found in the untreated plots and in the plots treated with buffalo manure. The different fertilizer treatments significantly affected grain yield, which ranged from a minimum of 6.3 t ha−1 from the untreated plots, to a maximum of 11.9 t ha−1 from those supplied with 140 kg N, 61 kg P and 116 kg K ha−1 by OMP fertilizer. The highest agronomic efficiency index for N was exhibited in the OMP treatment at the reduced rate. The grain yield was closely correlated with the total extractable EUF-N, but different relationships were found between the rate of N added, the level of EUF-NO,-N in soil and grain yield for the different fertilizer treatments.  相似文献   
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