Physicochemical and Structural Properties of Recovered Elastin from Jumbo Squid (Dosidicus gigas) By-Products     

Hugo E. Ramírez-Guerra  Enrique Márquez-Ríos  Guadalupe M. Suárez-Jiménez  Ofelia Rouzaud-Sández  María E. Lugo-Sánchez  Juan C. Ramírez-Suárez 《Journal Of Aquatic Food Product Technology》2019,28(3):275-286

Physicochemical and structural properties of soluble jumbo squid (Dosidicus gigas) elastin recovered from skin by-products were evaluated. The molecular weight of isolated elastin was ~40 kDa with an isoelectric point (pI) between 9 and 10. Aspartic, glutamic, arginine, proline, glycine, and lysine amino acids were the most abundant in squid elastin, whereas the hydroxyproline absence, ~0.7% cysteine content, and the calculated 0.35 isoleucine/leucine ratio were used as purity index. Total and reactive sulfhydryl contents were similar (247.0 ± 5.1 vs 242.0 ± 7.5 μmol mg^?1 of protein, P ≥ 0.05) in purified squid elastin but surprisingly higher than previously reported in other elastins. On the other hand, the secondary structures of squid elastin analyzed by Fourier transform infrared spectroscopy (FTIR) were ~45% β-sheets, ~15% α-helices, ~10% β-turns, and ~30% undefined structures. In addition, squid elastin experienced glass transition at 82.01 ± 0.01ºC, denaturation temperature at 110.45 ± 0.64ºC, and aggregation at 197.5 ± 0.23ºC. In conclusion, the prevalence of charged amino acids and pI of squid elastin can facilitate its solubilization in hydrophilic systems, whereas the secondary structure profile and thermostability are desirable features in proteins used for biopolymer designs such as food biofilms or barrier systems.  相似文献   

Internal Migration in Mexico: Consolidation of Urban–Urban Mobility, 2000–2015          下载免费PDF全文

Enrique Pérez‐Campuzano  Guillermo Castillo Ramírez  Mateo Carlos Galindo Pérez 《Growth and change》2018,49(1):223-240

Internal migration has been recognized as the major influence in terms of population redistribution across urban systems, but it is not a homogeneous phenomenon. Within the context of internal rural‐urban migration decline and the negative changes in migratory balances in the metropolitan area of Mexico City, the core of enquiry in this paper is the approach to growth and consolidation of an internal urban–urban migration system in the early twenty‐first century (2000–2015). This process has taken place through two main networks, among metropolitan areas not corresponding to the principal city and among intermediate cities. Internal migration is a complex process that involves both individual and spatial characteristics and which leads to spatially uneven development in the long term. Data from three censuses of the population of Mexico (2000, 2010, and 2015) show a transition to a more urban–urban migration pattern, with skilled migrants tending to have metropolitan and urban destinations, whereas less‐skilled migrants prefer rural and small urban destinations.  相似文献   

Identification of genetic sources with attenuated <Emphasis Type="Italic">Tomato chlorosis virus</Emphasis>-induced symptoms in <Emphasis Type="Italic">Solanum</Emphasis> (section <Emphasis Type="Italic">Lycopersicon</Emphasis>) germplasm     

Matías González-Arcos  Maria Esther de Noronha Fonseca  Ana Arruabarrena  Mirtes F. Lima  Miguel Michereff-Filho  Enrique Moriones  Rafael Fernández-Muñoz  Leonardo S. Boiteux 《Euphytica》2018,214(10):178

The whitefly-transmitted Tomato chlorosis virus (ToCV) (genus Crinivirus) is associated with yield and quality losses in field and greenhouse-grown tomatoes (Solanum lycopersicum) in South America. Therefore, the search for sources of ToCV resistance/tolerance is a major breeding priority for this region. A germplasm of 33 Solanum (Lycopersicon) accessions (comprising cultivated and wild species) was evaluated for ToCV reaction in multi-year assays conducted under natural and experimental whitefly vector exposure in Uruguay and Brazil. Reaction to ToCV was assessed employing a symptom severity scale and systemic virus infection was evaluated via RT-PCR and/or molecular hybridization assays. A subgroup of accessions was also evaluated for whitefly reaction in two free-choice bioassays carried out in Uruguay (with Trialeurodes vaporariorum) and Brazil (with Bemisia tabaci Middle-East-Asia-Minor1—MEAM1?=?biotype B). The most stable sources of ToCV tolerance were identified in Solanum habrochaites PI 127827 (mild symptoms and low viral titers) and S. lycopersicum ‘LT05’ (mild symptoms but with high viral titers). These two accessions were efficiently colonized by both whitefly species, thus excluding the potential involvement of vector-resistance mechanisms. Other promising breeding sources were Solanum peruvianum (sensu lato) ‘CGO 6711’ (mild symptoms and low virus titers), Solanum chilense LA1967 (mild symptoms, but with high levels of B. tabaci MEAM1 oviposition) and Solanum pennellii LA0716 (intermediate symptoms and low level of B. tabaci MEAM1 oviposition). Additional studies are necessary to elucidate the genetic basis of the tolerance/resistance identified in this set of Solanum (Lycopersicon) accessions.  相似文献   

Phenolic Compounds,Antioxidant Activity and Lipid Profile of Huitlacoche Mushroom (Ustilago maydis) Produced in Several Maize Genotypes at Different Stages of Development     

Maribel Valdez-Morales  L. Céspedes Carlos  María Elena Valverde  Enrique Ramírez-Chávez  Octavio Paredes-López 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(4):436-443

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Extractants for available boron in limed acid soils and its correlation in crops with high carbon storage capacity (Tectona grandis)     

Jaime L. Mercado  Enrique M. Combatt 《Journal of plant nutrition》2016,39(14):2136-2143

The objective of this work was to evaluate the efficiency of six extractants for available boron in acid soils undergoing to liming, and to correlate the results of soil analysis with foliar response seen in the teak plants in greenhouse conditions. Acid soil samples were collected and their chemical characterization was performed using standard analysis protocols. Later, agricultural lime was added to the experimental units in greenhouse conditions in order to control the soil acidity and these were incubated for 30 days. Six extracts for available boron were evaluated and correlated with response in the plant by foliar analysis. It is concluded that a high dissolution power of boron extractant solution produces a high sensitive in the determination of the changes of boron concentration in the limed acid soil. These methods are seen to be not adequate for the determination of available boron contents at high concentrations.  相似文献   

Infection by a coronatine-producing strain of Pectobacterium cacticidum isolated from sunflower plants in Mexico is characterized by soft rot and chlorosis     

José Humberto Valenzuela-Soto  Luis David Maldonado-Bonilla  Gustavo Hernández-Guzmán  Gabriel Rincón-Enríquez  Norma Angélica Martínez-Gallardo  Enrique Ramírez-Chávez  Ismael Cisneros Hernández  José Luis Hernández-Flores  John Paul Délano-Frier 《Journal of General Plant Pathology》2015,81(5):368-381

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Ex‐post facto analysis of diseases of the Gulf of Mexico′s white shrimp Litopenaeus setiferus     

Rodolfo Enrique del Rio‐Rodriguez  Atahualpa Sosa‐Lopez  Daniel Pech 《Aquaculture Research》2014,45(5):907-912

This study presents an Ex‐Post Facto analysis of diseases of wild juvenile and adults of Litopenaeus setiferus collected from a field survey at the Natural Protected Area of Terminos lagoon, southern Mexico. The objective of the present approach was to determine if sampling site and/or shrimp age were contributing risk factors for disease between juvenile and adult shrimp; if there was a determined period of time in a year cycle when diseases were more critical, and if the analysis would help to decision‐ making considering what population would pose less risk of disease‐carrying when withdrawn for experimental purposes; all under an after‐the‐fact (ex‐post facto) approach. We identified that juvenile shrimp were at more risk of contracting some diseases in the estuarine environment and June, July and August months, were found to be a critical period when colonizing and parasitic diseases maintained a significant high prevalence in the shrimp population. These assumptions may help for decision‐making when wild shrimp have to be withdrawn from their natural environment for research purposes.  相似文献   

A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis     

María Sol Arias  Jos Luis Surez  G.V. Hillyer  Ivn Francisco  Enrique Calvo  Rita Snchez-Andrade  Pablo Díaz  Rubn Francisco  Pablo Díez-Baos  Patrocinio Morrondo  Adolfo Paz-Silva 《Veterinary journal (London, England : 1997)》2009,182(1):73-78

The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy.The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants.  相似文献   

Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab <Emphasis Type="Italic">Neohelice granulata</Emphasis>, During the Pre-Reproductive Period     

Luciana Avigliano  Ivana S. Canosa  Daniel A. Medesani  Enrique M. Rodríguez 《Water, air, and soil pollution》2018,229(2):44

Adult females of the estuarine crab Neohelice granulata were exposed during the 3-month pre-reproductive period (winter) to the herbicide glyphosate, the most used pesticide in Argentina, at three different concentrations (0.02, 0.2, and 1 mg/L, as active ingredient). At the end of the in vivo assay, the body weight gain and the ovarian growth were estimated, the last one in terms of the gonadosomatic index (GSI), the relative proportion of the different kind of oocytes, and their relative size. A decrease in the body weight gain was observed by effect of pure glyphosate, at all concentrations assayed. Although no differences in either the GSI or vitellogenic protein content of the ovary were noted between any glyphosate concentration and control, a higher proportion of reabsorbed vitellogenic oocytes was observed in the ovaries of crabs exposed to glyphosate at 1 mg/L, together with an increased area of previtellogenic oocytes. These effects were confirmed in vitro, at a glyphosate concentration of 0.2 mg/L. In fact, a higher area of previtellogenic oocytes was seen when glyphosate was added to the culture medium containing ovarian tissue, but a significant higher incidence of reabsorbed vitellogenic oocytes was seen only when eyestalk tissue was also added to the vials, suggesting that the secretion of some neurohormone involved in reabsorption is enhanced. The obtained results indicate that glyphosate is able to harm, in the studied species, both somatic and the ovarian growth.  相似文献   

Development of a bioacoustic sensor for the early detection of Red Palm Weevil (Rhynchophorus ferrugineus Olivier)     

Abelardo Gutiérrez  Vicente Ruiz  Enrique Moltó  Gervasio Tapia  Maria del Mar Téllez 《Crop Protection》2010

The Red Palm Weevil (Rhynchophorus ferrugineus Olivier) is one of the worst pests for palms, since it can completely destroy the plant. Its early detection is very difficult and when the action of the pest is discovered, normally it is too late for recovering the plant. Bioacoustic sensors provide a good alternative for early detection of this pest because larvae activity is audible while they excavate tunnels to feed. This work describes the different stages for the development of an original electronic device based on acoustic sensors for early detection of Red Palm Weevil larvae in the interior of palms. This device is based on characteristic frequencies possibly related to their feeding activity. The performed tests have demonstrated that is possible to detect two week old larvae activity studying the sound intensity around 2250 Hz in palms infested only with 5 individuals under controlled environmental conditions. The activity of other insects artificially situated in the interior of palms did not affect the response of the device, possibly because they produce different frequencies (in the range 5–7 kHz). Future work will be aimed at assessing the infestation levels.  相似文献