Genetic engineering in agriculture     

Colwell RK  Norse EA  Pimentel D  Sharples FE  Simberloff D 《Science (New York, N.Y.)》1985,229(4709):112-113

  相似文献   

Recent developments in the study of energy transfer     

Pimentel GC  Brown HW  Otvos JW 《Science (New York, N.Y.)》1966,151(3712):861-862

  相似文献   

Detecting genetic diversity in diploid bananas using PCR and primers from a highly repetitive DNA sequence     

Robert L. Jarret  Dirk R. Vuylsteke  Nicholas J. Gawel  Reynold B. Pimentel  Lisa J. Dunbar 《Euphytica》1993,68(1-2):69-76

Summary The polymerase chain reaction (PCR) was used to detect polymorphisms among 29 diploid clones of Musa acuminata Colla. from Papua New Guinea. Primer sequences were derived from a 520 bp highly repetitive DNA sequence isolated from M. acuminata ssp. malaccensis. Primers, used individually, detected a total of 48 polymorphisms that were scored as unit characters and used to generate a Jaccard's similarity index. Principal coordinate analysis (PCO) was used to cluster clones and the unweighted paired-group method of analysis (UPGMA) was used to compute genetic distance among the materials examined. The abundance of diversity within the PNG diploids examined reflects the extreme genetic variability within the M. acuminata gene pool. PCR, utilizing primers from a highly repetitive sequence, is a rapid means of detecting genetic diversity in M. acuminata.  相似文献   

Granulomatous meningoencephalitis due to toxoplasma gondii in a black-headed night monkey (Aotus nigriceps)     

Antoniassi NA  Boabaid FM  Souza RL  Nakazato L  Pimentel MF  Filho JO  Pescador CA  Driemeier D  Colodel EM 《Journal of zoo and wildlife medicine》2011,42(1):118-120

This report describes toxoplasmosis infection in a black-headed night monkey (Aotus nigriceps) from the Zoological Park of the Federal University of Mato Grosso, Brazil. The animal was submitted alive to the Veterinary Hospital of the Federal University of Mato Grosso presenting neurologic clinical signs. There were no improvements on the animal's clinical condition after treatment, so it was euthanized. At necropsy, the most significant gross lesions observed were hemorrhages diffusely scattered throughout the cerebral hemispheres. Histologic findings were characterized by nonsuppurative encephalitis and mild multifocal necrotizing nonsuppurative myocarditis. Groups of tachyzoites and cysts were observed surrounding the inflamed areas of cerebral parenchyma. Sections of the brain were submitted for immunohistochemistry examination for Toxoplasma gondii and Neospora caninum and also for polymerase chain reaction (PCR) to determine the presence of Toxoplasma gondii. The samples were positive for Toxoplasma gondii by PCR; however, cross reactivity was observed between Toxoplasma gondii and Neospora caninum antibodies. These findings were consistent with a toxoplasmosis infection in this animal.  相似文献   

Robust QTL fine mapping by applying a quantitative transmission disequilibrium test to the Mendelian sampling term     

H. Simianer  E.C.G. Pimentel 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(6):432-442

In many farm animal populations, high‐density single nucleotide polymorphism (SNP) genotypes are becoming available on a large scale, and routine estimation of breeding values is implemented for a multiplicity of traits. We propose to apply the basic principle of the quantitative transmission disequilibrium test (QTDT) to estimated Mendelian sampling terms. A two‐step procedure is suggested, where in the first step additive breeding values are estimated with a mixed linear model and the Mendelian sampling terms are calculated from the estimated breeding values. In the second step, the QTDT is applied to these estimated Mendelian sampling terms. The resulting test is expected to yield significant results if the SNP is in sufficient linkage disequilibrium and linkage with quantitative trait loci (QTL). This principle is illustrated with a simulated data set comprising 4665 individuals genotyped for 6000 SNP and 15 true QTL. Thirteen of the fifteen QTL were significant on a genome‐wide 0.1% error level. Results for the empirical power are derived from repeated samples of 1000 and 3000 genotyped individuals, respectively. General properties and potential extensions of the methodology are indicated. Owing to its computational simplicity and speed, the suggested procedure is well suited to scan whole genomes with high‐density SNP coverage in samples of substantial size and for a multiplicity of different traits.  相似文献   

High fry production rates using post-thaw silver carp (Hypophthalmichthys molitrix) spermatozoa under farming conditions     

Brbaro Alvarez  Roberto Fuentes  Rafael Pimentel  Zoila Abad  Edenaida Cabrera  Eulogio Pimentel  Amilcar Arenal 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):195-201

Silver carp (Hypophthalmichthys molitrix) is not endemic to Cuba, and egg fertilization is totally artificial; males produce spermatozoa only between March and October. Cryopreservation of silver carp spermatozoa would reduce the number of males needed, minimize handling stress through less frequent stripping, and facilitate artificial propagation when eggs are available. The effects on motility and fry production from eggs fertilized with thawed sperm under farm-conditions were examined in this study. Five, seven and ten percent of dimethyl sulfoxide (DMSO), glycerol and methanol were tested as cryoprotectants. DMSO was a more suitable cryoprotectant than methanol or glycerol. The effect of equilibration time on the motility rate at 10% DMSO was evaluated. Hatching rates equal to the control (P>0.01) were obtained under farm conditions with frozen spermatozoa, stored even for a year in liquid nitrogen, with a final DMSO concentration of 10%. Cryopreservation offers a useful routine method for sperm storage and silver carp handling. To our knowledge, this is the first report on the production of fry from post-thaw silver carp spermatozoa under farming conditions.  相似文献   

Implementation of genetic evaluation and mating designs for the endangered local pig breed ‘Bunte Bentheimer’     

A.D.M. Biermann  E.C.G. Pimentel  M. Tietze  T. Pinent  S. König 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2014,131(1):36-45

A pedigree including 1538 individuals of the endangered pig breed ‘Bunte Bentheimer’ and 3008 records of the fertility traits ‘number of piglets born alive’ (NBA) and ‘number of piglets weaned’ (NW) were used to i) characterize the population structure, ii) to estimate genetic (co)variance components and estimated breeding values (EBVs) and iii) to use EBVs for the application of the concept of optimal genetic contributions. The average coefficient of inbreeding increased from F = 0.103 to F = 0.121 within the two recent cohorts. Average rate of inbreeding amounted to 1.66%, which resulted in an effective population size of N_e = 30 animals in the recent cohort. Average generation interval was 3.07 years considering the whole pedigree, and in total, only 612 sows and boars generated offspring. Estimated heritabilities for both traits NBA and NW were 0.12, and the estimated genetic correlation between both traits was 0.96. The variance component due to the service sire was higher than in commercial pig breeds, presumably due to the widespread use of natural service boars. The EBVs for NBA from 333 selection candidates (63 boars and 270 sows) were used to determine optimal genetic contributions. Based on selected animals and their optimal genetic contributions, specific mating designs were evaluated to minimize inbreeding in the next generation. Best results were achieved when using a simulated annealing algorithm and allowing artificial insemination.  相似文献   

Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86)   总被引：6，自引：0，他引：6  

Patarroyo JH  Portela RW  De Castro RO  Pimentel JC  Guzman F  Patarroyo ME  Vargas MI  Prates AA  Mendes MA 《Veterinary immunology and immunopathology》2002,88(3-4):163-172

Three synthetic peptides (SBm4912, SBm7462 and SBm19733), derived from the Bm86 glycoprotein from Boophilus microplus gut, were constructed and used to immunize cattle from a tick-free area. The immunized animals received three subcutaneous doses of the peptides, with saponin as adjuvant, at 30-day intervals. The immune response was evaluated by IgG elicited against the peptides by the detection of anti-Bm86 specific antibodies in situ and by Western blotting analysis. After tick challenge, reduction in the number, weight and oviposition capacity of engorged females was observed in the tick population that had fed on immunized animals. The results pointed a high efficacy (81.05%) for the SBm7462 synthetic peptide in relation to the others (p<0.01), demonstrating the efficiency of the immune response elicited by synthetic peptides to control the cattle tick B. microplus.  相似文献   

First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from different regions of Brazil     

de Albuquerque  Pedro Paulo Feitosa  de Melo  Renata Pimentel Bandeira  de Farias Brito  Marilene  Bovino  Fernanda  de Souza  Mariana Assunção  Lima  Anna Monteiro Correia  de Oliveira  Emerson Antônio Araújo  de Moraes Pereira  Helder  Mota  Rinaldo Aparecido 《Tropical animal health and production》2018,50(8):1929-1935

Tropical Animal Health and Production - Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The...  相似文献   

Spatial regression analysis of Ceratitis capitata Wiedemann (Diptera: Tephritidae) on Terceira Island,Azores     

R. Pimentel  D.J.H. Lopes  A.M.M. Mexia  J.D. Mumford 《国际虫害防治杂志》2014,60(3):217-223

The fruit fly Ceratitis capitata Wiedemann is one of the most important threats to the trade of fresh fruits in the world due to its ability to survive in a wide range of hosts and climatic conditions. The main goal of this study is to evaluate if there is any significant relationship between the abundance of wild adults of C. capitata and the spatial characteristics of the location. The ordinary least squares estimation model calculated for each gender cannot provide a satisfactory general explanation for abundance of both genders of wild adults, yet it might generate some hypotheses about wild adult females of C. capitata fruit-seeking behaviour. Results from geographically weighted regression analysis can provide a satisfactory general explanation for abundance of both genders of wild adults. Both methods suggest that males are more dispersed than females, and because of that they might play an important role in scouting the surroundings for additional fruit hosts. The presence of some host-plants, even in places offering less protection (like pasture areas), provides an ecological corridor that supports the spread of wild adults of C. capitata.  相似文献