Effects of deltamethrin on hematological parameters and enzymatic activity in Ancistrus multispinis (Pisces, Teleostei)     

C.T. Pimpão  H.C. Silva de Assis 《Pesticide biochemistry and physiology》2007,88(2):122-127

Deltamethrin (DM) is a pyrethroid insecticide widely used in Brazilian crops and in pest-control programs because of its low environmental persistence and toxicity. It has been shown to exert a wide range of effects on non-targeted organisms including fishes. The aim of this study to evaluate the effects of deltamethrin through the hematological and biochemical parameters using Ancistrus multispinis as animal model. First, blood of A. multispinis was collected by cardiac puncture in order to evaluate the basal values of the total cell counts, hemoglobin and hematocrit rates. A. multispinis showed low number leukocytes, red blood cells and hemoglobin compared to other species. The intoxication with DM (0.1 or 0.3 mg kg⁻¹) induced leukocytosis and increases the number of erythrocytes and hemoglobin levels 96 h after the injection when compared to vehicle-injected animals. The injection of lipopolysaccharide (LPS) also induced a significant leukocytosis and increased the erythrocytes number 4 h after the injection. The combination of the higher dose of DM with LPS showed an additional effect in the number of leukocytes and erythrocytes. The GST and EROD activities were also evaluated in the gills and liver, respectively, 96 h after intoxication with both doses of DM. The GST activity decreased after DM intoxication in both doses while EROD activity was not significantly different. These results suggest that A. multispinis is a valuable model for ecotoxicological studies. Also, DM can affect the metabolizing system (GST) and immunologic system of A. multispinis, a condition that can be worsened in the presence of inflammation or infection.  相似文献   

Reaction of cucurbits species to infection with Zucchini lethal chlorosis virus     

J.S. Giampan  J.A.M. RezendeR.F. Silva 《Scientia Horticulturae》2007

Zucchini lethal chlorosis virus (ZLCV) is a tospovirus that infects species of cucurbits in Brazil and is transmitted by the thrips Frankliniella zucchini. The purpose of the present work was to evaluate the reaction of seven species of cucurbits to infection with ZLCV under field and greenhouse conditions in Piracicaba County, SP. In the field experiment, ZLCV infection occurred naturally. In the greenhouse, plants were mechanically inoculated with ZLCV at the cotyledonal stage. Evaluations were based on symptoms expression and detection of the virus by PTA-ELISA. The percentages of infected plants for field and greenhouse assays, respectively, are indicated in parenthesis for each species/cultivar: Cucurbita pepo var. Caserta (72.9 and 70.7); C. maxima var. Alice (16.0 and 10.4); C. maxima var. Exposição (0.0 and 0.0); C. moschata var. Menina Brasileira (29.2 and 18.2); C. maxima × C. moschata hybrid Takaiama (63.4 and 42.7); Citrullus lanatus var. Crimson Sweet (25.0 and 25.0); Cucumis sativus var. Safira (14.3 and 41.2); and C. anguria (21.4 and 22.7).  相似文献   

Ineffectiveness of pruning to control citrus huanglongbing caused by <Emphasis Type="Italic">Candidatus</Emphasis> Liberibacter americanus     

Silvio Aparecido Lopes  Guilherme Fernando Frare  Pedro Takao Yamamoto  Antonio Juliano Ayres  José Carlos Barbosa 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):463-468

The huanglongbing (HLB) disease of citrus trees, caused by Candidatus Liberibacter asiaticus and Ca. Liberibacter americanus, was first reported in Brazil in March, 2004. The presence of the disease has caused serious concerns among growers. Pruning experiments were conducted to determine if removal of symptomatic branches or the entire canopy (decapitation) would eliminate infected tissues and save HLB-affected trees. Pruning was done in five blocks on a total of 592 3- to 16 year-old ‘Valência’, ‘Hamlin’ or ‘Pêra’ sweet orange trees showing no symptoms or with two levels of symptom severity. Ten decapitated trees per block were caged and all trees were treated with insecticides to control the psyllid vector, Diaphorina citri. Mottled leaves reappeared on most symptomatic (69.2%) as well on some asymptomatic (7.6%) pruned trees, regardless of age, variety, and pruning procedure. Presence of the pathogen (Ca. Liberibacter americanus) in all symptomatic trees was confirmed by PCR. In general, the greater the symptom severity before pruning the lower the percentage of trees that remained asymptomatic after pruning.  相似文献   

Molecular, physiological and pathological characterization of Corynespora leaf spot fungi from rubber plantations in Sri Lanka   总被引：4，自引：0，他引：4  

W. P. K. Silva  B. J. Deverall  & B. R. Lyon 《Plant pathology》1998,47(3):267-277

The plant pathogenic fungus Corynespora cassiicola causes a severe leaf spot disease on more than 70 host plant species including Hevea brasiliensis . Genetic variability in 32 isolates of C. cassiicola collected from diverse hosts and locations in Sri Lanka and Australia was assessed using restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region of ribosomal DNA and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis of total fungal DNA. Amplified ITS fragments from all 32 C. cassiicola isolates exhibited an identical size, and restriction analysis with seven different restriction endonucleases revealed identity in all of the detected DNA fragments. This finding of high genetic relatedness was further supported by the cloning and DNA sequencing of the ITS2 region from one Sri Lankan and one Australian isolate. However, RAPD-PCR profiles generated by 15 oligonucleotide decamer primers revealed significant polymorphism between groups of organisms. Genetic relationships among the isolates were determined by cluster analysis of the RAPD-PCR data and seven different RAPD groups were identified. Isolates showed strong correlations between the assigned RAPD group and the location and host plant genotype from which the isolate was collected. Correlations were also observed between the RAPD group, growth of the isolate and pathogenicity on different plant hosts.  相似文献   

Biological control ofBotrytis cinerea in residues and flowers of Rose (Rosa hybrida)     

Joseli da Silva Tatagiba  Luiz Antonio Maffia  Roberto W. Barreto  Acelino C. Alfenas  John C. Sutton 《Phytoparasitica》1998,26(1):8-19

Microbial isolates from living petals, petal residues and leaf residues of rose, and from laboratory collections, were evaluated for control ofBotrytis cinerea in rose. In leaf residues artificially infested withB. cinerea, isolates of the filamentous fungiGliocladium roseum, FR136 (unidentified) andTrichoderma inhamatum reduced sporulation of the pathogen by >90%, other filamentous fungi were 25–90% effective, and those of yeasts and bacteria were <50% effective. In artificially inoculated petal residues, no microbe reduced sporulation ofB. cinerea by >75%, but isolates ofCladosporium oxysporum and four yeasts were 51–75% effective, and three filamentous fungi, eight yeasts andBacillus subtilis isolates were 26–50% effective. Isolates ofT. inhamatum, C. oxysporum andG. roseum performed best againstB. cinerea among isolates evaluated in leaf residues naturally infested with the pathogen and indigenous microorganisms. Totals of ten isolates of filamentous fungi (includingC. oxysporum andC. cladosporioides), two of yeasts and five ofBacillus subtilis completely prevented lesion production byB. cinerea in detached petals, and a further six isolates of filamentous fungi (includingG. roseum) and six yeasts were 90–99% effective. Isolates ofC. oxysporum, C. cladosporioides andB. subtilis, the most effective microorganisms againstB. cinerea in flower buds, reduced number of lesions in the range of 42–65% compared with 59–89% for à standard fungicide (vinclozolin). It is suggested that application of leading antagonists Jo living rose leaves and flowers should optimize control of inoculum production byB. cinerea when the tissues die. Optimal biocontrol of lesion production in flower buds requires a better understanding of the microenvironment of petals.  相似文献   

Evaluation of Feed Attractants in Juvenile Senegalese Sole,Solea senegalensis     

Fernando G. Barroso  Ana Rodiles  Antonio J. Vizcaino  Tomás F. Martínez  Francisco J. Alarcón 《Journal of the World Aquaculture Society》2013,44(5):682-693

The Senegalese sole, Solea senegalensis, is a flatfish with increasing interest in the European aquaculture. However, one of the main limitations found in this species is related to the scarce attraction to commercial aquafeeds, thus determining low feed intake in fish farms. The aim of this study was to evaluate four ingredients of marine origin (fish meal hydrolysate [FMH], polychaete meal [POM], mussel meal [MUM], and squid meal [SQM]) as potential attractants for juvenile Senegalese sole. Four experimental feeds containing 10% of the potential attractants, plus a control diet without these ingredients, were evaluated. A total of 150 fish were distributed into ten 40‐L tanks (duplicate tanks per feed). A Latin square design was carried out, and hence all the experimental diets were offered to each tank in successive periods of 7 d. Fish were fed twice a day (morning and afternoon), and the feeding behavior of the animals was assessed by direct observations. Behavioral pattern assessed was classified as distant orientation, near orientation, or continuation of intake. The acceptability index for each behavioral phase and experimental feeds used was established for each observation. No behavioral differences could be noticed between the morning and the afternoon feedings. FMH showed the greatest attractant potential than among the ingredients assessed, and consequently this ingredient yielded the higher feed intake rates. On the other hand, POM was not the best attractant for juvenile Senegalese sole.  相似文献   

Resistance of 57 greenhouse-grown accessions of Lycopersicon esculentum and three cultivars to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)     

Fabrício Alves Oliveira  Derly José Henriques da Silva  Germano Leão Demolin Leite  Gulab Newandram Jham  Marcelo Picanço 《Scientia Horticulturae》2009

The aim of this study was to evaluate resistance to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) by antixenosis on 57 Lycopersicon esculentum Mill. accessions from the Horticultural Germplasm Bank (HGB) of Universidade Federal de Viçosa and by the three commercial cultivars (Santa Clara, Moneymaker and TOM-601) under greenhouse conditions. A randomized complete block design was used with three replications. Infestations with T. absoluta adults were performed weekly and the following characteristics were evaluated: number of small, large and total mines/leaf and % of leaves mined at days 60, 75 and 90 after planting. Low infestation occurred at days 60 and 75 but at day 90, infestation was sufficient to evaluate insect damage. Based on these data it was concluded that only accessions HGB-674 and HGB-1497 appeared to be the most promising. In addition, to determine possible chemical causes of resistance, hexane extracts were analyzed at day 90 by gas chromatography/mass spectrometry and the major peaks identified by a mass spectral database using similarity index. Nine hydrocarbons, viz., hexadecane, heptadecane, eicosane, tricosane, 2-methyltricosane, tetracosane, hexacosane, octacosane and triacontane were identified in the hexane extracts in many samples. Tricosane, tetracosane and hexacosane presented significant correlations with the leaves mined. Only tricosane presented a negative correlation with the number of small mines (r = −0.28), total number of mines (r = −0.27) and % of leaves mined (r = −0.22). However, tetracosane and hexacosane presented significant positive correlations (r = 0.25 and 0.24, respectively) with the % of leaves mined.  相似文献   

Evaluation of hematological,biochemical and oxidative stress profile in calves under propofol anesthesia     

Lima  Pedro Paulo Arcanjo  Narciso  Luis Gustavo  Alcindo  Jefferson Filgueira  Deschk  Maurício  Ciarlini  Paulo Cesar  dos Santos  Paulo Sérgio Patto  de Almeida  Breno Fernando Martins 《Veterinary research communications》2022,46(1):27-35

Veterinary Research Communications - Propofol is a widely used drug in veterinary medicine to induce anesthesia; as well as the chosen compound for protocols of intravenous anesthesia. The present...  相似文献   

Dynamics of natural infection by Toxoplasma gondii in goat herds in the semiarid region of northeastern Brazil: transitional antibody observation     

Sousa  Larissa Nascimento  Batista  Samira Pereira  Silva  Samara Santos  da Silva  Rômulo Fylipe  Sarmento  Wlysse Ferreira  Mota  Rinaldo Aparecido  Brasil  Arthur Willian Lima  Amaku  Marcos  Feitosa  Thais Ferreira  Vilela  Vinícius Longo Ribeiro 《Veterinary research communications》2022,46(2):507-515

Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the...  相似文献   

Antibacterial activity of Persea cordata stem barks     

Schlemper SR  Schlemper V  da Silva D  Cordeiro F  Cruz AB  Oliveira AE  Cechinel-Filho V 《Fitoterapia》2001,72(1):73-75

The antibacterial effects of extracts obtained from Persea cordata stem bark, employed in Brazil to treat infectious diseases, were studied. The ethyl acetate fraction of the hydroalcoholic extract showed activity against pathogenic bacteria which may justify the popular use of the plant.  相似文献