d-Lactic Acidosis in Neonatal Ruminants     

Ingrid Lorenz  Arcangelo Gentile 《Veterinary Clinics of North America: Food Animal Practice》2014

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Collection,ex situ conservation and characterization of mangaba (Hancornia speciose Gomes) germplasm in coastal lowland of Northeastern Brazil     

da Silva  Ana Veruska Cruz  Oliveira  Jéssica Monalisa S. P.  Cardoso  Milena Nascimento  Nascimento  Ana Letícia Sirqueira  Soares  Tássia Fernanda S. N.  da Silva Júnior  Josué Francisco  da Silva Lédo  Ana  Muniz  Evandro Neves 《Genetic Resources and Crop Evolution》2021,68(6):2441-2453

Genetic Resources and Crop Evolution - This study aimed to evaluate the Mangaba Genebank of Embrapa Tabuleiros Costeiros, Brazil, since its implementation in 2006 and characterize the accessions in...  相似文献   

Glomerular lesions in dogs infected with Leishmania organisms     

Zatelli A  Borgarelli M  Santilli R  Bonfanti U  Nigrisoli E  Zanatta R  Tarducci A  Guarraci A 《American journal of veterinary research》2003,64(5):558-561

OBJECTIVE: To histologically identify glomerular lesions in dogs infected with Leishmania organisms. ANIMALS: 41 dogs (17 sexually intact males and 14 sexually intact and 10 ovariohysterectomized females) that had positive results when tested for leishmaniosis as determined by use of serologic evaluation (indirect fluorescent antibody test, titers of 1:80 to 1:640) and direct microscopic identification of the protozoal organisms. PROCEDURE: Urine samples were collected by use of cystocentesis and examined by qualitative SDS-agarose gel electrophoresis (AGE). All dogs had non-selective (glomerular) or mixed (glomerular and tubular) proteinemia. Specimens were obtained from each dog during ultrasound-assisted renal biopsy and used for histologic examination. Each specimen was stained with H&E, periodic acid-Schiff, Goldner's trichrome, methenamine silver, and Congo Red stains. Specimens were adequate for evaluation when they contained at least 5 glomeruli/section, except for specimens stained with Congo Red in which 1 glomerulus/section was adequate. RESULTS: Examination of renal biopsy specimens revealed various glomerular lesions in all dogs and interstitial or tubular (or both) lesions in 23 of 41 (55%) dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Glomerular lesions that develop in dogs during infection with Leishmania organisms can be classified histologically as mesangial glomerulonephritis, membranous glomerulonephritis, membranoproliferative glomerulonephritis, and focal segmental glomerulonephritis. Tubulointerstitial histopathologic conditions were not observed as the primary lesion, despite being evident in 23 of 41 (55%) dogs. Use of SDS-AGE for qualitative evaluation of proteinuria and successive collection of specimens during renal biopsies following diagnosis of nonselective glomerular proteinuria provides the possibility for early identification of renal lesions.  相似文献   

Adrenocortical suppression in the dog after a single dose of methylprednisolone acetate     

R J Kemppainen  M D Lorenz  F N Thompson 《American journal of veterinary research》1981,42(5):822-824

Adrenal function was assessed in dogs after intramuscular administration of a single dose of methylprednisolone acetate (MPA). Twelve dogs were test challenged with adrenocorticotropic hormone (ACTH) and then assigned randomly to 1 of 3 groups and given MPA. Individual groups were test challenged with ACTH 2, 3, or 4 weeks later. All dogs were rechallenged 5 weeks after MPA administration. Plasma cortisol concentration was determined by radioimmunoassay. Basal plasma cortisol (time 0) was depressed on weeks 2 and 3, but not on weeks 4 and 5. Adrenal response to ACTH (increment of cortisol change) was suppressed on weeks 2, 4, and 5, but not on week 3. It was concluded that a single dose of MPA is capable of altering adrenal cortical function in dogs for at least 5 weeks.  相似文献   

Enzyme-linked immunosorbent assay for detection of bluetongue virus antibodies   总被引：4，自引：0，他引：4  

O J Hübschle  R J Lorenz  H D Matheka 《American journal of veterinary research》1981,42(1):61-65

The immune response to bluetongue virus in sheep and cattle was studied by applying a newly developed indirect enzyme-linked immunosorbent assay (ELISA). Purified virus obtained by sucrose gradient centrifugation was used at a concentration of 0.01 optical density units (formula: see text) to coat individual wells (200 microliter) of a microtitration plate. Dilution of antigen was performed in 0.05 M carbonate buffer, pH 9.6, and adsorption lasted for at least 16 hours at 4 C. Coated plates retained their activity for 10 weeks when stored at 4 C. Sera recovered from experimentally infected sheep and cattle were tested together with known negative sera. A good correlation between results was obtained with the modified complement-fixation test and the ELISA; however, the ELISA proved to be more sensitive. The group specificity of the ELISA was proven by testing various type-specific sheep and cattle immune sera. The ELISA has potential for the detection of group-specific antibodies to bluetongue virus infection.  相似文献   

Investigations on the influence of serum D-lactate levels on clinical signs in calves with metabolic acidosis     

Lorenz I 《Veterinary journal (London, England : 1997)》2004,168(3):323-327

Correlations between the degree of acidosis and clinical signs (changes in posture, behaviour, intensity of suckling reflex) in neonatal diarrhoeic calves have been described in various studies. However, base excess values varied widely in calves exhibiting similar clinical symptoms. The objective of this study was to elucidate whether the clinical picture of acidotic calves with neonatal diarrhoea is influenced more by D-lactate concentration than by degree of acidosis. Eighty calves up to three weeks old that were admitted to the II Medical Animal Clinic with acute diarrhoea and base excess values between -10 and -25 mmol/L were included in the prospective study. Posture, behaviour, suckling and palpebral reflexes, and position of the eyeballs were scored during the initial examination. Base excess and serum D-lactate and urea concentrations were determined in venous blood. In order to quantify the influences of base excess and d-lactate on the clinical parameters, groups of different clinical categories were compared. The results show that variations in behaviour, and in posture can be better explained by elevations of serum D-lactate concentrations than by decreases in base excess. Disturbances of the palpebral reflex appear to be almost completely caused by high levels of D-lactate.  相似文献   

Honigbienen zur Bestäubung von verfrühten Süßkirschen im geschützten Anbau     

Andreé Hamm  Jürgen Lorenz  Dete Papendieck  Olesya Dekova  Michael Blanke 《Erwerbs-Obstbau》2007,49(3):85-92

The objective of the present work was to study ways of additional foraging of honey bees in a sustainable production of forced sweet cherries in a polytunnel in order to reduce any loss of bees and to substitute the annual re-introduction of new bumble bees (Bombus terrestris L.) which are lost after use. Three honey bee (Apis mellifera L.) hives were employed during the three weeks flowering in April 2006 in a closed, unheated polytunnel to pollinate sweet cherry cvs ‘Burlat’, ‘Earlise’, ‘Samba’, ‘Souvenir des Charmes’ and an unnamed clone on dwarfing GiSelA 5 rootstock at Klein Altendorf research station near Bonn, Germany. The central position of hive A allowed the honey bees to collect pollen and nectar exclusively from the flowering sweet cherries in the tunnel. Hive B also inside the tunnel had apertures inside and outside the tunnel, while hive C outside the tunnel adjacent to its wall, had inside and outside apertures; honey bees from the latter two hives were allowed to forage both inside the tunnel or outside. Differences in air temperatures between the inside and outside the tunnel were no more than 5?°C due to effective ventilation. A portable gas heater for automated frost protection maintained the night temperature in the polytunnel in April above 0?°C. Measurements of the flight activity showed a close correlation with the ambient temperature at the respective hive location. The two bee hives A and B within the tunnel showed larger flight activities of an averaged 540 bees per hour compared with 180 bees per hour outside due to low spring temperatures. Honey bee population A in the centre of the tunnel declined after three weeks pollination to ca. 9,200 individuals and produced only 46?kg honey due to shortage in food reserves and intense flight activity. The outdoor bee population C with ca. 15,000 individuals yielded also only 49?kg honey due to lack of flight activity during the cold April 2006 compared with 68?kg honey from population B with ca. 16,000 individuals but continuous flight activity within the tunnel and additional foraging outside. Control bee hives D and E with ca. 22,000 individuals each, which had remained at the institute at Bonn, produced 82–93?kg honey during the same time. Cherry forcing resulted in a 12–18 days earlier flowering in April 2006 relative to the 12–16 days earlier harvest in June 2006. Large fluctuations in yield of 0.3–15.4?kg/tree were due to commensurate S-alleles causing intersterility, insufficient fertilisation and location of the cherry cultivars relative to each other and the bee hive.  相似文献   

Methodische Untersuchungen zur Trennung und Bestimmung von organischen Säuren in Pflanzenmaterial     

F. Scheffer  R. Kickuth  H. Lorenz 《Plant foods for human nutrition (Dordrecht, Netherlands)》1965,12(4):342-348

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Myopathy associated with hyperadrenocorticism in the dog   总被引：1，自引：0，他引：1  

C E Greene  M D Lorenz  J F Munnell  K W Prasse  N A White  J M Bowen 《Journal of the American Veterinary Medical Association》1979,174(12):1310-1315

Naturally occurring or iatrogenic hyperadrenocorticism was associated with myopathy in six dogs. One dog had muscle weakness and muscle atrophy but normal electromyographic findings. Five dogs had muscle stiffness, proximal appendicular muscle enlargement, and myotonic discharges on electromyography. Histologic, electron microscopic, and histochemical findings in the musculature of dogs that were examined were characteristic of noninflammatory degenerative myopathy. Clinical signs of the myopathy improved to varying degrees in five dogs that were treated for the hyperadrenocorticism.  相似文献   

The reused progesterone device has the same effect on short or long estrus synchronization protocols in tropical sheep     

Biehl  Marcos Vinicius  de Ferraz Junior  Marcos Vinicius Castro  Barroso  José Paulo Roman  Susin  Ivanete  Ferreira  Evandro Maia  Polizel  Daniel Montanher  Pires  Alexandre Vaz 《Tropical animal health and production》2019,51(6):1545-1549

This study aimed to evaluate the effect of progesterone (P4) device reutilization in long and short protocols for transcervical timed artificial insemination (TAI) in Santa Inês ewes. A total of 275 multiparous lactating ewes were blocked according to body weight (BW, 49.1?±?7.3 means ± SE), body condition score (BCS, 2.9?±?0.4; scale of 1–5), and days postpartum (50?±?8.2 days), and allocated to one of the treatments. The treatments were arranged in a factorial design, in which the factor 1 was the P4 device type (new or a device of 0.3 g of P4 previously used by 11 days), and the factor 2 was the short or long TAI protocol (P4 device remained by 7 or 11 days, respectively). At device removal, all ewes received 300 IU eCG and 6.70 mg of Dinoprost tromethamine. After TAI protocol, ewes remained with ram by 21 days. There was no interaction between factors in any variables. Ewes that received a new P4 device delayed (P?=?0.05) to show estrus compared with ewes receiving a previously used P4 device, but it did not affect pregnancy rate. The long protocol tended to increase pregnancy rate compared with short protocol (33% vs. 24%, respectively; P?=?0.07). However, the pregnancy rate at the end of reproductive period was similar in both groups (about 84%). Thus, the use of long protocols tended to improve reproductive performance, and the reused P4 device did not affect pregnancy rate.

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