Detection of Zoonotic Protozoa Toxoplasma gondii and Sarcocystis suihominis in Wild Boars from Spain          下载免费PDF全文

R. Calero‐Bernal  J. E. Pérez‐Martín  D. Reina  F. J. Serrano  E. Frontera  I. Fuentes  J. P. Dubey 《Zoonoses and public health》2016,63(5):346-350

Food safety regulations require the control of the presence of protozoa in meats destined for human consumption. Wild boar (Sus scrofa) meat may constitute a source of zoonoses. A 23.8% (688/2881) seroprevalence of anti‐Toxoplasma gondii antibodies and 72.2% (662/910) Sarcocystis sarcocysts prevalence were detected among wild boars hunted in Southwestern areas of Spain. Identity of Sarcocystis spp. was performed by RFLP‐PCR and sequencing, detecting S. miescheriana (7/8) and the zoonotic S. suihominis (1/8). Risk assessment studies of these coccidian in meats destined to human consumption are needed.  相似文献   

PCR evaluation of selected vector‐borne pathogens in dogs with pericardial effusion          下载免费PDF全文

M.‐D. Tabar  R. Movilla  L. Serrano  L. Altet  O. Francino  X. Roura 《The Journal of small animal practice》2018,59(4):248-252

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The application of polyamines by pressure or immersion as a tool to maintain functional properties in stored pomegranate arils     

Mirdehghan SH  Rahemi M  Serrano M  Guillén F  Martínez-Romero D  Valero D 《Journal of agricultural and food chemistry》2007,55(3):755-760

Pomegranate fruits were treated with putrescine (Put) or spermidine (Spd) at 1 mM either by pressure infiltration or by immersion and then were stored at 2 degrees C for 60 days. Samples were taken biweekly and were further stored 3 days at 20 degrees C for shelf life study. The treatments were effective on maintaining the concentration of ascorbic acid, total phenolic compounds, and total anthocyanins in arils at higher levels than in control samples. In addition, the two ways of polyamine application increased the levels of total antioxidant activity (TAA) during storage, especially when polyamines were applied by pressure infiltration. Moreover, Spd showed the best results on increasing TAA through maintenance of total phenolic compounds.  相似文献   

Dietary fiber content and associated antioxidant compounds in Roselle flower (Hibiscus sabdariffa L.) beverage     

Sáyago-Ayerdi SG  Arranz S  Serrano J  Goñi I 《Journal of agricultural and food chemistry》2007,55(19):7886-7890

The beverage of Hibiscus sabdariffa flowers is widely consumed in Mexico. Polyphenols contained in plant foods are frequently associated with dietary fiber. The aim of this work is to quantify the dietary fiber, associated polyphenols, and antioxidant capacity of the Roselle flower and the beverage traditionally prepared from it and its contribution to the Mexican diet. Roselle flower contained dietary fiber as the largest component (33.9%) and was rich in phenolic compounds (6.13%). Soluble dietary fiber was 0.66 g/L in beverage, and 66% of total extractable polyphenols contained in Roselle flower passed to the beverage and showed an antioxidant capacity of 335 micromoL trolox equivalents/100 mL beverage measured by ABTS. These data suggest that Roselle flower beverage intake in the Mexican diet may contribute around 166 and 165 mg/per serving to the intake of dietary fiber and polyphenols, respectively. The health benefits from consumption of Hibiscus beverage could be of considerable benefit to the whole population.  相似文献   

Sex-difference in the ossification rate of the appendicular skeleton in Capra pyrenaica Schinz, 1838, and its utility in the sex identification of long bones     

Serrano E  Pérez JM  Christiansen P  Gállego L 《Anatomia, histologia, embryologia》2006,35(2):69-75

The main goal of our work is to quantify differences in the rate of ossification in post-cranial Iberian ibex skeletons, related to sex. Another objective is to improve criteria for assessing the sex of post-cranial bones by combining the degree of ossification of the distal epiphysis and biometrical data. Forty Capra pyrenaica skeletons were examined in order to determine the degree of ossification by means of an Ossification Index. Our results evidence that sexual differences in the rate of ossification become visible at 6 months of age. On average, females complete their bone development 2 years before males do. Finally, by means of lineal classification functions which take into account both biometrical and anatomical criteria, we can achieve, in average, a 95.5% of correct sex discrimination within a sample consisting of ibex metacarpi and metatarsi from individuals aging from <1 to 12 years. Therefore, we conclude that the rhythm of ossification in the post-cranial skeleton of C. pyrenaica may be used as a criterion for assessing the sex of skeletal remains and could be applicable to other dimorphic ungulates. Nevertheless, the results obtained for specimens belonging to a particular population may have limited application to other populations with different medium sizes and living at particular densities within habitats with variable quality.  相似文献   

Susceptibility to Phytophthora cinnamomi of the commonest morphotypes of Holm oak in southern Spain     

M. S. Serrano  P. De Vita  M. D. Carbonero  F. Fernández  P. Fernández‐Rebollo  M. E. Sánchez 《Forest Pathology》2012,42(4):345-347

The four main morphotypes of Holm oak (Quercus ilex subsp. ballota) present in Andalusia (expansa, macrocarpa, microcarpa and rotundifolia) were infected with Phytophthora cinnamomi to determine their susceptibility to the root pathogen. No large differences were found among the four morphotypes in the infection of roots, which always showed a high degree of necrosis. However, the different responses of the foliage to infection separated the four morphotypes of Holm oak into three groups: very susceptible (microcarpa), susceptible (expansa) and moderately susceptible (rotundifolia and macrocarpa). The natural hybrid Q. ilex ballota–Q. faginea exhibited a low level of root and foliar symptoms when infected with P. cinnamomi. Quercus faginea could be considered as a source of resistance to P. cinnamomi in future breeding programmes.  相似文献   

Anomalous Pregnancies during Late Embryonic/Early Foetal Period in High Producing Dairy Cows     

B Serrano  F López-Gatius  RHF Hunter  P Santolaria  I García-Ispierto  G Bech-Sabat  NM de Sousa  JF Beckers  JL Yániz 《Reproduction in domestic animals》2009,44(4):672-676

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations. Data derived from a large-scale ultrasound pregnancy diagnosis programme in high producing dairy cows. Over a 3-year period (2004–2007), a very low incidence (0.5%: 15 of 3094) of anomalous pregnancies was recorded. The results revealed that the following anomalies were detected on days 35–41 of gestation in cows carrying singletons with one single corpus luteum: embryo death in eight cows (0.3%); and embryo in the uterine horn contralateral to the corpus luteum in seven cows (0.2%). All these animals suffered pregnancy loss during the early foetal period. In cows carrying dead embryos, no signs of conceptus degeneration were observed on pregnancy diagnosis. Amnion size (approximately 25 mm diameter) and uterine horn fluid contents were estimated to be similar to those of the normal pregnant cows in this period. In the contralateral gestations, live embryos were observed in all ultrasound checks before pregnancy loss. Uterine fluid contents increased in the two cows in which gestation continued for more than a week. In the cases of embryo death but not in those of contralateral gestation, a drop in PAG-1 levels was noted prior to pregnancy loss. Two cows carrying dead embryos increased with time allantoic fluid contents. The PAG-1 values increased with time in one cow bearing a dead embryo (from 2.31 to 6.79 ng/ml) and in two of the contralateral gestations (from 1.66 to 2.33 ng/ml and from 0.39 to 6.79 ng/ml, respectively). Results of this study indicate that the foetal membranes continue to undergo some activity following embryo death, and that contralateral pregnancy may determine failure of the gestation process.  相似文献   

Calcium fertilizers induce soil suppressiveness to <Emphasis Type="BoldItalic">Phytophthora cinnamomi</Emphasis> root rot of <Emphasis Type="BoldItalic">Quercus ilex</Emphasis>     

María Socorro Serrano  Paolo De Vita  Pilar Fernández-Rebollo  María Esperanza Sánchez Hernández 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(2):271-279

Based on the observation that the root disease caused by P. cinnamomi on Q. ilex has a low incidence and severity in soils with medium-high Ca²⁺ content, we studied the ability of Ca²⁺ fertilizers to induce soil suppressiveness to the pathogen. Studies on cultures of P. cinnamomi exposed to different Ca²⁺ fertilizers in vitro showed significant inhibition of sporangial, chlamydospore and zoospore production at millimolar concentrations while mycelial growth was mainly unaffected. Experiments performed with artificially infested soil showed that some Ca²⁺ fertilizers induce a significant decrease on chlamydospore viability. Additionally, greenhouse experiments using artificially infested soils showed a significant reduction of foliar and root symptom severities in Holm oak seedlings growing in soils amended with Ca²⁺ fertilizers. We suggest that limestone amendments in oak rangelands could enhance the suppressiveness of soils to P. cinnamomi, and it is likely that the inhibition of sporangial production was the main mechanism involved.  相似文献   

Clinical investigation of a point-of-care blood ammonia analyzer     

Goggs R  Serrano S  Szladovits B  Keir I  Ong R  Hughes D 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(2):198-206

BACKGROUND: Hyperammonemia has frequently been implicated in the pathogenesis of hepatic encephalopathy. Blood ammonia determination requires minimal delay between sampling and analysis for accurate results. OBJECTIVES: The aim of this study was to investigate the PocketChem BA, a new point-of-care (POC) blood ammonia analyzer for clinical use by determining machine precision, linearity, repeatability, and accuracy. METHODS: Coefficients of variation were determined by repeated measurement of 2 control solutions. Linearity was investigated by testing serial dilutions of a stock solution. For accuracy, samples from clinical cases were used to compare the results on the PocketChem BA with those obtained using an enzymatic reference method for canine plasma. Canine and feline patients were consecutively enrolled if blood ammonia was assayed and samples could be analyzed shortly after collection. Classification of results (as normal or high, using 100 micromol/L as a cutoff value), Bland-Altman and Deming regression plots, and intraclass correlation coefficients were used to compare the methods. Stability of samples and test strips also was assessed over time. RESULTS: Coefficients of variation were 10.6% and 4.8% for low and high controls, respectively. Concentrations of ammonia in diluted stock solutions correlated positively with mean measured concentrations (Pearson coefficient 0.988, P<.001). Of the 54 samples obtained from 38 dogs and 4 cats, 41 had ammonia concentrations within the readable range. Results from the POC analyzer and the reference method were correlated positively (intraclass coefficient 0.800, 95% confidence interval 0.655-0.888), with the POC analyzer having negative constant and proportional biases. The methods agreed in the classification of 45/54 (83.3%) samples, with 7 false negative results on the POC analyzer. Results of repeated sample and strip analyses at 1 and 24 hours were significantly different (P<.05) from those at 0 hour. CONCLUSIONS: The PocketChem BA has acceptable precision, adequate linearity, and satisfactory agreement with a reference method, but negative constant and proportional biases. The POC analyzer may be suitable for clinical use in patients suspected of having hepatic encephalopathy, using a lower reference limit of 60 mumol/L to decrease false negative results.  相似文献   

The relevance of preserving temporary ponds during drought: hydrological and vegetation changes over a 16-year period in the Doñana National Park (south-west Spain)     

Laura Serrano  María Zunzunegui 《水产资源保护:海洋与淡水生态系统》2008,18(3):261-279

• 1. Although the Doñana National Park is given the highest degree of environmental protection in Spain, it is likely that groundwater discharge to several ponds within the Biological Reserve has been damaged by abstraction to a tourist resort located less than 1 km away.
• 2. Hydrological changes were monitored over 16 years (1 October 1989 to 30 September 2005) by recording the shallow water table of six temporary ponds at 1–8-week intervals, and the duration of pond wet phase (or hydroperiod) during each hydrological cycle.
• 3. The average rainfall for the study period was 563.2 mm, and included 6 wet, 5 moderate, and 5 dry years in a seemingly random sequence. The average rainy season extended from October until the end of March, while the dry season occupied the rest of the year.
• 4. The water table generally oscillated following this alternation of rainy and dry seasons, but this fluctuation was minimal during dry years, and even failed to occur at some ponds.
• 5. Since 1998/99, the average hydroperiod has shortened by 3 months at Charco del Toro pond, and by almost 2 months at Brezo pond, while the rest of the ponds exhibited a reduction of less than 1 month.
• 6. Vegetation changed in the ponds between May 1990 and 2005. Total plant cover increased (range of increase: 16–65%), and species richness decreased in all ponds (range of species loss: 4–18).
• 7. The reduction in the hydroperiod probably enhanced the growth of a few woody plants to the detriment of flooding-dependent species as the cover of Pinus pinea increased nearly fourfold at Brezo pond, while that of Scirpus lacustris was halved at Charco del Toro pond.
• 8. The pumping area for the nearby tourist resort should be relocated, and a specific management strategy should be developed in order to prevent further damage to the ponds.

Copyright © 2007 John Wiley & Sons, Ltd.  相似文献