Effects of Biochar and Marble mud on Mine Waste Properties to Reclaim Tailing Ponds     

Maria Angeles Muoz  Jose German Guzman  Raul Zornoza  Fabian Moreno  Angel Faz  Rattan Lal 《Land Degradation \u0026amp; Development》2016,27(4):1227-1235

The effects of biochar addition in improving soil physical properties are not clearly understood in mining tailings. The objective of this study was to determine the effects of three different types of biochars, in addition to marble mud (MM) and their mixtures, on the structural stability and water retention of mine wastes in Cartagena, Spain. Biochars were produced at 500 °C from pig manure (PM), cotton (Gossipium hirsutum L.) crop residues (CR) and municipal solid waste (MSW). Biochars were added to the mine waste (MW) along with MM and a control (no amendments added). These mixtures were incubated in cores for 90 days (25 °C). PM and CR mixed with MM decreased soil bulk density (from 0 · 98 g cm^‐3 to 0 · 89 and 0 · 84 g cm⁻³, respectively). Amendments had no significant effect on total porosity whereas they increased gas diffusion by 100%, except for MSW. MM improved the plant available water from 0 · 59 to 2 · 56 cm as its combination with biochars, extremely relevant in water scarce climates. The micropores were likely replaced by mesopores when application of PM, CR, MM, and biochars + MM and they improved water retention. Total carbon (TC) and total nitrogen (TN) increased by using biochars and MM and no significant effects were assessed on aggregates. In general, MM mixed with PM and CR derived biochar improved the structural stability and exhibited a strong impact in reclaiming physical quality on mine tailings. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

The application of polyamines by pressure or immersion as a tool to maintain functional properties in stored pomegranate arils     

Mirdehghan SH  Rahemi M  Serrano M  Guillén F  Martínez-Romero D  Valero D 《Journal of agricultural and food chemistry》2007,55(3):755-760

Pomegranate fruits were treated with putrescine (Put) or spermidine (Spd) at 1 mM either by pressure infiltration or by immersion and then were stored at 2 degrees C for 60 days. Samples were taken biweekly and were further stored 3 days at 20 degrees C for shelf life study. The treatments were effective on maintaining the concentration of ascorbic acid, total phenolic compounds, and total anthocyanins in arils at higher levels than in control samples. In addition, the two ways of polyamine application increased the levels of total antioxidant activity (TAA) during storage, especially when polyamines were applied by pressure infiltration. Moreover, Spd showed the best results on increasing TAA through maintenance of total phenolic compounds.  相似文献   

Diagnostics of Dairy and Beef Cattle Diarrhea   总被引：1，自引：0，他引：1  

Patricia Carey Blanchard 《Veterinary Clinics of North America: Food Animal Practice》2012

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Sperm quality following 4 days of cooled storage: impact on fertility     

A.M. KiserA.K. Sudderth  C.C. LoveS.P. Brinsko  D.D. VarnerT.L. Blanchard 《Journal of Equine Veterinary Science》2012

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An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome   总被引：14，自引：0，他引：14  

Blanchard P  Mahé D  Cariolet R  Truong C  Le Dimna M  Arnauld C  Rose N  Eveno E  Albina E  Madec F  Jestin A 《Veterinary microbiology》2003,94(3):183-194

Porcine circovirus type 2 (PCV2) plays a crucial role in the pathogenesis of post-weaning multisystemic wasting syndrome (PMWS) in swine. As PCV2 displays significant homology with PCV1 (a non-pathogenic virus) at the nucleotide and amino-acid level, a discriminative antigen is needed for specific serological diagnosis. The ORF2-encoded capsid protein from PCV2 was used to develop an indirect enzyme-linked immunosorbent assay (ELISA). GST-fused capsid protein from PCV2 and GST alone (both expressed in recombinant baculovirus-infected cells) were used as antigens for serodiagnosis. The specificity of the ELISA for detection of PCV2 antibodies was demonstrated in sera from pigs experimentally infected with PCV1, PCV2 and other swine viruses. The semi-quantitative nature of the test was evaluated versus an immunoperoxidase monolayer assay (IPMA). The ELISA was performed on 322 sera from pigs in eight Brittany herds and compared with IPMA. The sensitivity (98.2%) and specificity (94.5%) of this test were considered suitable for individual serological detection. High PCV2 seroprevalence was found in sows and pigs at the end of the growth phase (18-19 weeks) in all eight herds. The seroprevalence in piglets (11-17 weeks) was statistically correlated with clinical symptoms of PMWS (93% in affected versus 54%, in non-affected farms). A cohort study performed in PMWS-free farms showed that 57% of piglets exhibited active seroconversion after 13 weeks, indicating that PCV2 infection occurred earlier in PMWS-affected piglets.  相似文献   

Semen Processing for the Subfertile Stallion     

Dickson D. Varner  Charles C. Love  Steven P. Brinsko  Terry L. Blanchard  David L. Hartman  Shalyn B. Bliss  Brian S. Carroll  Mark C. Eslick 《Journal of Equine Veterinary Science》2008

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The treatment of pannus in dogs by use of a corneal-scleral graft     

E L Kuhns  W F Keller  G L Blanchard 《Journal of the American Veterinary Medical Association》1973,162(11):950-952

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Metastrongylus im Liquorraum eines Katzengehirnes     

Gerda Fabian 《Zoonoses and public health》1975,22(6):524-526

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The use of hydrophilic contact lenses in corneal diseases of the dog and cat: A preliminary report     

G. M. Schmidt  G. L. Blanchard  W. F. Keller 《The Journal of small animal practice》1977,18(12):773-777

ABSTRACT
Two types of hydrophilic contact lenses were tested in dogs and cats as part of the therapeutic régime of recurrent corneal erosions, superficial corneal ulcers and keratoconjunctivitis sicca. It was found that the lenses are well tolerated by these species when properly fitted to the cornea. If the lenses were not properly fitted, they were lost before their therapeutic effectiveness could be evaluated. The use of hydrophilic contact lenses appeared to be beneficial in the treatment of superificial corneal ulcers and recurrent corneal erosions in the dog and cat, but ineffective in the treatment of keratoconjunctivitis sicca.  相似文献   

The complex folding network of single calmodulin molecules     

Stigler J  Ziegler F  Gieseke A  Gebhardt JC  Rief M 《Science (New York, N.Y.)》2011,334(6055):512-516

Direct observation of the detailed conformational fluctuations of a single protein molecule en route to its folded state has so far been realized only in silico. We have used single-molecule force spectroscopy to study the folding transitions of single calmodulin molecules. High-resolution optical tweezers assays in combination with hidden Markov analysis reveal a complex network of on- and off-pathway intermediates. Cooperative and anticooperative interactions across domain boundaries can be observed directly. The folding network involves four intermediates. Two off-pathway intermediates exhibit non-native interdomain interactions and compete with the ultrafast productive folding pathway.  相似文献