全文获取类型
收费全文 | 305篇 |
免费 | 22篇 |
专业分类
林业 | 22篇 |
农学 | 8篇 |
基础科学 | 3篇 |
71篇 | |
综合类 | 40篇 |
农作物 | 17篇 |
水产渔业 | 21篇 |
畜牧兽医 | 88篇 |
园艺 | 30篇 |
植物保护 | 27篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 19篇 |
2019年 | 11篇 |
2018年 | 15篇 |
2017年 | 11篇 |
2016年 | 16篇 |
2015年 | 6篇 |
2014年 | 14篇 |
2013年 | 18篇 |
2012年 | 22篇 |
2011年 | 28篇 |
2010年 | 20篇 |
2009年 | 10篇 |
2008年 | 21篇 |
2007年 | 22篇 |
2006年 | 10篇 |
2005年 | 13篇 |
2004年 | 9篇 |
2003年 | 8篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1969年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有327条查询结果,搜索用时 609 毫秒
321.
Studt F Abild-Pedersen F Bligaard T Sørensen RZ Christensen CH Nørskov JK 《Science (New York, N.Y.)》2008,320(5881):1320-1322
The removal of trace acetylene from ethylene is performed industrially by palladium hydrogenation catalysts (often modified with silver) that avoid the hydrogenation of ethylene to ethane. In an effort to identify catalysts based on less expensive and more available metals, density functional calculations were performed that identified relations in heats of adsorption of hydrocarbon molecules and fragments on metal surfaces. This analysis not only verified the facility of known catalysts but identified nickel-zinc alloys as alternatives. Experimental studies demonstrated that these alloys dispersed on an oxide support were selective for acetylene hydrogenation at low pressures. 相似文献
322.
Torres-Rosell J De Piccoli G Cordon-Preciado V Farmer S Jarmuz A Machin F Pasero P Lisby M Haber JE Aragón L 《Science (New York, N.Y.)》2007,315(5817):1411-1415
Cellular checkpoints prevent mitosis in the presence of stalled replication forks. Whether checkpoints also ensure the completion of DNA replication before mitosis is unknown. Here, we show that in yeast smc5-smc6 mutants, which are related to cohesin and condensin, replication is delayed, most significantly at natural replication-impeding loci like the ribosomal DNA gene cluster. In the absence of Smc5-Smc6, chromosome nondisjunction occurs as a consequence of mitotic entry with unfinished replication despite intact checkpoint responses. Eliminating processes that obstruct replication fork progression restores the temporal uncoupling between replication and segregation in smc5-smc6 mutants. We propose that the completion of replication is not under the surveillance of known checkpoints. 相似文献
323.
Cinthya?Zorrilla Felix?Navarro Sandra?Vega John?Bamberg Jiwan?PaltaEmail author 《American Journal of Potato Research》2014,91(6):673-687
Developing chipping cultivars with improved tuber quality and disease resistance is a major interest for breeders and the potato industry. A popular chipping cultivar ‘Atlantic’, is desired for its high yield and gravity. However, this cultivar suffers from poor internal tuber quality and high scab susceptibility. On the contrary, cultivar ‘Superior’ is known to have excellent tuber internal quality and moderately scab resistance. In addition, this cultivar is known to have high tuber calcium as compared to ‘Atlantic’. The present study intended to generate populations that can be suitable for the genetic study of tuber calcium, internal quality, common scab, and other commercially important traits such as yield, specific gravity and chip quality at the tetraploid level. Two populations obtained by reciprocally crossing the cultivars ‘Atlantic’ and ‘Superior’ were evaluated during 2009 to 2012 at Hancock, Wisconsin. Significant genotype effects and moderately low to high broad-sense heritabilities were identified for all traits evaluated indicating that the observed phenotypic variation has an important genetic component. In addition, the parents differed significantly for all traits across trials, and most genotypes performed in between the two parents but some genotypes were more extreme than the parents. Furthermore, evidence of reciprocal effects was found for some traits. In addition to learning about the genetics of these important traits we were able to identify some genotypes that combined the commercially desired traits of the two cultivars. 相似文献
324.
McAnuff MA Omoruyi FO Sotelo-López A Asemota HN 《Plant foods for human nutrition (Dordrecht, Netherlands)》2005,60(2):93-98
Two wild (Dioscorea polygonoides and Rajana cordata) and seven cultivated varieties of Jamaican yams (Dioscorea spp.) were analyzed for their proximate composition and the levels of antinutritional factors. The protein level range was 47.8 ± 2.6 to 88.0 ± 2.5 g/kg dry weight. The lowest level was seen in D. cayenensis. The range for the dietary fiber content in the tubers was 16.3 ± 0.7 to 63.5 ± 0.4 g/kg dry weight. The wild yam varieties recorded higher levels. Saponins level was < 600 mg/kg dry weight in all the tubers analyzed except for bitter yam (2962.5 ± 60.5 mg/kg dry weight). Total phenol content ranged from 1.3 ± 0.1 to 79.3 ± 6.1 g/kg while total condensed tannin content ranged from 0.1 ± 0.0 to 26.7 ± 3.8 g/kg dry weight. Samples that showed high levels of phenols also had high levels of condensed tannins. All the samples analyzed contained low levels of lectins and no alkaloids were detected. The levels of antinutritional factors did not clearly delineate the wild varieties from the edible varieties. 相似文献
325.
Mbulu RS Tjipura-Zaire G Lelli R Frey J Pilo P Vilei EM Mettler F Nicholas RA Huebschle OJ 《Veterinary microbiology》2004,98(3-4):229-234
A study was carried out on four adult cattle to assess the pathogenicity of Mycoplasma mycoides subsp. mycoides SC strain T1/44, currently used as a vaccine for the control of contagious bovine pleuropneumonia (CBPP) in Namibia. Post mortem examination 9 weeks after endobronchial inoculation of the vaccine strain to three of the four animals revealed unilateral pleuropneumonic lesions, pleuritis and well-developed sequesters in two of the three inoculated animals and several small sequesters surrounded by pleuropneumonic lesions in the diaphragmatic and apical lobes in one animal. The fourth animal, which was not directly inoculated but was in close contact with the inoculated animals, revealed only an adhesion area of the lung to the ribcage. Serological examination carried out using the complement fixation test (CFT) detected positive titres in all three intubated animals and the indirect CBPP-LppQ-ELISA was positive for two of the three inoculated animals. The contact animal showed no seroconversion. M. mycoides subsp. mycoides SC was isolated from the sequesters of two of the inoculated animals. Isolation of mycoplasmas was not possible from the third inoculated animal due to heavy contamination of the samples by other bacteria, but the presence of M. mycoides subsp. mycoides SC could be evidenced by PCR from clinical samples. The identity of the T1/44 vaccine strain isolated from the sequesters of two animals was confirmed by T1/44-specific PCR analysis and by IS1296 typing using Southern blot. These results clearly show that inoculation of T1/44 vaccine via the endobronchial route can lead to CBPP. 相似文献
326.
Leonardo Luiz Calado Luiz Antônio Carlos Bertollo Gideão Wagner Werneck Felix da Costa Wagner Franco Molina 《Aquaculture Research》2013,44(5):829-835
Fish of the family Gerreidae, mainly species of the genera Diapterus and Eugerres, have high potential for cultivation, because of their saline tolerance. A detailed cytogenetic analysis of Diapterus auratus, Diapterus rhombeus and Eugerres brasilianus was conducted using conventional staining, C‐banding, Ag‐NOR, AT/GC‐specific fluorochrome staining and mapping of ribosomal sequences with 5S and 18S rDNA probes. All the species exhibited symmetrical karyotype, 2n = 48 acrocentric chromosomes. Ag‐NORs and 18S rDNA are present in the interstitial position on pair 1 (genus Diapterus) and pair 6 in Eugerres brasilianus. The 5S rDNA sites, located in the interstitial position (pair 11), are conserved in the three species. Heterochromatic regions are similar in the Diapterus species, showing a pattern of reduced and centromeric bands, differing from E. brasilianus, where, in addition to these, more prominent interstitial bands were observed. GC‐rich regions are located at ribosomal sites. Karyotypic comparison between Diapterus and Eugerres reveals similarity in chromosomal macrostructure, differing in C‐positive heterochromatin distribution and position of 18S sites, indicating the occurrence of structural microrearrangements. Although complementary analyses are needed, the similarities observed for these and other species suggest the possibility of breaking postzygotic barriers and their potential use, through induced interspecific or intergeneric hybridizations. 相似文献
327.
Pitch canker on plantation-grown Pinus species, caused by the fungal pathogen Fusarium circinatum, first appeared in the western and southern Cape regions of South Africa. However, outbreaks have subsequently been reported from the major plantation growing regions of KwaZulu-Natal and Limpopo in the eastern, summer rainfall regions of the country. It is more than 10 years since the last detailed population genetics studies on F. circinatum in the region were conducted. To shed light on the population biology of F. circinatum in this region of South Africa, we used microsatellite markers and mating-type assays to study a collection of 296 isolates from different nurseries and plantation sites. Our results showed that populations in the region are highly diverse, but strongly interconnected, with various genotypes shared across nursery and plantation collection sites. In contrast to nursery populations, those associated with pitch canker outbreaks were characterized by the presence of relatively small numbers of dominant genotypes that were generally widespread across the region. Opposite mating-type individuals occurred in most of the isolate collections, but multilocus linkage disequilibrium analyses pointed towards clonality being the main reproductive mode of F. circinatum in the region. Most of the pathogen's genetic variation could probably have resulted from multiple different introductions into the country and more specifically, into the summer rainfall region. Because the spread and establishment of invasive pathogens are typically driven by aggressive clones, the results of this study provide important considerations for current and future Pinus disease management strategies. 相似文献