全文获取类型
收费全文 | 875篇 |
免费 | 90篇 |
专业分类
林业 | 79篇 |
农学 | 38篇 |
基础科学 | 1篇 |
128篇 | |
综合类 | 24篇 |
农作物 | 54篇 |
水产渔业 | 127篇 |
畜牧兽医 | 419篇 |
园艺 | 19篇 |
植物保护 | 76篇 |
出版年
2024年 | 1篇 |
2023年 | 12篇 |
2022年 | 35篇 |
2021年 | 54篇 |
2020年 | 53篇 |
2019年 | 62篇 |
2018年 | 45篇 |
2017年 | 49篇 |
2016年 | 58篇 |
2015年 | 34篇 |
2014年 | 49篇 |
2013年 | 50篇 |
2012年 | 66篇 |
2011年 | 70篇 |
2010年 | 35篇 |
2009年 | 30篇 |
2008年 | 42篇 |
2007年 | 43篇 |
2006年 | 22篇 |
2005年 | 24篇 |
2004年 | 23篇 |
2003年 | 13篇 |
2002年 | 20篇 |
2001年 | 8篇 |
2000年 | 12篇 |
1999年 | 11篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1973年 | 1篇 |
1969年 | 2篇 |
1967年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有965条查询结果,搜索用时 15 毫秒
71.
A novel vasodilatory peptide from the salivary glands of the sand fly Lutzomyia longipalpis 总被引:4,自引:0,他引:4
Salivary gland lysates of the sand fly Lutzomyia longipalpis contain a potent vasodilator that aids the fly to feed on the blood of its vertebrate hosts. Chromatographic analysis, antibody reactivity, and data obtained from bioassays of the salivary erythema-inducing factor indicate striking similarity with human calcitonin gene-related peptide. The erythema-inducing factor is, however, at least one order of magnitude more potent than calcitonin gene-related peptide. 相似文献
72.
Almeida MP de Melo F Hor-Meyll M Salles A Walborn SP Ribeiro PH Davidovich L 《Science (New York, N.Y.)》2007,316(5824):579-582
We demonstrate the difference between local, single-particle dynamics and global dynamics of entangled quantum systems coupled to independent environments. Using an all-optical experimental setup, we showed that, even when the environment-induced decay of each system is asymptotic, quantum entanglement may suddenly disappear. This "sudden death" constitutes yet another distinct and counterintuitive trait of entanglement. 相似文献
73.
Mulugu S Bai W Fridy PC Bastidas RJ Otto JC Dollins DE Haystead TA Ribeiro AA York JD 《Science (New York, N.Y.)》2007,316(5821):106-109
Inositol pyrophosphates are a diverse group of high-energy signaling molecules whose cellular roles remain an active area of study. We report a previously uncharacterized class of inositol pyrophosphate synthase and find it is identical to yeast Vip1 and Asp1 proteins, regulators of actin-related protein-2/3 (ARP 2/3) complexes. Vip1 and Asp1 acted as enzymes that encode inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase activities. Alterations in kinase activity led to defects in cell growth, morphology, and interactions with ARP complex members. The functionality of Asp1 and Vip1 may provide cells with increased signaling capacity through metabolism of IP6. 相似文献
74.
Machado S. Bynum E. D. Archer T. L. Bordovsky J. Rosenow D. T. Peterson C. Bronson K. Nesmith D. M. Lascano R. J. Wilson L. T. Segarra E. 《Precision Agriculture》2002,3(4):389-406
This study was conducted to determine relationships between biotic and abiotic factors and to generate information needed to improve the management of site-specific farming (SSF). The effects of water (80% evapotranspiration (ET) and 50% ET), hybrid (drought-tolerant and -susceptible), elevation, soil texture, soil NO3--N, soil pH, and greenbugs (Schizaphis graminum) (Gb) on sorghum grain yield were investigated at Halfway, TX on geo-referenced locations on a 30-m grid in 1997, 1998, and 1999. Grain yields were influenced by interrelationships among many factors. Grain yields were consistently high under 80% ET treatment and in the upper slopes where the clay and silt fractions of the soil were high. Soil NO3--N, rainfall, hybrid, and Gb effects on grain yields were seasonally unstable. Soil NO3--N increased grain yield when water was abundant and depressed grain yields when water was limiting. Plant density effects on grain yield were confounded with hybrid responses to drought and Gb infestation. Managing seasonally unstable factors is a major challenge for farmers and better ways to monitor crop growth and diagnose causes of poor plant growth are needed. To improve the management of SSF, effects of the relationships between biotic and abiotic factors on crop yield must be integrated and evaluated as a system. Based on our study, information on seasonally stable factors like elevation and soil texture is useful in identifying management zones for water and fertilizer application. Water and fertilizers management should be complemented by in-season management of seasonally unstable factors like soil NO3--N, rainfall, hybrid, and Gb effects on grain yield. 相似文献
75.
André MR Adania CH Teixeira RH Allegretti SM Machado RZ 《Journal of zoo and wildlife medicine》2011,42(1):139-143
Large and small piroplasms have been observed in the blood smears of various wild carnivores, but few studies utilizing molecular characterization have been done. The goal of this present study was to investigate the presence of Babesia sp. by molecular and serologic techniques in exotic and neotropical carnivores maintained in captivity at Brazilian zoos. Blood and sera samples were collected from 146 Brazilian wild felids, 21 exotic felids, 1 genet (Genetta tigrina), 3 European wolves (Canis lupus), and 94 Brazilian wild canids in Brazilian zoos in the S?o Paulo and Mato Grosso states and in the Federal District. A total of 53 wild felids (31.74%) and 10 wild canids (10.31%) were seropositive for Babesia canis by Indirect Fluorescent Antibody Test (IFAT). Antibodies were detected in ocelots, little-spotted cats, margays, pampas cats, jaguars, pumas, jaguarundis, crab-eating foxes, and bush dogs. Babesia sp. DNA, with high similarity to B. leo, was detected in one pampas cat and one genet. 相似文献
76.
S Herr J V Lawrence O L Brett L M Ribeiro 《The Onderstepoort journal of veterinary research》1991,58(2):111-114
Brucella abortus and B. melitensis antigens were used in parallel on the National Standard Brucella abortus antiserum and on field sera coming from cattle where practically exclusively B. abortus biotypes 1 and 2 have been isolated over the last 11 years. With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. Most were 1 dilution (twofold) lower. Although a similar trend was seen with the field sera, there were 7/346 sera which had twofold or higher titres to B. melitensis antigen. Although this may be due to the vagaries of the test it also warrants closer investigation of the animals concerned to see whether M-antigen predominant Brucella biotypes are possibly present. The use of the dual antigens could identify herds which are infected only with A-antigen predominant brucellae but would not be reliable for classifying individual animals. 相似文献
77.
Dvorak CM Hyland KA Machado JG Zhang Y Fahrenkrug SC Murtaugh MP 《Veterinary immunology and immunopathology》2005,105(3-4):301-315
Peyer's patches of the intestinal mucosa are essential for host defense and immune regulation in the enteric system. To better understand molecular mechanisms of Peyer's patch function, we have screened for differentially expressed genes specific to Peyer's patch. cDNA libraries were created from normal Peyer's patch, immune stimulated Peyer's patch, and pooled cDNA subtracted with fibroblast RNA. From the subtracted library, 3687 expressed sequence tags (ESTs), representing 2414 unique nucleotide sequences, were isolated, identified by BLAST searches against public databases, and spotted onto a microarray for gene expression profiling. Approximately 30% of these ESTs BLAST to genes of unknown function and 20% have no known homology in the public databases (novel genes). Of the novel genes, 70% are expressed in normal immune tissues by microarray analysis, suggesting that at least 371 of the unidentified EST sequences from the subtracted library are novel porcine genes and can now be further characterized to determine their function in the porcine Peyer's patch. We surmise that the products of these genes participate in biochemical and cellular functions related to the unique immunological and gastroenterological functions of the small intestine. The BLAST and gene ontology information for each of the subtracted library EST sequences, the normal and immune stimulated libraries, and the microarray are all valuable resources that will facilitate further examination of the biological function of porcine Peyer's patch tissue. 相似文献
78.
Souza AC Machado FS Celes MR Faria G Rocha LB Silva JS Rossi MA 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(5):230-237
The present study was designed to identify, submicroscopically, the primary organelle or target structure for monensin in cultured murine fibroblasts L929. In addition, the effect of the drug on cell size and surface membranes of the cells were analysed; cellular proliferation, collagen secretion, and necrosis and apoptosis were re-evaluated. At the lowest concentration of monensin the foremost ultrastructural alteration occurred in the mitochondria, characterized by increased matrix density with disorganized and less distinct crystae. Incubation with monensin at higher concentrations resulted in severe mitochondrial damage and marked dilatation of the Golgi apparatus and rough endoplasmic reticulum cisternae. Fibroblasts exposed to higher concentrations of monensin were enlarged with decreased number of filopodia and hollows in the surface membrane. Moreover, monensin inhibited the cell proliferation, increased immunohistochemical positiveness for collagen type I in a dose-dependent manner, and, at high concentrations, caused cell necrosis whereas apoptosis was not induced. Taken together, these results show that monensin induces early mitochondrial damage, possibly causing an energy deficit that led to inhibition of fibroblasts proliferation and accumulation of collagen causing dilatation of Golgi apparatus and rough endoplasmic reticulum. Moreover, the mitochondrial damage would also explain the monensin-induced necrosis. 相似文献
79.
Growth and photochemical response of triazine-susceptible and -resistant rutabaga ‘Laurentian’ genotypes to cyanazine (chloro-s-triazine) and metribuzin (methylthio-as-triazine) were evaluated. The young seedlings of the susceptible rutabaga were killed when cyanazine or metribuzin were applied either pre- or post-emergence. The triazine-resistant rutabaga, however, displayed a differential response to these herbicides. Metribuzin applied pre-emergence killed the seedlings at 0.4 kg ha?1, and at 0.2 kg ha?1 the growth was severely affected. Cyanazine even at higher rates applied pre- or post-emergence failed to inhibit growth in these plants.Chlorophyll fluorescence in leaf sections at the cotyledonary, 2-leaf and 4-leaf stages in the susceptible plants increased by 130 and 172% in response to 10?5 M cyanazine and metribuzin, respectively, suggesting that Photosystem II reactions in these plants were severely impaired. In resistant plants, there was little or no leaf chlorophyll fluorescence (LCF) increase at this herbicide concentration. However, at 10?4 M cyanazine and metribuzin, the LCF in resistant plants increased significantly and the increase was greater in response to metribuzin (86%) than to cyanazine (51%). This indicates that with these triazine-resistant genotypes, metribuzin is relatively more potent and at high rates is capable of interfering with photosynthesis. 相似文献
80.
Clinical signs, humoral and cellular immune responses, and microscopic and gross tissue alterations resulting from acute experimental Ehrlichia canis infection in dogs were studied. Four dogs were inoculated with E. canis and four were used as uninfected controls. After a 10-14-day incubation period, infected dogs developed pyrexia up to 41 degrees C for 6-8 days. Antibody titers to E. canis antigen were demonstrable in all inoculated dogs at 30 days post-infection. Necropsy of infected animals revealed pale mucous membranes, generalized lymphadenopathy, splenomegaly, edema and ascites. Microcopically, the main lesions were: lymphoreticular hyperplasia in cortical areas of lymph nodes and spleenic white pulp, periportal accumulation of mononuclear cells and centrolobular fatty degeneration of the liver. Kidneys presented with glomerulonephritis characterized by interstitial mononuclear infiltration. Immunophenotyping of lymphocytes from lymph nodes and spleen sections displayed alterations in IgG, IgM, CD3+ and CD8+ cells population in infected dogs. 相似文献