Drying-rewetting cycles and γ-irradiation effects on enzyme activities of distinct layers from a Quercus ilex L. litter     

Enrique Alarcón-Gutiérrez  Fabio Ziarelli  Stéven Criquet 《Soil biology & biochemistry》2010,42(2):283-290

In a Mediterranean climate, water stress is one of the principal constraints on proper forest ecosystem functioning. Drought influences rates of organic matter degradation by affecting microbial growth and enzyme activities. The objectives of this study were: (i) to evaluate the effect of repeated drying-rewetting cycles on cellulase, alkaline phosphatase and fluorescein diacetate (FDA) hydrolase activities of three distinct Quercus ilex L. litter layers, and (ii) to investigate the effect of these cycles on γ-irradiated litters in order to distinguish the abiotic influence on the fluctuations observed. Results, for all three layers, showed high correlations between litter water content and enzyme activities. Under mesocosm conditions, and using non-sterilized litter samples, cellulase, alkaline phosphatase, and FDA activities significantly decreased or increased during drying or rewetting cycles respectively. Significant differences were also found when evaluating the effect of litter depth on enzyme activities, the intermediate depth (OLv layer) generally being the most active. For γ-sterilized samples, FDA activity still fluctuated with drying-rewetting cycles. Assays showed that pre-humidification of γ-irradiated litter increased FDA activity two-fold in the first 30 min. All these results have shown that, following drying-rewetting cycles, some of the fluctuations occur independently of microbial growth, suggesting abiotic interactions, such as desorption, in combination with both solvatation status and conformational changes of enzymes.  相似文献   

A Modeling Study of the Impact of a Power Plant on Ground-Level Ozone in Relation to its Location: Southwestern Spain as a Case Study     

Nuria Castell  Enrique Mantilla  Ariel F. Stein  Rosa Salvador  Millán Millán 《Water, air, and soil pollution》2010,209(1-4):61-79

The impact of atmospheric industrial emissions on secondary pollutant formation depends on many factors; one of the most important being the environmental setting in which the industry is located. The environmental setting affects an industry’s impact on ozone levels through both the air mass dispersion (a function of topography and wind patterns) and the emissions of organic volatile compounds (VOC) and nitrogen oxides (NO _x ) in the area. This model-based study shows how the sensitivity of surface ozone changes with the choice of source location. For the analysis, seven points distributed along the Tinto–Guadalquivir Basin (in the Southwestern Iberian Peninsula) were selected. This area is characterized by the close proximity of natural environments and crop fields to cities, roads, and industrial areas with high NO _x emissions. Natural VOC emissions represent more than 60% of the total non-methane volatile organic compounds emitted in the study area. The results reveal that the largest increases in ozone levels are produced when the industry is located both far away from NO _x emission sources and near to biogenic VOC emissions. Furthermore, the highest increases over the hourly and 8-hourly maximums, as well as the highest accumulated daily values, are found in areas characterized by high VOC/NO _x emission ratios and NO _x sensitivity. The study of the recurrent meteorological patterns along with the distribution of chemical indicators of the O₃–NO _x –VOC sensitivity allows the determination of the industry’s geographical impact on ozone levels. This information enables air quality managers to decide the future location of an industry minimizing its impact on smog levels.  相似文献   

Influence of Salinity in the Bioavailability of Zn in Sediments of the Gulf of Cádiz (Spain)     

Inmaculada Riba  Enrique García-Luque  Alejandra Maz-Courrau  María Luisa González de Canales  Tomás Ángel DelValls 《Water, air, and soil pollution》2010,212(1-4):329-336

This study assesses the effect of salinity in bioavailability and toxicity of Zn by means of laboratory bioassays by observing contamination in both sediment and water, accumulation of Zn in biological tissues, and histopathological damage in the gills and guts tissues of Ruditapes philippinarum clams, which were exposed to different types of sediments from the Gulf of Cádiz (SW Spain) as well as two dilutions of toxic mud coming from an accidental mining spill. With this objective, the coefficients of distribution (K _D) for Zn between overlying water and sediments were calculated, the histopathological frequencies in the tissues of the gills and guts of clams were determined, and the biota-sediment bioaccumulation factors as well as the bioaccumulation factors were quantified in the different stations. Results showed that the greatest histopathological damages appeared when the salinity values decreased. Statistical results showed that salinity was inversely correlated with histopathological damage (p?<?0.01) for the lesion index for gills. The most outstanding results were observed in the two dilutions of toxic mud (0.3% and 7.9%) at a salinity value of 10. Salinity was inversely correlated with the concentration of Zn in biological tissues (p?<?0.05) and inversely correlated with the concentration of Zn in water and sediment. Zn mobilization to the overlying water is produced when salinity values decrease.  相似文献   

商用鸡免疫系统的评估     

Enrique Montiel 《中国家禽》2002,24(2):10-13

健康的鸡只在正常情况下会有良好的生产性能表现.但是,要养好健康的鸡群,则有赖于许多因素.这些因素包括管理、营养、免疫计划,而最重要的是有赖于免疫系统的健全发育.免疫系统的发育会受到数种病毒及非病毒因素的影响.在病毒方面,传染性法氏囊病病毒(IBDV)、马立克氏病病毒(MDV)、里奥病毒(REO)、鸡传染性贫血病病毒(CAV)、禽白血病病毒(ALV)等数种为引起免疫抑制的主要病毒.  相似文献   

Factorial regression analysis of genotype by environment interaction in ryegrass populations, using both isozyme and climatic data as covariates     

François Balfourier  Jose Alberto Oliveira  Gilles Charmet  Enrique Arbones 《Euphytica》1997,98(1-2):37-46

A sample of 58 natural ryegrass populations (Lolium perenne L.) from the French and Spanish oceanic coast was evaluated for three seasonal growth traits (i.e. spring, autumn and winter vigour traits) in 5 evaluation sites (three in France and two in Spain). This sample showed a high level of genotype (population) × environment (evaluation sites) interactions for the three agronomic traits. A factor regression analysis, using both isozyme frequencies of populations and climatic factors of evaluation sites as covariates, was carried out on a sub-sample of 30 populations in order to explain these interactions. This method succeeded in explaining most population × location interactions by the product of two covariates. For instance, for spring vigour trait, 72.8% of the interaction term could be explained by the use of two covariates: the PGI2-20 allelic frequency of populations and the minimum temperature of the coldest month of evaluation sites. This study shows the advantage of such a method for plant breeders who need to identify promising ryegrass populations for their breeding objectives. A number of genetic and evolutionary hypotheses are also discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Lipid composition of lees from Sherry wine     

Gómez ME  Igartuburu JM  Pando E  Luis FR  Mourente G 《Journal of agricultural and food chemistry》2004,52(15):4791-4794

In this paper, we describe the study and characterization of the lipids from lees of Sherry wine, one of the main byproducts from the wine-making industry in the Jerez/Xeres/Sherry denomination of the origin zone in Jerez de la Frontera, Spain. The lipid content, extractability, classification, fatty acid composition, and its main chemical characteristics have been determined in order to evaluate their potential use as a food or food additive.  相似文献   

Soil macroinvertebrate fauna of a Mediterranean arid system: Composition and temporal changes in the assemblage     

Enrique Doblas-Miranda  Francisco Sánchez-Piñero  Adela González-Megías 《Soil biology & biochemistry》2007,39(8):1916-1925

Temporal variability is a key factor to understand the structure of belowground communities. Seasonal and annual variations are especially relevant in unpredictable desert ecosystems, where macroinvertebrates are poorly known, despite constituting an important group of soil organisms. In the present study, we analyse the composition and temporal (seasonal and annual) variations of soil macroinvertebrates in an arid area of southern Spain. During two years, macroinvertebrates were sampled in litter and belowground levels by means of soil cores. Results show that the assemblage was dominated by arthropods, especially Formicidae and Coleoptera. The assemblage differed between litter and belowground levels. In litter, detritivores dominated the community, while belowground fauna showed a similar proportion of detritivores and herbivores and a low percentage of predators. Litter and belowground assemblages showed seasonal variations in richness, abundance, biomass and composition, although variations were more marked in litter than belowground. Patterns of seasonal variation also differed between the two study years for both litter and belowground invertebrates. The seasonal and annual variability of the assemblage has potentially important implications for community dynamics in the study system, since the changes in species composition and trophic structure of soil invertebrate assemblages may affect species interactions and food web dynamics over time. Therefore, integrating temporal variability is likely to be crucial to understand soil community dynamics and food webs, especially in heterogeneous, variable systems as deserts.  相似文献   

Determination of Ploidy Level and Nuclear DNA Content in Tunisian Populations of Atriplex halimus L.     

Kheiria Hcini  David J. Walker  Sadok Bouzid  Elena González  Nora Frayssinet  Enrique Correal 《Genetic Resources and Crop Evolution》2006,53(1):1-5

Atriplex halimus L. (Chenopodiaceae) (Saltbush) is a perennial species used as a fodder shrub for livestock in arid and semi-arid areas, particularly in North Africa. The aim of this work was to determine whether differences in ploidy level and/or nuclear DNA content exist among populations from widely-separated sites in Tunisia. We determined nuclear DNA content and chromosome numbers for populations of A. halimus from seven different locations (Gabes, Medenine, Tataouine, Monastir, Tunis, Sidi Bouzid, Kairouan). The chromosome counts showed that all the Tunisian populations, plus a population from Eraclea (Italy), were tetraploid (2n = 4x = 36) whereas a population from Cala Tarida (Spain) was diploid (2n = 2x = 18). With respect to nuclear DNA, the 2C DNA content of population Cala Tarida was estimated to be 2.41 pg. There was no significant difference among the tetraploid populations (or among plants within populations), whose 2C DNA content ranged from 4.92 to 4.97 pg.  相似文献   

The Influence of Heavy Metals and Mineral Nutrient Supply on Bituminaria bituminosa     

David J. Walker  M. Pilar Bernal  Enrique Correal 《Water, air, and soil pollution》2007,184(1-4):335-345

With a view to the selection of plants for the re-vegetation of contaminated, semi-arid land, two populations of the perennial species Bituminaria bituminosa (Fabaceae) from the south of Spain were studied: one (“LA”) from a non-contaminated soil and the other (“C2”) from a similar soil having elevated total levels of Pb and Zn (1,112 and 4,249 μg g^?1, respectively). For sand-cultured plants receiving nutrient solution, flow cytometry showed that heavy metals, at the concentrations measured in aqueous extracts from contaminated soils, had only slight genotoxic effects on root tip cell nuclei. Both populations were also grown in both soils, in two pot experiments. In the first, shoot biomass of LA and C2 in the contaminated soil was decreased to similar extents, with respect to the “clean” soil. Tissue heavy metal concentrations were unlikely to have been phytotoxic, except in the case of shoot Zn for population LA, but there were tissue deficiencies of P and K for populations LA and C2, respectively. In the second pot assay, the stimulation of growth by NPK fertiliser confirmed that even though this soil had high total heavy metal levels, nutrient availability was the principal factor limiting growth. The lesser transport of heavy metals (Cd, Mn and Zn) to the shoot by the population from the contaminated site is a factor that should be considered when selecting B. bituminosa lines for the phytostabilisation of such sites.  相似文献   

Phosphorylation networks regulating JNK activity in diverse genetic backgrounds     

Bakal C  Linding R  Llense F  Heffern E  Martin-Blanco E  Pawson T  Perrimon N 《Science (New York, N.Y.)》2008,322(5900):453-456

Cellular signaling networks have evolved to enable swift and accurate responses, even in the face of genetic or environmental perturbation. Thus, genetic screens may not identify all the genes that regulate different biological processes. Moreover, although classical screening approaches have succeeded in providing parts lists of the essential components of signaling networks, they typically do not provide much insight into the hierarchical and functional relations that exist among these components. We describe a high-throughput screen in which we used RNA interference to systematically inhibit two genes simultaneously in 17,724 combinations to identify regulators of Drosophila JUN NH(2)-terminal kinase (JNK). Using both genetic and phosphoproteomics data, we then implemented an integrative network algorithm to construct a JNK phosphorylation network, which provides structural and mechanistic insights into the systems architecture of JNK signaling.  相似文献