Effect of biologically active substances present in water extracts of white mustard and coriander on antioxidant status and lipid peroxidation of mouse C2C12 skeletal muscle cells          下载免费PDF全文

K. Zielniok  K. Szkoda  M. Gajewska  J. Wilczak 《Journal of animal physiology and animal nutrition》2016,100(5):988-1002

Coriander and white mustard, an annual plants originated in the Mediterranean region, have been cultivated and used as spices for a long time. Recent studies have shown that they may constitute a potential source of phenolic compounds. The aim of this study was to evaluate the content of polyphenols in coriander and white mustard water extracts and to investigate their antioxidant activity in C2C12 mouse skeletal muscle cells, which serve as a good model of cells with intensive metabolism. HPLC analysis showed that polyphenols were able to permeate from the water extracts of studied plants into the undifferentiated myoblasts as well as myocytes undergoing differentiation, increasing the concentration of reduced glutathione and upregulating glutathione reductase and peroxidase activity. White mustard and coriander extracts also decreased the levels of oxysterols and sum of tiobarbituric acid reactive substances (TBARS) in both: myoblasts and differentiating myocytes, demonstrating protective effect on cell membranes. The obtained results indicate that polyphenols synthesized by both herbs may have beneficial effects on muscle tissue.  相似文献   

Content and distribution of different phosphorus forms in broiler chickens          下载免费PDF全文

X.‐K. Li  J.‐Z. Wang  C.‐H. Zhang  X. Li  C.‐H. Tang 《Journal of animal physiology and animal nutrition》2016,100(2):271-278

To understand the background value of phosphorus in chickens, the quantitative distribution of different phosphorus forms, including total phosphorus (TP), free phosphate (FP) and phospholipid (PL), in viscera, blood and bones of broiler chickens was investigated. Results showed that phosphorus contents exhibited significant differences in different parts of chickens. TP content of breast and thigh meat was over 5.0 g/kg, in which most of the phosphorus was in the form of water‐soluble phosphates. TP content in viscera was higher than that in meat, and spleen was observed to contain the highest amount of phosphorus (10.0 g/kg). In all tested organs, FP and PL contents in liver were the highest, ranging between 1207–1989 and 81–369 mg/kg respectively. TP content in chicken bone was in the range of 52 716–136 643 mg/kg, and FP content in the bone was relatively lower than that in chicken meat.  相似文献   

Estimation of the standardized ileal digestible lysine requirement for primiparous pregnant sows          下载免费PDF全文

M. Shi  C. X. Shi  Y. K. Li  D. F. Li  F. L. Wang 《Journal of animal physiology and animal nutrition》2016,100(2):287-293

This experiment was conducted to determine the optimal standardized ileal digestible lysine (SID Lys) level in diets fed to primiparous sows during gestation. A total of 150 (Landrace × Large White) crossbred gilts (weighing 149.9 ± 3.1 kg) were fed gestation diets (12.55 MJ of ME/kg) containing SID Lys levels of 0.43, 0.52, 0.60, 0.70 or 0.80% respectively. Gilts were fed 2.0 kg/day from day 1 to 80 and 3.0 kg/day from day 80 to 110 of gestation respectively. Gilts were allocated to treatments based on their body weight on the day of breeding. Weight gain from day 80 to 110 increased with increasing dietary SID Lys levels (p = 0.044). Fitted broken‐line (p = 0.031) and quadratic plot (p = 0.047) analysis of body weight gain indicated that the optimal SID Lys level for primiparous sows was 0.70 and 0.69% respectively. During gestation, neither backfat thickness nor loin eye area was affected by dietary SID Lys level. Increasing dietary Lys had no effect on the litter size at birth or pigs born alive per litter. Litter weight at birth was not affected by dietary SID Lys level. The litter weight variation at birth quadratically decreased with increasing dietary SID Lys (p = 0.021) and was minimized at 0.70% dietary SID Lys. Gilts fed the 0.70% SID Lys diet had the highest dry matter (p = 0.031) and protein (p = 0.044) content in colostrum. On day 110 of gestation, gilts fed the 0.70% SID Lys diet tended to have the highest serum prolactin (p = 0.085) and serum insulin (p = 0.074) levels. The data demonstrate that the optimal dietary SID Lys was 0.70% for pregnant gilts, which is similar to the recommendation of 0.69% that was estimated by the NRC (2012).  相似文献   

Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin‐like growth factor‐1 in pre‐pubertal Holstein heifers          下载免费PDF全文

C. Kawashima  M. Munakata  M. Matsui  A. Miyamoto  K. Kida  T. Shimizu 《Journal of animal physiology and animal nutrition》2016,100(6):1037-1040

Insulin‐like growth factor‐1 (IGF‐1) is one of the important factors for growth, milk production and reproductive functions and mainly released from the liver in response to growth hormone (GH) via GH receptor (GHR) in cattle. Recently, some single nucleotide polymorphisms (SNPs) were identified in the bovine GHR gene. Some GHR‐SNPs were shown to be related to plasma IGF‐1 concentration in cattle. Hence, the capacity to IGF‐1 production in the liver might be affected by GHR‐SNP and associated with performance in the future. This study examined whether GHR‐SNP is associated with IGF‐1 production in the liver of pre‐pubertal heifers. In 71 Holstein calves, blood samples for genomic DNA extraction were obtained immediately after birth. To genotype the GHR‐SNPs in the promoter region, polymerase chain reaction (PCR) products were digested with restriction enzyme NsiI (cutting sites: AA, AG and GG). All heifers at 4 months of age were intramuscularly injected with 0.4 mg oestradiol benzoate. Blood samples were obtained from the jugular vein just before (0 h) and 24 h after injection. The number of AA, AG and GG at the NsiI site was 0, 17 and 54 respectively. In AG and GG, plasma GH concentrations were higher pre‐injection than 24 h post‐injection (p < 0.01). Moreover, plasma GH concentrations in AG post‐injection were higher than in GG (p < 0.05). In contrast, the GG genotype exhibited higher plasma IGF‐1 concentrations in pre‐injection than post‐injection (p < 0.01), although oestradiol did not change IGF‐1 concentration in the AG genotype. We conclude that the GG polymorphism in the promoter region of GHR is associated with a higher potential capacity of IGF‐1 production in the liver of cattle.  相似文献   

Pregnancy‐Induced ISG‐15 and MX‐1 Gene Expression is Detected in the Liver of Holstein–Friesian Heifers During Late Peri‐Implantation Period          下载免费PDF全文

MM Meyerholz  K Mense  H Knaack  O Sandra  M Schmicke 《Reproduction in domestic animals》2016,51(1):175-177

The bovine embryonic signal interferon‐τ (IFN‐τ) produced by the trophoblast is known to pass through the uterine fluid towards the endometrium and further into the maternal blood, where IFN‐τ induces specific expression of interferon‐stimulated gene expression (ISG), for example in peripheral leucocytes. In sheep, it was shown experimentally by administration of IFN‐τ that ISG is also detectable in the liver. The objective was to test whether ISG can be detected in liver biopsy specimens from Holstein–Friesian heifers during early pregnancy. Liver biopsies were taken on day 18 from pregnant and non‐pregnant heifers (n = 19), and the interferon‐stimulated protein 15 kDa (ISG‐15) and myxovirus‐resistance protein‐1 (MX‐1) gene expression was detected. The expression of both MX‐1 (p: 24.33 ± 7.40 vs np: 9.00 ± 4.02) and ISG‐15 (p: 43.73 ± 23.22 vs 7.83 ± 3.63) was higher in pregnant compared to non‐pregnant heifers (p < 0.05). In conclusion, pregnancy induced ISG‐15 and MX‐1 gene expression in the liver already at day 18 in cattle.  相似文献   

光质和光强对红树莓组培苗叶绿素荧光参数的影响     

郭芳  王超  刘海鹏  李保国  张雪梅  齐国辉 《北方园艺》2016,(11):19-23

为了确立红树莓组培苗生长的适宜光质和光强,以红树莓"海尔特兹"组培苗为试材,研究了不同光质和光强处理下红树莓生根组培苗的叶绿素含量及叶绿素荧光参数。结果表明:光质处理中,除R/B(红光∶蓝光=1∶1)处理外,Chl a(叶绿素a)、Chl b(叶绿素b)和Ct(叶绿素总含量)均随着R/B比率的增加呈现先增加后减小的趋势,Chl a和Ct均在2R/B(红光∶蓝光=2∶1)处理下呈现最大值,分别为1.344、1.716mg·g~(-1),Chl b则在R/2B(红光∶蓝光=1∶2)处理最高,为0.423mg·g~(-1),Chl a、Chl b和Ct均以10R/B(红光∶蓝光=10∶1)处理最低,分别为0.130、0.033、0.163mg·g~(-1),均显著低于其它光质;Chl a/b(叶绿素a/b)在10R/B、2R/B和R/B处理下较高,分别为3.942、3.709、3.653,呈现阳生植物的特性,而在6R/B(红光∶蓝光=6∶1)、W(白光)、4R/B(红光∶蓝光=4∶1)等处理下较低,分别为2.729、2.730、2.747,呈现阴生植物的特性;在R/2B处理下,红树莓组培苗叶片的叶绿素荧光参数值表现极优,Fv/Fm(PSⅡ最大光化学效率)值达到0.881,而10R/B处理下,Fv/Fm值降低到0.355,且显著低于其它光质处理;光强处理中,随着光强的增加,Chl a、Chl b和Ct呈现递减的趋势,最大值由1.218、0.503、1.720mg·g~(-1)分别降低到0.907、0.302、1.209mg·g~(-1),均形成显著差异,Chl a/b则表现为随着光强的增加而增加,在2 000lx处理达到最大值,为3.042,且3种光强处理差异显著;叶片的Fo(初始荧光)随着光强的增加而增加,在2 000lx处理下达到最大值,为180.000,Fm(最大荧光值)则在1 500lx处理下达到最大值,为907.000,而叶片的Fv/Fm、Fv/Fo(PSⅡ的潜在活性)、ΦPSⅡ(PSⅡ实际光化学效率)、Rfd(可变荧光下降比值)则均随着光强的增加呈现递减的趋势,均在1 000lx处理下呈现最大值,分别为0.847、5.573、0.815、4.200。  相似文献   

不同颜色色板对脐橙果园昆虫的诱集效果     

张伟  徐家生  戴小华  郭青云  李国胜  周芳 《北方园艺》2016,(21):111-115

以脐橙果园内昆虫为调查对象,于2014年12月至2015年11月使用紫色、灰色、粉色、蓝色、青色、绿色、黄色、红色、白色和黑色等10种不同颜色的色板对脐橙果园内的昆虫进行诱集,研究了脐橙果园内昆虫群落的组成以及昆虫对不同颜色的趋向性。结果表明:10种颜色色板在脐橙果园内诱集的昆虫有10目48科54种,其中双翅目昆虫诱集数量最多,达到诱虫总数的94.81%;不同颜色色板对脐橙果园昆虫的诱集效果不同,黄色色板诱集的昆虫数量最多,其次是青色和绿色的色板,即波长450~597nm诱虫效果好;粉虱类昆虫更趋向于黄板,而实蝇类昆虫则趋向于青板和绿板;不同月份色板诱集的昆虫个体数存在明显差异。  相似文献   

应用压力早期恢复资料确定低渗地层压力   总被引：3，自引：0，他引：3  

魏芳 《长江大学学报》2007,4(3):42-43,52

低渗透油藏的压力恢复曲线,不能用Horner外推压力求取地层压力。基于Horner方程的等轴双曲线形式,提出了应用压力早期恢复资料确定低渗地层压力的新方法。通过油井实际资料计算验证,用该方法计算的地层压力准确可靠,较好地解决了低渗透油藏求地层压力的问题。  相似文献   

Morphologic and biochemical characterization of hyperextension of the metacarpophalangeal and metatarsophalangeal joints in llamas     

Reed SK  Semevolos SA  Rist PK  Valentine BA 《American journal of veterinary research》2007,68(8):879-885

OBJECTIVE: To determine the morphologic and biochemical characteristics of hyperextension of the metacarpophalangeal and metatarsophalangeal joints in llamas. ANIMALS: 12 adult llamas (6 with bilateral hyperextension of the metacarpophalangeal or metatarsophalangeal joints and 6 age- and sex-matched control llamas). PROCEDURES: Llamas were evaluated by use of lameness examination, ultrasonography, and radiography. A CBC, serum biochemical analysis, and determination of concentrations of trace minerals in serum and liver samples were performed. Llamas were euthanized, and samples of the superficial digital flexor tendon, deep digital flexor tendon, and suspensory ligament were obtained from 4 areas and snap-frozen in liquid nitrogen or suspended in neutral-buffered 10% formalin. Immunohistochemical evaluation of collagen types I and III and assays for measurement of lysyl oxidase activity were performed. RESULTS: 2 affected llamas had a visible gait deficit associated with metacarpophalangeal joint hyperextension. Radiographic evidence of osteoarthritis was detected in 1 severely affected llama, and ultrasonographic changes of soft tissue mineralization and suspensory desmitis were observed in 2 llamas. Liver concentrations of copper were lower and serum concentrations of zinc higher in affected llamas, compared with values in control llamas. Lysyl oxidase activity and collagen distribution did not differ significantly between groups. CONCLUSIONS AND CLINICAL RELEVANCE: Hyperextension of the metacarpophalangeal or metatarsophalangeal joints in llamas does not appear to be the result of injury or degeneration of the suspensory ligament or flexor tendons. Lower copper concentrations coupled with higher zinc concentrations in affected llamas may be indicative of secondary copper deficiency.  相似文献   

Influx of neutrophils and persistence of cytokine expression in airways of horses after performing exercise while breathing cold air     

Davis MS  Williams CC  Meinkoth JH  Malayer JR  Royer CM  Williamson KK  McKenzie EC 《American journal of veterinary research》2007,68(2):185-189

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