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81.
For the use of gene expression for nutritional diagnosis, a rapid and easy method of detecting levels of gene expression was proposed. Agrobacterium tumefaciens carrying the GUS reporter gene was injected into intact tobacco leaves with a plastic syringe, and transient GUS expression was determined. GUS activities were reproducible in young leaves. 相似文献
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以黑龙江省主栽的两个水稻品种为试验材料,通过调查试验材料的生育期、干物质积累、叶面积变化和分蘖消长动态的测定,明确了拔节至抽穗期是水稻产量形成的关键时期;根据试验材料干物质积累的变化规律建立了干物质随时间变化的生长曲线。经检验拟合较好。 相似文献
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Hagiwara Y Fujiwara S Takai H Ohno K Masuda K Furuta T Nakayama H Doi K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):349-351
Pneumocystis carinii pneumonia was diagnosed by postmortem examination of a one-year-old Cavalier King Charles Spaniel with four-week history of dyspnea. Cytologic and histologic examination of lung tissues revealed numerous P. carinii trophozoites and cysts, and P. carinii specific DNA was detected by polymerase chain reaction. The dog showed hypogammagloblinemia and extremely low levels of serum IgG. It was considered that P. carinii pneumonia in this case was associated with an immunodeficient condition which has already been reported in Miniature Dachshunds. 相似文献
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A microtubular mass (MM) defect was found in the spermatozoa of 7 Standardbred stallions; 3 stallions were sons of the same sire. Two of these 3 stallions and 2 other stallions (for a total of 4 out of the 7 stallions) were considered subfertile when the defect was first observed. Fertility improved with time, either during the first breeding season or when a given stallion was used less frequently; however, the MM defect persisted, consisting of tortuous arrays of small abnormal microtubules visible only by transmission electron microscopy. The MM probably contained the protein tubulin as indicated by immunofluorescence. Microtubules of the MM were different from normal microtubules in being highly coiled, rather than straight, without interconnecting bridges or arms and had a 20% smaller cross-sectional diameter than did normal microtubules. Synthesis of the MM may occur near areas of the nuclear membranes normally associated with the manchette of the developing spermatid. 相似文献
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